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Pacific Northwest Environmental DNA Laboratory

We are a premier environmental DNA (eDNA) laboratory supporting the analytical and technical eDNA needs of federal and non-federal natural resources management, conservation, and restoration entities in the western U.S. 



New Effort - Using Artificial Flowers to Survey for Pollinators at Solar Installations


Bipartisan Infrastructure Law investments combine science and technology to track biological threats in US waters


Detecting Invasive and Rare Species with the National Streamflow Network


It’s complicated…environmental DNA as a predictor of trout and char abundance in streams

The potential to provide inferences about fish abundance from environmental (e)DNA samples has generated great interest. However, the accuracy of these abundance estimates is often low and variable across species and space. A plausible refinement is the use of common aquatic habitat monitoring data to account for attributes that influence eDNA dynamics. We therefore evaluated the relationships bet
Adam J. Sepulveda, Robert Al-Chokhachy, Matthew Laramie, Kyle Crapster, Ladd Knotek, Brian T. Miller, Alexander V. Zale, David Pilliod

A round-robin evaluation of the repeatability and reproducibility of environmental DNA assays for dreissenid mussels

Resource managers may be hesitant to make decisions based on environmental (e)DNA results alone since eDNA is an indirect method of species detection. One way to reduce the uncertainty of eDNA is to identify laboratory‐based protocols that ensure repeatable and reproducible results. We conducted a double‐blind round‐robin analysis of probe‐based assays for DNA of dreissenid (Dreissena spp.) mussel
Adam J. Sepulveda, Patrick R. Hutchins, Craig Jackson, Carl Ostberg, Matthew Laramie, Jon Amberg, Timothy Counihan, Andrew B. Hoegh, David Pilliod

Integration of eDNA-based biological monitoring within the US Geological Survey’s national streamgage network

This study explores the feasibility and utility of integrating environmental DNA (eDNA) assessments of species occurrences into the United States (U.S.) Geological Survey’s national streamgage network. We used an existing network of five gages in southwest Idaho to explore the type of information that could be gained as well as the associated costs and limitations. Hydrologic technicians were trai
David Pilliod, Matthew Laramie, Dorene McCoy, Scott Maclean