Jon Amberg
Science and Products
Developing RNA Interference to Control Zebra Mussels
We aim to develop a control tool for eliminating zebra mussels that exploits natural gene regulation mechanisms (RNA-induced gene silencing; RNAi) to be specific to zebra mussels without non-target effects. In addition to the nuisance they pose, Pimentel et al. estimated that zebra mussels cost the U.S. economy over $1 billion annually. Zebra mussels are among the International Union for...
BOR environmental DNA sampling for invasive mussels at USGS gages
As part of an ongoing project funded by the Bureau of Reclamation and lead by the Idaho Water Sciences Center (IDWSC) and Northern Rocky Mountain Research Center (NOROCK), the Upper Midwest Environmental Sciences Center (UMESC) will analyze environmental DNA samples collected at gage stations directly downstream of multiple reservoirs throughout the Columbia River Basin. The goal of this project...
Field Evaluation of Microparticles
With this study, we will validate the use of microparticles to selectively deliver a control agent to feral bigheaded carps. Prior controlled studies conducted in ponds at the Columbia Environmental Research Center (CERC) in Columbia, Missouri indicated that microparticles could be used to deliver antimycin to bigheaded carps while not impacting non-target species like Largemouth Bass and Grass...
Flocks of a feather dock together: Using Docker and HTCondor to link high-throughput computing across the USGS
USGS scientists often face computationally intensive tasks that require high-throughput computing capabilities. Several USGS facilities use HTCondor to run their computational pools but are not necessarily connected to the larger USGS pool. This project demonstrated how to connect HTCondor pools by flocking, or coordinating, within the USGS. In addition to flocking the Upper Midwest Environmental
Increasing Efficiency and Throughput of eDNA
The results from this study will provide protocols for the collection, processing and analysis of environmental deoxyribonucleic acids (eDNA) samples for monitoring aquatic invasive species like Asian carp. These protocols have the potential to be used by fishery management agencies to monitor aquatic invasive species throughout the United States.
Use of antimycin-microparticles and corn feed to control Common Carp Cyprinus carpio
Attempts to control carp, Cyprinus carpio, populations in Minnesota (and elsewhere) date back to early 1900s (Moyle et al. 1950, Moyle and Kuehn 1964). Early efforts focused on removing large numbers of adult carp, often by targeting their winter aggregations using seine nets. Such was the strategy in Minnesota in 1930s and 40s, where carp were systematically removed with nets in dozens of lakes...
Correlating seasonal trends and occupancy of bigheaded carp eDNA to land use and stream characteristics
The results of this study will advance our understanding of environmental DNA (eDNA) and how eDNA signatures change as fish behavior changes over the course of the open water season. This will further develop eDNA as an easy sampling method to collect information about invading populations or cryptic species that are difficult to capture so that resource managers can be given better information to...
Relationship between fish presence and abundance in Arizona standing waters measured through standard techniques and environmental DNA
The unpredictability of DNA degradation in the environment has plagued the ability of scientists to interpret environmental DNA (eDNA) data since the technique has been used for early detection of aquatic invasive species (AIS) such as silver and bighead carps (Hypophthalmichthys molitrix and Hypophthalmichthys nobilis) throughout the Chicago Area Waterway System (CAWS), Des Plaines River, near...
Assessing the properties of RNA degradation in complex environmental water samples
The unpredictability of nucleic acid degradation in the environment has plagued the ability of scientists to interpret environmental DNA (eDNA) data since the technique has been used for early detection of aquatic invasive species (AIS) such as silver and bighead carps (Hypophthalmichthys molitrix and Hypophthalmichthys nobilis) throughout the Chicago Area Waterway System (CAWS), Des Plaines River...
Use of antimycin-microparticles and alfalfa to control Grass Carp Ctenopharyngodon idella
Since their introduction in the 1960’s, grass carp (Ctenopharyngodon idella) have spread across the North American continent as far north as the U.S./Canadian border in the Great Lakes. Grass carp are an herbivorous species and can significantly reduce the amount of macrophytes and other plant material in a body of water. Subsequently, this can lead to many detrimental effects for certain species...
