Heather Walsh
Biography
Education:
- B.S., Environmental Studies with a Concentration in Aquatic Science, Shepherd University, Shepherdstown, WV, 2011
- M.S., Wildlife and Fisheries Management, West Virginia University, Morgantown, WV, 2014-2018
- Ph.D., Forest Resource Science Program, Wildlife and Fisheries Management Major, West Virginia University
Dr. Heather Walsh is a Post Doc Researcher that has been involved in research at the Leetown Science Center Fish Health Research Lab since 2006. She has focused her research on developing molecular techniques to better understand mechanisms associated with disease in wild fish. To serve this purpose, she has developed her skills in histopathology, in situ hybridization techniques, and laser capture microdissection. The use of these techniques has helped to understand disease observed during fish health assessments conducted in the Great Lakes and Chesapeake Bay (including the Susquehanna and Potomac River drainage). Much of the research she has been involved in has been with fish species used as indicators of environmental contamination, including smallmouth bass, brown bullhead catfish, white suckers, and yellow perch. She also uses PCR, Sanger sequencing, Nanostring technology, and RNA-Seq to help identify parasites or pathogens, for transcript abundance analyses, and de novo transcriptome development.
Science and Products
Assessment of microscopic pathology in fishes collected at sites impacted by wood tar in Pennsylvania
In an effort to determine whether fish populations in an area affected by wood tar waste exhibited health effects, fish were collected and analyzed with histopathology. Multiple species, including Mottled Sculpin (Cottus bairdii), Creek Chub (Semotilus atromaculatus), White Sucker (Catostumus commersonii), Redside Dace (Clinostomus elongatus),...
Walsh, Heather L.; Blazer, Vicki S.; Mazik, Patricia M.; Sperry, Adam J.; Pavlick, Diana
Assessment of skin and liver neoplasms in white sucker (Catostomus commersonii) collected in the Sheboygan River Area of Concern, Wisconsin, in 2017
Two hundred adult white sucker (Catostomus commersonii), age 3 years and older, were collected from the lower Sheboygan River Area of Concern in 2017, during the spring spawning run. Fish were euthanized, weighed, and measured, and any visible abnormalities were documented. Pieces of raised skin lesions as well as five to eight pieces of liver...
Blazer, Vicki S.; Walsh, Heather L.; Braham, Ryan P.; Mazik, Patricia M.
Indicators of exposure to estrogenic compounds at Great Lakes Areas of Concern: Species and site comparisons
Adverse effects resulting from potential exposure of wild fishes to estrogenic endocrine disruptors was assessed at seven United States Great Lakes Areas of Concern using biomarkers ranging from organismal (gonadosomatic indices) to tissue/plasma (histology, plasma vitellogenin) and molecular (hepatic gene transcripts) levels. Biomonitoring was...
Blazer, Vicki S.; Walsh, Heather L.; Shaw, Cassidy H.; Iwanowicz, Luke R.; Braham, Ryan P.; Mazik, Patricia Temporal evaluation of estrogenic endocrine disruption markers in smallmouth bass (Micropterus dolomieu) reveals seasonal variability in intersex
A reconnaissance project completed in 2009 identified intersex and elevated plasma vitellogenin in male smallmouth bass inhabiting the Missisquoi River, VT. In an attempt to identify the presence and seasonality of putative endocrine disrupting chemicals or other factors associated with these observations, a comprehensive reevaluation was...
Iwanowicz, Luke R.; Pinkney, A.E.; Guy, C.P.; Major, A.M.; Munney, K.; Blazer, Vicki S.; Alvarez, David; Walsh, Heather L.; Sperry, Adam J.; Sanders, Lakyn R.; Smith, D.R. Pharmaceuticals, hormones, pesticides, and other bioactive contaminants in water, sediment, and tissue from Rocky Mountain National Park, 2012–2013
Pharmaceuticals, hormones, pesticides, and other bioactive contaminants (BCs) are commonly detected in surface water and bed sediment in urban and suburban areas, but these contaminants are understudied in remote locations. In Rocky Mountain National Park (RMNP), Colorado, USA, BCs may threaten the reproductive success and survival of native...
Battaglin, William A.; Bradley, Paul M.; Iwanowicz, Luke R.; Journey, Celeste A.; Walsh, Heather L.; Blazer, Vicki S.
