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Robert Gale, PhD (Former Employee)

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Huron River at Milan, OH

Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

An effects-directed analyses (EDA) framework for endocrine active chemicals was developed to help decision makers quickly evaluate the efficacy and practicality of an EDA approach in waste and surface waters and how adjustments could be made to increase its success.
By
Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology
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Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

An effects-directed analyses (EDA) framework for endocrine active chemicals was developed to help decision makers quickly evaluate the efficacy and practicality of an EDA approach in waste and surface waters and how adjustments could be made to increase its success.
Learn More
link
Huron River at Milan, OH

Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

An effects-directed analyses (EDA) framework for endocrine active chemicals was developed to help decision makers quickly evaluate the efficacy and practicality of an EDA approach in waste and surface waters and how adjustments could be made to increase its success.
By
Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology
link

Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

An effects-directed analyses (EDA) framework for endocrine active chemicals was developed to help decision makers quickly evaluate the efficacy and practicality of an EDA approach in waste and surface waters and how adjustments could be made to increase its success.
Learn More
link
Huron River at Milan, OH

Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

An effects-directed analyses (EDA) framework for endocrine active chemicals was developed to help decision makers quickly evaluate the efficacy and practicality of an EDA approach in waste and surface waters and how adjustments could be made to increase its success.
By
Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology
link

Framework Developed to Evaluate the Practicality of Effects-Directed Analyses to Identify Endocrine Active Chemicals in Complex Environmental Sample Mixtures

An effects-directed analyses (EDA) framework for endocrine active chemicals was developed to help decision makers quickly evaluate the efficacy and practicality of an EDA approach in waste and surface waters and how adjustments could be made to increase its success.
Learn More

Quantitation of estrogens in water, alone and in combination with atrazine, using two chemically-activated luciferase expression, CALUX, bioassays and quadrupole-time of flight ultraperformance liquid chromatography/mass spectrometry, UPLC-MS

This data describes the relative luminescence units and relative fluorescence units of lysates of two estrogen responsive chemically-activated luciferase expression human breast VM7Luc4E2 and VM7LucERbc9 cells which were exposed for 24 hours to estrone and 17beta-estradiol dilutions alone and in mixture with atrazine or water extracts collected from dilutor tanks during a fish exposure study utili
By
Columbia Environmental Research Center

Water chemistry and fish metrics data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture

Physiological endpoints observed in adult largemouth bass in response to an EE2 (17 alpha-ethinylestradiol) or mixture of estrone and atrazine exposure under laboratory pond conditions. Also included are water quality and chemical concentration data. Adult LMB were exposed in outdoor pond mesocosms from post-spawning to early gonad recrudescence to either EE2 (2.4 nanograms per liter), or a mixtur
By
Columbia Environmental Research Center

Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions.

Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Also included are water quality and chemical concentration data.
By
Columbia Environmental Research Center, Western Fisheries Research Center, Fish Health Program (FHP)

Acute toxicity and clotting times of anticoagulant rodenticides to red-toothed (Odonus niger) and black (Melichthys niger) triggerfish, fathead minnow (Pimephales promelas), and largemouth bass (Micropterus salmoides)

Acute toxicity and clotting times of anticoagulant rodenticides of four fish species
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Columbia Environmental Research Center
Filter Total Items: 40

Comparison of two estrogen chemically activated luciferase expression cell bioassays to liquid chromatography–mass spectrometry for quantifying estrone in water samples

Chemically activated luciferase expression (CALUX) cell bioassays are popular tools for assessing endocrine activity of chemicals such as certain environmental contaminants. Although activity equivalents can be obtained from CALUX analysis, directly comparing these equivalents to those obtained from analytical chemistry methods can be problematic because of the complexity of endocrine active pathw
Authors
Jennifer C. Brennan, Abigail Henke, Robert Gale, Diane K. Nicks, Donald Tillitt
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Ecosystems Mission Area, Columbia Environmental Research Center

Declines in reproductive condition of male largemouth bass (Micropterus salmoides) following seasonal exposure to estrogenic endocrine-disrupting compounds

