Reston Microbiology Laboratory

Publications

Filter Total Items: 5
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Year Published: 2017

Detection of diazotrophy in the acetylene-fermenting anaerobe Pelobacter sp. strain SFB93

Acetylene (C2H2) is a trace constituent of the present Earth's oxidizing atmosphere, reflecting a mixture of terrestrial and marine emissions from anthropogenic, biomass-burning, and unidentified biogenic sources. Fermentation of acetylene was serendipitously discovered during C2H2 block assays of N2O reductase, and Pelobacter acetylenicus was...

Akob, Denise M.; Baesman, Shaun; Sutton, John M.; Fierst, Janna L.; Mumford, Adam; Shrestha, Yesha; Poret-Peterson, Amisha T.; Bennett, Stacy; Dunlap, Darren S.; Haase, Karl B.; Oremland, Ronald S.
Akob, D. M.; Baesman, S. M.; Sutton, J. M.; Fierst, J. L.; Mumford, A. C.; Shrestha, Y.; Poret-Peterson, A. T.; Bennett, S.; Dunlap, D. S.; Haase, K. B.; Oremland, R. S., (2017) Detection of diazotrophy in the acetylene-fermenting anaerobe Pelobacter sp. strain SFB93. Applied and Environmental Microbiology, 83(17): e01198-17, 10.1128/aem.01198-17.

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Year Published: 2017

Microbially mediated barite dissolution in anoxic brines

Fluids injected into shale formations during hydraulic fracturing of black shale return with extraordinarily high total-dissolved-solids (TDS) and high concentrations of barium (Ba) and radium (Ra). Barite, BaSO4, has been implicated as a possible source of Ba as well as a problematic mineral scale that forms on internal well surfaces, often in...

Ouyang, Bingjie; Akob, Denise M.; Dunlap, Darren S.; Renock, Devon
Ouyang, B., D. M. Akob, D. S. Dunlap, and D. Renock. 2017. Microbially mediated barite dissolution in anoxic brines. Applied Geochemistry, 76: 51-59.

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Year Published: 2016

Environmental drivers of differences in microbial community structure in crude oil reservoirs across a methanogenic gradient

Stimulating in situ microbial communities in oil reservoirs to produce natural gas is a potentially viable strategy for recovering additional fossil fuel resources following traditional recovery operations. Little is known about what geochemical parameters drive microbial population dynamics in biodegraded, methanogenic oil reservoirs. We...

Shelton, Jenna L.; Akob, Denise M.; McIntosh, Jennifer C.; Fierer, Noah; Spear, John R.; Warwick, Peter D.; McCray, John E.
Shelton, J. L., Akob, D. M., McIntosh, J. C., Fierer, N., Spear, J. R., Warwick, P. D., & McCray, J. E. (2016). Environmental Drivers of Differences in Microbial Community Structure in Crude Oil Reservoirs across a Methanogenic Gradient. Frontiers in Microbiology, 7.

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Year Published: 2016

Endocrine disrupting activities of surface water associated with a West Virginia oil and gas industry wastewater disposal site

Currently, >95% of end disposal of hydraulic fracturing wastewater from unconventional oil and gas operations in the US occurs via injection wells. Key data gaps exist in understanding the potential impact of underground injection on surface water quality and environmental health. The goal of this study was to assess endocrine disrupting...

Kassotis, Christopher D.; Iwanowicz, Luke R. ; Akob, Denise M.; Cozzarelli, Isabelle M.; Mumford, Adam; Orem, William H.; Nagel, Susan C.
Kassotis, C.D., L.R. Iwanowicz, D.M. Akob, I.M. Cozzarelli, A.C. Mumford, W.H. Orem, and S.C. Nagel. 2016. Endocrine Disrupting Activities of Surface Water Associated with a West Virginia Oil and Gas Industry Wastewater Disposal Site. Science of the Total Environment, 557–558: 901–910.

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Year Published: 2016

Wastewater disposal from unconventional oil and gas development degrades stream quality at a West Virginia injection facility

The development of unconventional oil and gas (UOG) resources has rapidly increased in recent years; however, the environmental impacts and risks are poorly understood. A single well can generate millions of liters of wastewater, representing a mixture of formation brine and injected hydraulic fracturing fluids. One of the most common methods for...

Akob, Denise M.; Mumford, Adam; Orem, William H.; Engle, Mark A.; Klinges, Julia (Grace); Kent, Douglas B.; Cozzarelli, Isabelle M.
Akob, D.M., A. Mumford, W. H. Orem, M. Engle, J.G. Klinges, D. B. Kent and I. M. Cozzarelli. Wastewater Disposal from Unconventional Oil and Gas Development Degrades Stream Quality at a West Virginia Injection Facility. Environmental Science and Technology, 50 (11): 5517–5525