Cathy Richter, PhD
Dr. Cathy Richter is a Research Molecular Biologist at the Columbia Environmental Research Center.
Science and Products
Filter Total Items: 25
Data on the detection of Epioblasma capsaeformis using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Clinch River, Tennessee (2019 to 2021) Data on the detection of Epioblasma capsaeformis using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Clinch River, Tennessee (2019 to 2021)
Data describe a designed environmental DNA (eDNA) survey for the detection of oyster mussel (Epioblasma capsaeformis) from field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater mussel species tissue samples that were used to test specificity of the assay; and field collected water samples that...
Data on the detection of Cumberlandia monodonta using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Big Piney River, Missouri 2020 – 2022 (ver. 2.0, August 2024) Data on the detection of Cumberlandia monodonta using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Big Piney River, Missouri 2020 – 2022 (ver. 2.0, August 2024)
Data describe a designed environmental DNA (eDNA) survey for the detection of Spectaclecase (Cumberlandia monodonta, also referred to as Margaritifera monodonta) from field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater mussel species tissue samples that were used to test specificity of the assay...
Degradation of Cumberlandia monodonta environmental DNA over time in laboratory conditions using the C.monCOI assay Degradation of Cumberlandia monodonta environmental DNA over time in laboratory conditions using the C.monCOI assay
Data describe a designed environmental DNA (eDNA) experiment for the estimation of eDNA degradation rates for the spectaclecase (Cumberlandia monodonta) from water samples collected in the laboratory. Parameters described include laboratory collected water samples that were tested to estimate concentration of spectaclecase DNA and associated quality assurance data. Samples were collected...
Thiaminase activity measurements in whole zebrafish Thiaminase activity measurements in whole zebrafish
Data describe results of a set of laboratory experiments using a previously described quantitative thiaminase I activity assay to test the thiaminase activity of whole zebrafish homogenates. Parameters described include the thiaminase activity data, dilution factors, incubation time, and tissue weight assayed. Experiments were conducted at the USGS Columbia Environmental Research Center...
Water chemistry and fish metrics data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture Water chemistry and fish metrics data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture
Physiological endpoints observed in adult largemouth bass in response to an EE2 (17 alpha-ethinylestradiol) or mixture of estrone and atrazine exposure under laboratory pond conditions. Also included are water quality and chemical concentration data. Adult LMB were exposed in outdoor pond mesocosms from post-spawning to early gonad recrudescence to either EE2 (2.4 nanograms per liter)...
Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions.
Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Also included are water quality and chemical concentration data.
Polyacrylamide gel electrophoresis results with thiaminase activity stain of recombinant putative thiaminases expressed in E. coli Polyacrylamide gel electrophoresis results with thiaminase activity stain of recombinant putative thiaminases expressed in E. coli
Data describe results of a set of laboratory experiments using polyacrylamide gel electrophoresis with a thiaminase activity stain to test the solubility and thiaminase activity of recombinant putative thiaminases expressed in E. coli. Parameters described include the presence and relative mobility of thiaminases, and the presence and relative mobility of recombinant proteins...
Longnose darter (Percina nasuta) eDNA survey results from the St. Francis River, Missouri 2018 Longnose darter (Percina nasuta) eDNA survey results from the St. Francis River, Missouri 2018
Data describe a designed environmental DNA (eDNA) survey for the detection of longnose darter (Percina nasuta) in field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater fish species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to...
Metabarcoding data from freshwater mussel eDNA collected in Clinch River, Virginia 2017 Metabarcoding data from freshwater mussel eDNA collected in Clinch River, Virginia 2017
Data from metabarcoding assays to detect a suite of mussel species using mitochondrial DNA regions of the cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit (ND1) genes sequences.
Mucket eDNA detection in Wallen's Bend, Clinch river, Tennessee, September 2019 Mucket eDNA detection in Wallen's Bend, Clinch river, Tennessee, September 2019
The data describe the technical performance of a designed qPCR assay for the detection of mucket (Actinonaias ligamentina) eDNA from field collected water samples. Parameters described include the sequences of the primers and probes used; the limit of detection and limit of quantification for the assay; a list of freshwater mussels species that were used to test specificity of the assay...
Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17a-ethinylestradiol) Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17a-ethinylestradiol)
Largemouth base histological development and transcriptomic changes in gonad tissue after early life stage exposure to Atrazine (1-Chloro-3-ethylamino-5-isopropylamino-2,4,6-triazine) or the model estrogen 17a-ethinylestradiol.
Asian carp eDNA and egg morphology data collected from Truman Reservoir tributaries, Missouri, USA, 2014 Asian carp eDNA and egg morphology data collected from Truman Reservoir tributaries, Missouri, USA, 2014
Ichthyoplankton tows samples collected from Truman reservoir tributaries evaluated for eDNA. Data also includes hydrologic and water quality parameters and morphology of grass carp eggs.
Filter Total Items: 49
Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio
Thiamine (vitamin B1) metabolism is an important driver of human and animal health and ecological functioning. Some organisms, including species of ferns, mollusks, and fish, contain thiamine-degrading enzymes known as thiaminases, and consumption of these organisms can lead to thiamine deficiency in the consumer. Consumption of fish containing thiaminase has led to elevated mortality...
Authors
Cathy Richter, Allison Evans, Scott A. Heppell, James L. Zajicek, Donald Tillitt
Grass carp reproduction in small tributaries of Truman Reservoir, Missouri: Implications for establishment in novel habitats Grass carp reproduction in small tributaries of Truman Reservoir, Missouri: Implications for establishment in novel habitats
Substantial work has been conducted to estimate the river length required for recruitment of invasive Grass Carp Ctenopharyngodon idella and bigheaded carps (Bighead Carp Hypophthalmichthys nobilis and Silver Carp H. molitrix); however, the distance upstream and size of stream required for spawning remain unclear. Adult Grass Carp are regularly captured in Harry S. Truman Reservoir...
Authors
Cari-Ann Hayer, Michael Bayless, Cathy Richter, Amy George, Duane Chapman
Declines in reproductive condition of male largemouth bass (Micropterus salmoides) following seasonal exposure to estrogenic endocrine-disrupting compounds Declines in reproductive condition of male largemouth bass (Micropterus salmoides) following seasonal exposure to estrogenic endocrine-disrupting compounds
Reproductive abnormalities, that could lead to possible effects at the population level, have been observed in wild fish throughout the United States, with high prevalence in largemouth bass (LMB; Micropterus salmoides) and smallmouth bass (Micropterus dolomieu). Estrone (E1) and atrazine (ATR) are common environmental contaminants often associated with agricultural land use. 17alpha...
Authors
Jessica Leet, Cathy Richter, Robert Gale, Donald Tillitt, Jill Jenkins
Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection
Disease outbreaks, skin lesions, mortality events, and reproductive abnormalities have been observed in wild populations of centrarchids. The presence of estrogenic endocrine disrupting compounds (EEDCs) has been implicated as a potential causal factor for these effects. The effects of prior EEDC exposure on immune response were examined in juvenile largemouth bass (Micropterus salmoides...
Authors
Jessica Leet, Justin Greer, Cathy Richter, Luke R. Iwanowicz, Edward Spinard, Jacquelyn McDonald, Carla Conway, Robert Gale, Donald Tillitt, John Hansen
Using structured decision making to evaluate potential management responses to detection of dreissenid mussel (Dreissena spp.) environmental DNA Using structured decision making to evaluate potential management responses to detection of dreissenid mussel (Dreissena spp.) environmental DNA
Environmental (e)DNA tools are sensitive and cost-effective for early detection of invasive species. However, the uncertainty associated with the interpretation of positive eDNA detections makes it challenging to determine appropriate natural resource management responses. Multiple sources of error can give rise to positive detections of eDNA in a sample when individuals of that species...
Authors
Adam Sepulveda, David Smith, Katherine M. O'Donnell, Nathan Owens, Brittany White, Cathy Richter, Christopher Merkes, Skylar Wolf, Mike Rau, Matthew Neilson, Wesley M. Daniel, Christine Dumoulin, Margaret Hunter
A comparison of eDNA and visual survey methods for detection of longnose darter Percina nasuta in Missouri A comparison of eDNA and visual survey methods for detection of longnose darter Percina nasuta in Missouri
The longnose darter Percina nasuta is a rare and cryptic fish that recently disappeared from much of its historic range. We developed and used an environmental DNA (eDNA) assay for longnose darter paired with visual surveys to better determine the species’ range and compare detection probability between sampling approaches in an occupancy modeling framework. We detected longnose darter...
