Luke R Iwanowicz, Ph.D. (Former Employee)
Science and Products
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In vitro immune functions in thiamine-replete and -depleted lake trout (Salvelinus namaycush)
In this study we examined the impacts of in vivo thiamine deficiency on lake trout leukocyte function measured in vitro. When compared outside the context of individual-specific thiamine concentrations no significant differences were observed in leukocyte bactericidal activity or in concanavalin A (Con A), and phytohemagglutinin-P (PHA-P) stimulated leukocyte proliferation. Placing immune function
Authors
Christopher A. Ottinger, Dale C. Honeyfield, Christine L. Densmore, Luke R. Iwanowicz
Monitoring of wild fish health at selected sites in the Great Lakes Basin: methods and preliminary results
During fall 2010 and spring 2011, a total of 119 brown bullhead (Ameiurus nebulosus), 136 white sucker (Catostomus commersoni), 73 smallmouth bass (Micropterus dolomieu), and 59 largemouth bass (M. salmoides) were collected from seven Great Lakes Basin Areas of Concern and one Reference Site. Comprehensive fish health assessments were conducted in order to document potential adverse affects from e
Authors
Vicki Blazer, Patricia M. Mazik, Luke R. Iwanowicz, Ryan Braham, Cassidy Hahn, Heather L. Walsh, Adam Sperry
Transgenic zebrafish reveal tissue-specific differences in estrogen signaling in response to environmental water samples
Background: Environmental endocrine disruptors (EED) are exogenous chemicals that mimic endogenous hormones, such as estrogens. Previous studies using a zebrafish transgenic reporter demonstrated that the EEDs bisphenol A and genistein preferentially activate estrogen receptors (ER) in the larval heart compared to the liver. However, it was not known whether the transgenic zebrafish reporter was s
Authors
Daniel A. Gorelick, Luke R. Iwanowicz, Alice L. Hung, Vicki Blazer, Marnie E. Halpern
Biological effects-based tools for monitoring impacted surface waters in the Great Lakes: a multiagency program in support of the Great Lakes Restoration Initiative
There is increasing demand for the implementation of effects-based monitoring and surveillance (EBMS) approaches in the Great Lakes Basin to complement traditional chemical monitoring. Herein, we describe an ongoing multiagency effort to develop and implement EBMS tools, particularly with regard to monitoring potentially toxic chemicals and assessing Areas of Concern (AOCs), as envisioned by the G
Authors
Drew R. Ekman, Gerald T. Ankley, Vicki Blazer, Timothy W. Collette, Natàlia Garcia-Reyero, Luke R. Iwanowicz, Zachary G. Jorgensen, Kathy Lee, Pat M. Mazik, David H. Miller, Edward J. Perkins, Edwin T. Smith, Joseph E. Tietge, Daniel L. Villeneuve
Identification of largemouth bass virus in the introduced Northern snakehead inhabiting the Cheasapeake Bay watershed
The Northern Snakehead Channa argus is an introduced species that now inhabits the Chesapeake Bay. During a preliminary survey for introduced pathogens possibly harbored by these fish in Virginia waters, a filterable agent was isolated from five specimens that produced cytopathic effects in BF-2 cells. Based on PCR amplification and partial sequencing of the major capsid protein (MCP), DNA polymer
Authors
Luke R. Iwanowicz, Christine L. Densmore, Cassidy M. Hahn, Phillip McAllister, John Odenkirk
Differential expression profiles of microRNA in the little brown bat (Myotis lucifugus) associated with white nose syndrome affected and unaffected individuals
First documented in New York State in 2006, white nose syndrome (WNS) quickly became the leading cause of mortality in hibernating bat species in the United States. WNS is caused by a psychrophilic fungus, Geomyces destructans. Clinical signs of this pathogen are expressed as a dusty white fungus predominately around the nose and on the wings of affected bats. Relatively new biomarkers, such as mi
Authors
D.D. Iwanowicz, L. R. Iwanowicz, N.P. Hitt, T.L. King
Chemical contaminants in water and sediment near fish nesting sites in the Potomac River basin: determining potential exposures to smallmouth bass (Micropterus dolomieu)
The Potomac River basin is an area where a high prevalence of abnormalities such as testicular oocytes (TO), skin lesions, and mortality has been observed in smallmouth bass (SMB, Micropterus dolomieu). Previous research documented a variety of chemicals in regional streams, implicating chemical exposure as one plausible explanation for these biological effects. Six stream sites in the Potomac bas
Authors
Dana W. Kolpin, Vicki Blazer, James L. Gray, Michael J. Focazio, John A. Young, David A. Alvarez, Luke R. Iwanowicz, William T. Foreman, Edward T. Furlong, Gary K. Speiran, Steven D. Zaugg, Laura E. Hubbard, Michael T. Meyer, Mark W. Sandstrom, Larry B. Barber
Reproductive health of yellow perch Perca flavescens in selected tributaries of the Chesapeake Bay
Reduced recruitment of yellow perch has been noted for a number of years in certain urbanized watersheds (South and Severn Rivers) of the Chesapeake Bay. Other rapidly developing watersheds such as Mattawoman Creek are more recently showing evidence of reduced recruitment of anadromous fishes. In this study, we used a battery of biomarkers to better document the reproductive health of adult yellow
Authors
Vicki Blazer, Alfred E. Pinkney, Jill A. Jenkins, Luke R. Iwanowicz, Steven Minkkinen, Rassa O. Draugelis-Dale, James H. Uphoff
Bacterial etiology in diseased fishes, primarily smallmouth bass Micropterus dolomieu, within the Chesapeake Bay drainage in 2009-2011
No abstract available.
