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Woman in full protective gear at a sink in the lab.
RNA Extraction 2
RNA Extraction 2
RNA Extraction 2

Robin Johnson conducts RNA extractions in the Biosafety Level-3 lab at  Leetown, WV

Robin Johnson conducts RNA extractions in the Biosafety Level-3 lab at  Leetown, WV

Woman in a mask holding a sample box.
Frequent Flyer Miles
Frequent Flyer Miles
Frequent Flyer Miles

Rachel Atkins brings collected samples to specific FedEx facilites to ship the precious cargo to West Virginia for processing.

Rachel Atkins brings collected samples to specific FedEx facilites to ship the precious cargo to West Virginia for processing.

Smiling scientist next to a van full of sample boxes.
Prepping boxes for sampling
Prepping boxes for sampling
Prepping boxes for sampling

Rachel Atkins organizes boxes and PPE in preparation for sampling at several facilities in Utah using an oversized cargo van.

Rachel Atkins organizes boxes and PPE in preparation for sampling at several facilities in Utah using an oversized cargo van.

Woman in protective gear placing ice packs into a sample cooler.
Sample Pickup
Sample Pickup
Sample Pickup

Rachel Atkins retrieving and packaging samples from each facility, which must be stored on ice during shipment to the Leetown, WV lab

Rachel Atkins retrieving and packaging samples from each facility, which must be stored on ice during shipment to the Leetown, WV lab

Man in full protective gear working on a sample under the vacuum hood in a lab.
Spike of RHV virus
Spike of RHV virus
Spike of RHV virus

Spike of RHV virus as extraction control in the Biosafety Level-3 Lab. Photos take in the BSL-3 lab are often fuzzy due to phones being kept in sealed plastic for safety.

Spike of RHV virus as extraction control in the Biosafety Level-3 Lab. Photos take in the BSL-3 lab are often fuzzy due to phones being kept in sealed plastic for safety.

Ice packs being placed in a cooler arounda sample red biohazard bag.
Proper Icing
Proper Icing
Proper Icing

Demonstration of how to add ice packs to shipping boxes for proper sample shipping.

Demonstration of how to add ice packs to shipping boxes for proper sample shipping.

Woman in full protective gear entering data into a lab computer.
Logging Samples
Logging Samples
Logging Samples

 Christine Densmore logging newly arrived samples into the BSL-3 laptop to be verified by the data team

 Christine Densmore logging newly arrived samples into the BSL-3 laptop to be verified by the data team

Sample boxed stacked in back of truck.
Delivering Collection Kits to Facilities 1
Delivering Collection Kits to Facilities 1
Delivering Collection Kits to Facilities 1

Rachel Atkins  (UT WSC) distributing sampling kits to the participating wastewater facilities for sample collection in Utah.

Hallway with people adding supplies to sample boxes.
Initial Package Assembly
Initial Package Assembly
Initial Package Assembly

The Logistics Team packs dozens of sample boxes to send to the field teams at multiple Science Centers across the country.

The Logistics Team packs dozens of sample boxes to send to the field teams at multiple Science Centers across the country.

Woman in mask holding a bag with sample bottles.
Wisconsin Sample Collection
Wisconsin Sample Collection
Wisconsin Sample Collection

Laura Hubbard hard at work collecting samples across the state of Wisconsin to send to the Leetown Lab.

Green lab equipment with plastic sample bottles on top.
Zymo iiiP Column
Zymo iiiP Column
Zymo iiiP Column

An example of the setup of the zymo iiiP column and manifold assembly inside the BSL-3 lab. 

An example of the setup of the zymo iiiP column and manifold assembly inside the BSL-3 lab. 

Large warehouse room full of cardboard sample boxes.
Delivered Sample Boxes at the Lab
Delivered Sample Boxes at the Lab
Delivered Sample Boxes at the Lab

Accumulation of post-delivery Sample Boxes in the recieving area of the Leetown facility

Two women carry boxes in a large warehouse room.
Unloading boxes from Fed Ex
Unloading boxes from Fed Ex
Unloading boxes from Fed Ex

Unloading sample boxes from multiple facilities off the Fed Ex Truck, Christine Densmore (front) Robin Johnson (rear) 

Unloading sample boxes from multiple facilities off the Fed Ex Truck, Christine Densmore (front) Robin Johnson (rear) 

Man and Woman in full protective gear work on samples in a lab.
RNA Extractions
RNA Extractions
RNA Extractions

Luke Iwanowicz (left) Christine Densmore (right) perform RNA extractions in the Biosafety Level-3 lab

Luke Iwanowicz (left) Christine Densmore (right) perform RNA extractions in the Biosafety Level-3 lab

Two men in full protective gear work on samples in a lab.
Purification of extracted RNA 1
Purification of extracted RNA 1
Purification of extracted RNA 1

Purification of extracted RNA via vacuum manifold RNA column filtration in the BSL3 lab.

Man in full protective gear carrying bags in a lab.
RNA Extraction 1
RNA Extraction 1
RNA Extraction 1

Luke Iwanowicz conducts RNA extractions in the Biosafety Level-3 lab at  Leetown, WV

Luke Iwanowicz conducts RNA extractions in the Biosafety Level-3 lab at  Leetown, WV

Man and woman in full protective gear work on samples in a lab.
Processing Filtered Samples
Processing Filtered Samples
Processing Filtered Samples

1x filtered samples processed via vacuum manifold RNA column filtration. Photos take in the BSL-3 lab are often fuzzy due to phones being kept in sealed plastic for safety.

1x filtered samples processed via vacuum manifold RNA column filtration. Photos take in the BSL-3 lab are often fuzzy due to phones being kept in sealed plastic for safety.

Man and two women in full protective gear work on samples in a lab.
Transfer or RNA Spin Columns 1
Transfer or RNA Spin Columns 1
Transfer or RNA Spin Columns 1

Transfer of RNA spin columns into collection tubes (with Chris Ottinger and Colleen Young)

Two women in full protective gear work on samples in a lab.
Transfer or RNA Spin Columns 2
Transfer or RNA Spin Columns 2
Transfer or RNA Spin Columns 2

Transfer of RNA spin columns into collection tubes (also pictured Chris Ottinger and Colleen Young)

Woman in full protective gear giving a thumbs-up in a lab.
Deb in the Lab
Deb in the Lab
Deb in the Lab

Deb Iwanowicz grabs a rare selfie from a plastic-encased phone the BSL3 lab.          

Deb Iwanowicz grabs a rare selfie from a plastic-encased phone the BSL3 lab.          

Woman in protective gear giving a thumbs-up a wastewater facility.
Sampling is FUN
Sampling is FUN
Sampling is FUN

Chelsea Delsack snaps a selfie in sample pickup PPE after packaging samples for shipment to Leetown, WV

Chelsea Delsack snaps a selfie in sample pickup PPE after packaging samples for shipment to Leetown, WV

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