Improvements in Wastewater Treatment Reduces Endocrine Disruption in Fish

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A team of scientists from the U.S. Geological Survey (USGS), the University of Colorado, and the City of Boulder, Colorado, demonstrated that improvements to the treatment process at a wastewater treatment facility (WWTF) reduced the level of endocrine disruption in fish exposed to wastewater effluent discharged from the facility. It is difficult to anticipate the effects that upgrading WWTF operations might have on stream ecosystems. In a recent article in Environmental Science and Technology the scientists report on a multiyear experiment to evaluate the impact on wastewater quality of a major upgrade to the Boulder, Colorado, WWTF. The facility was converted from a biological filter plant with solids contact to an activated sludge plant.

Fish tanks inside an onsite laboratory

Fish tanks inside an onsite laboratory where fathead minnows were exposed to untreated and treated wastewater for 28 days to test for possible endocrine disruption. Photo credit: Alan Vajda, University of Colorado.

What They Did

In 2005 and 2007 the scientists established the water-quality conditions and level of fish endocrine disruption in test organisms before the upgrade. A similar set of investigations was conducted in 2008 and 2011 to document conditions after the upgrade. The two studies evaluated a wide variety of organic and inorganic contaminants and tracked their concentrations through the various treatment processes. Fish endocrine disruption was measured during controlled, 28-day onsite continuous-flow exposure experiments using different dilutions of wastewater. The fish experiments used sexually mature male fathead minnows and multiple indicators of endocrine disruption (biomarkers).

What They Found

The scientists found that the WWTF upgrade resulted in enhanced removal of hormones (steroidal estrogens) and other endocrine disrupting compounds from the wastewater, as well as a decrease in fish endocrine disruption. This study was funded by the USGS Toxic Substances Hydrology and Hydrologic Research and Development Programs, and the National Science Foundation.

The Boulder Wastewater Treatment Facility, Colorado (circa 2005)

The Boulder Wastewater Treatment Facility, Colorado (circa 2005), before the upgrade to an activated sludge treatment process. Treated effluent from the plant is discharged to nearby Boulder Creek. In a small laboratory, seen in the lower left of the photograph, a team of scientists conducted fish exposure experiments using the treated effluent from the facility. Photo Credit: City of Boulder, Colorado.

References

Barber, L.B., Antweiler, R.C., Flynn, J.L., Keefe, S.H., Kolpin, D.W., Roth, D.A., Schnoebelen, D.J., Taylor, H.E., and Verplanck, P.L., 2011, Lagrangian mass-flow investigations of inorganic contaminants in wastewater-impacted streams: Environmental Science and Technology, v. 45, no. 7, p. 2575-2583, doi:10.1021/es104138y.

Barber, L.B., Brown, G.K., Nettesheim, T.G., Murphy, E.W., Bartell, S.E., and Schoenfuss, H.L., 2011, Effects of biologically-active chemical mixtures on fish in a wastewater-impacted urban stream: Science of the Total Environment, v. 409, no. 22, p. 4720-4728, doi:10.1016/j.scitotenv.2011.06.039.

Barber, L.B., Keefe, S.H., Kolpin, D.W., Schnoebelen, D.J., Flynn, J.L., Brown, G.K., Furlong, E.T., Gray, J.L., Glassmeyer, S.T., Meyer, M.T., Sandstrom, M.W., Taylor, H.E., and Zaugg, S.D., 2011, Lagrangian sampling of wastewater treatment plant discharges into Boulder Creek, Colorado and Fourmile Creek, Iowa during the summer of 2003 and spring of 2005--Hydrological and water-quality data: U.S. Geological Survey Open-File Report 2011-1054, 84 p.

Barber, L.B., Lee, K.E., Swackhamer, D.L., and Schoenfuss, H.L., 2007, Reproductive responses of male fathead minnows exposed to wastewater treatment plant effluent, effluent treated with XAD8 resin, and an environmentally relevant mixture of alkylphenol compounds: Aquatic Toxicology, v. 82, no. 1, p. 36-46, doi:10.1016/j.aquatox.2007.01.003.

Barber, L.B., Murphy, S.F., Verplanck, P.L., Sandstrom, M.W., Taylor, H.E., and Furlong, E.T., 2006, Chemical loading into surface water along a hydrological, biogeochemical, and land use gradient--A holistic watershed approach: Environmental Science and Technology, v. 40, no. 2, p. 475-486, doi:10.1021/es051270q.

Barber, L.B., Vajda, A.M., Douville, C., Norris, D.O., and Writer, J.H., 2012, Fish endocrine disruption responses to a major wastewater treatment facility upgrade: Environmental Science and Technology, v. 46, no. 4, p. 2121-2131, doi:10.1021/es202880e.

Bistodeau, T.J., Barber, L.B., Bartell, S.E., Cediel, R.A., Grove, K.J., Klaustermeier, J., Woodard, J.C., Lee, K.E., and Schoenfuss, H.L., 2006, Larval exposure to environmentally relevant mixtures of alkylphenolethoxylates reduces reproductive competence in male fathead minnows: Aquatic Toxicology, v. 79, p. 268-277, doi:doi:10.1016/j.aquatox.2006.06.015.

Bradley, P.M., 2008, Potential for biodegradation of contaminants of emerging concern in streams systemsin Proceedings of the 2008 South Carolina Water Resources Conference, Charleston, S.C., October 14-15, 2008: (Presentation).

