Steffanie H Keefe (Former Employee)
Science and Products
Unique Approach to Measure Per- and Polyfluoroalkyl Substances Uptake in Fish, Mussels, and Passive Samplers
Per- and polyfluoroalkyl substances (PFAS) uptake and bioconcentration by fish and mussels ─ housed in mobile laboratories at a legacy fire-training area contaminated by aqueous film-forming foams ─ varied by species, sex, and compound. PFAS in passive samplers deployed at the same time mimicked uptake by fish but not mussels indicating that passive samplers might prove useful as screening tools...
Upper Colorado River Basin Accumulated Wastewater Ratios and PFAS Model Predictions (ver. 2.0, August 2025) Upper Colorado River Basin Accumulated Wastewater Ratios and PFAS Model Predictions (ver. 2.0, August 2025)
A wastewater reuse model was applied to the Upper Colorado River basin to assess the (1) percent of accumulated wastewater (ACCWW%) from municipal and industrial wastewater treatment plants (WWTPs) in each initialized (that is, has an assigned hydrosequence number for flow-routing) National Hydrography Dataset Version 2.1 (NHDPlus V2.1) stream segment; and (2) predicted environmental
Upper Colorado River Basin Accumulated Wastewater Ratios Upper Colorado River Basin Accumulated Wastewater Ratios
A wastewater reuse model was applied to the Upper Colorado River basin to assess the percent of accumulated wastewater (ACCWW%) from municipal and industrial wastewater treatment plants (WWTPs) in each initialized (that is, has an assigned hydrosequence number for flow-routing) National Hydrography Dataset Version 2.1 (NHDPlus V2.1) stream segment. Model inputs included (1) NHDPlus V2.1...
Uptake of Per- and Polyfluoroalkyl Substances by Fish, Mussel, and Passive Samplers in Mobile Laboratory Exposures using Groundwater from a Contamination Plume at a Historical Fire Training Area, Cape Cod, Massachusetts - Chemical and Biological Data from Uptake of Per- and Polyfluoroalkyl Substances by Fish, Mussel, and Passive Samplers in Mobile Laboratory Exposures using Groundwater from a Contamination Plume at a Historical Fire Training Area, Cape Cod, Massachusetts - Chemical and Biological Data from
This data release presents chemical and biological results from an investigation of the uptake of per- and polyfluoroalkyl substances (PFAS) from groundwater contaminated by fire training activities on Cape Cod, Massachusetts. Exposure experiments were conducted from August 29 to September 21, 2018 using groundwater from a relatively uncontaminated reference site and a fire training area
Potomac River Watershed Accumulated Wastewater Ratios and Predicted Environmental Concentrations Potomac River Watershed Accumulated Wastewater Ratios and Predicted Environmental Concentrations
Treated effluent from wastewater treatment plants (WWTPs) contains contaminants not fully removed during the treatment process and that may pose environmental health risks when discharged to surface waters. This data release presents inputs for and results from a wastewater reuse model that used data compiled from several sources to calculate the following estimates for each non-tidal...
Chemical Data From 40 Years of Monitoring a Treated-Wastewater Groundwater Plume in a Sand and Gravel Aquifer, Cape Cod, Massachusetts, 1978-2018 (ver. 1.1, April 2025) Chemical Data From 40 Years of Monitoring a Treated-Wastewater Groundwater Plume in a Sand and Gravel Aquifer, Cape Cod, Massachusetts, 1978-2018 (ver. 1.1, April 2025)
This U.S. Geological Survey data release provides a comprehensive dataset of water-quality data and sampling-site characteristics collected in 1978–2018 during a study of the effects of land disposal of treated wastewater on groundwater quality in an unconsolidated sand and gravel aquifer on Cape Cod, Massachusetts. Treated sewage-derived wastewater was discharged to rapid-infiltration...