Authors
Jacob Westhoff, Leah Berkman, Katy Klymus, Nathan Thompson, Cathy Richter
Reproduction Reproduction
Lake charr Salvelinus namaycush are typically fall spawners although one ecotype has populations that spawn during spring and fall (siscowets in Lake Superior). Lake charr are iteroparous (reproduce more than once in a lifetime) with group-synchronous ovarian development and typically spawn once per year. However, lake charr may not reproduce every year, a phenomenon known as skipped...
Authors
Frederick W. Goetz, J. Ellen Marsden, Catherine Richter, Donald Tillitt, Shawn Sitar, Stephen Riley, Charles C. Krueger
Metabarcoding assays for the detection of freshwater mussels (Unionida) with environmental DNA Metabarcoding assays for the detection of freshwater mussels (Unionida) with environmental DNA
Freshwater mussels of the order Unionida are a widely distributed taxon that are important in maintaining freshwater ecosystems and are also highly imperiled throughout the world. Monitoring of mussel populations with environmental DNA (eDNA) is an attractive alternative to traditional methods because it is noninvasive and requires less labor and taxonomic knowledge from field personnel...
Authors
Katy Klymus, Catherine Richter, Nathan Thompson, Jo Hinck, Jess Jones
Development and testing of species-specific quantitative PCR assays for environmental DNA applications Development and testing of species-specific quantitative PCR assays for environmental DNA applications
New, non-invasive methods for detecting and monitoring species presence are being developed to aid in fisheries and wildlife conservation management. The use of environmental DNA (eDNA) samples for detecting macrobiota is one such group of methods that is rapidly becoming popular and being implemented in national management programs. Here we focus on the development of species-specific...
Authors
Katy Klymus, Dannise Ruiz-Ramos, Nathan Thompson, Catherine Richter
Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17α-ethinylestradiol) Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17α-ethinylestradiol)
Endocrine disrupting contaminants are of continuing concern for potentially contributing to reproductive dysfunction in largemouth and smallmouth bass in the Chesapeake Bay watershed (CBW) and elsewhere. Exposures to atrazine (ATR) have been hypothesized to have estrogenic effects on vertebrate endocrine systems. The incidence of intersex in male smallmouth bass from some regions of CBW...
Authors
Jessica Leet, Catherine Richter, Robert Cornman, Jason P. Berninger, Ramji K. Bhandari, Diane K. Nicks, James L. Zajicek, Vicki S. Blazer, Donald Tillitt
Use of environmental DNA to detect grass carp spawning events Use of environmental DNA to detect grass carp spawning events
The timing and location of spawning events are important data for managers seeking to control invasive grass carp populations. Ichthyoplankton tows for grass carp eggs and larvae can be used to detect spawning events; however, these samples can be highly debris-laden, and are expensive and laborious to process. An alternative method, environmental DNA (eDNA) technology, has proven...
Authors
Cari-Ann Hayer, Michael Bayless, Amy George, Nathan Thompson, Catherine Richter, Duane Chapman
Reporting the limits of detection and quantification for environmental DNA assays Reporting the limits of detection and quantification for environmental DNA assays
BackgroundEnvironmental DNA (eDNA) analysis is increasingly being used to detect the presence and relative abundance of rare species, especially invasive or imperiled aquatic species. The rapid progress in the eDNA field has resulted in numerous studies impacting conservation and management actions. However, standardization of eDNA methods and reporting across the field is yet to be...
