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Validation of a species-specific probe-based qPCR assay for the threatened meltwater stonefly, Lednia tumana, in environmental samples Validation of a species-specific probe-based qPCR assay for the threatened meltwater stonefly, Lednia tumana, in environmental samples

A probe-based quantitative real-time PCR assay was developed to detect meltwater stonefly (Lednia tumana) environmental (e)DNA in water samples. The limits of detection and quantification, respectively, were 12.1 and 58.4 gene copies for calibration standards and these values were similarly low in a relevant environmental sample matrix (8.6 and 174.2, respectively). The assay’s utility...
Authors
Patrick Ross Hutchins, Jonathan Giersch, Adam Sepulveda, Clint C. Muhlfeld

Triple oxygen isotope compositions of globally distributed soil carbonates record widespread evaporation of soil waters Triple oxygen isotope compositions of globally distributed soil carbonates record widespread evaporation of soil waters

The stable isotopic composition of pedogenic carbonates is central to many studies of past climate and topography, providing a basis for our understanding of Earth's terrestrial history. A core assumption of many applications of oxygen isotope values (δ18O) of pedogenic carbonate is that they reflect the δ18O value of precipitation (rain/snow). This assumption is violated if soil...
Authors
Julia Kelson, Tyler E. Huth, Benjamin H. Passey, Naomi E. Levin, Sierra V. Petersen, Paolo Ballato, Emily J. Beverly, Daniel O. Breecker, Gregory D. Hoke, Adam M. Hudson, Ji Haoyuan, Alexis Licht, Jay Quade

Development of a quantitative PCR assay for detecting northwest salamander (ambystoma gracile) in environmental DNA samples Development of a quantitative PCR assay for detecting northwest salamander (ambystoma gracile) in environmental DNA samples

We developed a primer and probe based quantitative PCR assay for use with environmental DNA to detect Northwest salamander (Ambystoma gracile), a species endemic to the temperate Pacific coastal region of North America. The assay targets a region in the mitochondrial DNA (mtDNA) D-loop gene. Tests of the assay were performed in silico (using the NCBI BLAST tool), in vitro (using DNA...
Authors
Marshal S. Hoy, Carl O. Ostberg

Human and infrastructure exposure to large wildfires in the United States Human and infrastructure exposure to large wildfires in the United States

An increasing number of wildfire disasters have occurred in recent years in the United States. Here we demonstrate that cumulative primary human exposure—the population residing within the perimeters of large wildfires—was 594,850 people from 2000 to 2019 across the contiguous United States (CONUS), 82% of which occurred in the western United States. Primary population exposure increased...
Authors
Arash Modaresi Rad, John T. Abatzoglou, Jason R. Kreitler, Mohammad Reza Alizadeh, Amir AghaKouchak, Nicholas Hudyma, NIcholas Nauslar, Mojtaba Sadegh

Earthquake scenarios for Quito, Ecuador; Cali, Colombia; and Santiago De Los Caballeros, Dominican Republic Earthquake scenarios for Quito, Ecuador; Cali, Colombia; and Santiago De Los Caballeros, Dominican Republic

Earthquake risk associated with Quito, Ecuador; Cali, Colombia; and Santiago de los Caballeros, Dominican Republic is examined by generating a set of hypothetical earthquake scenarios considering seismic sources, recent seismicity, and major historical earthquakes recorded in the vicinity. In this study, particular focus is given to the development of earthquake scenarios for use in...
Authors
Robert Edward Chase, Kishor S. Jaiswal, Alejandro Calderon, Hugo Yepes, Loren Goddard, Catalina Yepes-Estrada

Biotic and abiotic factors shaping bat activity in Maryland soybean fields Biotic and abiotic factors shaping bat activity in Maryland soybean fields

Bats are important pest control agents in agriculture. Yet, the underlying fine-scale biotic and abiotic mechanisms that drive their foraging behaviors and responses to insect outbreaks are unclear. Herbivore-induced plant volatiles (HIPVs) can attract both invertebrate and vertebrate natural enemies that use the chemical plant cues to locate insect prey. The ability of HIPVs to attract...
Authors
Lauren D. Maynard, W. Mark Ford, John D. Parker, Susan R. Whitehead