Assessment of Endocrine Disruption in the Shenandoah River Watershed - Chemical and Biological Data from Mobile Laboratory Fish Exposures and Other Experiments Conducted during 2014, 2015, and 2016 Assessment of Endocrine Disruption in the Shenandoah River Watershed - Chemical and Biological Data from Mobile Laboratory Fish Exposures and Other Experiments Conducted during 2014, 2015, and 2016
This data release presents chemical and biological results from investigations of water quality, fish endocrine disruption, and emergent insects in the Shenandoah River Watershed (Virginia and West Virginia, USA) conducted during 2014, 2015, and 2016. Multiple sampling campaigns were conducted at sites located throughout the Shenandoah River Watershed (Table 1). The complex inorganic and...
Filter Total Items: 22
Assessment of per- and polyfluoroalkyl substances in water resources of New Mexico, 2020–21 Assessment of per- and polyfluoroalkyl substances in water resources of New Mexico, 2020–21
Per- and polyfluoroalkyl substances (PFAS) have been detected in public and private drinking-water wells, springs, and surface waters in New Mexico; however, the presence and distribution of PFAS in water resources across the State are not well characterized. From August 2020 to October 2021, the U.S. Geological Survey, in cooperation with the New Mexico Environment Department, collected...
Authors
Rebecca E. Travis, Kimberly R. Beisner, Kate Wilkins, Jeramy Roland Jasmann, Steffanie H. Keefe, Larry B. Barber
Uptake of per- and polyfluoroalkyl substances by fish, mussel, and passive samplers in mobile laboratory exposures using groundwater from a contamination plume at a historical fire training area, Cape Cod, Massachusetts Uptake of per- and polyfluoroalkyl substances by fish, mussel, and passive samplers in mobile laboratory exposures using groundwater from a contamination plume at a historical fire training area, Cape Cod, Massachusetts
Aqueous film-forming foams historically were used during fire training activities on Joint Base Cape Cod, Massachusetts, and created an extensive per- and polyfluoroalkyl substances (PFAS) groundwater contamination plume. The potential for PFAS bioconcentration from exposure to the contaminated groundwater, which discharges to surface water bodies, was assessed with mobile-laboratory...
Authors
Larry B. Barber, Heidi M. Pickard, David A. Alvarez, Jitka Becanova, Steffanie H. Keefe, Denis R. LeBlanc, Rainer Lohmann, Jeffery A. Steevens, Alan M. Vajda
Wastewater reuse and predicted ecological risk posed by contaminant mixtures in Potomac River watershed streams Wastewater reuse and predicted ecological risk posed by contaminant mixtures in Potomac River watershed streams
A wastewater model was applied to the Potomac River watershed to provide (i) a means to identify streams with a high likelihood of carrying elevated effluent-derived contaminants and (ii) risk assessments to aquatic life and drinking water. The model linked effluent discharges along stream networks, accumulated wastewater, and predicted contaminant loads of municipal wastewater...
Authors
Kaycee E. Faunce, Larry B. Barber, Steffanie H. Keefe, Jeramy Roland Jasmann, Jennifer L. Krstolic
Watershed-scale risk to aquatic organisms from complex chemical mixtures in the Shenandoah River Watershed-scale risk to aquatic organisms from complex chemical mixtures in the Shenandoah River
River waters contain complex chemical mixtures derived from natural and anthropogenic sources. Aquatic organisms are exposed to the entire chemical composition of the water, resulting in potential effects at the organismal through ecosystem level. This study applied a holistic approach to assess landscape, hydrological, chemical, and biological variables. On-site mobile laboratory...
Authors
Larry B. Barber, Kaycee E. Faunce, David Bertolatus, Michelle L. Hladik, Jeramy Roland Jasmann, Steffanie H. Keefe, Dana W. Kolpin, Michael T. Meyer, Jennifer L. Rapp, David A. Roth, Alan M. Vajda
Urban stormwater: An overlooked pathway of extensive mixed contaminants to surface and groundwaters in the United States Urban stormwater: An overlooked pathway of extensive mixed contaminants to surface and groundwaters in the United States
Increasing global reliance on stormwater control measures to reduce discharge to surface water, increase groundwater recharge, and minimize contaminant delivery to receiving waterbodies necessitates improved understanding of stormwater-contaminant profiles. A multi-agency study of organic and inorganic chemicals in urban stormwater from 50 runoff events at 21 sites across the United...
