An antibiotic resistance gene (ARG) microarray in the Real-Time PCR (RT-PCR) machine.
James Evans, Ph.D.
James is a postdoctoral Research Marine Biologist with the St. Petersburg Coastal and Marine Science Center, where he is part of the Coral Microbial Ecology Laboratory.
James is interested in the intersection between microbial symbiosis and host function and fitness, with a particular focus on marine conservation. His current research with the USGS uses molecular approaches to investigate the causative agent(s) of stony coral tissue loss disease (SCTLD), an ongoing coral disease outbreak currently impacting reefs throughout Florida and the wider Caribbean. His prior research experience includes using marine invertebrate microbiomes to better understand the invasive potential of nonnative marine species and as a tool to help distinguish between morphologically indistinguishable cryptic lineages.
Science and Products
Stony Coral Tissue Loss Disease – Investigating Possible Pathogens and Transmission Mechanisms
MERMAID - Metagenomic Examinations of Reefs; Microbial Assessments Including Disease
Integrating Science and Management to Assist with the Response to Stony Coral Tissue Loss Disease
Testing Treatments Against Parasitic Scuticociliate (Philaster apodigitiformis) that Causes Mass Mortality Among Sea Urchins (Diadema antillarum) - Results
Prokaryotic Communities Shed by Diseased and Healthy Coral (Diploria labyrinthiformis, Pseudodiploria strigosa, Montastraea cavernosa, Colpophyllia natans, and Orbicella faveolata) into Filtered Seawater Mesocosms - Raw and Processed Data
The files in this data release are those referenced in the journal article by Evans and others (2023) entitled 'Investigating microbial size classes associated with the transmission of stony coral tissue loss disease (SCTLD)'. The files contain an amplicon sequence variant (ASV) table and the raw 16S rRNA gene amplicon files from fifty-six 0.22-micrometer (µm) pore size filters, as well as six rea
Expert assessments of hypotheses concerning the etiological agent(s) of Stony Coral Tissue Loss Disease collected during a rapid prototyping project
Prokaryotic Communities From Marine Biofilms Formed on Stainless Steel Plates in Coral Mesocosms - Raw and Processed Data
The files in this data release are those referenced in the journal article by Evans and others (2022) entitled "Ship Biofilms as Potential Reservoirs of Stony Coral Tissue Loss Disease." They contain an amplicon sequence variant (ASV) table and the raw 16S ribosomal ribonucleic acid (rRNA) gene amplicon deoxyribonucleic acid (DNA) sequence files from 15 microbial communities (sample names: CnD16B,
Bacterial Communities Shed by Montastraea cavernosa Coral Fragments into Filtered Seawater Mesocosms-Raw Data
An antibiotic resistance gene (ARG) microarray in the Real-Time PCR (RT-PCR) machine.
Biofilms are mats of microorganisms that stick together and form a layer, or “film,” on and within objects in the water—including ships—which could represent one possible mechanism for the spread of microbes (and potentially SCTLD’s unknown causative agent) from one region to another. To test this theory, scientists at the St.
Biofilms are mats of microorganisms that stick together and form a layer, or “film,” on and within objects in the water—including ships—which could represent one possible mechanism for the spread of microbes (and potentially SCTLD’s unknown causative agent) from one region to another. To test this theory, scientists at the St.