Assessment of skin and liver neoplasms in brown bullhead (Ameiurus nebulosus) collected at the Ashtabula River Area of Concern and associated reference site, Ohio, in 2016
Brown bullhead (Ameiurus nebulosus) is a commonly used indicator species for tumor surveys at Great Lakes Areas of Concern. The “fish tumors or other deformities” is one of the beneficial use impairments at the Ashtabula River Area of Concern. In May 2016, 150 brown bullhead were collected in the lower Ashtabula River and 150 were collected in the...
Blazer, Vicki S.; Walsh, Heather L.; Braham, Ryan P. Contaminant-associated health effects in fishes from the Ottawa and Ashtabula Rivers, Ohio
The health of resident fishes serves as a biologically relevant barometer of aquatic ecosystem integrity. Here, the health of the Ottawa River and Ashtabula River (both within the Lake Erie Basin) were assessed using morphological and immunological biomarkers in brown bullheads (Ameiurus nebulosus) and largemouth bass (Micropterus salmoides)....
Iwanowicz, Luke R.; Blazer, Vicki S.; Walsh, Heather L.; Shaw, Cassidy H.; DeVault, David S.; Banda, Jo A. Tumours in white suckers from Lake Michigan tributaries: Pathology and prevalence
The prevalence and histopathology of neoplastic lesions were assessed in white suckerCatostomus commersonii captured at two Lake Michigan Areas of Concern (AOCs), the Sheboygan River and Milwaukee Estuary. Findings were compared to those observed at two non-AOC sites, the Root and Kewaunee rivers. At each site, approximately 200 adult suckers were...
Blazer, Vicki S.; Walsh, H.L.; Braham, R.P.; Hahn, C. M.; Mazik, P.; McIntyre, P.B. Evidence of estrogenic endocrine disruption in smallmouth and largemouth bass inhabiting Northeast U.S. National Wildlife Refuge waters: A reconnaissance study
Intersex as the manifestation of testicular oocytes (TO) in male gonochoristic fishes has been used as an indicator of estrogenic exposure. Here we evaluated largemouth bass (Micropterus salmoides) or smallmouth bass (Micropterus dolomieu) form 19 National Wildlife Refuges (NWRs) in the Northeast U.S. inhabiting waters on or near NWR lands for...
Iwanowicz, Luke R. ; Blazer, Vicki S.; Pinkney, A.E.; Guy, C.P.; Major, A.M.; Munney, K.; Mierzykowski, S.; Lingenfelser, S.; Secord, A.; Patnode, K.; Kubiak, T.J.; Stern, C.; Hahn, Cassidy M.; Iwanowicz, Deborah D.; Walsh, Heather L.; Sperry, Adam J. Reproductive health indicators of fishes from Pennsylvania watersheds: association with chemicals of emerging concern
Fishes were collected at 16 sites within the three major river drainages (Delaware, Susquehanna, and Ohio) of Pennsylvania. Three species were evaluated for biomarkers of estrogenic/antiandrogenic exposure, including plasma vitellogenin and testicular oocytes in male fishes. Smallmouth bass Micropterus dolomieu, white sucker Catostomus commersonii...
Blazer, V.S.; Iwanowicz, D.D.; Walsh, H.L.; Sperry, A.J.; Iwanowicz, L.R.; Alvarez, D.A.; Brightbill, R.A.; Smith, G.; Foreman, W.T.; Manning, R.
Assessment of the fish tumor beneficial use impairment in brown bullhead (Ameiurus nebulosus) at selected Great Lakes Areas of Concern
A total of 878 adult Brown Bullhead were collected at 11 sites within the Lake Erie and Lake Ontario drainages from 2011 to 2013. The sites included seven Areas of Concern (AOC; 670 individuals), one delisted AOC (50 individuals) and three non-AOC sites (158 individuals) used as reference sites. These fish were used to assess the “fish tumor or...
Blazer, Vicki; Mazik, Patricia M.; Iwanowicz, Luke R.; Braham, Ryan P.; Hahn, Cassidy M.; Walsh, Heather L.; Sperry, Adam J.
Monitoring of wild fish health at selected sites in the Great Lakes Basin: methods and preliminary results
During fall 2010 and spring 2011, a total of 119 brown bullhead (Ameiurus nebulosus), 136 white sucker (Catostomus commersoni), 73 smallmouth bass (Micropterus dolomieu), and 59 largemouth bass (M. salmoides) were collected from seven Great Lakes Basin Areas of Concern and one Reference Site. Comprehensive fish health assessments were conducted in...
Blazer, Vicki; Mazik, Patricia M.; Iwanowicz, Luke R.; Braham, Ryan; Hahn, Cassidy; Walsh, Heather L.; Sperry, Adam