My research is focused on characterizing how microbes influence the geochemistry and carbon and nutrient cycling in surface water, ground water and coastal marine water and associated sediment systems thru the application of phylogenetics, microbial energetics and stable isotopes and radiolabeled substrates.
Professional Experience
2004-present Microbial Ecologist, USGS Center for Coastal & Watershed Studies, St. Petersburg, FL
2002-present Affiliate Graduate Faculty in the University of South Florida’s Biology Department
2002-2009 Assistant Courtesy Professor in the University of South Florida’s College of Marine Sciences
2002-2004 USGS Mendenhall Fellow, Center for Coastal & Watershed Studies, St. Petersburg, FL
2001-2002 Microbial Ecologist, NASA, Astrobiology Institute for the Study of Biomarkers, Johnson Space Center, Houston
2000-2001 Research Microbiologist, Lockheed Martin, NASA Astrobiology Institute for Biomarkers, Johnson Space Center, Houston
1998-2000 Assistant Research Professor, Department of Microbiology, Montana State University
1996-1998 Post-doctoral research fellow at Montana State University/Dr. Gordon McFeters, Department of Microbiology
Education and Certifications
1996 Ph.D., University of South Florida, College of Public Health
1983 M.S., Eastern Kentucky University, Department of Biology
1978 B.S., Eastern Kentucky University, Department of Biology
Affiliations and Memberships*
Collaborating scientist (2005-2007). The influence on microbial activities on arsenic mobilization from Floridan aquifer material (Dr. John Arthur/FLDEP).
Collaborating scientist (2005-2007). Microbial chemotaxis in hydrocarbon contaminated aquifers (Dr. Ron Harvey/USGS NRP).
Collaborating scientist (2004-Present). Characterization of bacterial and bacteriophage dynamics in ice and selected glacial melt streams and permanently ice-covered lakes in the Antarctic Dry Valleys
Science and Products
Target-Chemical Concentrations and Microbiological Results for Assessment of Mixed Contaminant and Biological Exposures in Bottled Water, 2020
Microbial Nutrient Cycling in the Upper Floridan Aquifer
Bottled water contaminant exposures and potential human effects
Groundwater chemistry, hydrogeologic properties, bioremediation potential, and three-dimensional numerical simulation of the sand and gravel aquifer at Naval Air Station Whiting Field, near Milton, Florida, 2015–20
Natural inactivation of MS2, poliovirus type 1 and Cryptosporidium parvum in an anaerobic and reduced aquifer
Nutrient removal and uptake by native planktonic and biofilm bacterial communities in an anaerobic aquifer
Colony-forming unit spreadplate assay versus liquid culture enrichment-polymerase chain reaction assay for the detection of Bacillus Endospores in soils
Spatial and temporal variability of pCO2, carbon fluxes and saturation state on the West Florida Shelf
Regional acidification trends in Florida shellfish estuaries: A 20+ year look at pH, oxygen, temperature, and salinity
Microbial formation of labile organic carbon in Antarctic glacial environments
Optimization of a sample processing protocol for recovery of Bacillus anthracis spores from soil
Natural inactivation of Escherichia coli in anoxic and reduced groundwater
Viral lysis of photosynthesizing microbes as a mechanism for calcium carbonate nucleation in seawater
USGS Arctic Ocean Carbon Cruise 2012: Field Activity L-01-12-AR to collect carbon data in the Arctic Ocean, August-September 2012
Collaborative Science Provides Understanding of Contaminants in Bottled Water-an Increasingly Common Alternate Drinking Water Source
Science and Products
- Data
Target-Chemical Concentrations and Microbiological Results for Assessment of Mixed Contaminant and Biological Exposures in Bottled Water, 2020
This data release contains concentration and quality-assurance results for inorganic, organic analytes and microbiological pathogens collected from 30 different commercial bottled water sources. Samples were processed on July 20 and August 17, 2020 at the New Jersey Water Science Center and analyzed at various U.S. Geological Survey laboratories. Samples were analyzed for nutrients, cations and anMicrobial Nutrient Cycling in the Upper Floridan Aquifer
Groundwater samples were collected from a South Florida Water Management District (SFWMD) monitoring well at the discharge point of the Kissimmee River into Lake Okeechobee between 2018 and 2019. The groundwater source was the Upper Floridan Aquifer at approximately 1,000 feet below land surface (fbls). Samples were dosed with nitrogen, phosphorus, and carbon, then sampled over a period of time to - Multimedia
- Publications
Filter Total Items: 36
Bottled water contaminant exposures and potential human effects
Bottled water (BW) consumption in the United States and globally has increased amidst heightened concern about environmental contaminant exposures and health risks in drinking water supplies, despite a paucity of directly comparable, environmentally-relevant contaminant exposure data for BW. This study provides insight into exposures and cumulative risks to human health from inorganic/organic/micrAuthorsPaul Bradley, Kristin Romanok, Kelly Smalling, Michael J. Focazio, Nicola Evans, Suzanne C. Fitzpatrick, Carrie E Givens, Stephanie Gordon, James L. Gray, Emily M. Green, Dale W. Griffin, Michelle Hladik, Leslie K. Kanagy, John T. Lisle, Keith Loftin, R. Blaine McCleskey, Elizabeth Medlock-Kakaley, Ana Navas-Acien, David A. Roth, Paul F. South, Christopher P. WeisGroundwater chemistry, hydrogeologic properties, bioremediation potential, and three-dimensional numerical simulation of the sand and gravel aquifer at Naval Air Station Whiting Field, near Milton, Florida, 2015–20