Authors
Jason R. Masoner, Dana W. Kolpin, Isabelle M. Cozzarelli, Larry B. Barber, D.S. Burden, William T. Foreman, Kenneth J. Forshay, Edward Furlong, Justin F. Groves, Michelle L. Hladik, Matthew E. Hopton, Jeanne B. Jaeschke, Steffanie H. Keefe, David Krabbenhoft, Richard Lowrance, Kristin Romanok, David L. Rus, William R. Selbig, Brad Williams, Paul Bradley
By
Water Resources Mission Area, Science Synthesis, Analysis, and Research Program, Contaminant Biology, Science Analytics and Synthesis (SAS) Program, Toxic Substances Hydrology, California Water Science Center, Central Midwest Water Science Center, New Jersey Water Science Center, Oklahoma-Texas Water Science Center, South Atlantic Water Science Center (SAWSC), Upper Midwest Environmental Sciences Center, Upper Midwest Water Science Center, Reston Biogeochemical Processes in Groundwater Laboratory
Integrated assessment of wastewater reuse, exposure risk, and fish endocrine disruption in the Shenandoah River watershed Integrated assessment of wastewater reuse, exposure risk, and fish endocrine disruption in the Shenandoah River watershed
Reuse of municipal and industrial wastewater treatment plant (WWTP) effluent is an important component in augmenting global freshwater supplies. The Shenandoah River Watershed was selected to conduct on-site exposure experiments to assess endocrine disrupting characteristics of different source waters. This investigation of the Shenandoah River Watershed integrates WWTP wastewater reuse...
Authors
Larry B. Barber, Jennifer L. Krstolic, Chintamani Kandel, Steffanie H. Keefe, Jacelyn Rice, Paul Westerhoff, David Bertolatus, Alan M. Vajda
Science and Products
Unique Approach to Measure Per- and Polyfluoroalkyl Substances Uptake in Fish, Mussels, and Passive Samplers
Per- and polyfluoroalkyl substances (PFAS) uptake and bioconcentration by fish and mussels ─ housed in mobile laboratories at a legacy fire-training area contaminated by aqueous film-forming foams ─ varied by species, sex, and compound. PFAS in passive samplers deployed at the same time mimicked uptake by fish but not mussels indicating that passive samplers might prove useful as screening tools...
Upper Colorado River Basin Accumulated Wastewater Ratios and PFAS Model Predictions (ver. 2.0, August 2025) Upper Colorado River Basin Accumulated Wastewater Ratios and PFAS Model Predictions (ver. 2.0, August 2025)
A wastewater reuse model was applied to the Upper Colorado River basin to assess the (1) percent of accumulated wastewater (ACCWW%) from municipal and industrial wastewater treatment plants (WWTPs) in each initialized (that is, has an assigned hydrosequence number for flow-routing) National Hydrography Dataset Version 2.1 (NHDPlus V2.1) stream segment; and (2) predicted environmental
Upper Colorado River Basin Accumulated Wastewater Ratios Upper Colorado River Basin Accumulated Wastewater Ratios
A wastewater reuse model was applied to the Upper Colorado River basin to assess the percent of accumulated wastewater (ACCWW%) from municipal and industrial wastewater treatment plants (WWTPs) in each initialized (that is, has an assigned hydrosequence number for flow-routing) National Hydrography Dataset Version 2.1 (NHDPlus V2.1) stream segment. Model inputs included (1) NHDPlus V2.1...
