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Integrated Assessments of Potential Risks to Aquatic Organisms and Public Water Supply from Wastewater-Derived Chemical Mixtures in the Chesapeake Bay Watershed
Toxic contaminants harm fish and wildlife in the Chesapeake Bay and pose risks to human health.
Proper management of these contaminants requires scientific efforts to understand their occurrence, sources, environmental fate, efficacy of removal through wastewater treatment, biological effects, and the human health impacts of exposure.
Highlighted Web Tool
Where are discharges of treated wastewater likely to affect water quality and aquatic life in the Potomac River watershed?
Proper management of contaminants of emerging concern in the Chesapeake Bay region requires scientific efforts to understand the risk posed to aquatic resources from the “cocktail” of multiple contaminants that is often present. This research aims to assess the occurrence, sources, environmental impacts, biological effects, and the human health impacts of toxic contaminants in rivers.
Highlighted Report: Wastewater Reuse and Predicted Ecological Risk Posed by Contaminant Mixtures in Potomac River Watershed Streams
This study applied a wastewater model to the Potomac River watershed to identify streams with a high likelihood of carrying elevated effluent-derived contaminants. Results were used in a screening-level risk assessment to evaluate the potential risks to aquatic life and drinking water posed by municipal wastewater-contaminant mixtures.
Highlighted Report: Watershed-Scale Risk to Aquatic Organisms From Complex Chemical Mixtures in the Shenandoah River
This study examined the potential and observed ecological risk posed by chemical mixtures in the Shenandoah River watershed, using a holistic approach to evaluate landscape, hydrological, chemical, and biological variables.
Major findings of efforts in the Shenandoah and Potomac River watersheds suggest that:
Wastewater reuse may be a significant source of contaminant loading to rivers
Complex chemical mixtures are widespread, resulting in both predicted and observed risks to aquatic organisms
De facto wastewater reuse (the presence of treated wastewater in drinking water sources) may negatively affect public water supplies
These findings have water resource management implications that this project will explore further in cooperation with the Chesapeake Bay Program, with the ultimate goal of reducing the impact of toxic contaminants to protect aquatic ecosystem health for generations to come.
The Chesapeake Bay and its watershed is one of America’s greatest natural treasures. However, toxic contaminants introduced into the watershed from sources such as industrial waste, municipal wastewater discharges, agricultural runoff, and stormwater runoff can harm the living resources that call the Bay home. Human health can also be affected, such as through impacts to drinking water quality or consumption of contaminated animal tissues.
One of the Chesapeake Bay Program’s goals is to reduce the impact of these contaminants on the Bay and its surrounding areas. To achieve this goal, the USGS has been leading several research efforts to: (1) gain insights into the sources, fate, and effects of toxic contaminants in the Chesapeake Bay watershed and (2) inform efforts to mitigate their harmful effects.
Scientists deployed mobile fish exposure laboratories at sites in the Shenandoah River watershed, Virginia, to understand exposure and effects on fish. This photo shows the aquariums in a laboratory where fish were exposed to stream water or wastewater.
The USGS Environmental Health Mission Area's Toxic Substances Hydrology Program has conducted several integrated studies in coordination with the Chesapeake Bay Program to better understand the occurrence and fate of toxic contaminants in surface waters, with much focus on contributions from contaminant loading from treated wastewater.
Treated wastewater is water that has been processed through physical, chemical, or biological processes to remove contaminants before being discharged into rivers and other bodies of water. This reuse of treated wastewater is an essential component of the modern water cycle that can help maintain flow of water in rivers during dry periods. Many emerging contaminants, however, are not fully removed by conventional wastewater treatment technologies. These contaminants, once discharged into the environment, may have harmful effects on aquatic life and human health.
Early studies in the Shenandoah identified a number of constituents derived from natural and anthropogenic sources in the river waters sampled, including pharmaceuticals, personal care products, pesticides, hormones, disinfection by-products, and fossil fuel combustion products. Although more than 550 individual constituents were found, these represent only a small fraction of the potential contaminants present in the water. Since then, holistic integrated approaches have been developed to assess the perceived versus actual risk posed to aquatic organisms and human health from exposure to these complex chemical mixtures derived from water reuse and other sources in the Shenandoah as well as the broader Potomac River watershed, of which the Shenandoah is a subbasin.
A fundamental challenge in conducting integrated transdisciplinary research is linking continental-scale issues to molecular-scale processes. This requires the ability to upscale or downscale to address a particular study need while maintaining the integration of landscape, hydrological, chemical, and biological processes.