Authors
Katy Klymus, Christopher Merkes, Michael Allison, Caren Goldberg, Caren Helbing, Margaret Hunter, Craig Jackson, Richard Lance, Anna M. Mangan, Emy Monroe, Antoinette Piaggio, Joel Stokdyk, Chris Wilson, Catherine Richter
Non-USGS Publications**
Taylor, J.A., Richter, C.A., Suzuki, A., Watanabe, H., Iguchi, T., Coser, K.R., Shioda, T., and vom Saal, F.S., 2012, Dose-related estrogen effects on gene expression in fetal mouse prostate mesenchymal cells: PLOS ONE, v. 7, no. 10, p. e48311
Taylor, J.A., Richter, C.A., Ruhlen, R.L., and vom Saal, F.S., 2011, Estrogenic environmental chemicals and drugs: Mechanisms for effects on the developing male urogenital system: The Journal of Steroid Biochemistry and Molecular Biology, v. 127, no. 1, p. 83-95
Richter, C.A., Taylor, J.A., Ruhlen, R.L., Welshons, W.V., and vom Saal, F.S., 2007, Estradiol and Bisphenol A stimulate androgen receptor and estrogen receptor gene expression in fetal mouse prostate mesenchyme cells: Environmental Health Perspectives, v. 115, no. 6, p. 902-908
vom Saal, F.S., Richter, C.A., Mao, J., and Welshons, W.V., 2005, Commercial animal feed: Variability in estrogenic activity and effects on body weight in mice: Birth Defects Research Part A: Clinical and Molecular Teratology, v. 73, no. 7, p. 474-475
Timms, B.G., Howdeshell, K.L., Barton, L., Bradley, S., Richter, C.A., and vom Saal, F.S., 2005, Estrogenic chemicals in plastic and oral contraceptives disrupt development of the fetal mouse prostate and urethra: Proceedings of the National Academy of Sciences of the United States of America, v. 102, no. 19, p. 7014-7019
Richter, C., Timms, B., and vom Saal, F., 2005, Prostate development: mechanisms for opposite effects of low and high doses of estrogenic chemicals, in Naz, R., ed., Endocrine Disruptors: Effects on Male and Female Reproductive Systems: New York, CRC Press, p. 379-410.
vom Saal, F.S., Richter, C.A., Ruhlen, R.R., Nagel, S.C., Timms, B.G., and Welshons, W.V., 2005, The importance of appropriate controls, animal feed, and animal models in interpreting results from low-dose studies of bisphenol A: Birth Defects Research Part A: Clinical and Molecular Teratology, v. 73, no. 3, p. 140-145
vom Saal, F.S., Richter, C.A., Ruhlen, R.R., Nagel, S.C., and Welshons, W.V., 2004, Disruption of laboratory experiments due to leaching of Bisphenol A from polycarbonate cages and bottles and uncontrolled variability in components of animal feed, in National Research Council, ed., The Development of Science-based Guidelines for Laboratory Animal Care: Proceedings of the November 2003 International Workshop: The National Academies Press, p. 65-69.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Science and Products
Filter Total Items: 25
Data on the detection of Epioblasma capsaeformis using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Clinch River, Tennessee (2019 to 2021) Data on the detection of Epioblasma capsaeformis using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Clinch River, Tennessee (2019 to 2021)
Data describe a designed environmental DNA (eDNA) survey for the detection of oyster mussel (Epioblasma capsaeformis) from field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater mussel species tissue samples that were used to test specificity of the assay; and field collected water samples that...
Data on the detection of Cumberlandia monodonta using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Big Piney River, Missouri 2020 – 2022 (ver. 2.0, August 2024) Data on the detection of Cumberlandia monodonta using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Big Piney River, Missouri 2020 – 2022 (ver. 2.0, August 2024)
Data describe a designed environmental DNA (eDNA) survey for the detection of Spectaclecase (Cumberlandia monodonta, also referred to as Margaritifera monodonta) from field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater mussel species tissue samples that were used to test specificity of the assay...
Degradation of Cumberlandia monodonta environmental DNA over time in laboratory conditions using the C.monCOI assay Degradation of Cumberlandia monodonta environmental DNA over time in laboratory conditions using the C.monCOI assay
Data describe a designed environmental DNA (eDNA) experiment for the estimation of eDNA degradation rates for the spectaclecase (Cumberlandia monodonta) from water samples collected in the laboratory. Parameters described include laboratory collected water samples that were tested to estimate concentration of spectaclecase DNA and associated quality assurance data. Samples were collected...