Bradley, P.M., Barber, L.B., Chapelle, F.H., Gray, J.L., Kolpin, D.W., and McMahon, P.B., 2009, Biodegradation of 17β-estradiol, estrone and testosterone in stream sediments: Environmental Science and Technology, v. 43, no. 6, p. 1902-1910, doi:10.1021/es802797j.

Bradley, P.M., Barber, L.B., Kolpin, D.W., McMahon, P.B., and Chapelle, F.H., 2008, Potential for 4-n-nonylphenol biodegradation in stream sediments: Environmental Toxicology and Chemistry, v. 27, no. 2, p. 260-265, doi:10.1897/07-333R.1.

Bradley, P.M., Chapelle, F.H., Barber, L.B., McMahon, P.B., Gray, J.L., and Kolpin, D.W., 2009, Biodegradation of 17β-estradiol, estrone, and testosterone in stream sedimentsin Wickramanayake, G.B., and Rectanus, H.V., eds., In Situ and On-Site Bioremediation-2009--Proceedings of the 10th International In Situ and On-Site Bioremediation Symposium, Baltimore, MD, May 5-8, 2009: Columbus, Ohio, Battelle Memorial Institute, ISBN:9780981973012.

Lozano, N., Rice, C.P., Pagano, J., Zintek, L., Barber, L.B., Murphy, E.W., Nettesheim, T., Minarik, T., and Schoenfuss, H.L., 2012, Concentration of organic contaminants in fish and their biological effects in a wastewater-dominated urban stream: Science of the Total Environment, v. 420, p. 191-201, doi:10.1016/j.scitotenv.2011.12.059.

Murphy, S.F., Barber, L.B., Verplanck, P.L., and Kinner, D.A., 2003, Environmental setting and hydrology of the Boulder Creek Watershed (Chapter 1)in Murphy, S.F., Verplanck, P.L., and Barber, L.B., eds., Comprehensive water quality of the Boulder Creek Watershed, Colorado, during high-flow and low-flow condition, 2000: U.S. Geological Survey Water-Resources Investigations Report 03-4045, p. 5-26.

Murphy, S.F., Verplanck, P.L., and Barber, L.B., 2003, Comprehensive water quality of the Boulder Creek Watershed, Colorado, during high-flow and low-flow conditions, 2000: U.S. Geological Survey Water-Resources Investigations Report 03-4045, 198 p.

Schoenfuss, H.L., Bartell, S.E., Bistodeau, T.B., Cediel, R.A., Grove, K.J., Zintek, L., Lee, K.E., and Barber, L.B., 2008, Impairment of the reproductive potential of male fathead minnows by environmentally relevant exposures to 4-nonylphenol: Aquatic Toxicology, v. 86, no. 1, p. 91-98, doi:10.1016/j.aquatox.2007.10.004.

Schultz, M.M., Furlong, E.T., Kolpin, D.W., Werner, S.L., Schoenfuss, H.L., Barber, L.B., Blazer, V.S., Norris, D.O., and Vajda, A.M., 2010, Antidepressant pharmaceuticals in two U.S. effluent-impacted streams--Occurrence and fate in water and sediment, and selective uptake in fish neural tissue: Environmental Science and Technology, v. 44, no. 6, p. 1,918-1,925, doi:10.1021/es9022706.

Vajda, A.M., Barber, L.B., Gray, J.L., Lopez, E.M., Bolden, A.M., Schoenfuss, H.L., and Norris, D.O., 2011, Demasculinization of male fish by wastewater treatment plant effluent: Aquatic Toxicology, v. 103, no. 3-4, p. 213-221, doi:10.1016/j.aquatox.2011.02.007.

Vajda, A.M., Barber, L.B., Gray, J.L., Lopez, E.M., Woodling, J.D., and Norris, D.O., 2008, Reproductive disruption in fish downstream from an estrogenic wastewater effluent: Environmental Science and Technology, v. 42, no. 9, p. 3,407-3,414, doi:10.1021/es0720661.

Verplanck, P.L., Taylor, H.E., Nordstrom, D.K., and Barber, L.B., 2005, Aqueous stability of gadolinium in surface waters receiving sewage treatment plant effluent, Boulder Creek, Colorado: Environmental Science and Technology, v. 39, no. 18, p. 6923-6929, doi:10.1021/es048456u.

Writer, J.H., Barber, L.B., Brown, G.K., Taylor, H.E., Kiesling, R.L., Ferrey, M.L., Jahns, N.D., Bartell, S.E., and Schoenfuss, H.L., 2010, Anthropogenic tracers, endocrine disrupting chemicals, and endocrine disruption in Minnesota lakes: Science of the Total Environment, v. 409, no. 1, p. 100-111, doi:10.1016/j.scitotenv.2010.07.018.

Writer, J.H., Barber, L.B., Ryan, J.N., and Bradley, P.M., 2011, Biodegradation and attenuation of steroidal hormones and alkylphenols by stream biofilms and sediments: Environmental Science and Technology, v. 45, no. 10, p. 4370-4376, doi:10.1021/es2000134.

Writer, J.H., Ryan, J.N., and Barber, L.B., 2011, Role of biofilms in sorptive removal of steroidal hormones and 4-nonylphenol compounds from streams: Environmental Science and Technology, v. 45, no. 17, p. 7275-7283, doi:10.1021/es2008038.

Writer, J.H., Ryan, J.N., Keefe, S.H., and Barber, L.B., 2012, Fate of 4-nonylphenol and 17β-estradiol in the Redwood River of Minnesota: Environmental Science and Technology, v. 46, no. 2, p. 860-868, doi:10.1021/es2031664.

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