Katy Klymus, PhD
Dr. Katy Klymus is a Research Biologist at the Columbia Environmental Research Center.
Since joining the USGS in 2016, Katy’s research focus has been on using genetic and genomic methods to inform wildlife species management. Currently she is studying environmental DNA methods, how they can be used to assess current levels of and changes in biodiversity, and development of standards for eDNA analyses. Additionally, she has a background in ecology, animal behavior and phylogenetics/ systematics.
Professional Experience
2016 - Present: Research Biologist, U.S. Geological Survey, Columbia Environmental Research Center
2014 - 2016: Post-Doctoral Fellow, Lake Erie Center, University of Toledo
2012 - 2014: Post-Doctoral Researcher, Missouri Cooperative Fish and Wildlife Research Unit, University of Missouri
Education and Certifications
Ph.D. Evolutionary Biology and Ecology, University of Missouri, 2011
B.S. Zoology, University of Texas, 2000
Science and Products
Filter Total Items: 22
Environmental DNA shedding rates in laboratory conditions for Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea. Environmental DNA shedding rates in laboratory conditions for Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea.
Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for the spectaclecase (Cumberlandia monodonta), mucket (Actinonaias ligamentina), and fatmucket (Lampsilis siliquoidea) from water samples collected in the laboratory under differing laboratory conditions. Shedding rates were tested under variable mussel biomass, diet, and temperature...
Water chemistry and molecular eDNA data observed in experimental laboratory mesocosms exposed to different nitrogen amendments in the presence or absence of a nitrifier enriched microbial community Water chemistry and molecular eDNA data observed in experimental laboratory mesocosms exposed to different nitrogen amendments in the presence or absence of a nitrifier enriched microbial community
Data describe the results of a controlled laboratory mesocosm experiment evaluating the influence of a nitrifier enriched microbial community on silver carp (Hypophthalmichthys molitrix) milt eDNA degradation. Parameters described include the concentration results, limit of detection, and limit of quantification of two silver carp specific quantitative PCR assays and water chemistry...
Data on the detection of Epioblasma capsaeformis using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Clinch River, Tennessee (2019 to 2021) Data on the detection of Epioblasma capsaeformis using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Clinch River, Tennessee (2019 to 2021)
Data describe a designed environmental DNA (eDNA) survey for the detection of oyster mussel (Epioblasma capsaeformis) from field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater mussel species tissue samples that were used to test specificity of the assay; and field collected water samples that...
Data on the detection of Cumberlandia monodonta using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Big Piney River, Missouri 2020 – 2022 (ver. 2.0, August 2024) Data on the detection of Cumberlandia monodonta using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Big Piney River, Missouri 2020 – 2022 (ver. 2.0, August 2024)
Data describe a designed environmental DNA (eDNA) survey for the detection of Spectaclecase (Cumberlandia monodonta, also referred to as Margaritifera monodonta) from field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater mussel species tissue samples that were used to test specificity of the assay...
Degradation of Cumberlandia monodonta environmental DNA over time in laboratory conditions using the C.monCOI assay Degradation of Cumberlandia monodonta environmental DNA over time in laboratory conditions using the C.monCOI assay
Data describe a designed environmental DNA (eDNA) experiment for the estimation of eDNA degradation rates for the spectaclecase (Cumberlandia monodonta) from water samples collected in the laboratory. Parameters described include laboratory collected water samples that were tested to estimate concentration of spectaclecase DNA and associated quality assurance data. Samples were collected...
Genomic variation in the genus Pimephales: raw sequence data and single-nucleotide polymorphisms Genomic variation in the genus Pimephales: raw sequence data and single-nucleotide polymorphisms
The fathead minnow (Pimephales promelas) is a key model of vertebrate toxicity. Standardized tests of toxicity in fathead minnow have been developed to support regulatory science, and much is known about the response of the species to various environmental pollutants. However, there is little data on genetic variation within the species, despite the potential influence of genetic...
