Cathy Richter, PhD
Dr. Cathy Richter is a Research Molecular Biologist at the Columbia Environmental Research Center.
Science and Products
Filter Total Items: 19
Polyacrylamide gel electrophoresis results with thiaminase activity stain of recombinant putative thiaminases expressed in E. coli
Data describe results of a set of laboratory experiments using polyacrylamide gel electrophoresis with a thiaminase activity stain to test the solubility and thiaminase activity of recombinant putative thiaminases expressed in E. coli. Parameters described include the presence and relative mobility of thiaminases, and the presence and relative mobility of recombinant proteins. Experiments were con
Longnose darter (Percina nasuta) eDNA survey results from the St. Francis River, Missouri 2018
Data describe a designed environmental DNA (eDNA) survey for the detection of longnose darter (Percina nasuta) in field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater fish species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect th
Metabarcoding data from freshwater mussel eDNA collected in Clinch River, Virginia 2017
Data from metabarcoding assays to detect a suite of mussel species using mitochondrial DNA regions of the cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit (ND1) genes sequences.
Mucket eDNA detection in Wallen's Bend, Clinch river, Tennessee, September 2019
The data describe the technical performance of a designed qPCR assay for the detection of mucket (Actinonaias ligamentina) eDNA from field collected water samples. Parameters described include the sequences of the primers and probes used; the limit of detection and limit of quantification for the assay; a list of freshwater mussels species that were used to test specificity of the assay; and in si
Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17a-ethinylestradiol)
Largemouth base histological development and transcriptomic changes in gonad tissue after early life stage exposure to Atrazine (1-Chloro-3-ethylamino-5-isopropylamino-2,4,6-triazine) or the model estrogen 17a-ethinylestradiol.
Asian carp eDNA and egg morphology data collected from Truman Reservoir tributaries, Missouri, USA, 2014
Ichthyoplankton tows samples collected from Truman reservoir tributaries evaluated for eDNA. Data also includes hydrologic and water quality parameters and morphology of grass carp eggs.
eDNA sampling sites in the Grand Canyon region near breccia pipe uranium mines_2015_2016
Geospatial locations of eDNA sampling sites in the Grand Canyon region near breccia pipe uranium mines
Filter Total Items: 44
A comparison of eDNA and visual survey methods for detection of longnose darter Percina nasuta in Missouri
The longnose darter Percina nasuta is a rare and cryptic fish that recently disappeared from much of its historic range. We developed and used an environmental DNA (eDNA) assay for longnose darter paired with visual surveys to better determine the species’ range and compare detection probability between sampling approaches in an occupancy modeling framework. We detected longnose darter eDNA furthe
Authors
Jacob Thomas Westhoff, Leah K. Berkman, Katy E. Klymus, Nathan Thompson, Cathy A. Richter
Reproduction
Lake charr Salvelinus namaycush are typically fall spawners although one ecotype has populations that spawn during spring and fall (siscowets in Lake Superior). Lake charr are iteroparous (reproduce more than once in a lifetime) with group-synchronous ovarian development and typically spawn once per year. However, lake charr may not reproduce every year, a phenomenon known as skipped spawning. Fre
Authors
Frederick W. Goetz, J. Ellen Marsden, Catherine A. Richter, Donald E. Tillitt, Shawn P. Sitar, Stephen Riley, Charles C. Krueger
Metabarcoding assays for the detection of freshwater mussels (Unionida) with environmental DNA