Hunting Invasive Species with HTCondor: High Throughput Computing for Big Data and Next Generation Sequencing
Large amounts of data are being generated that require hours, days, or even weeks to analyze using traditional computing resources. Innovative solutions must be implemented to analyze the data in a reasonable timeframe. The program HTCondor (https://research.cs.wisc.edu/htcondor/) takes advantage of the processing capacity of individual desktop computers and dedicated computing resources as a sing
Science and Products
- Publications
- Science
Developing RNA Interference to Control Zebra Mussels
We aim to develop a control tool for eliminating zebra mussels that exploits natural gene regulation mechanisms (RNA-induced gene silencing; RNAi) to be specific to zebra mussels without non-target effects. In addition to the nuisance they pose, Pimentel et al. estimated that zebra mussels cost the U.S. economy over $1 billion annually. Zebra mussels are among the International Union for...BOR environmental DNA sampling for invasive mussels at USGS gages
As part of an ongoing project funded by the Bureau of Reclamation and lead by the Idaho Water Sciences Center (IDWSC) and Northern Rocky Mountain Research Center (NOROCK), the Upper Midwest Environmental Sciences Center (UMESC) will analyze environmental DNA samples collected at gage stations directly downstream of multiple reservoirs throughout the Columbia River Basin. The goal of this project...Field Evaluation of Microparticles
With this study, we will validate the use of microparticles to selectively deliver a control agent to feral bigheaded carps. Prior controlled studies conducted in ponds at the Columbia Environmental Research Center (CERC) in Columbia, Missouri indicated that microparticles could be used to deliver antimycin to bigheaded carps while not impacting non-target species like Largemouth Bass and Grass...Flocks of a feather dock together: Using Docker and HTCondor to link high-throughput computing across the USGS
USGS scientists often face computationally intensive tasks that require high-throughput computing capabilities. Several USGS facilities use HTCondor to run their computational pools but are not necessarily connected to the larger USGS pool. This project demonstrated how to connect HTCondor pools by flocking, or coordinating, within the USGS. In addition to flocking the Upper Midwest EnvironmentalIncreasing Efficiency and Throughput of eDNA
The results from this study will provide protocols for the collection, processing and analysis of environmental deoxyribonucleic acids (eDNA) samples for monitoring aquatic invasive species like Asian carp. These protocols have the potential to be used by fishery management agencies to monitor aquatic invasive species throughout the United States.Use of antimycin-microparticles and corn feed to control Common Carp Cyprinus carpio
Attempts to control carp, Cyprinus carpio, populations in Minnesota (and elsewhere) date back to early 1900s (Moyle et al. 1950, Moyle and Kuehn 1964). Early efforts focused on removing large numbers of adult carp, often by targeting their winter aggregations using seine nets. Such was the strategy in Minnesota in 1930s and 40s, where carp were systematically removed with nets in dozens of lakes...Correlating seasonal trends and occupancy of bigheaded carp eDNA to land use and stream characteristics
The results of this study will advance our understanding of environmental DNA (eDNA) and how eDNA signatures change as fish behavior changes over the course of the open water season. This will further develop eDNA as an easy sampling method to collect information about invading populations or cryptic species that are difficult to capture so that resource managers can be given better information to...Relationship between fish presence and abundance in Arizona standing waters measured through standard techniques and environmental DNA
The unpredictability of DNA degradation in the environment has plagued the ability of scientists to interpret environmental DNA (eDNA) data since the technique has been used for early detection of aquatic invasive species (AIS) such as silver and bighead carps (Hypophthalmichthys molitrix and Hypophthalmichthys nobilis) throughout the Chicago Area Waterway System (CAWS), Des Plaines River, near...Assessing the properties of RNA degradation in complex environmental water samples
The unpredictability of nucleic acid degradation in the environment has plagued the ability of scientists to interpret environmental DNA (eDNA) data since the technique has been used for early detection of aquatic invasive species (AIS) such as silver and bighead carps (Hypophthalmichthys molitrix and Hypophthalmichthys nobilis) throughout the Chicago Area Waterway System (CAWS), Des Plaines River...Use of antimycin-microparticles and alfalfa to control Grass Carp Ctenopharyngodon idella
Since their introduction in the 1960’s, grass carp (Ctenopharyngodon idella) have spread across the North American continent as far north as the U.S./Canadian border in the Great Lakes. Grass carp are an herbivorous species and can significantly reduce the amount of macrophytes and other plant material in a body of water. Subsequently, this can lead to many detrimental effects for certain species...Hunting Invasive Species with HTCondor: High Throughput Computing for Big Data and Next Generation Sequencing
Large amounts of data are being generated that require hours, days, or even weeks to analyze using traditional computing resources. Innovative solutions must be implemented to analyze the data in a reasonable timeframe. The program HTCondor (https://research.cs.wisc.edu/htcondor/) takes advantage of the processing capacity of individual desktop computers and dedicated computing resources as a sing - Data
- News
- Multimedia