Longnose darter (Percina nasuta) eDNA survey results from the St. Francis River, Missouri 2018 Longnose darter (Percina nasuta) eDNA survey results from the St. Francis River, Missouri 2018
Data describe a designed environmental DNA (eDNA) survey for the detection of longnose darter (Percina nasuta) in field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater fish species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to...
Metabarcoding data from freshwater mussel eDNA collected in Clinch River, Virginia 2017 Metabarcoding data from freshwater mussel eDNA collected in Clinch River, Virginia 2017
Data from metabarcoding assays to detect a suite of mussel species using mitochondrial DNA regions of the cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit (ND1) genes sequences.
Mucket eDNA detection in Wallen's Bend, Clinch river, Tennessee, September 2019 Mucket eDNA detection in Wallen's Bend, Clinch river, Tennessee, September 2019
The data describe the technical performance of a designed qPCR assay for the detection of mucket (Actinonaias ligamentina) eDNA from field collected water samples. Parameters described include the sequences of the primers and probes used; the limit of detection and limit of quantification for the assay; a list of freshwater mussels species that were used to test specificity of the assay...
eDNA sampling sites in the Grand Canyon region near breccia pipe uranium mines_2015_2016 eDNA sampling sites in the Grand Canyon region near breccia pipe uranium mines_2015_2016
Geospatial locations of eDNA sampling sites in the Grand Canyon region near breccia pipe uranium mines
Filter Total Items: 29
The Government eDNA Working Group 6th Annual eDNA Technical Exchange Workshop The Government eDNA Working Group 6th Annual eDNA Technical Exchange Workshop
The 6th annual Environmental DNA (eDNA) Technical Exchange Workshop was a virtual workshop hosted and coordinated by the Government eDNA Working Group (GEDWG) on January 24–26, 2023. GEDWG is a no-cost consortium that focuses on bringing together stakeholders associated with federal, state, provincial, municipal, and other government and non-government agencies interested in eDNA and...
Authors
Carol Stepien, Richard Lance, Katy Klymus, Margaret Hunter
Gaining decision-maker confidence through community consensus: Developing environmental DNA standards for data display on the USGS Nonindigenous Aquatic Species database Gaining decision-maker confidence through community consensus: Developing environmental DNA standards for data display on the USGS Nonindigenous Aquatic Species database
To advance national efforts for the detection and biosurveillance of aquatic invasive species (AIS), we employed a community consensus process to enable the incorporation of environmental DNA (eDNA) detection data into the U.S. Geological Survey’s (USGS) Nonindigenous Aquatic Species (NAS) database (https://nas.er.usgs.gov/eDNA/). Our goal was to identify minimum standards and best...
Authors
Jason Ferrante, Wesley M. Daniel, Jonathan Freedman, Katy Klymus, Matthew Neilson, Yale Passamaneck, Christopher B. Rees, Adam Sepulveda, Margaret Hunter
Genome resequencing clarifies phylogeny and reveals patterns of selection in the toxicogenomics model Pimephales promelas Genome resequencing clarifies phylogeny and reveals patterns of selection in the toxicogenomics model Pimephales promelas
Background The fathead minnow (Pimephales promelas) is a model species for toxicological research. A high-quality genome reference sequence is available, and genomic methods are increasingly used in toxicological studies of the species. However, phylogenetic relationships within the genus remain incompletely known and little population-genomic data are available for fathead minnow...
Authors
Katy Klymus, Robert Hrabik, Nathan Thompson, Robert Cornman
A comparison of eDNA and visual survey methods for detection of longnose darter Percina nasuta in Missouri A comparison of eDNA and visual survey methods for detection of longnose darter Percina nasuta in Missouri
The longnose darter Percina nasuta is a rare and cryptic fish that recently disappeared from much of its historic range. We developed and used an environmental DNA (eDNA) assay for longnose darter paired with visual surveys to better determine the species’ range and compare detection probability between sampling approaches in an occupancy modeling framework. We detected longnose darter...
