Donald Tillitt, PhD (Former Employee)
Science and Products
Filter Total Items: 215
Ethoxyresorufin-O-deethylase (EROD) activity in fish as a biomarker of chemical exposure Ethoxyresorufin-O-deethylase (EROD) activity in fish as a biomarker of chemical exposure
This review compiles and evaluates existing scientific information on the use, limitations, and procedural considerations for EROD activity (a catalytic measurement of cytochrome P4501A induction) as a biomarker in fish. A multitude of chemicals induce EROD activity in a variety of fish species, the most potent inducers being structural analogs of 2,3,7,8-tetracholordibenzo-p-dioxin...
Authors
J.J. Whyte, R.E. Jung, C. J. Schmitt, D. Tillitt
PCBs, liver lesions, and biomarker responses in adult walleye (Stizostedium vitreum vitreum) collected from Green Bay, Wisconsin PCBs, liver lesions, and biomarker responses in adult walleye (Stizostedium vitreum vitreum) collected from Green Bay, Wisconsin
Adult walleye were collected from several locations in the Lower Fox River and Green Bay, Wisconsin (the assessment area) and two relatively uncontaminated reference locations (Lake Winnebago and Patten Lake, Wisconsin) between July and October in 1996 and 1997. Whole body and liver samples collected in 1996 were analyzed for total PCBs, PCB congeners, and liver histological lesions...
Authors
Mace Barron, Michael Anderson, Dave Cacela, Joshua Lipton, Swee Teh, David Hinton, Judith Zelikoff, Audrey Dikkeboom, Donald Tillitt, Mark Holey, Nancy Denslow
Biomonitoring of Environmental Status and Trends (BEST) Program: Selected methods for monitoring chemical contaminants and their effects in aquatic ecosystems Biomonitoring of Environmental Status and Trends (BEST) Program: Selected methods for monitoring chemical contaminants and their effects in aquatic ecosystems
This document describes the suite of biological methods of the U.S. Geological Survey- Biomonitoring of Environmental Status and Trends program for monitoring chemical contaminants and their effects on fish. The methods, which were selected by panels of experts, are being field-tested in rivers of the Mississippi River, Columbia River, and Rio Grande basins. General health biomarkers...
Authors
Vicki S. Blazer, Nancy D. Denslow, Gail Dethloff, Timothy Gross, Kelly McDonald, Christopher Schmitt, Donald Tillitt, Jeffrey Whyte
Comparison of an enzyme-linked immunosorbent assay (ELISA) to gas chromatography (GC) - measurement of polychlorinated biphenyls (PCBs) in selected US fish extracts Comparison of an enzyme-linked immunosorbent assay (ELISA) to gas chromatography (GC) - measurement of polychlorinated biphenyls (PCBs) in selected US fish extracts
The analysis of PCBs in fish tissues by immunoassay methods was evaluated using fish collected from a US monitoring program, the National Contaminant Biomonitoring Program of the US Department of Interior, Fish and Wildlife Service. Selected composite whole fish samples, which represented widely varying concentrations and sources of PCBs, were extracted and subjected to congener PCB...
Authors
J.L. Zajicek, D. Tillitt, T.R. Schwartz, C. J. Schmitt, R.O. Harrison
Toxic responses of medaka, D-rR strain, to polychlorinatednaphthalene mixtures after embryonic exposure by in ovo nanoinjection: A partial life-cycle assessment Toxic responses of medaka, D-rR strain, to polychlorinatednaphthalene mixtures after embryonic exposure by in ovo nanoinjection: A partial life-cycle assessment
Polychlorinated naphthalenes (PCNs) are organic compounds with some chemical properties and uses similar to polychlorinated biphenyls. Polychlorinated naphthalenes have been detected in biota from certain aquatic environments. The toxicities of several PCN technical mixtures (Halowax) to medaka (Oryzias latipes) were determined by use of an embryo nanoinjection method. Medaka eggs (early...