Reproductive abnormalities, that could lead to possible effects at the population level, have been observed in wild fish throughout the United States, with high prevalence in largemouth bass (LMB; Micropterus salmoides) and smallmouth bass (Micropterus dolomieu). Estrone (E1) and atrazine (ATR) are common environmental contaminants often associated with agricultural land use. 17alpha-ethinylestrad
Authors
Jessica Kristin Leet, Catherine A. Richter, Robert Gale, Donald E. Tillitt, Jill Jenkins
By
Ecosystems Mission Area, Columbia Environmental Research Center, Wetland and Aquatic Research Center

Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection

Disease outbreaks, skin lesions, mortality events, and reproductive abnormalities have been observed in wild populations of centrarchids. The presence of estrogenic endocrine disrupting compounds (EEDCs) has been implicated as a potential causal factor for these effects. The effects of prior EEDC exposure on immune response were examined in juvenile largemouth bass (Micropterus salmoides) exposed
Authors
Jessica Kristin Leet, Justin Greer, Catherine A. Richter, Luke R. Iwanowicz, Edward Spinard, Jacquelyn McDonald, Carla M. Conway, Robert W. Gale, Donald E. Tillitt, John Hansen
By
Ecosystems Mission Area, Columbia Environmental Research Center, Eastern Ecological Science Center, Western Fisheries Research Center, Fish Health Program (FHP)

Factors affecting sampling strategies for design of an effects‐directed analysis for endocrine‐active chemicals

Effects‐directed analysis (EDA) is an important tool for identifying unknown bioactive components in a complex mixture. Such an analysis of endocrine‐active chemicals (EACs) from water sources has promising regulatory implications but also unique logistical challenges. We propose a conceptual EDA (framework) based on a critical review of EDA literature and concentrations of common EACs in waste an
Authors
Jennifer Brennan, Robert W. Gale, David Alvarez, Jason P. Berninger, Jessica Kristin Leet, Yan Li, Tyler Wagner, Donald E. Tillitt
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Ecosystems Mission Area, Columbia Environmental Research Center

Acute toxicity and clotting times of anticoagulant rodenticides to red-toothed (Odonus niger) and black (Melichthys niger) triggerfish, fathead minnow (Pimephales promelas), and largemouth bass (Micropterus salmoides)

Anticoagulant rodenticides (ARs) areused in rateradication efforts on island wildlife refuges. ARbait pellets can get into coralreefareasduring broadcasting and leadto exposure ofnon-target organisms, such as marine fishes. The objective of this study was to determine the sensitivity of representative saltwater fishes, Red-toothed triggerfish (Odonus niger) and Black triggerfish (Melichthys niger), and
Authors
Rachelle Riegerix, Mike Tanner, Robert W. Gale, Donald E. Tillitt
By
Ecosystems Mission Area, Columbia Environmental Research Center

Sensitivity of lake sturgeon (Acipenser fulvescens) early life stages to 2,3,7,8-tetrachlorodibenzo-P-dioxin and 3,3′,4,4′,5-pentachlorobiphenyl

The aquatic food web of the Great Lakes has been contaminated with polychlorinated biphenyls (PCBs) since the mid-20th century. Threats of PCB exposures to long-lived species of fish, such as lake sturgeon (Acipenser fulvescens), have been uncertain because of a lack of information on the relative sensitivity of the species. The objective of the present study was to evaluate the sensitivity of ear
Authors
Donald E. Tillitt, Justin A. Buckler, Diane Nicks, James Candrl, Rachel Claunch, Robert W. Gale, Holly J. Puglis, Edward E. Little, Tiffany L. Linbo, Mary Baker
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Ecosystems Mission Area, Columbia Environmental Research Center

Bioaccumulation of stentorin, the probable causative agent for discolored (“purple”) eggs and ovaries in blue catfish (Ictalurus furcatus) from Eufaula Lake, Oklahoma, USA