Authors
Jacob Westhoff, Leah Berkman, Katy Klymus, Nathan Thompson, Cathy Richter
Editorial: Environmental DNA innovations for conservation Editorial: Environmental DNA innovations for conservation
Environmental DNA (eDNA) analysis refers to the collection of bulk environmental samples such as water, sediment, or air, and studying the genetic remnants that organisms have shed into their environment to gain information about species presence.
Authors
Matthew A Barnes, Katy Klymus, Hiroki Yamanaka
Metabarcoding assays for the detection of freshwater mussels (Unionida) with environmental DNA Metabarcoding assays for the detection of freshwater mussels (Unionida) with environmental DNA
Freshwater mussels of the order Unionida are a widely distributed taxon that are important in maintaining freshwater ecosystems and are also highly imperiled throughout the world. Monitoring of mussel populations with environmental DNA (eDNA) is an attractive alternative to traditional methods because it is noninvasive and requires less labor and taxonomic knowledge from field personnel...
Authors
Katy Klymus, Catherine Richter, Nathan Thompson, Jo Hinck, Jess Jones
Development and testing of species-specific quantitative PCR assays for environmental DNA applications Development and testing of species-specific quantitative PCR assays for environmental DNA applications
New, non-invasive methods for detecting and monitoring species presence are being developed to aid in fisheries and wildlife conservation management. The use of environmental DNA (eDNA) samples for detecting macrobiota is one such group of methods that is rapidly becoming popular and being implemented in national management programs. Here we focus on the development of species-specific...
Authors
Katy Klymus, Dannise Ruiz-Ramos, Nathan Thompson, Catherine Richter
Reporting the limits of detection and quantification for environmental DNA assays Reporting the limits of detection and quantification for environmental DNA assays
Background Environmental DNA (eDNA) analysis is increasingly being used to detect the presence and relative abundance of rare species, especially invasive or imperiled aquatic species. The rapid progress in the eDNA field has resulted in numerous studies impacting conservation and management actions. However, standardization of eDNA methods and reporting across the field is yet to be...
Authors
Katy Klymus, Christopher Merkes, Michael Allison, Caren Goldberg, Caren Helbing, Margaret Hunter, Craig Jackson, Richard Lance, Anna M. Mangan, Emy Monroe, Antoinette Piaggio, Joel Stokdyk, Chris Wilson, Catherine Richter
Metabarcoding of environmental DNA samples to explore the use of uranium mine containment ponds as a water source for wildlife Metabarcoding of environmental DNA samples to explore the use of uranium mine containment ponds as a water source for wildlife
Understanding how anthropogenic impacts on the landscape affect wildlife requires a knowledge of community assemblages. Species surveys are the first step in assessing community structure, and recent molecular applications such as metabarcoding and environmental DNA analyses have been proposed as an additional and complementary wildlife survey method. Here, we test eDNA metabarcoding as...
Authors
Katy Klymus, Catherine Richter, Nathan Thompson, Jo Hinck
Experimental observations on the decay of environmental DNA from bighead and silver carps Experimental observations on the decay of environmental DNA from bighead and silver carps
Interest in the field of environmental DNA (eDNA) is growing rapidly and eDNA surveys are becoming an important consideration for aquatic resource managers dealing with invasive species. However, in order for eDNA monitoring to mature as a research and management tool, there are several critical knowledge gaps that must be filled. One such gap is the fate of eDNA materials in the aquatic
Authors
Richard Lance, Katy Klymus, Catherine Richter, Xin Guan, Heather Farrington, Matthew Carr, Nathan Thompson, Duane Chapman, Kelly Baerwaldt
Critical considerations for the application of environmental DNA methods to detect aquatic species Critical considerations for the application of environmental DNA methods to detect aquatic species
Species detection using environmental DNA (eDNA) has tremendous potential for contributing to the understanding of the ecology and conservation of aquatic species. Detecting species using eDNA methods, rather than directly sampling the organisms, can reduce impacts on sensitive species and increase the power of field surveys for rare and elusive species. The sensitivity of eDNA methods...
