Elliott Barnhart is a research hydrologist (microbiologist) at the Wyoming Montana Water Science Center in Helena, Montana. His research focuses on using new bioinformatics technology to better understand ecological processes, promote energy development and examine environmental issues.
Elliott's research specifically focuses on (1) understanding how subsurface microorganisms generate and consume methane, (2) developing and testing new DNA sampling and analytical equipment, and (3) investigating the prevalence of microorganisms, pathogens and invasive species in different ecosystems.
Science and Products
READI-Net: A network of robotic environmental DNA samplers to enhance the early detection of aquatic biological threats
Environmental DNA (eDNA): Combining Technology and Biology to Detect Aquatic Invasive Species and Pathogens
Using Robots in the River: Biosurveillance at USGS streamgages
High-Resolution, Interagency Biosurveillance of Threatened Surface Waters in the United States
Microbial Methanogenesis and Strategies for Enhancements
Chemistry Data from the Birney Test Site, Montana, 2018-2020
Injection of Deuterium and Yeast Extract at USGS Birney Field Site, Powder River Basin, Montana, USA, 2016-2020
Data for Biogeochemical and Physical Processes Controlling Mercury and Selenium Bioaccumulation in Bighorn Lake, Bighorn Canyon National Recreation Area, Montana and Wyoming, 2015-2016
A global perspective on bacterial diversity in the terrestrial deep subsurface
Methanogenic archaea in subsurface coal seams are biogeographically distinct: An analysis of metagenomically-derived mcrA sequences
Subsurface hydrocarbon degradation strategies in low- and high-sulfate coal seam communities identified with activity-based metagenomics
In situ enhancement and isotopic labeling of biogenic coalbed methane
Activity-based, genome-resolved metagenomics uncovers key populations and pathways involved in subsurface conversions of coal to methane
Effect of an algal amendment on the microbial conversion of coal to methane at different sulfate concentrations from the Powder River Basin, USA
Integrating environmental DNA results with diverse data sets to improve biosurveillance of river health
Effect of temperature, nitrate concentration, pH and bicarbonate addition on biomass and lipid accumulation in the sporulating green alga PW95
Robotic environmental DNA bio-surveillance of freshwater health
Repetitive sampling and control threshold improve 16S rRNA results from produced waters associated with hydraulically fractured shales
Coal biomethanation potential of various ranks from Pakistan: A possible alternative energy source
Biogenic coal-to-methane conversion can be enhanced with small additions of algal amendment in field-relevant upflow column reactors
Non-USGS Publications**
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Science and Products
- Science
READI-Net: A network of robotic environmental DNA samplers to enhance the early detection of aquatic biological threats
USGS researchers are working with the Monterey Bay Aquarium Research Institute to optimize autonomous, robotic samplers for detection of DNA fragments shed by biological threats (BT; invasive species, parasites, pathogens) in our nation’s waters. Finding DNA fragments (a method known as environmental DNA sampling) produced by an emerging BT in water is akin to finding a needle in a haystack—many...Environmental DNA (eDNA): Combining Technology and Biology to Detect Aquatic Invasive Species and Pathogens
Using DNA, USGS researchers are able to detect the presence of invasive species in aquatic ecosystems. The DNA they use is literally floating around in the environment and is called environmental DNA (eDNA) and is a powerful tool for the early detection of invasive species and pathogens, which can cause serious ecological and economic damage. USGS researchers are also combining the use of eDNA...Using Robots in the River: Biosurveillance at USGS streamgages
For more than a decade, researchers around the world have shown that sampling a water body and analyzing for DNA (a method known as eDNA) is an effective method to detect an organism in the water. The challenge is that finding organisms that are not very abundant requires a lot of samples to locate this needle in a haystack. Enter the "lab in a can", the water quality sampling and processing robot...High-Resolution, Interagency Biosurveillance of Threatened Surface Waters in the United States
