Images

Juga sp. snails, the molluscan intermediate host for Nanophyetus salmincola, populate the bottom of a stream bed in a south Puget Sound watershed.

Flushing stomach contents from a live resident Chinook salmon for an investigation of cannibalism and predation impacts.

Gills from Lost River suckers with a heavy infestation of Ichthyobodo sp. (arrows).  Slide is stained with hematoxylin and eosin.

Herring (top) and juvenile Chinook salmon (bottom) flushed from the stomach of a resident Chinook salmon in Puget Sound.

Nested polymerase chain reaction (nPCR) for the detection of Renibacterium salmoninarum. Agarose gel electrophoresis is used for size separation and visualization of amplified DNA sequences.

Lost River suckers congregating to spawn on Sucker Springs in Upper Klamath Lake, Oregon.

Nested polymerase chain reaction (nPCR) for the detection of Renibacterium salmoninarum. Agarose gel electrophoresis is used for size separation and visualization of amplified DNA sequences.

Nested polymerase chain reaction (nPCR) for the detection of Renibacterium salmoninarum. Following two rounds of PCR amplification, samples are pipetted into an agarose gel for electrophoresis.

Sampling Striped Bass for diet items in the San Francisco Estuary.

Spread plate procedure for quantitative culture Renibacterium salmoninarum from fish tissue or ovarian fluid.

Passive Integrated Transponder (PIT) tag detection station on Willow Creek, California. Systems like this one are used to detect movements of endangered Lost River and shortnose suckers in remote locations.

Tissue sections are mounted on glass slides, stained and examined with a microscope that magnifies cellular details up to 2,000 times with brightfield or fluorescence imaging. Microscopes are used in our research to understand the pathological changes caused by infectious agents such as bacteria, fungi, parasites and viruses.

Quantitative Polymerase Chain Reaction (qPCR) assay for the detection of Renibacterium salmoninarum DNA. A nucleic acid sequence detection system is shown.

Quantitative polymerase chain reaction (qPCR) assay for the detection of Renibacterium salmoninarum DNA. Observation of qPCR results on the screen of a sequence detection system.

Hells Canyon on the Snake River.

Direct fluorescent antibody test (DFAT) for the detection of Renibacterium salmoninarum in tissues. Fluorescing R. salmoninarum cells are visible on a slide.

The Cle Elum River, a tributary of the Yakima River in Washington state. Sockeye salmon reintroduction efforts were initiated in the Cle Elum River by the Yakama Nation and Washington Department of Fish and Wildlife in 2009.

Sockeye salmon preparing to spawn upstream of Cle Elum Dam, Washington.

As blooms of cyanobacteria die on the water surface of Upper Klamath Lake they turn blue-green.

USGS studies the behavior and health of juvenile endangered Lost River and shortnose suckers within in-lake mesocosms in Upper Klamath Lake, Oregon.

Shoreline traps in Lookout Point Reservoir, Oregon.