Uptake of Per- and Polyfluoroalkyl Substances by Fish, Mussel, and Passive Samplers in Mobile Laboratory Exposures using Groundwater from a Contamination Plume at a Historical Fire Training Area, Cape Cod, Massachusetts - Chemical and Biological Data from Uptake of Per- and Polyfluoroalkyl Substances by Fish, Mussel, and Passive Samplers in Mobile Laboratory Exposures using Groundwater from a Contamination Plume at a Historical Fire Training Area, Cape Cod, Massachusetts - Chemical and Biological Data from
This data release presents chemical and biological results from an investigation of the uptake of per- and polyfluoroalkyl substances (PFAS) from groundwater contaminated by fire training activities on Cape Cod, Massachusetts. Exposure experiments were conducted from August 29 to September 21, 2018 using groundwater from a relatively uncontaminated reference site and a fire training area
Potomac River Watershed Accumulated Wastewater Ratios and Predicted Environmental Concentrations Potomac River Watershed Accumulated Wastewater Ratios and Predicted Environmental Concentrations
Treated effluent from wastewater treatment plants (WWTPs) contains contaminants not fully removed during the treatment process and that may pose environmental health risks when discharged to surface waters. This data release presents inputs for and results from a wastewater reuse model that used data compiled from several sources to calculate the following estimates for each non-tidal...
Chemical Data From 40 Years of Monitoring a Treated-Wastewater Groundwater Plume in a Sand and Gravel Aquifer, Cape Cod, Massachusetts, 1978-2018 (ver. 1.1, April 2025) Chemical Data From 40 Years of Monitoring a Treated-Wastewater Groundwater Plume in a Sand and Gravel Aquifer, Cape Cod, Massachusetts, 1978-2018 (ver. 1.1, April 2025)
This U.S. Geological Survey data release provides a comprehensive dataset of water-quality data and sampling-site characteristics collected in 1978–2018 during a study of the effects of land disposal of treated wastewater on groundwater quality in an unconsolidated sand and gravel aquifer on Cape Cod, Massachusetts. Treated sewage-derived wastewater was discharged to rapid-infiltration...
Assessment of Endocrine Disruption in the Shenandoah River Watershed - Chemical and Biological Data from Mobile Laboratory Fish Exposures and Other Experiments Conducted during 2014, 2015, and 2016 Assessment of Endocrine Disruption in the Shenandoah River Watershed - Chemical and Biological Data from Mobile Laboratory Fish Exposures and Other Experiments Conducted during 2014, 2015, and 2016
This data release presents chemical and biological results from investigations of water quality, fish endocrine disruption, and emergent insects in the Shenandoah River Watershed (Virginia and West Virginia, USA) conducted during 2014, 2015, and 2016. Multiple sampling campaigns were conducted at sites located throughout the Shenandoah River Watershed (Table 1). The complex inorganic and...
Filter Total Items: 22
Assessment of per- and polyfluoroalkyl substances in water resources of New Mexico, 2020–21 Assessment of per- and polyfluoroalkyl substances in water resources of New Mexico, 2020–21
Per- and polyfluoroalkyl substances (PFAS) have been detected in public and private drinking-water wells, springs, and surface waters in New Mexico; however, the presence and distribution of PFAS in water resources across the State are not well characterized. From August 2020 to October 2021, the U.S. Geological Survey, in cooperation with the New Mexico Environment Department, collected...
Authors
Rebecca E. Travis, Kimberly R. Beisner, Kate Wilkins, Jeramy Roland Jasmann, Steffanie H. Keefe, Larry B. Barber
Uptake of per- and polyfluoroalkyl substances by fish, mussel, and passive samplers in mobile laboratory exposures using groundwater from a contamination plume at a historical fire training area, Cape Cod, Massachusetts Uptake of per- and polyfluoroalkyl substances by fish, mussel, and passive samplers in mobile laboratory exposures using groundwater from a contamination plume at a historical fire training area, Cape Cod, Massachusetts
Aqueous film-forming foams historically were used during fire training activities on Joint Base Cape Cod, Massachusetts, and created an extensive per- and polyfluoroalkyl substances (PFAS) groundwater contamination plume. The potential for PFAS bioconcentration from exposure to the contaminated groundwater, which discharges to surface water bodies, was assessed with mobile-laboratory...