The approaches used by this project to address these challenges include:
Documenting the effects of streamflow and landscape variables on contaminant concentrations and determining the relations to the cumulative loadings from wastewater treatment plants. This involved developing an accumulated wastewater ratio model for the Shenandoah River watershed that: (1) accounts for upstream contributions of all municipal and industrial effluent discharges and (2) calculates the percentage of wastewater for each stream in the watershed.
Determining the spatial and temporal concentration and composition patterns of 273 constituents detected in water sampled collected in the Shenandoah River watershed between 2014 and 2016.
Conducting on-site mobile laboratory exposure studies to evaluate the relations between fish endocrine disruption and endocrine-disrupting chemicals in both streams and municipal wastewater effluent. These experiments applied and refined protocols developed at wastewater treatment plants and impacted streams by other studies (Barber et al., 2007; Vajda et al., 2011; Barber et al., 2012).
Focusing on effects of complex chemical mixture exposure on physiological, molecular, and multi-omics pathways for the model organism fathead minnow.
Associated report currently in review: Bertolatus, D.W., Barber, L.B., Martyniuk, C.J., Zhen, H, Collette, T.W., Ekman, D.R., Jastrow, A., Rapp, J.L., and Vajda, A.M., 2022 – in review, Watershed-scale integrated ecotoxicological assessment: Multi-omic responses of fathead minnows exposed to complex chemical mixtures. To be submitted to Science of the Total Environment.
Focusing on effects of de facto wastewater reuse on disinfection byproduct formation in public water supply systems.
Abbott, B.W., Bishop, K., Zarnetske, J.P., Minaudo, C., Chapin, F.S., III, Krause, S., Hannah, D.M., Conner, L., Ellison, D., Godsey, S.E., Plont, S., Marcais, J., Kolbe, T., Huebner, A., Frei, R.J., Hampton, T., Gu, S., Buhman, M., Sayedi, S.S., Ursache, O., Chapin, M., Henderson, K.D., and Pinay, G., 2019, Human domination of the global water cycle absent from depictions and perceptions. Nature Geoscience, v. 12, p. 533-540.
Barber, L.B., Lee, K.E., Swackhamer, D.L., and Schoenfuss, H.L., 2007, Reproductive responses of male fathead minnows exposed to wastewater treatment plant effluent, effluent treated with XAD8 resin, and an environmentally relevant mixture of alkylphenol compounds. Aquatic Toxicology, v. 82, p. 36-46.
Barber, L.B., Vajda, A.M., Douville, C., Norris, D.O., and Writer, J.H., 2012, Fish endocrine disruption responses to a major wastewater treatment facility upgrade. Environmental Science and Technology, v. 46, p. 2121-2131.
Bergman, A., Heindel, J.J., Jobling, S., Kidd, K.A., and Zoeller, R.T., 2013, State of the Science of Endocrine Disrupting Chemicals – 2012. World Health Organization, Geneva, Switzerland.
Blazer, V.S., Iwanowicz, L.R., Henderson, H., Mazik, P.M., Jenkins, J.A., Alvarez, D.A., and Young, J.A., 2012, Reproductive endocrine disruption in smallmouth bass (Micropterus dolomieu) in the Potomac River basin: spatial and temporal comparisons of biological effects. Environmental Monitoring and Assessment, v. 184, p. 4309-4334.
Bradley, P.M., Journey, C.A., Romanok, K.M., Barber, L.B., Buxton, H.T., Foreman, W.T., Furlong, E.T., Glassmeyer, S.T., Hladik, M.L., Iwanowicz, L.R., Jones, D.K., Kolpin, D.W., Kuivila, K.M., Loftin, K.A., Mills, M.A., Meyer, M.T., Orlando, J.L., Reilly, T.J., Smalling, K.L., and Villeneuve, D.L., 2017, Expanded target-chemical analysis reveals extensive mixed-organic-contaminant exposure in USA streams. Environmental Science and Technology, v. 51, p. 4792-4802.
Dieter, C.A., Maupin, M.A., Caldwell, R.R., Harris, M.A., Ivahnenko, T.I., Lovelace, J.K., Barber, N.L., and Linsey, K.S., 2018, Estimated use of water in the United States in 2015. U.S. Geological Survey Circ. 1441: U.S. Geological Survey; Washington, DC. Accessed March 2020 at [https://doi.org/10.3133/cir1441].
Gordon, S., Jones, D.K., Blazer, V.S., Iwanowicz, L., Williams, B., and Smalling, K., 2021, Modeling estrogenic activity in streams throughout the Potomac and Chesapeake Bay watersheds. Environmental Monitoring and Assessment, v. 193, Article number 105.