Authors
Sergio Villalobos, Diana Papoulias, John Meadows, Alan Blankenship, Stephanie Pastva, Kurunthachalam Kannan, D.E. Hinton, Donald Tillitt, John Giesy
An in vivo model fish system to test chemical effects on sexual differentiation and development: exposure to ethinyl estradiol An in vivo model fish system to test chemical effects on sexual differentiation and development: exposure to ethinyl estradiol
A model system was characterized which may be used as an in vivo screen for effects of chemicals or environmental mixtures on sexual differentiation and development of reproductive organs and gametes. We evaluated the effects of a model environmental estrogen, ethinyl estradiol (EE2), on the d-rR strain of medaka, Oryzias latipes, using a nano-injection exposure. Gonad histopathology...
Authors
Diana Papoulias, Douglas Noltie, Donald Tillitt
Effects of methyl testosterone exposure on sexual differentiation in medaka, Oryzias latipes Effects of methyl testosterone exposure on sexual differentiation in medaka, Oryzias latipes
Studies were conducted to characterize effects of a known androgen on sexual differentiation and development of medaka, Oryzias latipes (d-rR strain), at two life stages. Embryos were injected with graded doses of methyl testosterone (MT) prior to epiboly. The occurrence of sex-reversal, and the gonadosomatic index (GSI) were evaluated in adults. Primary germ cells were counted and gonad...
Authors
D. M. Papoulias, Douglas Noltie, D. Tillitt
Evaluation of planar halogenated and polycyclic aromatic hydrocarbons in estuarine sediments using ethoxyresorufin-O-deethylase induction of H4IIE cells Evaluation of planar halogenated and polycyclic aromatic hydrocarbons in estuarine sediments using ethoxyresorufin-O-deethylase induction of H4IIE cells
Polycyclic aromatic hydrocarbons (PAHs) and planar halogenated hydrocarbons (PHHs), including polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), and biphenyls (PCBs) were determined in fractionated sediment extracts from the Hudson-Raritan estuary and Newark Bay, New Jersey, USA, as part of a comprehensive risk assessment. Contributions of PCDDs/PCDFs, PCBs, and PAHs to...
Authors
R.W. Gale, E.R. Long, T.R. Schwartz, D. Tillitt
Comparing polychlorinated biphenyl concentrations and patterns in the Saginaw River using sediment, caged fish, and semipermeable membrane devices Comparing polychlorinated biphenyl concentrations and patterns in the Saginaw River using sediment, caged fish, and semipermeable membrane devices
Three techniques of assessing bioavailable polychlorinated biphenyls (PCBs) in the Saginaw River, MI, were compared: sediments, caged fish, and semipermeable membrane devices (SPMDs). SPMDs and caged fish were placed in the river for 28 days at five sites where sediments were also sampled. The samples were analyzed for PCB congeners to determine concentrations and patterns. Total PCB...
Authors
K. Echols, R.W. Gale, T.R. Schwartz, J.N. Huckins, L.L. Williams, J.C. Meadows, D. Morse, J. Petty, C.E. Orazio, D. Tillitt
Progress and over-view of current GLFT EMS project: Report on Early Mortality Syndrome Workshop Progress and over-view of current GLFT EMS project: Report on Early Mortality Syndrome Workshop
No abstract available at this time
Authors
J. Fitzsimons, S.B. Brown, D. C. Honeyfield, D. Tillitt
Observed changes in early mortality syndrome in Lake Michigan coho and chinook salmon from 1994-1999 Observed changes in early mortality syndrome in Lake Michigan coho and chinook salmon from 1994-1999
No abstract available at this time
Authors
J. Hnath, M. Wolgamood, S.V. Marcquenski, S.B. Brown, J.D. Fitzsimons, D. Tillitt, D. C. Honeyfield
Dietary alewife and bloater chub effect on lake trout egg thiamine; Report on early mortality syndrome workshop Dietary alewife and bloater chub effect on lake trout egg thiamine; Report on early mortality syndrome workshop
No abstract available at this time
Authors
D. C. Honeyfield, J. Hinterkopf, J. Fitzsimons, S.B. Brown, D. Tillitt
Science and Products
Filter Total Items: 215
Ethoxyresorufin-O-deethylase (EROD) activity in fish as a biomarker of chemical exposure Ethoxyresorufin-O-deethylase (EROD) activity in fish as a biomarker of chemical exposure
This review compiles and evaluates existing scientific information on the use, limitations, and procedural considerations for EROD activity (a catalytic measurement of cytochrome P4501A induction) as a biomarker in fish. A multitude of chemicals induce EROD activity in a variety of fish species, the most potent inducers being structural analogs of 2,3,7,8-tetracholordibenzo-p-dioxin...