Authors
Clifford E. Starliper, Vicki Blazer, Luke R. Iwanowicz, Heather L. Walsh
Prevalent flucocorticoid and androgen activity in US water sources
Contamination of the environment with endocrine disrupting chemicals (EDCs) is a major health concern. The presence of estrogenic compounds in water and their deleterious effect are well documented. However, detection and monitoring of other classes of EDCs is limited. Here we utilize a high-throughput live cell assay based on sub-cellular relocalization of GFP-tagged glucocorticoid and androgen r
Authors
Diana A. Stavreva, Anuja A. George, Paul Klausmeyer, Lyuba Varticovski, Daniel Sack, Ty C. Voss, R. Louis Schiltz, Vicki Blazer, Luke R. Iwanowicz, Gordon L. Hager
Histologic, immunologic and endocrine biomarkers indicate contaminant effects in fishes of the Ashtabula River
The use of fish as sentinels of aquatic ecosystem health is a biologically relevant approach to environmental monitoring and assessment. We examined the health of the Ashtabula River using histologic, immunologic, and endocrine biomarkers in brown bullhead (BB; Ameiurus nebulosus) and largemouth bass (Micropterus salmoides) and compared fish collected from a reference site (Conneaut Creek). Season
Authors
L. R. Iwanowicz, V. S. Blazer, N.P. Hitt, S. D. McCormick, D.S. Devault, C. A. Ottinger
Impact of thiamine deficiency on T-cell dependent and T-cell independent antibody production in lake trout
Lake trout Salvelinus namaycush on thiamine-replete and thiamine-depleted diets were evaluated for the effects of thiamine status on in vivo responses to the T-dependent antigen trinitophenol (TNP)-keyhole limpet hemocyanin (TNP-KLH), the T-independent antigen trinitrophenol-lipolysaccaharide (TNP-LPS), or Dulbecco's phosphate-buffered saline (DPBS; negative control fish). Plasma antibody concentr
Authors
Christopher A. Ottinger, Dale C. Honeyfield, Christine L. Densmore, Luke R. Iwanowicz
Non-USGS Publications**
Iwanowicz, L., L Brown, B Eltz, F Juanes and J Murt (2004) Book Review. In a Perfect Ocean: The state of fisheries and ecosystems in the North Atlantic Ocean. Reviews in Fish Biology and Fisheries.14: 149-151.
McIntire, M., L R Iwanowicz and A E Goodwin (2003) Molecular, physical and clinical evidence that Golden Shiner Virus (GSV) and Grass Carp Reovirus (GCRV) are variants of the same virus. Journal of Aquatic Animal Health. 15: 257-263.
Iwanowicz, L.R. and A E Goodwin (2002) Characterization of a new bacilliform fathead minnow rhabdovirus that produces syncytia in tissue culture. Archives of Virology 147: 889 – 916.
