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Anthropogenic factors coupled with increased stochastic weather events (e.g., flood and drought) has caused stream temperatures to increase. Due largely to increased stream temperature, brook trout currently occupy only 70% of their historical distribution and regional models predict further range contraction as stream temperatures continue to increase.

Anthropogenic factors coupled with increased stochastic weather events (e.g., flood and drought) has caused stream temperatures to increase. Due largely to increased stream temperature, brook trout currently occupy only 70% of their historical distribution and regional models predict further range contraction as stream temperatures continue to increase.

The de-watered Turners Falls power canal taken from the S.O. Conte Lab intakes.  Juvenile lamprey were collected in the drained water canal via fyke-type traps seen in the lower left. 

Canal drawdown and cleaning of water intake at USGS EESC Conte Research Laboratory

Luke Iwanowicz and Clay Raines patiently watching samples filter through zymo columns  inside the Biosafety Level-3 lab at the Leetown, WV facility..

 Luke Iwanowicz putting samples into manifold inside the Biosafety Level-3 lab at the Leetown, WV facility.

Justin Krahulik, Logan Neal, Moussu Guira & Julie Dietze finish packing the last shipments for the field.

Luke Iwanowicz conducts RNA extractions in the Biosafety Level-3 lab at  Leetown, WV

Rachel Atkins retrieving and packaging samples from each facility, which must be stored on ice during shipment to the Leetown, WV lab

1x filtered samples processed via vacuum manifold RNA column filtration. Photos take in the BSL-3 lab are often fuzzy due to phones being kept in sealed plastic for safety.

Chelsea Delsack snaps a selfie in sample pickup PPE after packaging samples for shipment to Leetown, WV

Initial PPE kits sent to field crews for wastewater sampling to ensure proper safety protocols are followed for all waste sampling.

EESC Bird Banding Lab Team Photo Sep 15 2021_Tom O'Connell Credit.  This photos show the team of banders at the bird banding station during fall migration at Patuxent Research Refuge

Luke Iwanowicz dons multiple layers of PPE for safety prior to entering the BSL-3 lab

Rachel Atkins organizes boxes and PPE in preparation for sampling at several facilities in Utah using an oversized cargo van.

Transfer of RNA spin columns into collection tubes (with Chris Ottinger and Colleen Young)

Examples of the temperature card experiment. This additional QA step will allow more detailed tracking of sample temperature during shipping. 

 John Steiner preparing to bring wastewater samples to FedEx for shipping.

EESC Bird Banding Lab showing Matt Rogosky on Sep 15 2021 photo taken by Tom OConnell.

EESC Bird Banding Lab_Matt Rogosky and Lauren Walker_Sep 15 2021_Tom OConnell Credit

Unloading sample boxes from Fed Ex Truck into the receiving area at the Leetown Lab, Christine Densmore