USGS field team member runs cleaning solution through the autosampler installed at one of the wastewater facilities in Ohio as part of a QA test to see if cleaning procedures affect sample results.
Images
Eastern Ecological Science Center images.
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Cleaning Ohio Autosampler
USGS field team member runs cleaning solution through the autosampler installed at one of the wastewater facilities in Ohio as part of a QA test to see if cleaning procedures affect sample results.
Paperwork
Picture of Travis Smith at a wastewater facility filling out paperwork for samples collected that day in the lab van before shipping the same samples to the Leetown, WV lab.
Picture of Travis Smith at a wastewater facility filling out paperwork for samples collected that day in the lab van before shipping the same samples to the Leetown, WV lab.
Retrieving a Sample
A USGS field team member retrieving the sample bottle from a time-based sampler onsite at a wastewater treatment facility.
A USGS field team member retrieving the sample bottle from a time-based sampler onsite at a wastewater treatment facility.
Wisconsin Sample Collection
Field team member Laura Hubbard shows off a sample in the field after pickup from the wastewater facility.
Field team member Laura Hubbard shows off a sample in the field after pickup from the wastewater facility.
Composite Wastewater Sample
Image of a flow-based composite sample being collected at one of the wastewater facilities to represent a 24-hour sample.
Image of a flow-based composite sample being collected at one of the wastewater facilities to represent a 24-hour sample.
ddPCR Lab
Caitlin Beaver (WARC) setting up plates to run ddPCR in the Biosafety-level 3 lab in Leetown, WV.
Caitlin Beaver (WARC) setting up plates to run ddPCR in the Biosafety-level 3 lab in Leetown, WV.
Early Morning Sample Collection
Red biohazard bag, sealed bags with samples in bottles and a sample box on the ground.
Red biohazard bag, sealed bags with samples in bottles and a sample box on the ground.
RPS
Julie Dietze vs. Jonathon Cohl in a Rock/Paper/Scissors showdown that you'd have to see to believe!
Julie Dietze vs. Jonathon Cohl in a Rock/Paper/Scissors showdown that you'd have to see to believe!
brook trout
Anthropogenic factors coupled with increased stochastic weather events (e.g., flood and drought) has caused stream temperatures to increase. Due largely to increased stream temperature, brook trout currently occupy only 70% of their historical distribution and regional models predict further range contraction as stream temperatures continue to increase.
Anthropogenic factors coupled with increased stochastic weather events (e.g., flood and drought) has caused stream temperatures to increase. Due largely to increased stream temperature, brook trout currently occupy only 70% of their historical distribution and regional models predict further range contraction as stream temperatures continue to increase.
Temperature recording tag being surgically implanted into brook trout
Temperature recording tag being surgically implanted into brook troutAnthropogenic factors coupled with increased stochastic weather events (e.g., flood and drought) has caused stream temperatures to increase. Due largely to increased stream temperature, brook trout currently occupy only 70% of their historical distribution and regional models predict further range contraction as stream temperatures continue to increase.
Temperature recording tag being surgically implanted into brook trout
Temperature recording tag being surgically implanted into brook troutAnthropogenic factors coupled with increased stochastic weather events (e.g., flood and drought) has caused stream temperatures to increase. Due largely to increased stream temperature, brook trout currently occupy only 70% of their historical distribution and regional models predict further range contraction as stream temperatures continue to increase.
USGS EESC water intake at Conte Research Laboratory
USGS EESC water intake at Conte Research LaboratoryCanal drawdown and cleaning of water intake at USGS EESC Conte Research Laboratory
USGS EESC water intake at Conte Research Laboratory
USGS EESC water intake at Conte Research LaboratoryCanal drawdown and cleaning of water intake at USGS EESC Conte Research Laboratory
Juvenile lamprey collection in the power canal
The de-watered Turners Falls power canal taken from the S.O. Conte Lab intakes. Juvenile lamprey were collected in the drained water canal via fyke-type traps seen in the lower left.
The de-watered Turners Falls power canal taken from the S.O. Conte Lab intakes. Juvenile lamprey were collected in the drained water canal via fyke-type traps seen in the lower left.
Samples in the Manifold
Luke Iwanowicz putting samples into manifold inside the Biosafety Level-3 lab at the Leetown, WV facility.
Luke Iwanowicz putting samples into manifold inside the Biosafety Level-3 lab at the Leetown, WV facility.
Zymo Columns are Slow
Luke Iwanowicz and Clay Raines patiently watching samples filter through zymo columns inside the Biosafety Level-3 lab at the Leetown, WV facility..
Luke Iwanowicz and Clay Raines patiently watching samples filter through zymo columns inside the Biosafety Level-3 lab at the Leetown, WV facility..
Logistics Team Members
Justin Krahulik, Logan Neal, Moussu Guira & Julie Dietze finish packing the last shipments for the field.
Justin Krahulik, Logan Neal, Moussu Guira & Julie Dietze finish packing the last shipments for the field.
RNA Extraction 1
Luke Iwanowicz conducts RNA extractions in the Biosafety Level-3 lab at Leetown, WV
Luke Iwanowicz conducts RNA extractions in the Biosafety Level-3 lab at Leetown, WV
Sample Pickup
Rachel Atkins retrieving and packaging samples from each facility, which must be stored on ice during shipment to the Leetown, WV lab
Rachel Atkins retrieving and packaging samples from each facility, which must be stored on ice during shipment to the Leetown, WV lab
Processing Filtered Samples
1x filtered samples processed via vacuum manifold RNA column filtration. Photos take in the BSL-3 lab are often fuzzy due to phones being kept in sealed plastic for safety.
1x filtered samples processed via vacuum manifold RNA column filtration. Photos take in the BSL-3 lab are often fuzzy due to phones being kept in sealed plastic for safety.
Sampling is FUN
Chelsea Delsack snaps a selfie in sample pickup PPE after packaging samples for shipment to Leetown, WV
Chelsea Delsack snaps a selfie in sample pickup PPE after packaging samples for shipment to Leetown, WV
Safety First
Initial PPE kits sent to field crews for wastewater sampling to ensure proper safety protocols are followed for all waste sampling.
Initial PPE kits sent to field crews for wastewater sampling to ensure proper safety protocols are followed for all waste sampling.
EESC Bird Banding Lab at PRR on Sep 15 2021_Kyra Harvey
EESC Bird Banding Lab at PRR on Sep 15 2021_Kyra HarveyThe Eastern Ecological Science Center Bird Banding Lab at Patuxent Research Refuge on Sep 15 2021 Kyra Harvey.
EESC Bird Banding Lab at PRR on Sep 15 2021_Kyra Harvey
EESC Bird Banding Lab at PRR on Sep 15 2021_Kyra HarveyThe Eastern Ecological Science Center Bird Banding Lab at Patuxent Research Refuge on Sep 15 2021 Kyra Harvey.