Freshwater mussels of the order Unionida are a widely distributed taxon that are important in maintaining freshwater ecosystems and are also highly imperiled throughout the world. Monitoring of mussel populations with environmental DNA (eDNA) is an attractive alternative to traditional methods because it is noninvasive and requires less labor and taxonomic knowledge from field personnel. We develo
Authors
Katy E. Klymus, Catherine A. Richter, Nathan Thompson, Jo Ellen Hinck, Jess W. Jones
Development and testing of species-specific quantitative PCR assays for environmental DNA applications
New, non-invasive methods for detecting and monitoring species presence are being developed to aid in fisheries and wildlife conservation management. The use of environmental DNA (eDNA) samples for detecting macrobiota is one such group of methods that is rapidly becoming popular and being implemented in national management programs. Here we focus on the development of species-specific targeted as
Authors
Katy E. Klymus, Dannise Vannesa Ruiz Ramos, Nathan Thompson, Catherine A. Richter
Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17α-ethinylestradiol)
Endocrine disrupting contaminants are of continuing concern for potentially contributing to reproductive dysfunction in largemouth and smallmouth bass in the Chesapeake Bay watershed (CBW) and elsewhere. Exposures to atrazine (ATR) have been hypothesized to have estrogenic effects on vertebrate endocrine systems. The incidence of intersex in male smallmouth bass from some regions of CBW has been c
Authors
Jessica Kristin Leet, Catherine A. Richter, Robert S. Cornman, Jason P. Berninger, Ramji K. Bhandari, Diane K. Nicks, James L. Zajicek, Vicki S. Blazer, Donald E. Tillitt
Use of environmental DNA to detect grass carp spawning events
The timing and location of spawning events are important data for managers seeking to control invasive grass carp populations. Ichthyoplankton tows for grass carp eggs and larvae can be used to detect spawning events; however, these samples can be highly debris-laden, and are expensive and laborious to process. An alternative method, environmental DNA (eDNA) technology, has proven effective in det
Authors
Cari-Ann Hayer, Michael F. Bayless, Amy E. George, Nathan Thompson, Catherine A. Richter, Duane Chapman
Reporting the limits of detection and quantification for environmental DNA assays
BackgroundEnvironmental DNA (eDNA) analysis is increasingly being used to detect the presence and relative abundance of rare species, especially invasive or imperiled aquatic species. The rapid progress in the eDNA field has resulted in numerous studies impacting conservation and management actions. However, standardization of eDNA methods and reporting across the field is yet to be fully establis
Authors
Katy E. Klymus, Christopher M. Merkes, Michael J. Allison, Caren S. Goldberg, Caren C. Helbing, Margaret Hunter, Craig Jackson, Richard F. Lance, Anna M. Mangan, Emy M. Monroe, Antoinette J. Piaggio, Joel P. Stokdyk, Chris C. Wilson, Catherine A. Richter
Thiamine deficiency in fishes: Causes, consequences, and potential solutions
Thiamine deficiency complex (TDC) is a disorder resulting from the inability to acquire or retain thiamine (vitamin B1) and has been documented in organisms in aquatic ecosystems ranging from the Baltic Sea to the Laurentian Great Lakes. The biological mechanisms leading to TDC emergence may vary among systems, but in fishes, one common outcome is high mortality among early life stages. Here, we r
Authors
Avril M. Harder, William R. Ardren, Allison N. Evans, Matthew H. Futia, Clifford E. Kraft, J. Ellen Marsden, Catherine A. Richter, Jacques Rinchard, Donald E. Tillitt, Mark R. Christie
Metabarcoding of environmental DNA samples to explore the use of uranium mine containment ponds as a water source for wildlife