Evaluation of in vitro treatments against the causative agent of Diadema antillarum scuticociliatosis (DaSc)
Transglobal spread of an ecologically relevant sea urchin parasite
Investigating microbial size classes associated with the transmission of stony coral tissue loss disease (SCTLD)
Effective treatment and prevention of any disease necessitates knowledge of the causative agent, yet the causative agents of most coral diseases remain unknown, in part due to the difficulty of distinguishing the pathogenic microbe(s) among the complex microbial backdrop of coral hosts. Stony coral tissue loss disease (SCTLD) is a particularly destructive disease of unknown etiology, capable of tr
A scuticociliate causes mass mortality of Diadema antillarum in the Caribbean Sea
Rapid prototyping for quantifying belief weights of competing hypotheses about emergent diseases
A meta-analysis of the stony coral tissue loss disease microbiome finds key bacteria in unaffected and lesion tissue in diseased colonies
Stony coral tissue loss disease (SCTLD) has been causing significant whole colony mortality on reefs in Florida and the Caribbean. The cause of SCTLD remains unknown, with the limited concurrence of SCTLD-associated bacteria among studies. We conducted a meta-analysis of 16S ribosomal RNA gene datasets generated by 16 field and laboratory SCTLD studies to find consistent bacteria associated with S
Biofilms as potential reservoirs of stony coral tissue loss disease
Since 2014, corals throughout Florida’s Coral Reef have been plagued by an epizootic of unknown etiology, colloquially termed stony coral tissue loss disease (SCTLD). Although in Florida the movement of this waterborne coral disease has been consistent with natural transport via water currents, outbreaks in the Caribbean have been more sporadic, with infections occurring in locations inconsistent
Combining tangential flow filtration and size fractionation of mesocosm water as a method for the investigation of waterborne coral diseases
Science and Products
Stony Coral Tissue Loss Disease – Investigating Possible Pathogens and Transmission Mechanisms
MERMAID - Metagenomic Examinations of Reefs; Microbial Assessments Including Disease
Integrating Science and Management to Assist with the Response to Stony Coral Tissue Loss Disease
Testing Treatments Against Parasitic Scuticociliate (Philaster apodigitiformis) that Causes Mass Mortality Among Sea Urchins (Diadema antillarum) - Results
Prokaryotic Communities Shed by Diseased and Healthy Coral (Diploria labyrinthiformis, Pseudodiploria strigosa, Montastraea cavernosa, Colpophyllia natans, and Orbicella faveolata) into Filtered Seawater Mesocosms - Raw and Processed Data
The files in this data release are those referenced in the journal article by Evans and others (2023) entitled 'Investigating microbial size classes associated with the transmission of stony coral tissue loss disease (SCTLD)'. The files contain an amplicon sequence variant (ASV) table and the raw 16S rRNA gene amplicon files from fifty-six 0.22-micrometer (µm) pore size filters, as well as six rea
Expert assessments of hypotheses concerning the etiological agent(s) of Stony Coral Tissue Loss Disease collected during a rapid prototyping project
Prokaryotic Communities From Marine Biofilms Formed on Stainless Steel Plates in Coral Mesocosms - Raw and Processed Data
The files in this data release are those referenced in the journal article by Evans and others (2022) entitled "Ship Biofilms as Potential Reservoirs of Stony Coral Tissue Loss Disease." They contain an amplicon sequence variant (ASV) table and the raw 16S ribosomal ribonucleic acid (rRNA) gene amplicon deoxyribonucleic acid (DNA) sequence files from 15 microbial communities (sample names: CnD16B,
Bacterial Communities Shed by Montastraea cavernosa Coral Fragments into Filtered Seawater Mesocosms-Raw Data
An antibiotic resistance gene (ARG) microarray in the Real-Time PCR (RT-PCR) machine.
An antibiotic resistance gene (ARG) microarray in the Real-Time PCR (RT-PCR) machine.
Biofilms are mats of microorganisms that stick together and form a layer, or “film,” on and within objects in the water—including ships—which could represent one possible mechanism for the spread of microbes (and potentially SCTLD’s unknown causative agent) from one region to another. To test this theory, scientists at the St.
Biofilms are mats of microorganisms that stick together and form a layer, or “film,” on and within objects in the water—including ships—which could represent one possible mechanism for the spread of microbes (and potentially SCTLD’s unknown causative agent) from one region to another. To test this theory, scientists at the St.
Evaluation of in vitro treatments against the causative agent of Diadema antillarum scuticociliatosis (DaSc)
Transglobal spread of an ecologically relevant sea urchin parasite
Investigating microbial size classes associated with the transmission of stony coral tissue loss disease (SCTLD)
Effective treatment and prevention of any disease necessitates knowledge of the causative agent, yet the causative agents of most coral diseases remain unknown, in part due to the difficulty of distinguishing the pathogenic microbe(s) among the complex microbial backdrop of coral hosts. Stony coral tissue loss disease (SCTLD) is a particularly destructive disease of unknown etiology, capable of tr
A scuticociliate causes mass mortality of Diadema antillarum in the Caribbean Sea
Rapid prototyping for quantifying belief weights of competing hypotheses about emergent diseases
A meta-analysis of the stony coral tissue loss disease microbiome finds key bacteria in unaffected and lesion tissue in diseased colonies
Stony coral tissue loss disease (SCTLD) has been causing significant whole colony mortality on reefs in Florida and the Caribbean. The cause of SCTLD remains unknown, with the limited concurrence of SCTLD-associated bacteria among studies. We conducted a meta-analysis of 16S ribosomal RNA gene datasets generated by 16 field and laboratory SCTLD studies to find consistent bacteria associated with S
Biofilms as potential reservoirs of stony coral tissue loss disease
Since 2014, corals throughout Florida’s Coral Reef have been plagued by an epizootic of unknown etiology, colloquially termed stony coral tissue loss disease (SCTLD). Although in Florida the movement of this waterborne coral disease has been consistent with natural transport via water currents, outbreaks in the Caribbean have been more sporadic, with infections occurring in locations inconsistent