Environmental DNA (eDNA) research at CERC focuses on the development and utility of eDNA tools as well as interpretation of eDNA data in real-world management applications. We work with academic, state, federal and international partners in developing standards and best practices for eDNA technology and exploring the factors that affect eDNA detection in the field. Our lab's eDNA research covers four main topics: (1) invasive species detection; (2) threatened, endangered, or rare species detection; (3) the ecology of eDNA; (4) eDNA applications for environmental toxicology.
Invasive species detection - Principal investigators: Cathy Richter and Katy Klymus
Detecting a non-native species early in its introduction into an ecosystem can increase the success of species removal efforts or improve the management of that species should it become invasive. Such early detection requires highly sensitive detection methods, and since the seminal work of Ficetola et al. (2008) demonstrating the use of eDNA for detection of invasive bullfrogs in Europe, eDNA has become a tool in the detection and surveillance of many other invasive species. Our lab works with biologists and wildlife managers on several projects involving invasive species detection with eDNA.
Threatened, endangered or rare species detection - Principal investigators: Cathy Richter and Katy Klymus
Threatened, endangered or rare species detection
The non-invasive nature of eDNA methods for species detection is a great advantage for monitoring of rare, threatened, or endangered species. Many traditional methods can result in stress to the target organisms and/or alterations to their habitat. Management questions such as definition of extant range, screening for appropriate sites for reintroductions, and monitoring changes in populations following restoration efforts, can all be addressed through eDNA analysis. Our lab studies rare species including the longnose darter, topeka shiner, spectaclecase mussel, and oyster mussel. Video: Darter capture by oyster mussel
Ecology of eDNA - Principal investigators: Cathy Richter and Katy Klymus
The ecology of eDNA is defined by Barnes and Turner (2016) as the interaction between the shed DNA of an organism and its environment. Understanding the factors that influence the origin or shedding of eDNA, the physical state of this DNA, the transport and detection of these molecules in a system and finally the degradation, decay or fate of DNA molecules will improve our ability to interpret eDNA data in the field. Our lab addresses these questions by running laboratory experiments to examine shedding and decay rates for various species. We also work in conjunction with the CERC River Studies hydrology and geomorphology group to examine the fate and detection of eDNA in various aquatic systems.
eDNA Applications for environmental toxicology - Principal investigators: Cathy Richter and Katy Klymus
Pairing eDNA methods for detection of wild organisms in the field with chemical detection of pollutants, testing of toxicity in the laboratory, and new technologies such as landscape use analysis, may lead to a more complete understanding of contaminant effects on fish and wildlife communities. Analysis of eDNA can be used to detect sensitive species, to study biodiversity in the field, and to assess ecosystem function (Zhang, 2019). Monitoring fish and wildlife populations with eDNA can help improve the quality of information gained in ecotoxicology studies and move beyond the single organism scope to community wide effects. Our lab uses metabarcoding to address questions about food web structure and species presence at potentially contaminated sites, including sites near uranium mining activity in the desert southwest United States (Klymus et al. 2017).
Return to Biochemistry and Physiology
Physical Stream Dynamics and Native Mussel Habitats
Below are data or web applications associated with this project.
Method comparison for the detection of environmental DNA from three species of unionid mussels in a mesocosm Method comparison for the detection of environmental DNA from three species of unionid mussels in a mesocosm
Detection of Ortmanniana ligamentina male mitotype DNA from a mesocosm during a natural spawning event Detection of Ortmanniana ligamentina male mitotype DNA from a mesocosm during a natural spawning event
eDNA shedding rates for juvenile and subadult black carp fed a commercial diet or a Corbicula mussel diet in a laboratory setting eDNA shedding rates for juvenile and subadult black carp fed a commercial diet or a Corbicula mussel diet in a laboratory setting
Detection of Cumberlandia monodonta male mitotype eDNA from an outdoor raceway tank during a spawning event Detection of Cumberlandia monodonta male mitotype eDNA from an outdoor raceway tank during a spawning event
Detection of exogenous lake trout and endogenous spectaclecase DNA in the Big Piney River from an experimental release of lake trout slurry to study eDNA transport, September 2023 (ver.1.1, May 2025) Detection of exogenous lake trout and endogenous spectaclecase DNA in the Big Piney River from an experimental release of lake trout slurry to study eDNA transport, September 2023 (ver.1.1, May 2025)