Thiaminase activity measurements in whole zebrafish Thiaminase activity measurements in whole zebrafish
Data describe results of a set of laboratory experiments using a previously described quantitative thiaminase I activity assay to test the thiaminase activity of whole zebrafish homogenates. Parameters described include the thiaminase activity data, dilution factors, incubation time, and tissue weight assayed. Experiments were conducted at the USGS Columbia Environmental Research Center...
Water chemistry and fish metrics data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture Water chemistry and fish metrics data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture
Physiological endpoints observed in adult largemouth bass in response to an EE2 (17 alpha-ethinylestradiol) or mixture of estrone and atrazine exposure under laboratory pond conditions. Also included are water quality and chemical concentration data. Adult LMB were exposed in outdoor pond mesocosms from post-spawning to early gonad recrudescence to either EE2 (2.4 nanograms per liter)...
Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions.
Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Also included are water quality and chemical concentration data.
Polyacrylamide gel electrophoresis results with thiaminase activity stain of recombinant putative thiaminases expressed in E. coli Polyacrylamide gel electrophoresis results with thiaminase activity stain of recombinant putative thiaminases expressed in E. coli
Data describe results of a set of laboratory experiments using polyacrylamide gel electrophoresis with a thiaminase activity stain to test the solubility and thiaminase activity of recombinant putative thiaminases expressed in E. coli. Parameters described include the presence and relative mobility of thiaminases, and the presence and relative mobility of recombinant proteins...
Longnose darter (Percina nasuta) eDNA survey results from the St. Francis River, Missouri 2018 Longnose darter (Percina nasuta) eDNA survey results from the St. Francis River, Missouri 2018
Data describe a designed environmental DNA (eDNA) survey for the detection of longnose darter (Percina nasuta) in field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater fish species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to...
Metabarcoding data from freshwater mussel eDNA collected in Clinch River, Virginia 2017 Metabarcoding data from freshwater mussel eDNA collected in Clinch River, Virginia 2017
Data from metabarcoding assays to detect a suite of mussel species using mitochondrial DNA regions of the cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit (ND1) genes sequences.
Mucket eDNA detection in Wallen's Bend, Clinch river, Tennessee, September 2019 Mucket eDNA detection in Wallen's Bend, Clinch river, Tennessee, September 2019
The data describe the technical performance of a designed qPCR assay for the detection of mucket (Actinonaias ligamentina) eDNA from field collected water samples. Parameters described include the sequences of the primers and probes used; the limit of detection and limit of quantification for the assay; a list of freshwater mussels species that were used to test specificity of the assay...
Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17a-ethinylestradiol) Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17a-ethinylestradiol)
Largemouth base histological development and transcriptomic changes in gonad tissue after early life stage exposure to Atrazine (1-Chloro-3-ethylamino-5-isopropylamino-2,4,6-triazine) or the model estrogen 17a-ethinylestradiol.
Asian carp eDNA and egg morphology data collected from Truman Reservoir tributaries, Missouri, USA, 2014 Asian carp eDNA and egg morphology data collected from Truman Reservoir tributaries, Missouri, USA, 2014
Ichthyoplankton tows samples collected from Truman reservoir tributaries evaluated for eDNA. Data also includes hydrologic and water quality parameters and morphology of grass carp eggs.
Filter Total Items: 49
Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio
Thiamine (vitamin B1) metabolism is an important driver of human and animal health and ecological functioning. Some organisms, including species of ferns, mollusks, and fish, contain thiamine-degrading enzymes known as thiaminases, and consumption of these organisms can lead to thiamine deficiency in the consumer. Consumption of fish containing thiaminase has led to elevated mortality...
Authors
Cathy Richter, Allison Evans, Scott A. Heppell, James L. Zajicek, Donald Tillitt
Grass carp reproduction in small tributaries of Truman Reservoir, Missouri: Implications for establishment in novel habitats Grass carp reproduction in small tributaries of Truman Reservoir, Missouri: Implications for establishment in novel habitats
Substantial work has been conducted to estimate the river length required for recruitment of invasive Grass Carp Ctenopharyngodon idella and bigheaded carps (Bighead Carp Hypophthalmichthys nobilis and Silver Carp H. molitrix); however, the distance upstream and size of stream required for spawning remain unclear. Adult Grass Carp are regularly captured in Harry S. Truman Reservoir...
