Genetics/Genomics

New terrestrial environmental DNA (eDNA) sampling techniques are sensitive tools for early detection of the highly invasive brown treesnakes (BTS). In a new collaborative research effort involving four USGS science centers, scientists have initiated the development of an innovative point-of-use (POU) assay for BTS. POU assays are tests that can be run in the field with minimal equipment and no...

A multi-agency effort assesses the health of manatees and provides baseline information on their health, reproductive status, and nutritional condition.

The Asian tiger shrimp began appearing in commercial shrimp catches in 2006. They grow larger than native shrimp and are known to be fierce predators - so shrimpers and managers are concerned about the potential effects this species might have.

Flow cytometry is a technique for rapidly analyzing large numbers of animal cells using light-scattering, fluorescence, and absorbance measurements.

White-nose syndrome has killed more than five million bats since its discovery in 2006. USGS is developing a non-invasive and user-friendly CRISPR white-nose syndrome biosensor to allow for quick detection of the pathogen that causes the disease.

Researchers will develop and optimize an eDNA assay to delineate the current distribution of P. inflatus. The assay will then be used to provide up-to-date distributional information and detection rates for P. inflatus in the Pearl River basin.

USGS researchers are working with partners to assess the health and foraging behavior of Florida manatees in the northern Indian River Lagoon and Banana River, areas that have experienced declining seagrasses due to an extended phytoplankton bloom.

Freshwater mussels of the family Unionidae, also known as naiads, pearly mussels, freshwater clams, or unionids, are a diverse group of bivalve mollusks that are distributed on every continent except Antarctica. Approximately 300 species are known from the United States, with most of this diversity residing in rivers of the Southeast where many endemic taxa have evolved.

Differentiating diploids from triploids at the earliest life stage possible allows for a more efficient use of resources including production time and rearing space. Thus, a reliable flow cytometric (FCM) method has been developed to discriminate triploids from diploids at the larval stage. In order to help simplify the process of differentiating triploids from diploids, we propose a simple...

Nuclear microsatellite markers have been developed and implemented on ~2,000 West Indian manatees. These markers provide individual genetic fingerprints for mark-recapture studies, population structure information for the conservation of unique or isolated populations, and pedigree and relatedness information for addressing inbreeding and breeding structure patterns.

Detecting invasive species at low densities or prior to population establishment is critical for successful control and eradication. For example, Burmese pythons occupy thousands of square kilometers of mostly inaccessible habitats.

The invasive grass carp and black carp are artificially spawned to produce triploids, which means they have three sets of chromosomes and are sterile. WARC scientists invented an early ploidy prediction process for produced fry, and a post-mortem assessment method for carp caught either in the wild or sold and hauled live to other states.