Kolpin, D.W., Furlong, E.T., Meyer, M.T., Thurman, E.M., Zaugg, S.D., Barber, L.B., and Buxton, H.T., 2002, Pharmaceuticals, hormones, and other organic wastewater contaminants in U.S. streams, 1999-2000 - A national reconnaissance. Environmental Science and Technology, v. 36, p. 1202-1211.
Kolpin, D.W., Blazer, V.S., Gray, J.L., Focazio, M.J., Young, J.A., Alvarez, D.A., Iwanowicz, L.R., Foreman, W.T., Furlong, E.T., Speiran, G.K., Zaugg, S.D., Hubbard, L.E., Meyer, M.T., Sandstrom, M.W., and Barber, L.B., 2013, Chemical contaminants in water and sediment near fish nesting sites in the Potomac River Basin: Determining potential exposures to smallmouth bass (Micropterus dolomieu). Science of the Total Environment, v. 443, p. 700-716.
Masoner, J.R., Kolpin, D.W., Cozzarelli, I.M., Barber, L.B., Burden, D.S., Foreman, W.T., Forshay, K.J., Furlong, E.T., Groves, J.F., Hladik, M.L., Hopton, M.E., Jaeschke, J.B., Keefe, S.H., Krabbenhoft, D.P., Lowrance, R., Romanok, K.M., Rus, D.L., Selbig, W.R., Williams, B.H., and Bradley, P.M., 2019, Urban stormwater: An overlooked pathway of extensive mixed contaminants to surface and groundwaters in the United States. Environmental Science and Technology, v. 53, p. 10070-10081.
National Research Council, 2012, Water Reuse: Potential for Expanding the Nation’s Water Supply through Reuse of Municipal Wastewater. National Academies Press, Washington, DC.
United Nations World Water Assessment Programme, 2017, Wastewater – The Untapped Resource. The United Nations World Water Development Report 2017, UNESCO, Paris, France.
U.S. Environmental Protection Agency, 2020, National water reuse action plan: Improving the security, sustainability, and resilience of our nation’s water resources. U.S. Environmental Protection Agency Collaborative Implementation, Version 1: U.S. Environmental Protection Agency; Washington, DC, 40 p. plus Appendices.
Vajda, A.M., Barber, L.B., Gray, J.L., Lopez, E.M., Bolden, A.M., Schoenfuss, H.L., and Norris, D.O., 2011, Demasculinization of male fish by wastewater treatment plant effluent. Aquatic Toxicology, v. 103, p. 213-221.
Wastewater is used water. It includes substances such as human waste, food scraps, oils, soaps and chemicals. In homes, this includes water from sinks, showers, bathtubs, toilets, washing machines and dishwashers. Businesses and industries also contribute their share of used water that must be cleaned.
Wastewater is used water. It includes substances such as human waste, food scraps, oils, soaps and chemicals. In homes, this includes water from sinks, showers, bathtubs, toilets, washing machines and dishwashers. Businesses and industries also contribute their share of used water that must be cleaned.
Issue: Municipal and industrial wastewater effluent is an important source of water for streams and rivers, especially during periods of low flow. The reuse of wastewater effluent may become even more important if climate change exacerbates low streamflow and drought conditions. However, wastewater effluent often contains chemicals that, when chronically present, can affect the health of aquatic...
Wastewater reuse may be detrimental to smallmouth bass abundance in the Shenandoah River Watershed
Issue: Municipal and industrial wastewater effluent is an important source of water for streams and rivers, especially during periods of low flow. The reuse of wastewater effluent may become even more important if climate change exacerbates low streamflow and drought conditions. However, wastewater effluent often contains chemicals that, when chronically present, can affect the health of aquatic...
A key component to assessing the contaminant exposure pathways in streams and rivers of the Chesapeake Bay is using GIS-based landscape analysis to identify sources of endocrine disrupting chemicals. Municipal and industrial wastewater treatment plant (WWTP) discharges are potentially major sources of EDCs to streams, and therefore understanding the de facto wastewater reuse (represented as...
GIS-based landscape analysis to identify sources of endocrine disrupting chemicals
A key component to assessing the contaminant exposure pathways in streams and rivers of the Chesapeake Bay is using GIS-based landscape analysis to identify sources of endocrine disrupting chemicals. Municipal and industrial wastewater treatment plant (WWTP) discharges are potentially major sources of EDCs to streams, and therefore understanding the de facto wastewater reuse (represented as...
De facto wastewater reuse from Waste Water Treatment Facilities (WWTF) has the potential to be a significant contributor of Endocrine Disrupting Chemicals. An ArcGIS model of WWTFs, NHDPlus Version 2 stream networks (USGS and EPA 2012), and gage stations across the Shenandoah River watershed was created to calculate accumulated wastewater ratio. Virginia Pollutant Discharge Elimination...