Iwanowicz, L.R. A E Goodwin and J Harshbarger (2001) Embryonal rhabdomyosarcoma of the giant gourami, Colisa fasciata (Bloch & Schneider). Journal of Fish Diseases 24:177-179
Iwanowicz, L.R. A E Goodwin and N Heil (2000) A small RNA virus isolated from apparently healthy wild sandbar shiners, Notropis scepticus. Journal of Fish Diseases 23: 349-352.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Science and Products
Filter Total Items: 13
Filter Total Items: 22
No Result Found
Filter Total Items: 103
In vitro immune functions in thiamine-replete and -depleted lake trout (Salvelinus namaycush)
In this study we examined the impacts of in vivo thiamine deficiency on lake trout leukocyte function measured in vitro. When compared outside the context of individual-specific thiamine concentrations no significant differences were observed in leukocyte bactericidal activity or in concanavalin A (Con A), and phytohemagglutinin-P (PHA-P) stimulated leukocyte proliferation. Placing immune function
Authors
Christopher A. Ottinger, Dale C. Honeyfield, Christine L. Densmore, Luke R. Iwanowicz
Monitoring of wild fish health at selected sites in the Great Lakes Basin: methods and preliminary results
During fall 2010 and spring 2011, a total of 119 brown bullhead (Ameiurus nebulosus), 136 white sucker (Catostomus commersoni), 73 smallmouth bass (Micropterus dolomieu), and 59 largemouth bass (M. salmoides) were collected from seven Great Lakes Basin Areas of Concern and one Reference Site. Comprehensive fish health assessments were conducted in order to document potential adverse affects from e
Authors
Vicki Blazer, Patricia M. Mazik, Luke R. Iwanowicz, Ryan Braham, Cassidy Hahn, Heather L. Walsh, Adam Sperry
Transgenic zebrafish reveal tissue-specific differences in estrogen signaling in response to environmental water samples
Background: Environmental endocrine disruptors (EED) are exogenous chemicals that mimic endogenous hormones, such as estrogens. Previous studies using a zebrafish transgenic reporter demonstrated that the EEDs bisphenol A and genistein preferentially activate estrogen receptors (ER) in the larval heart compared to the liver. However, it was not known whether the transgenic zebrafish reporter was s
Authors
Daniel A. Gorelick, Luke R. Iwanowicz, Alice L. Hung, Vicki Blazer, Marnie E. Halpern
Biological effects-based tools for monitoring impacted surface waters in the Great Lakes: a multiagency program in support of the Great Lakes Restoration Initiative
There is increasing demand for the implementation of effects-based monitoring and surveillance (EBMS) approaches in the Great Lakes Basin to complement traditional chemical monitoring. Herein, we describe an ongoing multiagency effort to develop and implement EBMS tools, particularly with regard to monitoring potentially toxic chemicals and assessing Areas of Concern (AOCs), as envisioned by the G
Authors
Drew R. Ekman, Gerald T. Ankley, Vicki Blazer, Timothy W. Collette, Natàlia Garcia-Reyero, Luke R. Iwanowicz, Zachary G. Jorgensen, Kathy Lee, Pat M. Mazik, David H. Miller, Edward J. Perkins, Edwin T. Smith, Joseph E. Tietge, Daniel L. Villeneuve
Identification of largemouth bass virus in the introduced Northern snakehead inhabiting the Cheasapeake Bay watershed
The Northern Snakehead Channa argus is an introduced species that now inhabits the Chesapeake Bay. During a preliminary survey for introduced pathogens possibly harbored by these fish in Virginia waters, a filterable agent was isolated from five specimens that produced cytopathic effects in BF-2 cells. Based on PCR amplification and partial sequencing of the major capsid protein (MCP), DNA polymer
Authors
Luke R. Iwanowicz, Christine L. Densmore, Cassidy M. Hahn, Phillip McAllister, John Odenkirk
Differential expression profiles of microRNA in the little brown bat (Myotis lucifugus) associated with white nose syndrome affected and unaffected individuals
First documented in New York State in 2006, white nose syndrome (WNS) quickly became the leading cause of mortality in hibernating bat species in the United States. WNS is caused by a psychrophilic fungus, Geomyces destructans. Clinical signs of this pathogen are expressed as a dusty white fungus predominately around the nose and on the wings of affected bats. Relatively new biomarkers, such as mi
Authors
D.D. Iwanowicz, L. R. Iwanowicz, N.P. Hitt, T.L. King
Chemical contaminants in water and sediment near fish nesting sites in the Potomac River basin: determining potential exposures to smallmouth bass (Micropterus dolomieu)
The Potomac River basin is an area where a high prevalence of abnormalities such as testicular oocytes (TO), skin lesions, and mortality has been observed in smallmouth bass (SMB, Micropterus dolomieu). Previous research documented a variety of chemicals in regional streams, implicating chemical exposure as one plausible explanation for these biological effects. Six stream sites in the Potomac bas
Authors
Dana W. Kolpin, Vicki Blazer, James L. Gray, Michael J. Focazio, John A. Young, David A. Alvarez, Luke R. Iwanowicz, William T. Foreman, Edward T. Furlong, Gary K. Speiran, Steven D. Zaugg, Laura E. Hubbard, Michael T. Meyer, Mark W. Sandstrom, Larry B. Barber
Reproductive health of yellow perch Perca flavescens in selected tributaries of the Chesapeake Bay
Reduced recruitment of yellow perch has been noted for a number of years in certain urbanized watersheds (South and Severn Rivers) of the Chesapeake Bay. Other rapidly developing watersheds such as Mattawoman Creek are more recently showing evidence of reduced recruitment of anadromous fishes. In this study, we used a battery of biomarkers to better document the reproductive health of adult yellow
Authors
Vicki Blazer, Alfred E. Pinkney, Jill A. Jenkins, Luke R. Iwanowicz, Steven Minkkinen, Rassa O. Draugelis-Dale, James H. Uphoff
Bacterial etiology in diseased fishes, primarily smallmouth bass Micropterus dolomieu, within the Chesapeake Bay drainage in 2009-2011
No abstract available.