Understanding how anthropogenic impacts on the landscape affect wildlife requires a knowledge of community assemblages. Species surveys are the first step in assessing community structure, and recent molecular applications such as metabarcoding and environmental DNA analyses have been proposed as an additional and complementary wildlife survey method. Here, we test eDNA metabarcoding as a survey t
Authors
Katy E. Klymus, Catherine A. Richter, Nathan Thompson, Jo Ellen Hinck
Experimental observations on the decay of environmental DNA from bighead and silver carps
Interest in the field of environmental DNA (eDNA) is growing rapidly and eDNA surveys are becoming an important consideration for aquatic resource managers dealing with invasive species. However, in order for eDNA monitoring to mature as a research and management tool, there are several critical knowledge gaps that must be filled. One such gap is the fate of eDNA materials in the aquatic environme
Authors
Richard F. Lance, Katy E. Klymus, Catherine A. Richter, Xin Guan, Heather L. Farrington, Matthew R. Carr, Nathan Thompson, Duane Chapman, Kelly L. Baerwaldt
Evaluation of potential mechanisms of atrazine-induced reproductive impairment in fathead minnow (Pimephales promelas) and Japanese medaka (Oryzias latipes)
Atrazine has been implicated in reproductive dysfunction of exposed organisms, and previous studies documented decreased egg production in Japanese medaka (Oryzias latipes) and fathead minnows (Pimephales promelas) during 30-d to 38-d exposures to 0.5 µg/L, 5 µg/L, and 50 µg/L atrazine. The authors evaluated possible mechanisms underlying the reduction in egg production. Gene expression in steroid
Authors
Catherine A. Richter, Diana M. Papoulias, Jeffrey J. Whyte, Donald E. Tillitt
First direct confirmation of grass carp spawning in a Great Lakes tributary
Grass carp (Ctenopharyngodon idella), an invasive species of Asian carp, has been stocked for many decades in the United States for vegetation control. Adult individuals have been found in all of the Great Lakes except Lake Superior, but no self-sustaining populations have yet been identified in Great Lakes tributaries. In 2012, a commercial fisherman caught four juvenile diploid grass carp in the
Authors
Holly S. Embke, Patrick M. Kočovský, Cathy A. Richter, Jeremy J. Pritt, Christine M. Mayer, Song Qian
Non-USGS Publications**
Taylor, J.A., Richter, C.A., Suzuki, A., Watanabe, H., Iguchi, T., Coser, K.R., Shioda, T., and vom Saal, F.S., 2012, Dose-related estrogen effects on gene expression in fetal mouse prostate mesenchymal cells: PLOS ONE, v. 7, no. 10, p. e48311
Taylor, J.A., Richter, C.A., Ruhlen, R.L., and vom Saal, F.S., 2011, Estrogenic environmental chemicals and drugs: Mechanisms for effects on the developing male urogenital system: The Journal of Steroid Biochemistry and Molecular Biology, v. 127, no. 1, p. 83-95
Richter, C.A., Taylor, J.A., Ruhlen, R.L., Welshons, W.V., and vom Saal, F.S., 2007, Estradiol and Bisphenol A stimulate androgen receptor and estrogen receptor gene expression in fetal mouse prostate mesenchyme cells: Environmental Health Perspectives, v. 115, no. 6, p. 902-908
vom Saal, F.S., Richter, C.A., Mao, J., and Welshons, W.V., 2005, Commercial animal feed: Variability in estrogenic activity and effects on body weight in mice: Birth Defects Research Part A: Clinical and Molecular Teratology, v. 73, no. 7, p. 474-475
Timms, B.G., Howdeshell, K.L., Barton, L., Bradley, S., Richter, C.A., and vom Saal, F.S., 2005, Estrogenic chemicals in plastic and oral contraceptives disrupt development of the fetal mouse prostate and urethra: Proceedings of the National Academy of Sciences of the United States of America, v. 102, no. 19, p. 7014-7019
Richter, C., Timms, B., and vom Saal, F., 2005, Prostate development: mechanisms for opposite effects of low and high doses of estrogenic chemicals, in Naz, R., ed., Endocrine Disruptors: Effects on Male and Female Reproductive Systems: New York, CRC Press, p. 379-410.