Validation and standardization of SPE and HPLC-UV methods for simultaneous determination of legacy and insensitive munitions Validation and standardization of SPE and HPLC-UV methods for simultaneous determination of legacy and insensitive munitions

There have been few attempts to consolidate legacy and insensitive munitions analyses. Furthermore, there are no standard methods for insensitive munitions (IM) in tissues, resulting in overlapping methods and supplementary analyses. The goal of the present study was to validate extraction and instrumental methods previously developed and address analytical methodology gaps (missing...
Authors
Austin Scircle, Ashley Kimble, Jared C. Smith, Bobbi Stromer, Samuel Beal, Jay Clausen, Thomas Georgian, Adam C. Mumford, Geoffrey Giarmo, Martin Peterson, Hart Hedgpeth, Rebecca Crouch, Anthony J. Bednar

2023 Coastal master plan: ICM-wetlands – Submerged aquatic vegetation (SAV) updates 2023 Coastal master plan: ICM-wetlands – Submerged aquatic vegetation (SAV) updates

Submerged aquatic vegetation (SAV) provides critical structural habitat for valuable nekton and wildlife species across coastal ecosystems and can buffer the negative effects of land loss. Landscape change and restoration efforts across coastal Louisiana can impact the occurrence, coverage, and species assemblages of SAV, and changes to these foundational species can have cascading...
Authors
Kristin DeMarco, Donald Schoolmaster, Brady Couvillion

2023 Coastal master plan: Landscape input data 2023 Coastal master plan: Landscape input data

Coastal Louisiana is a complex landscape. The composition of the landscape, as well as the processes which influence said landscape, vary in both space and time. The models used in the 2023 Coastal Master Plan must attempt to reflect that spatial and temporal variability. It is therefore of the utmost importance that the spatial data sets upon which the models are initialized are of the...
Authors
Brady Couvillion

Vortex trapping of sand grains over ripples under oscillatory flow Vortex trapping of sand grains over ripples under oscillatory flow

Sand ripples significantly impact morphodynamics in the nearshore by generating coherent vortices, which can transport suspended sediment to greater heights in the water column than above flat beds. Coherent vortices can trap sediment grains if the settling velocity of the grain is smaller than the maximum vertical fluid velocity in the vortex (Nielsen 1992). Particle image and tracking
Authors
Donya P. Frank-Gilchrist, Allison Penko, Margaret L. Palmsten, Joseph Calantoni

Automated georeferencing and feature extraction of geologic maps and mineral sites Automated georeferencing and feature extraction of geologic maps and mineral sites

The predictive power of mineral prospectivity analysis depends on high quality, spatially accurate, analysis-ready datasets. Of paramount importance are geologic maps and mineral site data, but the state of readiness for utilizing these datasets remains sub-optimal for advanced computational techniques. As the U.S. Geological Survey (USGS) fulfils its mission to map the distribution of...
Authors
Graham W. Lederer, Joshua Mark Rosera, Margaret A. Goldman, Garth E. Graham, Asitang Mishra, Amanda Towler, Brian Wilson, Dustin Graf, Michael Milano, Elizabeth Roberts, Gabrielle Hedrick, Carsten Oertel, Anastassios Dardas, Thomas McEntee

The "H," "A," and "B" of a HAB: A definitional framework The "H," "A," and "B" of a HAB: A definitional framework

The use of the phrase “harmful algal bloom” and the acronym HAB originated in the marine science world, and referred to blooms also known as red tides, which can kill fish and sea life. The organisms that make up marine HABs generally do not thrive in lakes. In freshwater, HABs are most often associated with blooms of toxin-producing cyanobacteria. The term HAB started to be used broadly...
Authors
Rebecca M. Gorney, Jennifer L. Graham, Jennifer C. Murphy
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