Authors
J.J. Whyte, R.E. Jung, C. J. Schmitt, D. Tillitt
PCBs, liver lesions, and biomarker responses in adult walleye (Stizostedium vitreum vitreum) collected from Green Bay, Wisconsin PCBs, liver lesions, and biomarker responses in adult walleye (Stizostedium vitreum vitreum) collected from Green Bay, Wisconsin
Adult walleye were collected from several locations in the Lower Fox River and Green Bay, Wisconsin (the assessment area) and two relatively uncontaminated reference locations (Lake Winnebago and Patten Lake, Wisconsin) between July and October in 1996 and 1997. Whole body and liver samples collected in 1996 were analyzed for total PCBs, PCB congeners, and liver histological lesions...
Authors
Mace Barron, Michael Anderson, Dave Cacela, Joshua Lipton, Swee Teh, David Hinton, Judith Zelikoff, Audrey Dikkeboom, Donald Tillitt, Mark Holey, Nancy Denslow
Biomonitoring of Environmental Status and Trends (BEST) Program: Selected methods for monitoring chemical contaminants and their effects in aquatic ecosystems Biomonitoring of Environmental Status and Trends (BEST) Program: Selected methods for monitoring chemical contaminants and their effects in aquatic ecosystems
This document describes the suite of biological methods of the U.S. Geological Survey- Biomonitoring of Environmental Status and Trends program for monitoring chemical contaminants and their effects on fish. The methods, which were selected by panels of experts, are being field-tested in rivers of the Mississippi River, Columbia River, and Rio Grande basins. General health biomarkers...
Authors
Vicki S. Blazer, Nancy D. Denslow, Gail Dethloff, Timothy Gross, Kelly McDonald, Christopher Schmitt, Donald Tillitt, Jeffrey Whyte
Comparison of an enzyme-linked immunosorbent assay (ELISA) to gas chromatography (GC) - measurement of polychlorinated biphenyls (PCBs) in selected US fish extracts Comparison of an enzyme-linked immunosorbent assay (ELISA) to gas chromatography (GC) - measurement of polychlorinated biphenyls (PCBs) in selected US fish extracts
The analysis of PCBs in fish tissues by immunoassay methods was evaluated using fish collected from a US monitoring program, the National Contaminant Biomonitoring Program of the US Department of Interior, Fish and Wildlife Service. Selected composite whole fish samples, which represented widely varying concentrations and sources of PCBs, were extracted and subjected to congener PCB...
Authors
J.L. Zajicek, D. Tillitt, T.R. Schwartz, C. J. Schmitt, R.O. Harrison
Toxic responses of medaka, D-rR strain, to polychlorinatednaphthalene mixtures after embryonic exposure by in ovo nanoinjection: A partial life-cycle assessment Toxic responses of medaka, D-rR strain, to polychlorinatednaphthalene mixtures after embryonic exposure by in ovo nanoinjection: A partial life-cycle assessment
Polychlorinated naphthalenes (PCNs) are organic compounds with some chemical properties and uses similar to polychlorinated biphenyls. Polychlorinated naphthalenes have been detected in biota from certain aquatic environments. The toxicities of several PCN technical mixtures (Halowax) to medaka (Oryzias latipes) were determined by use of an embryo nanoinjection method. Medaka eggs (early...