vom Saal, F.S., Richter, C.A., Ruhlen, R.R., Nagel, S.C., Timms, B.G., and Welshons, W.V., 2005, The importance of appropriate controls, animal feed, and animal models in interpreting results from low-dose studies of bisphenol A: Birth Defects Research Part A: Clinical and Molecular Teratology, v. 73, no. 3, p. 140-145
vom Saal, F.S., Richter, C.A., Ruhlen, R.R., Nagel, S.C., and Welshons, W.V., 2004, Disruption of laboratory experiments due to leaching of Bisphenol A from polycarbonate cages and bottles and uncontrolled variability in components of animal feed, in National Research Council, ed., The Development of Science-based Guidelines for Laboratory Animal Care: Proceedings of the November 2003 International Workshop: The National Academies Press, p. 65-69.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Science and Products
Filter Total Items: 19
Polyacrylamide gel electrophoresis results with thiaminase activity stain of recombinant putative thiaminases expressed in E. coli
Data describe results of a set of laboratory experiments using polyacrylamide gel electrophoresis with a thiaminase activity stain to test the solubility and thiaminase activity of recombinant putative thiaminases expressed in E. coli. Parameters described include the presence and relative mobility of thiaminases, and the presence and relative mobility of recombinant proteins. Experiments were con
Longnose darter (Percina nasuta) eDNA survey results from the St. Francis River, Missouri 2018
Data describe a designed environmental DNA (eDNA) survey for the detection of longnose darter (Percina nasuta) in field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater fish species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect th
Metabarcoding data from freshwater mussel eDNA collected in Clinch River, Virginia 2017
Data from metabarcoding assays to detect a suite of mussel species using mitochondrial DNA regions of the cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit (ND1) genes sequences.
Mucket eDNA detection in Wallen's Bend, Clinch river, Tennessee, September 2019
The data describe the technical performance of a designed qPCR assay for the detection of mucket (Actinonaias ligamentina) eDNA from field collected water samples. Parameters described include the sequences of the primers and probes used; the limit of detection and limit of quantification for the assay; a list of freshwater mussels species that were used to test specificity of the assay; and in si
Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17a-ethinylestradiol)
Largemouth base histological development and transcriptomic changes in gonad tissue after early life stage exposure to Atrazine (1-Chloro-3-ethylamino-5-isopropylamino-2,4,6-triazine) or the model estrogen 17a-ethinylestradiol.
Asian carp eDNA and egg morphology data collected from Truman Reservoir tributaries, Missouri, USA, 2014
Ichthyoplankton tows samples collected from Truman reservoir tributaries evaluated for eDNA. Data also includes hydrologic and water quality parameters and morphology of grass carp eggs.
eDNA sampling sites in the Grand Canyon region near breccia pipe uranium mines_2015_2016
Geospatial locations of eDNA sampling sites in the Grand Canyon region near breccia pipe uranium mines
Filter Total Items: 44
A comparison of eDNA and visual survey methods for detection of longnose darter Percina nasuta in Missouri
The longnose darter Percina nasuta is a rare and cryptic fish that recently disappeared from much of its historic range. We developed and used an environmental DNA (eDNA) assay for longnose darter paired with visual surveys to better determine the species’ range and compare detection probability between sampling approaches in an occupancy modeling framework. We detected longnose darter eDNA furthe
Authors
Jacob Thomas Westhoff, Leah K. Berkman, Katy E. Klymus, Nathan Thompson, Cathy A. Richter
Reproduction
Lake charr Salvelinus namaycush are typically fall spawners although one ecotype has populations that spawn during spring and fall (siscowets in Lake Superior). Lake charr are iteroparous (reproduce more than once in a lifetime) with group-synchronous ovarian development and typically spawn once per year. However, lake charr may not reproduce every year, a phenomenon known as skipped spawning. Fre
Authors
Frederick W. Goetz, J. Ellen Marsden, Catherine A. Richter, Donald E. Tillitt, Shawn P. Sitar, Stephen Riley, Charles C. Krueger