Advances in information technology now provide large volume, high-frequency data collection which may improve real-time biosurveillance and forecasting. But, big data streams present challenges for data management and timely analysis. As a first step in creating a data science pipeline for translating large datasets into meaningful interpretations, we created a cloud-hosted PostgreSQL database thaMicrobial Methanogenesis and Strategies for Enhancements
Microbial (biogenic) natural gas is present in shale, coal and petroleum reservoirs and is estimated to account for 20% of the world’s natural gas resources. We provide hydrological, geochemical and microbial information related to the production of biogenic natural gas and new methods to monitor and enhance the production of this energy resource. Generating microbial methane at a faster rate from... - Data
Chemistry Data from the Birney Test Site, Montana, 2018-2020
Data were collected to monitor geochemistry before and after an injection designed to stimulate microbial methanogenesis in the shallow Flowers-Goodale coal bed, near Birney in southeastern Montana. Waters from wells completed in the Flowers-Goodale, Nance, Knobloch, and Terret coalbeds at the Birney Test Site were sampled. Geochemical characterization of the water included non-purgeable dissolvedInjection of Deuterium and Yeast Extract at USGS Birney Field Site, Powder River Basin, Montana, USA, 2016-2020
Subsurface microbial (biogenic) methane production is an important part of the global carbon cycle and has resulted in natural gas accumulations in many coal beds worldwide. Laboratory experiments indicate coal beds can act as natural geobioreactors and produce additional low carbon renewable natural gas with algal or yeast compounds, yet the effectiveness of these nutrients in situ are unknown. TData for Biogeochemical and Physical Processes Controlling Mercury and Selenium Bioaccumulation in Bighorn Lake, Bighorn Canyon National Recreation Area, Montana and Wyoming, 2015-2016
This dataset includes the field measurements and laboratory analyses of surface water, seston, fish tissue, and sediment samples collected from Bighorn Lake, within Bighorn Canyon National Recreation area (BICA), during high flow (July 2015) and low flow (August 2016) conditions. The study area includes 7-9 sampling sites that follow a transect spanning the entire length of the reservoir from the - Multimedia
- Publications
Filter Total Items: 24
A global perspective on bacterial diversity in the terrestrial deep subsurface
While recent efforts to catalogue Earth’s microbial diversity have focused upon surface and marine habitats, 12–20 % of Earth’s biomass is suggested to exist in the terrestrial deep subsurface, compared to ~1.8 % in the deep subseafloor. Metagenomic studies of the terrestrial deep subsurface have yielded a trove of divergent and functionally important microbiomes from a range of localities. HoweveMethanogenic archaea in subsurface coal seams are biogeographically distinct: An analysis of metagenomically-derived mcrA sequences
The production of methane as an end-product of organic matter degradation in the absence of other terminal electron acceptors is common, and has often been studied in environments such as animal guts, soils, and wetlands due to its potency as a greenhouse gas. To date however, the study of the biogeographic distribution of methanogens across coal seam environments has been minimal. Here, we show tSubsurface hydrocarbon degradation strategies in low- and high-sulfate coal seam communities identified with activity-based metagenomics
Environmentally relevant metagenomes and BONCAT-FACS derived translationally active metagenomes from Powder River Basin coal seams were investigated to elucidate potential genes and functional groups involved in hydrocarbon degradation to methane in coal seams with high- and low-sulfate levels. An advanced subsurface environmental sampler allowed the establishment of coal-associated microbial commIn situ enhancement and isotopic labeling of biogenic coalbed methane
Subsurface microbial (biogenic) methane production is an important part of the global carbon cycle that has resulted in natural gas accumulations in many coal beds worldwide. Laboratory studies suggest that complex carbon-containing nutrients (e.g., yeast or algae extract) can stimulate methane production, yet the effectiveness of these nutrients within coal beds is unknown. Here, we use downholeActivity-based, genome-resolved metagenomics uncovers key populations and pathways involved in subsurface conversions of coal to methane