Authors
Caren Goldberg, Cameron Turner, Kristy Deiner, Katy Klymus, Philip Thomsen, Melanie Murphy, Stephen Spear, Anna McKee, Sara Oyler-McCance, Robert Cornman, Matthew Laramie, Andrew R. Mahon, Richard Lance, David S. Pilliod, Katherine Strickler, Lisette Waits, Alexander K. Fremier, Teruhiko Takahara, Jelger Herder, Pierre Taberlet
Environmental DNA calibration study interim technical review report December 2014 Environmental DNA calibration study interim technical review report December 2014
The Environmental DNA Calibration Study (ECALS) is a multi-year study to improve the understanding and interpretation of the detection of Asian carp DNA in environmental samples (eDNA) used in early detection monitoring. eDNA surveillance programs seek to detect the presence of genetic material (DNA in cells sloughed off in slime, feces, urine, etc.) extracted from water samples; the...
Authors
Kelly Baerwaldt, Meredith Bartron, Kurt Schilling, Debbie Lee, Edmond Russo, Trudy Estes, Richard Fischer, Beth Fleming, Michael Guilfoyle, Jack Killgore, Richard Lance, Edward Perkins, Martin Schultz, David Smith, Jon Amberg, Duane Chapman, Mark Gaikowski, Katy Klymus, Catherine Richter
Non-USGS Publications**
Soamiarimampionona, J., Sam, S.S., Dolch, R., Klymus, K., Rabemananjara, F., Robsomanitrandrasana, E., Rakotoarisoa, J.C., and Edmonds, D., 2015, Effects of three diets on development of Mantidactylus betsileanus larvae in captivity: Alytes, v. 32, p. 7-15
Klymus, K.E., and Carl Gerhardt, H., 2012, AFLP markers resolve intra-specific relationships and infer genetic structure among lineages of the canyon treefrog, Hyla arenicolor: Molecular Phylogenetics and Evolution, v. 65, no. 2, p. 654-667
Klymus, K.E., Humfeld, S.C., and Gerhardt, H.C., 2012, Geographical variation in male advertisement calls and female preference of the wide-ranging canyon treefrog, Hyla arenicolor: Biological Journal of the Linnean Society, v. 107, no. 1, p. 219-232
Klymus, K.E., Humfeld, S.C., Marshall, V.T., Cannatella, D., and Gerhardt, H.C., 2010, Molecular patterns of differentiation in canyon treefrogs (Hyla arenicolor): evidence for introgressive hybridization with the Arizona treefrog (H. wrightorum) and correlations with advertisement call differences: Journal of Evolutionary Biology, v. 23, no. 7, p. 1425-1435
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Science and Products
Filter Total Items: 22
Environmental DNA shedding rates in laboratory conditions for Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea. Environmental DNA shedding rates in laboratory conditions for Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea.
Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for the spectaclecase (Cumberlandia monodonta), mucket (Actinonaias ligamentina), and fatmucket (Lampsilis siliquoidea) from water samples collected in the laboratory under differing laboratory conditions. Shedding rates were tested under variable mussel biomass, diet, and temperature...
Water chemistry and molecular eDNA data observed in experimental laboratory mesocosms exposed to different nitrogen amendments in the presence or absence of a nitrifier enriched microbial community Water chemistry and molecular eDNA data observed in experimental laboratory mesocosms exposed to different nitrogen amendments in the presence or absence of a nitrifier enriched microbial community
Data describe the results of a controlled laboratory mesocosm experiment evaluating the influence of a nitrifier enriched microbial community on silver carp (Hypophthalmichthys molitrix) milt eDNA degradation. Parameters described include the concentration results, limit of detection, and limit of quantification of two silver carp specific quantitative PCR assays and water chemistry...
Data on the detection of Epioblasma capsaeformis using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Clinch River, Tennessee (2019 to 2021) Data on the detection of Epioblasma capsaeformis using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Clinch River, Tennessee (2019 to 2021)
Data describe a designed environmental DNA (eDNA) survey for the detection of oyster mussel (Epioblasma capsaeformis) from field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater mussel species tissue samples that were used to test specificity of the assay; and field collected water samples that...
