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Find out more about Biological Threat and Invasive Species Research through our publications.

Filter Total Items: 305

A ribonuclease protection assay can distinguish spring viremia of carp virus from pike fry rhabdovirus

Thirteen rhabdovirus isolates from 10 teleost fish species as well as reference strains of spring viraemia of carp virus (SVCV) and pike fry rhabdovirus (PFRV) cross-reacted in an indirect immunofluorescence assay and were thus indistinguishable by this method. A ribonuclease protection assay (RPA) using a super(32)P-labeled RNA probe made from a cloned copy of the full length SVCV glycoprotein (G
Authors
W. Ahne, Gael Kurath, J. R. Winton

Wildlife disease and conservation in Hawaii: pathogenicity of avian malaria (Plasmodium relictum) in experimentally infected Iiwi (Vestiaria coccinea)

Native Hawaiian forest birds are facing a major extinction crisis with more than 75% of species recorded in historical times either extinct or endangered. Reasons for this catastrophe include habitat destruction, competition with non-native species, and introduction of predators and avian diseases. We tested susceptibility of Iiwi (Vestiaria coccinea), a declining native species, and Nutmeg Mannik
Authors
C. T. Atkinson, K.L. Woods, Robert J. Dusek, L.S. Sileo, W.M. Iko

Development of an antimycin-impregnated bait for controlling common carp

The common carp Cyprinus carpio is a major problem for fisheries and wildlife managers because its feeding behavior causes degradation of valuable fish and waterfowl habitat. This study was designed to evaluate the effectiveness of an antimycin-impregnated bait for control of common carp. The toxic bait contained fish meal, a binder, antimycin, and water. The ingredients were mixed together and
Authors
J.J. Rach, J.A. Luoma, L. L. Marking

Enzyme-linked immunosorbent assay for a soluble antigen of Renibacterium salmoninarum, the causative agent for salmonid bacterial kidney disease

A double-antibody enzyme-linked immunosorbent assay (ELISA) for detection of a soluble fraction of Renibacterium salmoninarum was developed from components extracted from the supernatant of an R. salmoninarum broth culture. The Costar® Serocluster™ EIA microplate gave the highest absorbance and signal-to-noise ratios among seven types tested. Including Tween 80 in the wash buffer resulted in highe
Authors
R.J. Pascho, D. Mulcahy

Demographic patterns of the shrub Ceanothus megacarpus in an old stand of chaparral in the Santa Monica Mountains

Wildfires have had a major influence on the structural and functional adaptations that have evolved in Mediterranean-type ecosystems. Some chaparral shrubs sprout after fires while others produce serotinous cones or seeds refractory to germination until they are cued by a fire. Ceanothus megacarpus is a sclerophylous shrub commonly found in California in either pure of mixed stands which does no
Authors
T. M. Montygierd-Loyba, J. E. Keeley

Female homogamety in grass carp (Ctenopharyngodon idella) determined by gynogenesis

Gynogenesis occurred in eggs of grass carp (Ctenopharyngodon idella) treated with X-irradiated milt from goldfish (Carassius auratus). Gynogenetic offspring were females, which indicates functional female homogamety in grass carp. Five of these gynogenetic fish were used as an egg source for a second generation of artificially gynogenetic fish. The percentage yield in this second generation was ab
Authors
Jon G. Stanley

Oral immunization of rainbow trout (Salmo gairdneri) against an etiologic agent of "redmouth disease"

Rainbow trout were fed a pelleted diet containing killed cells of the etiologic agent of a bacterial disease, redmouth. These fish in addition to appropriate controls were subsequently challenged with virulent homologous organisms. Ninety per cent of the redmouth immunized fish survived the basic challenge using virulent organisms in contrast to 20% survival for the controls. Multiple challenge do

Downstream movement of lampreys and fish in the Carp Lake River, Michigan

An inclined-screen trap was installed on the Carp River, Emmett County, Michigan, in the spring of 1948 and has been in almost continuous operation since that time. The major goal of this project--a precise determination of the length of the larval life of sea lamprey--was not attained because of the contamination of the stream above the dam with spawning lampreys. The lampreys and other fishes co
Authors
Vernon C. Applegate

A "virus" disease of chinook salmon

Epizootics among chinook salmon fingerlings at the Coleman National Fish Hatchery have occurred periodically since 1941. A virus or virus-like filterable agent has been demonstrated to be the causative agent of this disease.
Authors
A. J. Ross, R.R. Rucker