Authors
Cari-Ann Hayer, Michael Bayless, Cathy Richter, Amy George, Duane Chapman
Declines in reproductive condition of male largemouth bass (Micropterus salmoides) following seasonal exposure to estrogenic endocrine-disrupting compounds Declines in reproductive condition of male largemouth bass (Micropterus salmoides) following seasonal exposure to estrogenic endocrine-disrupting compounds
Reproductive abnormalities, that could lead to possible effects at the population level, have been observed in wild fish throughout the United States, with high prevalence in largemouth bass (LMB; Micropterus salmoides) and smallmouth bass (Micropterus dolomieu). Estrone (E1) and atrazine (ATR) are common environmental contaminants often associated with agricultural land use. 17alpha...
Authors
Jessica Leet, Cathy Richter, Robert Gale, Donald Tillitt, Jill Jenkins
Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection
Disease outbreaks, skin lesions, mortality events, and reproductive abnormalities have been observed in wild populations of centrarchids. The presence of estrogenic endocrine disrupting compounds (EEDCs) has been implicated as a potential causal factor for these effects. The effects of prior EEDC exposure on immune response were examined in juvenile largemouth bass (Micropterus salmoides...
Authors
Jessica Leet, Justin Greer, Cathy Richter, Luke R. Iwanowicz, Edward Spinard, Jacquelyn McDonald, Carla Conway, Robert Gale, Donald Tillitt, John Hansen
Using structured decision making to evaluate potential management responses to detection of dreissenid mussel (Dreissena spp.) environmental DNA Using structured decision making to evaluate potential management responses to detection of dreissenid mussel (Dreissena spp.) environmental DNA
Environmental (e)DNA tools are sensitive and cost-effective for early detection of invasive species. However, the uncertainty associated with the interpretation of positive eDNA detections makes it challenging to determine appropriate natural resource management responses. Multiple sources of error can give rise to positive detections of eDNA in a sample when individuals of that species...
Authors
Adam Sepulveda, David Smith, Katherine M. O'Donnell, Nathan Owens, Brittany White, Cathy Richter, Christopher Merkes, Skylar Wolf, Mike Rau, Matthew Neilson, Wesley M. Daniel, Christine Dumoulin, Margaret Hunter
A comparison of eDNA and visual survey methods for detection of longnose darter Percina nasuta in Missouri A comparison of eDNA and visual survey methods for detection of longnose darter Percina nasuta in Missouri
The longnose darter Percina nasuta is a rare and cryptic fish that recently disappeared from much of its historic range. We developed and used an environmental DNA (eDNA) assay for longnose darter paired with visual surveys to better determine the species’ range and compare detection probability between sampling approaches in an occupancy modeling framework. We detected longnose darter...
Authors
Jacob Westhoff, Leah Berkman, Katy Klymus, Nathan Thompson, Cathy Richter
Reproduction Reproduction
Lake charr Salvelinus namaycush are typically fall spawners although one ecotype has populations that spawn during spring and fall (siscowets in Lake Superior). Lake charr are iteroparous (reproduce more than once in a lifetime) with group-synchronous ovarian development and typically spawn once per year. However, lake charr may not reproduce every year, a phenomenon known as skipped...
Authors
Frederick W. Goetz, J. Ellen Marsden, Catherine Richter, Donald Tillitt, Shawn Sitar, Stephen Riley, Charles C. Krueger
Metabarcoding assays for the detection of freshwater mussels (Unionida) with environmental DNA Metabarcoding assays for the detection of freshwater mussels (Unionida) with environmental DNA
Freshwater mussels of the order Unionida are a widely distributed taxon that are important in maintaining freshwater ecosystems and are also highly imperiled throughout the world. Monitoring of mussel populations with environmental DNA (eDNA) is an attractive alternative to traditional methods because it is noninvasive and requires less labor and taxonomic knowledge from field personnel...
Authors
Katy Klymus, Catherine Richter, Nathan Thompson, Jo Hinck, Jess Jones
Development and testing of species-specific quantitative PCR assays for environmental DNA applications Development and testing of species-specific quantitative PCR assays for environmental DNA applications
New, non-invasive methods for detecting and monitoring species presence are being developed to aid in fisheries and wildlife conservation management. The use of environmental DNA (eDNA) samples for detecting macrobiota is one such group of methods that is rapidly becoming popular and being implemented in national management programs. Here we focus on the development of species-specific...