Observations of reddish to “purple” discolored eggs in the ovaries of adult female blue catfish (Ictalurus furcatus) from the northern arm of Eufaula Lake, a eutrophic multiuse impoundment in east-central Oklahoma, were first reported in 2006. Blue catfish eggs are normally cream to light yellow. Reports peaked in 2007–2008 and declined through 2009–2010; purple eggs have not been reported between
Authors
Robert W. Gale, Diana M. Papoulias, Christopher J. Schmitt
By
Ecosystems Mission Area, Columbia Environmental Research Center

Immunological and reproductive health assessment in herring gulls and black-crowned night herons in the Hudson–Raritan Estuary

Previous studies have shown inexplicable declines in breeding waterbirds within western New York/New Jersey Harbor between 1996 and 2002 and elevated polychlorinated dibenzo-p-dioxins and polychlorinated biphenyls (PCBs) in double-crested cormorant (Phalacrocorax auritus) eggs. The present study assessed associations between immune function, prefledgling survival, and selected organochlorine compo
Authors
Keith A. Grasman, Kathy R. Echols, Thomas M. May, Paul H. Peterman, Robert W. Gale, Carl E. Orazio
By
Ecosystems Mission Area, Columbia Environmental Research Center

Comparison of aliphatic hydrocarbons, polycyclic aromatic hydrocarbons, polychlorinated biphenyls, polybrominated diphenylethers, and organochlorine pesticides in Pacific sanddab (Citharichthys sordidus) from offshore oil platforms and natural reefs along

Recently, the relative exposure of Pacific sanddab (Citharichthys sordidus) to polycyclic aromatic hydrocarbons (PAHs) at oil-production platforms was reported, indicating negligible exposure to PAHs and no discernible differences between exposures at platforms and nearby natural areas sites. In this report, the potential for chronic PAH exposure in fish is reported, by measurement of recalcitrant
Authors
Robert W. Gale, Michael J. Tanner, Milton S. Love, Mary M. Nishimoto, Donna M. Schroeder
By
Ecosystems Mission Area, Columbia Environmental Research Center

Persistence and changes in bioavailability of dieldrin, DDE and heptachlor epoxide in earthworms over 45 years

The finding of dieldrin (88 ng/g), DDE (52 ng/g), and heptachlor epoxide (19 ng/g) in earthworms from experimental plots after a single moderate application (9 kg/ha) 45 years earlier attests to the remarkable persistence of these compounds in soil and their continued uptake by soil organisms. Half-lives (with 95 % confidence intervals) in earthworms, estimated from exponential decay equations, we
Authors
W. Nelson Beyer, Robert W. Gale
By
Ecosystems Mission Area, Eastern Ecological Science Center, Columbia Environmental Research Center

Comparison of concentrations and profiles of polycyclic aromatic hydrocarbon metabolites in bile of fishes from offshore oil platforms and natural reefs along the California coast

To determine the environmental consequences of decommissioning offshore oil platforms on local and regional fish populations, contaminant loads in reproducing adults were investigated at seven platform sites and adjacent, natural sites. Specimens of three species (Pacific sanddab, Citharichthys sordidus; kelp rockfish, Sebastes atrovirens; and kelp bass, Paralabrax clathratus) residing at platform
Authors
Robert W. Gale, Michael J. Tanner, Milton S. Love, Mary M. Nishimoto, Donna M. Schroeder
By
Ecosystems Mission Area, Columbia Environmental Research Center

Results of laboratory testing for diphacinone in seawater, fish, invertebrates, and soil following aerial application of rodenticide on Lehua Island, Kauai County, Hawaii, January 2009

In January 2009, rodenticide bait (Ramik Green pellets) containing the active ingredient diphacinone was aerially applied to Lehua Island. Reported herein are the results of laboratory analyses to determine diphacinone concentrations in samples of seawater, fillet of fish, soft tissue of limpets (opihi), whole-body crabs, and soil collected from Lehua Island, Kauai County, Hawaii, after aerial app
Authors
Carl E. Orazio, Michael J. Tanner, Chris Swenson, Jeffrey J. Herod, Peter Dunlevy, Robert W. Gale
By
Ecosystems Mission Area, Columbia Environmental Research Center

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