Microbial metabolisms and interactions that facilitate subsurface conversions of recalcitrant carbon to methane are poorly understood. We deployed an in situ enrichment device in a subsurface coal seam in the Powder River Basin (PRB), USA, and used BONCAT-FACS-Metagenomics to identify translationally active populations involved in methane generation from a variety of coal-derived aromatic hydrocarEffect of an algal amendment on the microbial conversion of coal to methane at different sulfate concentrations from the Powder River Basin, USA
Biogenic methane is estimated to account for one-fifth of the natural gas worldwide and there is great interest in controlling methane from different sources. Biogenic coalbed methane (CBM) production relies on syntrophic associations between fermentative bacteria and methanogenic archaea to anaerobically degrade recalcitrant coal and produce methanogenic substrates. However, very little is knownIntegrating environmental DNA results with diverse data sets to improve biosurveillance of river health
Autonomous, robotic environmental (e)DNA samplers now make it possible for biological observations to match the scale and quality of abiotic measurements collected by automated sensor networks. Merging these automated data streams may allow for improved insight into biotic responses to environmental change and stressors. Here, we merged eDNA data collected by robotic samplers installed at three U.Effect of temperature, nitrate concentration, pH and bicarbonate addition on biomass and lipid accumulation in the sporulating green alga PW95
The mixed effects of temperature (20 °C, 25 °C and 30 °C), nitrate concentration (0.5 mM and 2.0 mM), pH buffer, and bicarbonate addition (trigger) on biomass growth and lipid accumulation were investigated in the environmental alga PW95 during batch experiments in standardized growth medium. PW95 was isolated from coal-bed methane production water and classified as a Chlamydomonas-like species byRobotic environmental DNA bio-surveillance of freshwater health
Autonomous water sampling technologies may help to overcome the human resource challenges of monitoring biological threats to rivers over long time periods and large geographic areas. The Monterey Bay Aquarium Research Institute has pioneered a robotic Environmental Sample Processor (ESP) that overcomes some of the constraints associated with traditional sampling since it can automate water sampleRepetitive sampling and control threshold improve 16S rRNA results from produced waters associated with hydraulically fractured shales
Sequencing microbial DNA from deep subsurface environments is complicated by a number of issues ranging from contamination to non-reproducible results. Many samples obtained from these environments - which are of great interest due to the potential to stimulate microbial methane generation - contain low biomass. Therefore, samples from these environments are difficult to study as sequencing resultCoal biomethanation potential of various ranks from Pakistan: A possible alternative energy source
The present study investigated the possibility of microbial transformations of coal to gas (biogasification) as an alternative to conventional coal mining because this approach has the potential to be less expensive, cleaner, and providinge greater access to deeper coal resources. Biogasification is often associated with low rank coal such as lignite and subbituminous coal that hasve produced enouBiogenic coal-to-methane conversion can be enhanced with small additions of algal amendment in field-relevant upflow column reactors
ubsurface coal environments, where biogenic coal-to-methane conversion occurs, are difficult to access, resulting in inherent challenges and expenses for in situexperiments. Previous batch reactor studies provided insights into specific processes, pathways, kinetics, and engineering strategies, but field-relevance is restricted due to limited substrate availability or byproduct accumulation that mNon-USGS Publications**
A. Soares, A. Edwards, D. An, A. Bagnoud, J. Bradley, E. Barnhart, M. Bomberg, K. Budwill, S.M. Caffrey, M. Fields, J. Gralnick, V. Kadnikov, L. Momper, M. Osburn, A. Mu, J.W. Moreau, D. Moser, L. Purkamo, S.M. Rassner, C. S. Sheik, B. Sherwood Lollar, B. M. Toner, G. Voordouw, K. Wouters, A.C. Mitchell (2023), A global perspective on bacterial diversity in the terrestrial deep subsurface: Microbiology Society, 169, doi.org/10.1099/mic.0.001172.**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
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