This data release presents chemical and biological results from investigations of water quality, fish endocrine disruption, and emergent insects in the Shenandoah River Watershed (Virginia and West Virginia, USA) conducted during 2014, 2015, and 2016. Multiple sampling campaigns were conducted at sites located throughout the Shenandoah River Watershed (Table 1). The complex inorganic and...
Mobile Fish Exposure Laboratory on Site in the Shenandoah River Waters
One of the mobile fish exposure laboratories that were deployed at sites in the Shenandoah River watershed, Virginia. Fish were housed in the mobile laboratories and exposed to stream water or wastewater during the study to understand exposure and effects on fish.
One of the mobile fish exposure laboratories that were deployed at sites in the Shenandoah River watershed, Virginia. Fish were housed in the mobile laboratories and exposed to stream water or wastewater during the study to understand exposure and effects on fish.
Aquariums Inside a Mobile Fish Exposure Laboratory
Scientists deployed mobile fish exposure laboratories at sites in the Shenandoah River watershed, Virginia, to understand exposure and effects on fish. This photo shows the aquariums in a laboratory where fish were exposed to stream water or wastewater.
Scientists deployed mobile fish exposure laboratories at sites in the Shenandoah River watershed, Virginia, to understand exposure and effects on fish. This photo shows the aquariums in a laboratory where fish were exposed to stream water or wastewater.
A wastewater model was applied to the Potomac River watershed to provide (i) a means to identify streams with a high likelihood of carrying elevated effluent-derived contaminants and (ii) risk assessments to aquatic life and drinking water. The model linked effluent discharges along stream networks, accumulated wastewater, and predicted contaminant loads of municipal wastewater...
Authors
Kaycee Faunce, Larry B. Barber, Steffanie Keefe, Jeramy Roland Jasmann, Jennifer Krstolic
River waters contain complex chemical mixtures derived from natural and anthropogenic sources. Aquatic organisms are exposed to the entire chemical composition of the water, resulting in potential effects at the organismal through ecosystem level. This study applied a holistic approach to assess landscape, hydrological, chemical, and biological variables. On-site mobile laboratory...
Authors
Larry B. Barber, Kaycee Faunce, David Bertolatus, Michelle Hladik, Jeramy Roland Jasmann, Steffanie Keefe, Dana W. Kolpin, Michael T. Meyer, Jennifer Rapp, David Roth, Alan Vajda
Temporal variations of de facto wastewater reuse are relevant to public drinking water systems (PWSs) that obtain water from surface sources. Variations in wastewater discharge flows, streamflow, de facto reuse, and disinfection by-products (DBPs – trihalomethane-4 [THM4] and haloacetic acid-5 [HAA5]) over an 18-year period were examined at 11 PWSs in the Shenandoah River watershed...
Authors
Richard Weisman, Larry B. Barber, Kaycee Faunce, Jennifer Rapp, Celso Ferreira
Temporal variations of de facto wastewater reuse are relevant to public drinking water systems (PWSs) that obtain water from surface sources. Variations in wastewater discharge flows, streamflow, de facto reuse, and disinfection by-products (DBPs – trihalomethane-4 [THM4] and haloacetic acid-5 [HAA5]) over an 18-year period were examined at 11 PWSs in the Shenandoah River watershed...
Authors
Richard Weisman, Larry B. Barber, Kaycee Faunce, Jennifer Rapp, Celso Ferreira
De facto reuse is increasingly being studied among the variety of stressors that are relevant to drinking water systems that obtain their source water from surface waters. De facto reuse may influence the levels and types of precursors relevant to formation of disinfection by-products (DBPs) in surface water systems. DBPs such as trihalomethanes (THMs) and haloacetic acids (HAAs) have...
Authors
Richard Weisman, Larry B. Barber, Jennifer Rapp, Celso Ferreira
Reuse of municipal and industrial wastewater treatment plant (WWTP) effluent is an important component in augmenting global freshwater supplies. The Shenandoah River Watershed was selected to conduct on-site exposure experiments to assess endocrine disrupting characteristics of different source waters. This investigation of the Shenandoah River Watershed integrates WWTP wastewater reuse...
Authors
Larry B. Barber, Jennifer Krstolic, Chintamani Kandel, Steffanie Keefe, Jacelyn Rice, Paul Westerhoff, David Bertolatus, Alan Vajda
The Potomac Wastewater Mapper is intended to help identify streams with elevated wastewater conditions or predicted ecological risk posed by municipal effluent-derived wastewater mixtures that may require further attention by resource managers, either through targeted contaminant monitoring and sampling or wastewater treatment plant upgrades to improve contaminant removal.