Authors
Sergio Villalobos, Diana Papoulias, John Meadows, Alan Blankenship, Stephanie Pastva, Kurunthachalam Kannan, D.E. Hinton, Donald Tillitt, John Giesy
An in vivo model fish system to test chemical effects on sexual differentiation and development: exposure to ethinyl estradiol An in vivo model fish system to test chemical effects on sexual differentiation and development: exposure to ethinyl estradiol
A model system was characterized which may be used as an in vivo screen for effects of chemicals or environmental mixtures on sexual differentiation and development of reproductive organs and gametes. We evaluated the effects of a model environmental estrogen, ethinyl estradiol (EE2), on the d-rR strain of medaka, Oryzias latipes, using a nano-injection exposure. Gonad histopathology...
Authors
Diana Papoulias, Douglas Noltie, Donald Tillitt
Effects of methyl testosterone exposure on sexual differentiation in medaka, Oryzias latipes Effects of methyl testosterone exposure on sexual differentiation in medaka, Oryzias latipes
Studies were conducted to characterize effects of a known androgen on sexual differentiation and development of medaka, Oryzias latipes (d-rR strain), at two life stages. Embryos were injected with graded doses of methyl testosterone (MT) prior to epiboly. The occurrence of sex-reversal, and the gonadosomatic index (GSI) were evaluated in adults. Primary germ cells were counted and gonad...
Authors
D. M. Papoulias, Douglas Noltie, D. Tillitt
Evaluation of planar halogenated and polycyclic aromatic hydrocarbons in estuarine sediments using ethoxyresorufin-O-deethylase induction of H4IIE cells Evaluation of planar halogenated and polycyclic aromatic hydrocarbons in estuarine sediments using ethoxyresorufin-O-deethylase induction of H4IIE cells
Polycyclic aromatic hydrocarbons (PAHs) and planar halogenated hydrocarbons (PHHs), including polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), and biphenyls (PCBs) were determined in fractionated sediment extracts from the Hudson-Raritan estuary and Newark Bay, New Jersey, USA, as part of a comprehensive risk assessment. Contributions of PCDDs/PCDFs, PCBs, and PAHs to...
Authors
R.W. Gale, E.R. Long, T.R. Schwartz, D. Tillitt
Comparing polychlorinated biphenyl concentrations and patterns in the Saginaw River using sediment, caged fish, and semipermeable membrane devices Comparing polychlorinated biphenyl concentrations and patterns in the Saginaw River using sediment, caged fish, and semipermeable membrane devices
Three techniques of assessing bioavailable polychlorinated biphenyls (PCBs) in the Saginaw River, MI, were compared: sediments, caged fish, and semipermeable membrane devices (SPMDs). SPMDs and caged fish were placed in the river for 28 days at five sites where sediments were also sampled. The samples were analyzed for PCB congeners to determine concentrations and patterns. Total PCB...
Authors
K. Echols, R.W. Gale, T.R. Schwartz, J.N. Huckins, L.L. Williams, J.C. Meadows, D. Morse, J. Petty, C.E. Orazio, D. Tillitt
Progress and over-view of current GLFT EMS project: Report on Early Mortality Syndrome Workshop Progress and over-view of current GLFT EMS project: Report on Early Mortality Syndrome Workshop
No abstract available at this time
Authors
J. Fitzsimons, S.B. Brown, D. C. Honeyfield, D. Tillitt
Observed changes in early mortality syndrome in Lake Michigan coho and chinook salmon from 1994-1999 Observed changes in early mortality syndrome in Lake Michigan coho and chinook salmon from 1994-1999
No abstract available at this time
Authors
J. Hnath, M. Wolgamood, S.V. Marcquenski, S.B. Brown, J.D. Fitzsimons, D. Tillitt, D. C. Honeyfield
Dietary alewife and bloater chub effect on lake trout egg thiamine; Report on early mortality syndrome workshop Dietary alewife and bloater chub effect on lake trout egg thiamine; Report on early mortality syndrome workshop
No abstract available at this time
Authors
D. C. Honeyfield, J. Hinterkopf, J. Fitzsimons, S.B. Brown, D. Tillitt
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government