Metabarcoding assays for the detection of freshwater mussels (Unionida) with environmental DNA
Freshwater mussels of the order Unionida are a widely distributed taxon that are important in maintaining freshwater ecosystems and are also highly imperiled throughout the world. Monitoring of mussel populations with environmental DNA (eDNA) is an attractive alternative to traditional methods because it is noninvasive and requires less labor and taxonomic knowledge from field personnel. We develo
Authors
Katy E. Klymus, Catherine A. Richter, Nathan Thompson, Jo Ellen Hinck, Jess W. Jones
Development and testing of species-specific quantitative PCR assays for environmental DNA applications
New, non-invasive methods for detecting and monitoring species presence are being developed to aid in fisheries and wildlife conservation management. The use of environmental DNA (eDNA) samples for detecting macrobiota is one such group of methods that is rapidly becoming popular and being implemented in national management programs. Here we focus on the development of species-specific targeted as
Authors
Katy E. Klymus, Dannise Vannesa Ruiz Ramos, Nathan Thompson, Catherine A. Richter
Effects of early life stage exposure of largemouth bass to atrazine or a model estrogen (17α-ethinylestradiol)
Endocrine disrupting contaminants are of continuing concern for potentially contributing to reproductive dysfunction in largemouth and smallmouth bass in the Chesapeake Bay watershed (CBW) and elsewhere. Exposures to atrazine (ATR) have been hypothesized to have estrogenic effects on vertebrate endocrine systems. The incidence of intersex in male smallmouth bass from some regions of CBW has been c
Authors
Jessica Kristin Leet, Catherine A. Richter, Robert S. Cornman, Jason P. Berninger, Ramji K. Bhandari, Diane K. Nicks, James L. Zajicek, Vicki S. Blazer, Donald E. Tillitt
Use of environmental DNA to detect grass carp spawning events
The timing and location of spawning events are important data for managers seeking to control invasive grass carp populations. Ichthyoplankton tows for grass carp eggs and larvae can be used to detect spawning events; however, these samples can be highly debris-laden, and are expensive and laborious to process. An alternative method, environmental DNA (eDNA) technology, has proven effective in det
Authors
Cari-Ann Hayer, Michael F. Bayless, Amy E. George, Nathan Thompson, Catherine A. Richter, Duane Chapman
Reporting the limits of detection and quantification for environmental DNA assays
BackgroundEnvironmental DNA (eDNA) analysis is increasingly being used to detect the presence and relative abundance of rare species, especially invasive or imperiled aquatic species. The rapid progress in the eDNA field has resulted in numerous studies impacting conservation and management actions. However, standardization of eDNA methods and reporting across the field is yet to be fully establis
Authors
Katy E. Klymus, Christopher M. Merkes, Michael J. Allison, Caren S. Goldberg, Caren C. Helbing, Margaret Hunter, Craig Jackson, Richard F. Lance, Anna M. Mangan, Emy M. Monroe, Antoinette J. Piaggio, Joel P. Stokdyk, Chris C. Wilson, Catherine A. Richter
Thiamine deficiency in fishes: Causes, consequences, and potential solutions
Thiamine deficiency complex (TDC) is a disorder resulting from the inability to acquire or retain thiamine (vitamin B1) and has been documented in organisms in aquatic ecosystems ranging from the Baltic Sea to the Laurentian Great Lakes. The biological mechanisms leading to TDC emergence may vary among systems, but in fishes, one common outcome is high mortality among early life stages. Here, we r
Authors
Avril M. Harder, William R. Ardren, Allison N. Evans, Matthew H. Futia, Clifford E. Kraft, J. Ellen Marsden, Catherine A. Richter, Jacques Rinchard, Donald E. Tillitt, Mark R. Christie
Metabarcoding of environmental DNA samples to explore the use of uranium mine containment ponds as a water source for wildlife
Understanding how anthropogenic impacts on the landscape affect wildlife requires a knowledge of community assemblages. Species surveys are the first step in assessing community structure, and recent molecular applications such as metabarcoding and environmental DNA analyses have been proposed as an additional and complementary wildlife survey method. Here, we test eDNA metabarcoding as a survey t
Authors