Scientists refined the existing national-scale "De facto Reuse Incidence in our Nation's Consumable Supply" (DRINCS) model for the Shenandoah River watershed. The model, complemented by field measurements, provides a screening tool to understand human and wildlife exposure to toxicants and pathogens associated with the incidental reuse of treated wastewater in the watershed.
Proper management of contaminants of emerging concern in the Chesapeake Bay region requires scientific efforts to understand the risk posed to aquatic resources from the “cocktail” of multiple contaminants that is often present. This research aims to assess the occurrence, sources, environmental impacts, biological effects, and the human health impacts of toxic contaminants in rivers.
Highlighted Report: Wastewater Reuse and Predicted Ecological Risk Posed by Contaminant Mixtures in Potomac River Watershed Streams
This study applied a wastewater model to the Potomac River watershed to identify streams with a high likelihood of carrying elevated effluent-derived contaminants. Results were used in a screening-level risk assessment to evaluate the potential risks to aquatic life and drinking water posed by municipal wastewater-contaminant mixtures.
Highlighted Report: Watershed-Scale Risk to Aquatic Organisms From Complex Chemical Mixtures in the Shenandoah River
This study examined the potential and observed ecological risk posed by chemical mixtures in the Shenandoah River watershed, using a holistic approach to evaluate landscape, hydrological, chemical, and biological variables.
Major findings of efforts in the Shenandoah and Potomac River watersheds suggest that:
Wastewater reuse may be a significant source of contaminant loading to rivers
Complex chemical mixtures are widespread, resulting in both predicted and observed risks to aquatic organisms
De facto wastewater reuse (the presence of treated wastewater in drinking water sources) may negatively affect public water supplies
These findings have water resource management implications that this project will explore further in cooperation with the Chesapeake Bay Program, with the ultimate goal of reducing the impact of toxic contaminants to protect aquatic ecosystem health for generations to come.
The Chesapeake Bay and its watershed is one of America’s greatest natural treasures. However, toxic contaminants introduced into the watershed from sources such as industrial waste, municipal wastewater discharges, agricultural runoff, and stormwater runoff can harm the living resources that call the Bay home. Human health can also be affected, such as through impacts to drinking water quality or consumption of contaminated animal tissues.
One of the Chesapeake Bay Program’s goals is to reduce the impact of these contaminants on the Bay and its surrounding areas. To achieve this goal, the USGS has been leading several research efforts to: (1) gain insights into the sources, fate, and effects of toxic contaminants in the Chesapeake Bay watershed and (2) inform efforts to mitigate their harmful effects.
Scientists deployed mobile fish exposure laboratories at sites in the Shenandoah River watershed, Virginia, to understand exposure and effects on fish. This photo shows the aquariums in a laboratory where fish were exposed to stream water or wastewater.
The USGS Environmental Health Mission Area's Toxic Substances Hydrology Program has conducted several integrated studies in coordination with the Chesapeake Bay Program to better understand the occurrence and fate of toxic contaminants in surface waters, with much focus on contributions from contaminant loading from treated wastewater.
Treated wastewater is water that has been processed through physical, chemical, or biological processes to remove contaminants before being discharged into rivers and other bodies of water. This reuse of treated wastewater is an essential component of the modern water cycle that can help maintain flow of water in rivers during dry periods. Many emerging contaminants, however, are not fully removed by conventional wastewater treatment technologies. These contaminants, once discharged into the environment, may have harmful effects on aquatic life and human health.
Early studies in the Shenandoah identified a number of constituents derived from natural and anthropogenic sources in the river waters sampled, including pharmaceuticals, personal care products, pesticides, hormones, disinfection by-products, and fossil fuel combustion products. Although more than 550 individual constituents were found, these represent only a small fraction of the potential contaminants present in the water. Since then, holistic integrated approaches have been developed to assess the perceived versus actual risk posed to aquatic organisms and human health from exposure to these complex chemical mixtures derived from water reuse and other sources in the Shenandoah as well as the broader Potomac River watershed, of which the Shenandoah is a subbasin.
A fundamental challenge in conducting integrated transdisciplinary research is linking continental-scale issues to molecular-scale processes. This requires the ability to upscale or downscale to address a particular study need while maintaining the integration of landscape, hydrological, chemical, and biological processes.
The approaches used by this project to address these challenges include:
Documenting the effects of streamflow and landscape variables on contaminant concentrations and determining the relations to the cumulative loadings from wastewater treatment plants. This involved developing an accumulated wastewater ratio model for the Shenandoah River watershed that: (1) accounts for upstream contributions of all municipal and industrial effluent discharges and (2) calculates the percentage of wastewater for each stream in the watershed.