Authors
Clifford E. Starliper, Vicki Blazer, Luke R. Iwanowicz, Heather L. Walsh
Prevalent flucocorticoid and androgen activity in US water sources
Contamination of the environment with endocrine disrupting chemicals (EDCs) is a major health concern. The presence of estrogenic compounds in water and their deleterious effect are well documented. However, detection and monitoring of other classes of EDCs is limited. Here we utilize a high-throughput live cell assay based on sub-cellular relocalization of GFP-tagged glucocorticoid and androgen r
Authors
Diana A. Stavreva, Anuja A. George, Paul Klausmeyer, Lyuba Varticovski, Daniel Sack, Ty C. Voss, R. Louis Schiltz, Vicki Blazer, Luke R. Iwanowicz, Gordon L. Hager
Histologic, immunologic and endocrine biomarkers indicate contaminant effects in fishes of the Ashtabula River
The use of fish as sentinels of aquatic ecosystem health is a biologically relevant approach to environmental monitoring and assessment. We examined the health of the Ashtabula River using histologic, immunologic, and endocrine biomarkers in brown bullhead (BB; Ameiurus nebulosus) and largemouth bass (Micropterus salmoides) and compared fish collected from a reference site (Conneaut Creek). Season
Authors
L. R. Iwanowicz, V. S. Blazer, N.P. Hitt, S. D. McCormick, D.S. Devault, C. A. Ottinger
Impact of thiamine deficiency on T-cell dependent and T-cell independent antibody production in lake trout
Lake trout Salvelinus namaycush on thiamine-replete and thiamine-depleted diets were evaluated for the effects of thiamine status on in vivo responses to the T-dependent antigen trinitophenol (TNP)-keyhole limpet hemocyanin (TNP-KLH), the T-independent antigen trinitrophenol-lipolysaccaharide (TNP-LPS), or Dulbecco's phosphate-buffered saline (DPBS; negative control fish). Plasma antibody concentr
Authors
Christopher A. Ottinger, Dale C. Honeyfield, Christine L. Densmore, Luke R. Iwanowicz
Non-USGS Publications**
Iwanowicz, L., L Brown, B Eltz, F Juanes and J Murt (2004) Book Review. In a Perfect Ocean: The state of fisheries and ecosystems in the North Atlantic Ocean. Reviews in Fish Biology and Fisheries.14: 149-151.
McIntire, M., L R Iwanowicz and A E Goodwin (2003) Molecular, physical and clinical evidence that Golden Shiner Virus (GSV) and Grass Carp Reovirus (GCRV) are variants of the same virus. Journal of Aquatic Animal Health. 15: 257-263.
Iwanowicz, L.R. and A E Goodwin (2002) Characterization of a new bacilliform fathead minnow rhabdovirus that produces syncytia in tissue culture. Archives of Virology 147: 889 – 916.
Iwanowicz, L.R. A E Goodwin and J Harshbarger (2001) Embryonal rhabdomyosarcoma of the giant gourami, Colisa fasciata (Bloch & Schneider). Journal of Fish Diseases 24:177-179
Iwanowicz, L.R. A E Goodwin and N Heil (2000) A small RNA virus isolated from apparently healthy wild sandbar shiners, Notropis scepticus. Journal of Fish Diseases 23: 349-352.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government