Data on the detection of Cumberlandia monodonta using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Big Piney River, Missouri 2020 – 2022 (ver. 2.0, August 2024) Data on the detection of Cumberlandia monodonta using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Big Piney River, Missouri 2020 – 2022 (ver. 2.0, August 2024)
Data describe a designed environmental DNA (eDNA) survey for the detection of Spectaclecase (Cumberlandia monodonta, also referred to as Margaritifera monodonta) from field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater mussel species tissue samples that were used to test specificity of the assay...
Degradation of Cumberlandia monodonta environmental DNA over time in laboratory conditions using the C.monCOI assay Degradation of Cumberlandia monodonta environmental DNA over time in laboratory conditions using the C.monCOI assay
Data describe a designed environmental DNA (eDNA) experiment for the estimation of eDNA degradation rates for the spectaclecase (Cumberlandia monodonta) from water samples collected in the laboratory. Parameters described include laboratory collected water samples that were tested to estimate concentration of spectaclecase DNA and associated quality assurance data. Samples were collected...
Genomic variation in the genus Pimephales: raw sequence data and single-nucleotide polymorphisms Genomic variation in the genus Pimephales: raw sequence data and single-nucleotide polymorphisms
The fathead minnow (Pimephales promelas) is a key model of vertebrate toxicity. Standardized tests of toxicity in fathead minnow have been developed to support regulatory science, and much is known about the response of the species to various environmental pollutants. However, there is little data on genetic variation within the species, despite the potential influence of genetic...
Longnose darter (Percina nasuta) eDNA survey results from the St. Francis River, Missouri 2018 Longnose darter (Percina nasuta) eDNA survey results from the St. Francis River, Missouri 2018
Data describe a designed environmental DNA (eDNA) survey for the detection of longnose darter (Percina nasuta) in field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater fish species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to...
Metabarcoding data from freshwater mussel eDNA collected in Clinch River, Virginia 2017 Metabarcoding data from freshwater mussel eDNA collected in Clinch River, Virginia 2017
Data from metabarcoding assays to detect a suite of mussel species using mitochondrial DNA regions of the cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit (ND1) genes sequences.
Mucket eDNA detection in Wallen's Bend, Clinch river, Tennessee, September 2019 Mucket eDNA detection in Wallen's Bend, Clinch river, Tennessee, September 2019
The data describe the technical performance of a designed qPCR assay for the detection of mucket (Actinonaias ligamentina) eDNA from field collected water samples. Parameters described include the sequences of the primers and probes used; the limit of detection and limit of quantification for the assay; a list of freshwater mussels species that were used to test specificity of the assay...
eDNA sampling sites in the Grand Canyon region near breccia pipe uranium mines_2015_2016 eDNA sampling sites in the Grand Canyon region near breccia pipe uranium mines_2015_2016
Geospatial locations of eDNA sampling sites in the Grand Canyon region near breccia pipe uranium mines
Filter Total Items: 29
The Government eDNA Working Group 6th Annual eDNA Technical Exchange Workshop The Government eDNA Working Group 6th Annual eDNA Technical Exchange Workshop
The 6th annual Environmental DNA (eDNA) Technical Exchange Workshop was a virtual workshop hosted and coordinated by the Government eDNA Working Group (GEDWG) on January 24–26, 2023. GEDWG is a no-cost consortium that focuses on bringing together stakeholders associated with federal, state, provincial, municipal, and other government and non-government agencies interested in eDNA and...
Authors
Carol Stepien, Richard Lance, Katy Klymus, Margaret Hunter
Gaining decision-maker confidence through community consensus: Developing environmental DNA standards for data display on the USGS Nonindigenous Aquatic Species database Gaining decision-maker confidence through community consensus: Developing environmental DNA standards for data display on the USGS Nonindigenous Aquatic Species database
To advance national efforts for the detection and biosurveillance of aquatic invasive species (AIS), we employed a community consensus process to enable the incorporation of environmental DNA (eDNA) detection data into the U.S. Geological Survey’s (USGS) Nonindigenous Aquatic Species (NAS) database (https://nas.er.usgs.gov/eDNA/). Our goal was to identify minimum standards and best...
