Western Fisheries Research Center

Title: Double polyclonal enzyme-linked immunosorbent assay (ELISA) for the detection of Renibacterium salmonarium

Number: BACT-2

Version: 02

Purpose: To detect Renibacterium salmoninarum soluble antigen in fish tissues or fluids

Title: Direct Fluorescent Antibody Test (DFAT) for the detection of Renibacterium salmoninarum in tissues

Number: BACT-3

Version: 02 Created December 14, 2011

Purpose: Detection of Renibacterium salmoninarum cells in fish tissues or culture

Title: Membrane-Filtration Fluorescent Antibody Text (MF-FAT) for the detection of Renibacterium salmoninarum

Number: BACT-4

Version: 02 Created January 10, 2012

Purpose: Detection of Renibacterium salmoninarum in fish fluids or broth culture

Title: DNA Extraction for PCR-based Detection of Renibacterium salmoninarum

Number: BACT-5

Version: 01 Created April 15, 2011

Purpose: Recommended DNA extraction procedure of fish tissues and ovarian fluids for PCR-based detection of Renibacterium salmoninarum

Title: Nested Polymerase Chain Reaction (nPCR) for the detection of Renibacterium salmoninarum

Number: BACT-6

Version: 01 Created April 15, 2011

Purpose: To detect nucleic acids from Renibacterium salmoninarum in DNA extracted from fish tissues or fluids using a nested PCR primer set

Title: Quantitative Polymerase Chain Reaction (qPCR) for the detection of Renibacterium salmoninarum

Number: BACT-7

Version: 02 Created December 14, 2011

Purpose: To detect nucleic acids from Renibacterium salmoninarum in DNA extracted from fish tissues or fluids using quantitative PCR

Western Fisheries Science News, February 2019 | Issue 7.2 (pdf)

Western Fisheries Science News, May 2019 | Issue 7.5

Title: Culture of Renibacterium salmoninarum from Tissues and Ovarian Fluid

Number: BACT-1

Version: 01 Created April 19, 2011

Purpose: Culture of Renibacterium salmoninarum in fish tissues or ovarian fluids