Environmental DNA qPCR detection results and covariates from Idaho, Missouri, Montana, and New York rivers sampled in 2023 Environmental DNA qPCR detection results and covariates from Idaho, Missouri, Montana, and New York rivers sampled in 2023
Metabarcoding of environmental DNA samples collected below Jackson Lake Dam (Wyoming) and Palisades Reservoir (Idaho) in 2021-2022 (ver. 2.0, November 2024) Metabarcoding of environmental DNA samples collected below Jackson Lake Dam (Wyoming) and Palisades Reservoir (Idaho) in 2021-2022 (ver. 2.0, November 2024)
Environmental DNA (eDNA) Metabarcoding assessment of dead-end hollow fiber ultrafiltration (D-HFUF) and polyethylstyrene (PES) filters filtration methods on detection of freshwater mussel eDNA from Flint River and Spring Creek, Georgia and Big Piney River Environmental DNA (eDNA) Metabarcoding assessment of dead-end hollow fiber ultrafiltration (D-HFUF) and polyethylstyrene (PES) filters filtration methods on detection of freshwater mussel eDNA from Flint River and Spring Creek, Georgia and Big Piney River
Laboratory estimates of eDNA degradation rates for Actinionais ligamentina eDNA Laboratory estimates of eDNA degradation rates for Actinionais ligamentina eDNA
Development and testing of a qPCR assay for Lampsilis siliquoidea eDNA Development and testing of a qPCR assay for Lampsilis siliquoidea eDNA
Development, testing and use of four species-specific qPCR assays for freshwater mussel eDNA detection in the Niobrara River Development, testing and use of four species-specific qPCR assays for freshwater mussel eDNA detection in the Niobrara River
Environmental DNA shedding rates in laboratory conditions for Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea. Environmental DNA shedding rates in laboratory conditions for Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea.
Below are publications associated with this project.
What is eDNA method standardization and why do we need it? What is eDNA method standardization and why do we need it?
The MIEM guidelines: Minimum information for reporting of environmental metabarcoding data The MIEM guidelines: Minimum information for reporting of environmental metabarcoding data
Field trials of an autonomous eDNA sampler in lotic waters Field trials of an autonomous eDNA sampler in lotic waters
Detection and transport of environmental DNA from two federally endangered mussels Detection and transport of environmental DNA from two federally endangered mussels
Environmental DNA dynamics of three species of unionid freshwater mussels Environmental DNA dynamics of three species of unionid freshwater mussels
Dead-end hollow fiber ultrafiltration capture of environmental DNA for freshwater mussel (Unionidae) species detection with metabarcoding Dead-end hollow fiber ultrafiltration capture of environmental DNA for freshwater mussel (Unionidae) species detection with metabarcoding
A nitrifier-enriched microbial community contributes to the degradation of environmental DNA A nitrifier-enriched microbial community contributes to the degradation of environmental DNA
Gaining decision-maker confidence through community consensus: Developing environmental DNA standards for data display on the USGS Nonindigenous Aquatic Species database Gaining decision-maker confidence through community consensus: Developing environmental DNA standards for data display on the USGS Nonindigenous Aquatic Species database
Editorial: Environmental DNA innovations for conservation Editorial: Environmental DNA innovations for conservation
Metabarcoding assays for the detection of freshwater mussels (Unionida) with environmental DNA Metabarcoding assays for the detection of freshwater mussels (Unionida) with environmental DNA
Development and testing of species-specific quantitative PCR assays for environmental DNA applications Development and testing of species-specific quantitative PCR assays for environmental DNA applications
Reporting the limits of detection and quantification for environmental DNA assays Reporting the limits of detection and quantification for environmental DNA assays
Environmental DNA (eDNA) research at CERC focuses on the development and utility of eDNA tools as well as interpretation of eDNA data in real-world management applications. We work with academic, state, federal and international partners in developing standards and best practices for eDNA technology and exploring the factors that affect eDNA detection in the field. Our lab's eDNA research covers four main topics: (1) invasive species detection; (2) threatened, endangered, or rare species detection; (3) the ecology of eDNA; (4) eDNA applications for environmental toxicology.
Invasive species detection - Principal investigators: Cathy Richter and Katy Klymus
Detecting a non-native species early in its introduction into an ecosystem can increase the success of species removal efforts or improve the management of that species should it become invasive. Such early detection requires highly sensitive detection methods, and since the seminal work of Ficetola et al. (2008) demonstrating the use of eDNA for detection of invasive bullfrogs in Europe, eDNA has become a tool in the detection and surveillance of many other invasive species. Our lab works with biologists and wildlife managers on several projects involving invasive species detection with eDNA.