Determining the spatial and temporal concentration and composition patterns of 273 constituents detected in water sampled collected in the Shenandoah River watershed between 2014 and 2016.
Conducting on-site mobile laboratory exposure studies to evaluate the relations between fish endocrine disruption and endocrine-disrupting chemicals in both streams and municipal wastewater effluent. These experiments applied and refined protocols developed at wastewater treatment plants and impacted streams by other studies (Barber et al., 2007; Vajda et al., 2011; Barber et al., 2012).
Focusing on effects of complex chemical mixture exposure on physiological, molecular, and multi-omics pathways for the model organism fathead minnow.
Associated report currently in review: Bertolatus, D.W., Barber, L.B., Martyniuk, C.J., Zhen, H, Collette, T.W., Ekman, D.R., Jastrow, A., Rapp, J.L., and Vajda, A.M., 2022 – in review, Watershed-scale integrated ecotoxicological assessment: Multi-omic responses of fathead minnows exposed to complex chemical mixtures. To be submitted to Science of the Total Environment.
Focusing on effects of de facto wastewater reuse on disinfection byproduct formation in public water supply systems.
Abbott, B.W., Bishop, K., Zarnetske, J.P., Minaudo, C., Chapin, F.S., III, Krause, S., Hannah, D.M., Conner, L., Ellison, D., Godsey, S.E., Plont, S., Marcais, J., Kolbe, T., Huebner, A., Frei, R.J., Hampton, T., Gu, S., Buhman, M., Sayedi, S.S., Ursache, O., Chapin, M., Henderson, K.D., and Pinay, G., 2019, Human domination of the global water cycle absent from depictions and perceptions. Nature Geoscience, v. 12, p. 533-540.
Barber, L.B., Lee, K.E., Swackhamer, D.L., and Schoenfuss, H.L., 2007, Reproductive responses of male fathead minnows exposed to wastewater treatment plant effluent, effluent treated with XAD8 resin, and an environmentally relevant mixture of alkylphenol compounds. Aquatic Toxicology, v. 82, p. 36-46.
Barber, L.B., Vajda, A.M., Douville, C., Norris, D.O., and Writer, J.H., 2012, Fish endocrine disruption responses to a major wastewater treatment facility upgrade. Environmental Science and Technology, v. 46, p. 2121-2131.
Bergman, A., Heindel, J.J., Jobling, S., Kidd, K.A., and Zoeller, R.T., 2013, State of the Science of Endocrine Disrupting Chemicals – 2012. World Health Organization, Geneva, Switzerland.
Blazer, V.S., Iwanowicz, L.R., Henderson, H., Mazik, P.M., Jenkins, J.A., Alvarez, D.A., and Young, J.A., 2012, Reproductive endocrine disruption in smallmouth bass (Micropterus dolomieu) in the Potomac River basin: spatial and temporal comparisons of biological effects. Environmental Monitoring and Assessment, v. 184, p. 4309-4334.
Bradley, P.M., Journey, C.A., Romanok, K.M., Barber, L.B., Buxton, H.T., Foreman, W.T., Furlong, E.T., Glassmeyer, S.T., Hladik, M.L., Iwanowicz, L.R., Jones, D.K., Kolpin, D.W., Kuivila, K.M., Loftin, K.A., Mills, M.A., Meyer, M.T., Orlando, J.L., Reilly, T.J., Smalling, K.L., and Villeneuve, D.L., 2017, Expanded target-chemical analysis reveals extensive mixed-organic-contaminant exposure in USA streams. Environmental Science and Technology, v. 51, p. 4792-4802.
Dieter, C.A., Maupin, M.A., Caldwell, R.R., Harris, M.A., Ivahnenko, T.I., Lovelace, J.K., Barber, N.L., and Linsey, K.S., 2018, Estimated use of water in the United States in 2015. U.S. Geological Survey Circ. 1441: U.S. Geological Survey; Washington, DC. Accessed March 2020 at [https://doi.org/10.3133/cir1441].
Gordon, S., Jones, D.K., Blazer, V.S., Iwanowicz, L., Williams, B., and Smalling, K., 2021, Modeling estrogenic activity in streams throughout the Potomac and Chesapeake Bay watersheds. Environmental Monitoring and Assessment, v. 193, Article number 105.
Kolpin, D.W., Furlong, E.T., Meyer, M.T., Thurman, E.M., Zaugg, S.D., Barber, L.B., and Buxton, H.T., 2002, Pharmaceuticals, hormones, and other organic wastewater contaminants in U.S. streams, 1999-2000 - A national reconnaissance. Environmental Science and Technology, v. 36, p. 1202-1211.