Authors
Larry B. Barber, Heidi M. Pickard, David A. Alvarez, Jitka Becanova, Steffanie H. Keefe, Denis R. LeBlanc, Rainer Lohmann, Jeffery A. Steevens, Alan M. Vajda
Wastewater reuse and predicted ecological risk posed by contaminant mixtures in Potomac River watershed streams Wastewater reuse and predicted ecological risk posed by contaminant mixtures in Potomac River watershed streams
A wastewater model was applied to the Potomac River watershed to provide (i) a means to identify streams with a high likelihood of carrying elevated effluent-derived contaminants and (ii) risk assessments to aquatic life and drinking water. The model linked effluent discharges along stream networks, accumulated wastewater, and predicted contaminant loads of municipal wastewater...
Authors
Kaycee E. Faunce, Larry B. Barber, Steffanie H. Keefe, Jeramy Roland Jasmann, Jennifer L. Krstolic
Watershed-scale risk to aquatic organisms from complex chemical mixtures in the Shenandoah River Watershed-scale risk to aquatic organisms from complex chemical mixtures in the Shenandoah River
River waters contain complex chemical mixtures derived from natural and anthropogenic sources. Aquatic organisms are exposed to the entire chemical composition of the water, resulting in potential effects at the organismal through ecosystem level. This study applied a holistic approach to assess landscape, hydrological, chemical, and biological variables. On-site mobile laboratory...
Authors
Larry B. Barber, Kaycee E. Faunce, David Bertolatus, Michelle L. Hladik, Jeramy Roland Jasmann, Steffanie H. Keefe, Dana W. Kolpin, Michael T. Meyer, Jennifer L. Rapp, David A. Roth, Alan M. Vajda
Urban stormwater: An overlooked pathway of extensive mixed contaminants to surface and groundwaters in the United States Urban stormwater: An overlooked pathway of extensive mixed contaminants to surface and groundwaters in the United States
Increasing global reliance on stormwater control measures to reduce discharge to surface water, increase groundwater recharge, and minimize contaminant delivery to receiving waterbodies necessitates improved understanding of stormwater-contaminant profiles. A multi-agency study of organic and inorganic chemicals in urban stormwater from 50 runoff events at 21 sites across the United...
Authors
Jason R. Masoner, Dana W. Kolpin, Isabelle M. Cozzarelli, Larry B. Barber, D.S. Burden, William T. Foreman, Kenneth J. Forshay, Edward Furlong, Justin F. Groves, Michelle L. Hladik, Matthew E. Hopton, Jeanne B. Jaeschke, Steffanie H. Keefe, David Krabbenhoft, Richard Lowrance, Kristin Romanok, David L. Rus, William R. Selbig, Brad Williams, Paul Bradley
By
Water Resources Mission Area, Science Synthesis, Analysis, and Research Program, Contaminant Biology, Science Analytics and Synthesis (SAS) Program, Toxic Substances Hydrology, California Water Science Center, Central Midwest Water Science Center, New Jersey Water Science Center, Oklahoma-Texas Water Science Center, South Atlantic Water Science Center (SAWSC), Upper Midwest Environmental Sciences Center, Upper Midwest Water Science Center, Reston Biogeochemical Processes in Groundwater Laboratory
Integrated assessment of wastewater reuse, exposure risk, and fish endocrine disruption in the Shenandoah River watershed Integrated assessment of wastewater reuse, exposure risk, and fish endocrine disruption in the Shenandoah River watershed
Reuse of municipal and industrial wastewater treatment plant (WWTP) effluent is an important component in augmenting global freshwater supplies. The Shenandoah River Watershed was selected to conduct on-site exposure experiments to assess endocrine disrupting characteristics of different source waters. This investigation of the Shenandoah River Watershed integrates WWTP wastewater reuse...
Authors
Larry B. Barber, Jennifer L. Krstolic, Chintamani Kandel, Steffanie H. Keefe, Jacelyn Rice, Paul Westerhoff, David Bertolatus, Alan M. Vajda