Threatened, endangered or rare species detection - Principal investigators: Cathy Richter and Katy Klymus
Threatened, endangered or rare species detection
The non-invasive nature of eDNA methods for species detection is a great advantage for monitoring of rare, threatened, or endangered species. Many traditional methods can result in stress to the target organisms and/or alterations to their habitat. Management questions such as definition of extant range, screening for appropriate sites for reintroductions, and monitoring changes in populations following restoration efforts, can all be addressed through eDNA analysis. Our lab studies rare species including the longnose darter, topeka shiner, spectaclecase mussel, and oyster mussel. Video: Darter capture by oyster mussel
Ecology of eDNA - Principal investigators: Cathy Richter and Katy Klymus
The ecology of eDNA is defined by Barnes and Turner (2016) as the interaction between the shed DNA of an organism and its environment. Understanding the factors that influence the origin or shedding of eDNA, the physical state of this DNA, the transport and detection of these molecules in a system and finally the degradation, decay or fate of DNA molecules will improve our ability to interpret eDNA data in the field. Our lab addresses these questions by running laboratory experiments to examine shedding and decay rates for various species. We also work in conjunction with the CERC River Studies hydrology and geomorphology group to examine the fate and detection of eDNA in various aquatic systems.
eDNA Applications for environmental toxicology - Principal investigators: Cathy Richter and Katy Klymus
Pairing eDNA methods for detection of wild organisms in the field with chemical detection of pollutants, testing of toxicity in the laboratory, and new technologies such as landscape use analysis, may lead to a more complete understanding of contaminant effects on fish and wildlife communities. Analysis of eDNA can be used to detect sensitive species, to study biodiversity in the field, and to assess ecosystem function (Zhang, 2019). Monitoring fish and wildlife populations with eDNA can help improve the quality of information gained in ecotoxicology studies and move beyond the single organism scope to community wide effects. Our lab uses metabarcoding to address questions about food web structure and species presence at potentially contaminated sites, including sites near uranium mining activity in the desert southwest United States (Klymus et al. 2017).
Return to Biochemistry and Physiology
Physical Stream Dynamics and Native Mussel Habitats
Below are data or web applications associated with this project.
Method comparison for the detection of environmental DNA from three species of unionid mussels in a mesocosm Method comparison for the detection of environmental DNA from three species of unionid mussels in a mesocosm
Detection of Ortmanniana ligamentina male mitotype DNA from a mesocosm during a natural spawning event Detection of Ortmanniana ligamentina male mitotype DNA from a mesocosm during a natural spawning event
eDNA shedding rates for juvenile and subadult black carp fed a commercial diet or a Corbicula mussel diet in a laboratory setting eDNA shedding rates for juvenile and subadult black carp fed a commercial diet or a Corbicula mussel diet in a laboratory setting
Detection of Cumberlandia monodonta male mitotype eDNA from an outdoor raceway tank during a spawning event Detection of Cumberlandia monodonta male mitotype eDNA from an outdoor raceway tank during a spawning event
Detection of exogenous lake trout and endogenous spectaclecase DNA in the Big Piney River from an experimental release of lake trout slurry to study eDNA transport, September 2023 (ver.1.1, May 2025) Detection of exogenous lake trout and endogenous spectaclecase DNA in the Big Piney River from an experimental release of lake trout slurry to study eDNA transport, September 2023 (ver.1.1, May 2025)
Environmental DNA qPCR detection results and covariates from Idaho, Missouri, Montana, and New York rivers sampled in 2023 Environmental DNA qPCR detection results and covariates from Idaho, Missouri, Montana, and New York rivers sampled in 2023
Metabarcoding of environmental DNA samples collected below Jackson Lake Dam (Wyoming) and Palisades Reservoir (Idaho) in 2021-2022 (ver. 2.0, November 2024) Metabarcoding of environmental DNA samples collected below Jackson Lake Dam (Wyoming) and Palisades Reservoir (Idaho) in 2021-2022 (ver. 2.0, November 2024)
Environmental DNA (eDNA) Metabarcoding assessment of dead-end hollow fiber ultrafiltration (D-HFUF) and polyethylstyrene (PES) filters filtration methods on detection of freshwater mussel eDNA from Flint River and Spring Creek, Georgia and Big Piney River Environmental DNA (eDNA) Metabarcoding assessment of dead-end hollow fiber ultrafiltration (D-HFUF) and polyethylstyrene (PES) filters filtration methods on detection of freshwater mussel eDNA from Flint River and Spring Creek, Georgia and Big Piney River
Laboratory estimates of eDNA degradation rates for Actinionais ligamentina eDNA Laboratory estimates of eDNA degradation rates for Actinionais ligamentina eDNA
Development and testing of a qPCR assay for Lampsilis siliquoidea eDNA Development and testing of a qPCR assay for Lampsilis siliquoidea eDNA
Development, testing and use of four species-specific qPCR assays for freshwater mussel eDNA detection in the Niobrara River Development, testing and use of four species-specific qPCR assays for freshwater mussel eDNA detection in the Niobrara River
Environmental DNA shedding rates in laboratory conditions for Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea. Environmental DNA shedding rates in laboratory conditions for Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea.
Below are publications associated with this project.