Kolpin, D.W., Blazer, V.S., Gray, J.L., Focazio, M.J., Young, J.A., Alvarez, D.A., Iwanowicz, L.R., Foreman, W.T., Furlong, E.T., Speiran, G.K., Zaugg, S.D., Hubbard, L.E., Meyer, M.T., Sandstrom, M.W., and Barber, L.B., 2013, Chemical contaminants in water and sediment near fish nesting sites in the Potomac River Basin: Determining potential exposures to smallmouth bass (Micropterus dolomieu). Science of the Total Environment, v. 443, p. 700-716.
Masoner, J.R., Kolpin, D.W., Cozzarelli, I.M., Barber, L.B., Burden, D.S., Foreman, W.T., Forshay, K.J., Furlong, E.T., Groves, J.F., Hladik, M.L., Hopton, M.E., Jaeschke, J.B., Keefe, S.H., Krabbenhoft, D.P., Lowrance, R., Romanok, K.M., Rus, D.L., Selbig, W.R., Williams, B.H., and Bradley, P.M., 2019, Urban stormwater: An overlooked pathway of extensive mixed contaminants to surface and groundwaters in the United States. Environmental Science and Technology, v. 53, p. 10070-10081.
National Research Council, 2012, Water Reuse: Potential for Expanding the Nation’s Water Supply through Reuse of Municipal Wastewater. National Academies Press, Washington, DC.
United Nations World Water Assessment Programme, 2017, Wastewater – The Untapped Resource. The United Nations World Water Development Report 2017, UNESCO, Paris, France.
U.S. Environmental Protection Agency, 2020, National water reuse action plan: Improving the security, sustainability, and resilience of our nation’s water resources. U.S. Environmental Protection Agency Collaborative Implementation, Version 1: U.S. Environmental Protection Agency; Washington, DC, 40 p. plus Appendices.
Vajda, A.M., Barber, L.B., Gray, J.L., Lopez, E.M., Bolden, A.M., Schoenfuss, H.L., and Norris, D.O., 2011, Demasculinization of male fish by wastewater treatment plant effluent. Aquatic Toxicology, v. 103, p. 213-221.
Wastewater is used water. It includes substances such as human waste, food scraps, oils, soaps and chemicals. In homes, this includes water from sinks, showers, bathtubs, toilets, washing machines and dishwashers. Businesses and industries also contribute their share of used water that must be cleaned.
Wastewater is used water. It includes substances such as human waste, food scraps, oils, soaps and chemicals. In homes, this includes water from sinks, showers, bathtubs, toilets, washing machines and dishwashers. Businesses and industries also contribute their share of used water that must be cleaned.
Issue: Municipal and industrial wastewater effluent is an important source of water for streams and rivers, especially during periods of low flow. The reuse of wastewater effluent may become even more important if climate change exacerbates low streamflow and drought conditions. However, wastewater effluent often contains chemicals that, when chronically present, can affect the health of aquatic...
Wastewater reuse may be detrimental to smallmouth bass abundance in the Shenandoah River Watershed
Issue: Municipal and industrial wastewater effluent is an important source of water for streams and rivers, especially during periods of low flow. The reuse of wastewater effluent may become even more important if climate change exacerbates low streamflow and drought conditions. However, wastewater effluent often contains chemicals that, when chronically present, can affect the health of aquatic...
A key component to assessing the contaminant exposure pathways in streams and rivers of the Chesapeake Bay is using GIS-based landscape analysis to identify sources of endocrine disrupting chemicals. Municipal and industrial wastewater treatment plant (WWTP) discharges are potentially major sources of EDCs to streams, and therefore understanding the de facto wastewater reuse (represented as...
GIS-based landscape analysis to identify sources of endocrine disrupting chemicals
A key component to assessing the contaminant exposure pathways in streams and rivers of the Chesapeake Bay is using GIS-based landscape analysis to identify sources of endocrine disrupting chemicals. Municipal and industrial wastewater treatment plant (WWTP) discharges are potentially major sources of EDCs to streams, and therefore understanding the de facto wastewater reuse (represented as...
De facto wastewater reuse from Waste Water Treatment Facilities (WWTF) has the potential to be a significant contributor of Endocrine Disrupting Chemicals. An ArcGIS model of WWTFs, NHDPlus Version 2 stream networks (USGS and EPA 2012), and gage stations across the Shenandoah River watershed was created to calculate accumulated wastewater ratio. Virginia Pollutant Discharge Elimination...
This data release presents chemical and biological results from investigations of water quality, fish endocrine disruption, and emergent insects in the Shenandoah River Watershed (Virginia and West Virginia, USA) conducted during 2014, 2015, and 2016. Multiple sampling campaigns were conducted at sites located throughout the Shenandoah River Watershed (Table 1). The complex inorganic and...
