Western Fisheries Research Center

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ELISA for Renibacterium salmoninarum Thumb
February 26, 2020

WFRC BACT2 ELISA for Renibacterium salmoninarum (pdf)

Title: Double polyclonal enzyme-linked immunosorbent assay (ELISA) for the detection of Renibacterium salmonarium

Number: BACT-2

Version: 02

Purpose: To detect Renibacterium salmoninarum soluble antigen in fish tissues or fluids

DFAT for Renibacterium salmonarum Thumb
February 26, 2020

WFRC BACT3 DFAT for Renibacterium salmoninarum (pdf)

Title: Direct Fluorescent Antibody Test (DFAT) for the detection of Renibacterium salmoninarum in tissues

Number: BACT-3

Version: 02 Created December 14, 2011

Purpose: Detection of Renibacterium salmoninarum cells in fish tissues or culture

MF-FAT for Renibacterium salmoninarum Thumb
February 26, 2020

WFRC BACT4 MF-FAT for detection of Renibacterium salmoninarum (pdf)

Title: Membrane-Filtration Fluorescent Antibody Text (MF-FAT) for the detection of Renibacterium salmoninarum

Number: BACT-4

Version: 02 Created January 10, 2012

Purpose: Detection of Renibacterium salmoninarum in fish fluids or broth culture

DNA extraction of Renibacterium salmoninarum Thumb
February 26, 2020

WFRC BACT5 DNA extraction of Renibacterium salmoninarum (pdf)

Title: DNA Extraction for PCR-based Detection of Renibacterium salmoninarum

Number: BACT-5

Version: 01 Created April 15, 2011

Purpose: Recommended DNA extraction procedure of fish tissues and ovarian fluids for PCR-based detection of Renibacterium salmoninarum

Photo of scientist at loading gel Thumb
February 26, 2020

WFRC BACT6 nPCR test for detection of Renibacterium salmoninarum (pdf)

Title: Nested Polymerase Chain Reaction (nPCR) for the detection of Renibacterium salmoninarum

Number: BACT-6

Version: 01 Created April 15, 2011

Purpose: To detect nucleic acids from Renibacterium salmoninarum in DNA extracted from fish tissues or fluids using a nested PCR primer set

qPCR for detection of Rs 2Thumb
February 26, 2020

WFRC BACT7 qPCR for detection of Renibacterium salmoninarum (pdf)

Title: Quantitative Polymerase Chain Reaction (qPCR) for the detection of Renibacterium salmoninarum

Number: BACT-7

Version: 02 Created December 14, 2011

Purpose: To detect nucleic acids from Renibacterium salmoninarum in DNA extracted from fish tissues or fluids using quantitative PCR