Authors
Katy Klymus, Dannise Ruiz-Ramos, Nathan Thompson, Catherine Richter
Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17α-ethinylestradiol) Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17α-ethinylestradiol)
Endocrine disrupting contaminants are of continuing concern for potentially contributing to reproductive dysfunction in largemouth and smallmouth bass in the Chesapeake Bay watershed (CBW) and elsewhere. Exposures to atrazine (ATR) have been hypothesized to have estrogenic effects on vertebrate endocrine systems. The incidence of intersex in male smallmouth bass from some regions of CBW...
Authors
Jessica Leet, Catherine Richter, Robert Cornman, Jason P. Berninger, Ramji K. Bhandari, Diane K. Nicks, James L. Zajicek, Vicki S. Blazer, Donald Tillitt
Use of environmental DNA to detect grass carp spawning events Use of environmental DNA to detect grass carp spawning events
The timing and location of spawning events are important data for managers seeking to control invasive grass carp populations. Ichthyoplankton tows for grass carp eggs and larvae can be used to detect spawning events; however, these samples can be highly debris-laden, and are expensive and laborious to process. An alternative method, environmental DNA (eDNA) technology, has proven...
Authors
Cari-Ann Hayer, Michael Bayless, Amy George, Nathan Thompson, Catherine Richter, Duane Chapman
Reporting the limits of detection and quantification for environmental DNA assays Reporting the limits of detection and quantification for environmental DNA assays
BackgroundEnvironmental DNA (eDNA) analysis is increasingly being used to detect the presence and relative abundance of rare species, especially invasive or imperiled aquatic species. The rapid progress in the eDNA field has resulted in numerous studies impacting conservation and management actions. However, standardization of eDNA methods and reporting across the field is yet to be...
Authors
Katy Klymus, Christopher Merkes, Michael Allison, Caren Goldberg, Caren Helbing, Margaret Hunter, Craig Jackson, Richard Lance, Anna M. Mangan, Emy Monroe, Antoinette Piaggio, Joel Stokdyk, Chris Wilson, Catherine Richter
Non-USGS Publications**
Taylor, J.A., Richter, C.A., Suzuki, A., Watanabe, H., Iguchi, T., Coser, K.R., Shioda, T., and vom Saal, F.S., 2012, Dose-related estrogen effects on gene expression in fetal mouse prostate mesenchymal cells: PLOS ONE, v. 7, no. 10, p. e48311
Taylor, J.A., Richter, C.A., Ruhlen, R.L., and vom Saal, F.S., 2011, Estrogenic environmental chemicals and drugs: Mechanisms for effects on the developing male urogenital system: The Journal of Steroid Biochemistry and Molecular Biology, v. 127, no. 1, p. 83-95
Richter, C.A., Taylor, J.A., Ruhlen, R.L., Welshons, W.V., and vom Saal, F.S., 2007, Estradiol and Bisphenol A stimulate androgen receptor and estrogen receptor gene expression in fetal mouse prostate mesenchyme cells: Environmental Health Perspectives, v. 115, no. 6, p. 902-908
vom Saal, F.S., Richter, C.A., Mao, J., and Welshons, W.V., 2005, Commercial animal feed: Variability in estrogenic activity and effects on body weight in mice: Birth Defects Research Part A: Clinical and Molecular Teratology, v. 73, no. 7, p. 474-475
Timms, B.G., Howdeshell, K.L., Barton, L., Bradley, S., Richter, C.A., and vom Saal, F.S., 2005, Estrogenic chemicals in plastic and oral contraceptives disrupt development of the fetal mouse prostate and urethra: Proceedings of the National Academy of Sciences of the United States of America, v. 102, no. 19, p. 7014-7019
Richter, C., Timms, B., and vom Saal, F., 2005, Prostate development: mechanisms for opposite effects of low and high doses of estrogenic chemicals, in Naz, R., ed., Endocrine Disruptors: Effects on Male and Female Reproductive Systems: New York, CRC Press, p. 379-410.
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