Mobile Fish Exposure Laboratory on Site in the Shenandoah River Waters
One of the mobile fish exposure laboratories that were deployed at sites in the Shenandoah River watershed, Virginia. Fish were housed in the mobile laboratories and exposed to stream water or wastewater during the study to understand exposure and effects on fish.
One of the mobile fish exposure laboratories that were deployed at sites in the Shenandoah River watershed, Virginia. Fish were housed in the mobile laboratories and exposed to stream water or wastewater during the study to understand exposure and effects on fish.
Aquariums Inside a Mobile Fish Exposure Laboratory
Scientists deployed mobile fish exposure laboratories at sites in the Shenandoah River watershed, Virginia, to understand exposure and effects on fish. This photo shows the aquariums in a laboratory where fish were exposed to stream water or wastewater.
Scientists deployed mobile fish exposure laboratories at sites in the Shenandoah River watershed, Virginia, to understand exposure and effects on fish. This photo shows the aquariums in a laboratory where fish were exposed to stream water or wastewater.
A wastewater model was applied to the Potomac River watershed to provide (i) a means to identify streams with a high likelihood of carrying elevated effluent-derived contaminants and (ii) risk assessments to aquatic life and drinking water. The model linked effluent discharges along stream networks, accumulated wastewater, and predicted contaminant loads of municipal wastewater...
Authors
Kaycee Faunce, Larry B. Barber, Steffanie Keefe, Jeramy Roland Jasmann, Jennifer Krstolic
River waters contain complex chemical mixtures derived from natural and anthropogenic sources. Aquatic organisms are exposed to the entire chemical composition of the water, resulting in potential effects at the organismal through ecosystem level. This study applied a holistic approach to assess landscape, hydrological, chemical, and biological variables. On-site mobile laboratory...
Authors
Larry B. Barber, Kaycee Faunce, David Bertolatus, Michelle Hladik, Jeramy Roland Jasmann, Steffanie Keefe, Dana W. Kolpin, Michael T. Meyer, Jennifer Rapp, David Roth, Alan Vajda
Temporal variations of de facto wastewater reuse are relevant to public drinking water systems (PWSs) that obtain water from surface sources. Variations in wastewater discharge flows, streamflow, de facto reuse, and disinfection by-products (DBPs – trihalomethane-4 [THM4] and haloacetic acid-5 [HAA5]) over an 18-year period were examined at 11 PWSs in the Shenandoah River watershed...
Authors
Richard Weisman, Larry B. Barber, Kaycee Faunce, Jennifer Rapp, Celso Ferreira
Temporal variations of de facto wastewater reuse are relevant to public drinking water systems (PWSs) that obtain water from surface sources. Variations in wastewater discharge flows, streamflow, de facto reuse, and disinfection by-products (DBPs – trihalomethane-4 [THM4] and haloacetic acid-5 [HAA5]) over an 18-year period were examined at 11 PWSs in the Shenandoah River watershed...
Authors
Richard Weisman, Larry B. Barber, Kaycee Faunce, Jennifer Rapp, Celso Ferreira
De facto reuse is increasingly being studied among the variety of stressors that are relevant to drinking water systems that obtain their source water from surface waters. De facto reuse may influence the levels and types of precursors relevant to formation of disinfection by-products (DBPs) in surface water systems. DBPs such as trihalomethanes (THMs) and haloacetic acids (HAAs) have...
Authors
Richard Weisman, Larry B. Barber, Jennifer Rapp, Celso Ferreira
Reuse of municipal and industrial wastewater treatment plant (WWTP) effluent is an important component in augmenting global freshwater supplies. The Shenandoah River Watershed was selected to conduct on-site exposure experiments to assess endocrine disrupting characteristics of different source waters. This investigation of the Shenandoah River Watershed integrates WWTP wastewater reuse...
Authors
Larry B. Barber, Jennifer Krstolic, Chintamani Kandel, Steffanie Keefe, Jacelyn Rice, Paul Westerhoff, David Bertolatus, Alan Vajda
The Potomac Wastewater Mapper is intended to help identify streams with elevated wastewater conditions or predicted ecological risk posed by municipal effluent-derived wastewater mixtures that may require further attention by resource managers, either through targeted contaminant monitoring and sampling or wastewater treatment plant upgrades to improve contaminant removal.
Scientists refined the existing national-scale "De facto Reuse Incidence in our Nation's Consumable Supply" (DRINCS) model for the Shenandoah River watershed. The model, complemented by field measurements, provides a screening tool to understand human and wildlife exposure to toxicants and pathogens associated with the incidental reuse of treated wastewater in the watershed.