Authors
Jason Ferrante, Wesley M. Daniel, Jonathan Freedman, Katy Klymus, Matthew Neilson, Yale Passamaneck, Christopher B. Rees, Adam Sepulveda, Margaret Hunter
Genome resequencing clarifies phylogeny and reveals patterns of selection in the toxicogenomics model Pimephales promelas Genome resequencing clarifies phylogeny and reveals patterns of selection in the toxicogenomics model Pimephales promelas
Background The fathead minnow (Pimephales promelas) is a model species for toxicological research. A high-quality genome reference sequence is available, and genomic methods are increasingly used in toxicological studies of the species. However, phylogenetic relationships within the genus remain incompletely known and little population-genomic data are available for fathead minnow...
Authors
Katy Klymus, Robert Hrabik, Nathan Thompson, Robert Cornman
A comparison of eDNA and visual survey methods for detection of longnose darter Percina nasuta in Missouri A comparison of eDNA and visual survey methods for detection of longnose darter Percina nasuta in Missouri
The longnose darter Percina nasuta is a rare and cryptic fish that recently disappeared from much of its historic range. We developed and used an environmental DNA (eDNA) assay for longnose darter paired with visual surveys to better determine the species’ range and compare detection probability between sampling approaches in an occupancy modeling framework. We detected longnose darter...
Authors
Jacob Westhoff, Leah Berkman, Katy Klymus, Nathan Thompson, Cathy Richter
Editorial: Environmental DNA innovations for conservation Editorial: Environmental DNA innovations for conservation
Environmental DNA (eDNA) analysis refers to the collection of bulk environmental samples such as water, sediment, or air, and studying the genetic remnants that organisms have shed into their environment to gain information about species presence.
Authors
Matthew A Barnes, Katy Klymus, Hiroki Yamanaka
Metabarcoding assays for the detection of freshwater mussels (Unionida) with environmental DNA Metabarcoding assays for the detection of freshwater mussels (Unionida) with environmental DNA
Freshwater mussels of the order Unionida are a widely distributed taxon that are important in maintaining freshwater ecosystems and are also highly imperiled throughout the world. Monitoring of mussel populations with environmental DNA (eDNA) is an attractive alternative to traditional methods because it is noninvasive and requires less labor and taxonomic knowledge from field personnel...
Authors
Katy Klymus, Catherine Richter, Nathan Thompson, Jo Hinck, Jess Jones
Development and testing of species-specific quantitative PCR assays for environmental DNA applications Development and testing of species-specific quantitative PCR assays for environmental DNA applications
New, non-invasive methods for detecting and monitoring species presence are being developed to aid in fisheries and wildlife conservation management. The use of environmental DNA (eDNA) samples for detecting macrobiota is one such group of methods that is rapidly becoming popular and being implemented in national management programs. Here we focus on the development of species-specific...
Authors
Katy Klymus, Dannise Ruiz-Ramos, Nathan Thompson, Catherine Richter
Reporting the limits of detection and quantification for environmental DNA assays Reporting the limits of detection and quantification for environmental DNA assays
Background Environmental DNA (eDNA) analysis is increasingly being used to detect the presence and relative abundance of rare species, especially invasive or imperiled aquatic species. The rapid progress in the eDNA field has resulted in numerous studies impacting conservation and management actions. However, standardization of eDNA methods and reporting across the field is yet to be...
Authors
Katy Klymus, Christopher Merkes, Michael Allison, Caren Goldberg, Caren Helbing, Margaret Hunter, Craig Jackson, Richard Lance, Anna M. Mangan, Emy Monroe, Antoinette Piaggio, Joel Stokdyk, Chris Wilson, Catherine Richter
Metabarcoding of environmental DNA samples to explore the use of uranium mine containment ponds as a water source for wildlife Metabarcoding of environmental DNA samples to explore the use of uranium mine containment ponds as a water source for wildlife
Understanding how anthropogenic impacts on the landscape affect wildlife requires a knowledge of community assemblages. Species surveys are the first step in assessing community structure, and recent molecular applications such as metabarcoding and environmental DNA analyses have been proposed as an additional and complementary wildlife survey method. Here, we test eDNA metabarcoding as...