Katy E. Klymus, Catherine A. Richter, Nathan Thompson, Jo Ellen Hinck
Experimental observations on the decay of environmental DNA from bighead and silver carps
Interest in the field of environmental DNA (eDNA) is growing rapidly and eDNA surveys are becoming an important consideration for aquatic resource managers dealing with invasive species. However, in order for eDNA monitoring to mature as a research and management tool, there are several critical knowledge gaps that must be filled. One such gap is the fate of eDNA materials in the aquatic environme
Authors
Richard F. Lance, Katy E. Klymus, Catherine A. Richter, Xin Guan, Heather L. Farrington, Matthew R. Carr, Nathan Thompson, Duane Chapman, Kelly L. Baerwaldt
Evaluation of potential mechanisms of atrazine-induced reproductive impairment in fathead minnow (Pimephales promelas) and Japanese medaka (Oryzias latipes)
Atrazine has been implicated in reproductive dysfunction of exposed organisms, and previous studies documented decreased egg production in Japanese medaka (Oryzias latipes) and fathead minnows (Pimephales promelas) during 30-d to 38-d exposures to 0.5 µg/L, 5 µg/L, and 50 µg/L atrazine. The authors evaluated possible mechanisms underlying the reduction in egg production. Gene expression in steroid
Authors
Catherine A. Richter, Diana M. Papoulias, Jeffrey J. Whyte, Donald E. Tillitt
First direct confirmation of grass carp spawning in a Great Lakes tributary
Grass carp (Ctenopharyngodon idella), an invasive species of Asian carp, has been stocked for many decades in the United States for vegetation control. Adult individuals have been found in all of the Great Lakes except Lake Superior, but no self-sustaining populations have yet been identified in Great Lakes tributaries. In 2012, a commercial fisherman caught four juvenile diploid grass carp in the
Authors
Holly S. Embke, Patrick M. Kočovský, Cathy A. Richter, Jeremy J. Pritt, Christine M. Mayer, Song Qian
Non-USGS Publications**
Taylor, J.A., Richter, C.A., Suzuki, A., Watanabe, H., Iguchi, T., Coser, K.R., Shioda, T., and vom Saal, F.S., 2012, Dose-related estrogen effects on gene expression in fetal mouse prostate mesenchymal cells: PLOS ONE, v. 7, no. 10, p. e48311
Taylor, J.A., Richter, C.A., Ruhlen, R.L., and vom Saal, F.S., 2011, Estrogenic environmental chemicals and drugs: Mechanisms for effects on the developing male urogenital system: The Journal of Steroid Biochemistry and Molecular Biology, v. 127, no. 1, p. 83-95
Richter, C.A., Taylor, J.A., Ruhlen, R.L., Welshons, W.V., and vom Saal, F.S., 2007, Estradiol and Bisphenol A stimulate androgen receptor and estrogen receptor gene expression in fetal mouse prostate mesenchyme cells: Environmental Health Perspectives, v. 115, no. 6, p. 902-908
vom Saal, F.S., Richter, C.A., Mao, J., and Welshons, W.V., 2005, Commercial animal feed: Variability in estrogenic activity and effects on body weight in mice: Birth Defects Research Part A: Clinical and Molecular Teratology, v. 73, no. 7, p. 474-475
Timms, B.G., Howdeshell, K.L., Barton, L., Bradley, S., Richter, C.A., and vom Saal, F.S., 2005, Estrogenic chemicals in plastic and oral contraceptives disrupt development of the fetal mouse prostate and urethra: Proceedings of the National Academy of Sciences of the United States of America, v. 102, no. 19, p. 7014-7019
Richter, C., Timms, B., and vom Saal, F., 2005, Prostate development: mechanisms for opposite effects of low and high doses of estrogenic chemicals, in Naz, R., ed., Endocrine Disruptors: Effects on Male and Female Reproductive Systems: New York, CRC Press, p. 379-410.
vom Saal, F.S., Richter, C.A., Ruhlen, R.R., Nagel, S.C., Timms, B.G., and Welshons, W.V., 2005, The importance of appropriate controls, animal feed, and animal models in interpreting results from low-dose studies of bisphenol A: Birth Defects Research Part A: Clinical and Molecular Teratology, v. 73, no. 3, p. 140-145
vom Saal, F.S., Richter, C.A., Ruhlen, R.R., Nagel, S.C., and Welshons, W.V., 2004, Disruption of laboratory experiments due to leaching of Bisphenol A from polycarbonate cages and bottles and uncontrolled variability in components of animal feed, in National Research Council, ed., The Development of Science-based Guidelines for Laboratory Animal Care: Proceedings of the November 2003 International Workshop: The National Academies Press, p. 65-69.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.