Authors
Katy Klymus, Catherine Richter, Nathan Thompson, Jo Hinck
Experimental observations on the decay of environmental DNA from bighead and silver carps Experimental observations on the decay of environmental DNA from bighead and silver carps
Interest in the field of environmental DNA (eDNA) is growing rapidly and eDNA surveys are becoming an important consideration for aquatic resource managers dealing with invasive species. However, in order for eDNA monitoring to mature as a research and management tool, there are several critical knowledge gaps that must be filled. One such gap is the fate of eDNA materials in the aquatic
Authors
Richard Lance, Katy Klymus, Catherine Richter, Xin Guan, Heather Farrington, Matthew Carr, Nathan Thompson, Duane Chapman, Kelly Baerwaldt
Critical considerations for the application of environmental DNA methods to detect aquatic species Critical considerations for the application of environmental DNA methods to detect aquatic species
Species detection using environmental DNA (eDNA) has tremendous potential for contributing to the understanding of the ecology and conservation of aquatic species. Detecting species using eDNA methods, rather than directly sampling the organisms, can reduce impacts on sensitive species and increase the power of field surveys for rare and elusive species. The sensitivity of eDNA methods...
Authors
Caren Goldberg, Cameron Turner, Kristy Deiner, Katy Klymus, Philip Thomsen, Melanie Murphy, Stephen Spear, Anna McKee, Sara Oyler-McCance, Robert Cornman, Matthew Laramie, Andrew R. Mahon, Richard Lance, David S. Pilliod, Katherine Strickler, Lisette Waits, Alexander K. Fremier, Teruhiko Takahara, Jelger Herder, Pierre Taberlet
Environmental DNA calibration study interim technical review report December 2014 Environmental DNA calibration study interim technical review report December 2014
The Environmental DNA Calibration Study (ECALS) is a multi-year study to improve the understanding and interpretation of the detection of Asian carp DNA in environmental samples (eDNA) used in early detection monitoring. eDNA surveillance programs seek to detect the presence of genetic material (DNA in cells sloughed off in slime, feces, urine, etc.) extracted from water samples; the...
Authors
Kelly Baerwaldt, Meredith Bartron, Kurt Schilling, Debbie Lee, Edmond Russo, Trudy Estes, Richard Fischer, Beth Fleming, Michael Guilfoyle, Jack Killgore, Richard Lance, Edward Perkins, Martin Schultz, David Smith, Jon Amberg, Duane Chapman, Mark Gaikowski, Katy Klymus, Catherine Richter
Non-USGS Publications**
Soamiarimampionona, J., Sam, S.S., Dolch, R., Klymus, K., Rabemananjara, F., Robsomanitrandrasana, E., Rakotoarisoa, J.C., and Edmonds, D., 2015, Effects of three diets on development of Mantidactylus betsileanus larvae in captivity: Alytes, v. 32, p. 7-15
Klymus, K.E., and Carl Gerhardt, H., 2012, AFLP markers resolve intra-specific relationships and infer genetic structure among lineages of the canyon treefrog, Hyla arenicolor: Molecular Phylogenetics and Evolution, v. 65, no. 2, p. 654-667
Klymus, K.E., Humfeld, S.C., and Gerhardt, H.C., 2012, Geographical variation in male advertisement calls and female preference of the wide-ranging canyon treefrog, Hyla arenicolor: Biological Journal of the Linnean Society, v. 107, no. 1, p. 219-232
Klymus, K.E., Humfeld, S.C., Marshall, V.T., Cannatella, D., and Gerhardt, H.C., 2010, Molecular patterns of differentiation in canyon treefrogs (Hyla arenicolor): evidence for introgressive hybridization with the Arizona treefrog (H. wrightorum) and correlations with advertisement call differences: Journal of Evolutionary Biology, v. 23, no. 7, p. 1425-1435
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.