The U.S. Geological Survey completed a study between 2015 and 2020 of groundwater contamination in the sand and gravel aquifer at a Superfund site in northwestern Florida. Groundwater-quality samples were collected from representative monitoring wells located along a groundwater-flow pathway and analyzed in the field and laboratory. In general, ambient groundwater in the sand and gravel aquifer isAuthorsJames Landmeyer, Eric D. Swain, Carole D. Johnson, John T. Lisle, W. Scott McBride, David H. Chung, Michael A. SingletaryNatural inactivation of MS2, poliovirus type 1 and Cryptosporidium parvum in an anaerobic and reduced aquifer
AimsThe study of microbial inactivation rates in aquifer systems has most often been determined in aerobic and oxidized systems. This study examined the inactivation (i.e. loss of infectivity) of MS2, poliovirus type 1 (PV1) and Cryptosporidium parvum in an anaerobic and reduced groundwater system that has been identified as storage zones for aquifer storage and recovery (ASR) facilities.Methods aAuthorsJohn T. Lisle, Geroge LukasicNutrient removal and uptake by native planktonic and biofilm bacterial communities in an anaerobic aquifer
Managed aquifer recharge (MAR) offers a collection of water storage and storage options that have been used by resource managers to mitigate the reduced availability of fresh water. One of these technologies is aquifer storage and recovery (ASR), where surface water is treated then recharged into a storage zone within an existing aquifer for later recovery and discharge into a body of water. DurinAuthorsJohn T. LisleColony-forming unit spreadplate assay versus liquid culture enrichment-polymerase chain reaction assay for the detection of Bacillus Endospores in soils
A liquid culture enrichment-polymerase chain reaction (E-PCR) assay was investigated as a potential tool to overcome inhibition by chemical component, debris, and background biological impurities in soil that were affecting detection assay performance for soil samples containing Bacillus atrophaeus subsp. globigii (a surrogate for B. anthracis). To evaluate this assay, 9 g of matched sets of threeAuthorsDale W. Griffin, John T. Lisle, David Feldhake, Erin E. SilvestriSpatial and temporal variability of pCO2, carbon fluxes and saturation state on the West Florida Shelf
The West Florida Shelf (WFS) is a source of uncertainty for the Gulf of Mexico carbon budget. Data from the synthesis of approximately 135,000 pCO2 values from over 96 cruises from the WFS show that the shelf waters fluctuate between being a weak source to a weak sink of carbon with the atmosphere. Overall, the shelf acts as a weak source of CO2 at 0.32 ± 1.5 mol m-2 yr-1. Subregions, however, rAuthorsL. Robbins, K. Daley, L. Barbero, R. Wanninkhof, R.L. Heathcote, H. Zong, John T. Lisle, W.-J. Cai, C. SmithRegional acidification trends in Florida shellfish estuaries: A 20+ year look at pH, oxygen, temperature, and salinity
Increasing global CO2 and local land use changes coupled with increased nutrient pollution are threatening estuaries worldwide. Local changes of estuarine chemistry have been documented, but regional associations and trends comparing multiple estuaries latitudinally have not been evaluated. Rapid climate change has impacted the annual and decadal chemical trends in estuaries, with local ecosystemAuthorsLisa L. Robbins, John T. LisleMicrobial formation of labile organic carbon in Antarctic glacial environments
Roughly six petagrams of organic carbon are stored within ice worldwide. This organic carbon is thought to be of old age and highly bioavailable. Along with storage of ancient and new atmospherically deposited organic carbon, microorganisms may contribute substantially to the glacial organic carbon pool. Models of glacial microbial carbon cycling vary from net respiration to net carbon fixation. SAuthorsH.J. Smith, R. Foster, D.M. McKnight, John T. Lisle, S. Littmann, M.M.M. Kuypers, C.M. ForemanOptimization of a sample processing protocol for recovery of Bacillus anthracis spores from soil
Following a release of Bacillus anthracis spores into the environment, there is a potential for lasting environmental contamination in soils. There is a need for detection protocols for B. anthracis in environmental matrices. However, identification of B. anthracis within a soil is a difficult task. Processing soil samples helps to remove debris, chemical components, and biological impurities thatAuthorsErin E. Silvestri, David Feldhake, Dale Griffin, John T. Lisle, Tonya L. Nichols, Sanjiv Shah, A Pemberton, Frank W Schaefer IIINatural inactivation of Escherichia coli in anoxic and reduced groundwater
Aims Inactivation rates of E. coli in groundwater have most often been determined in aerobic and oxidized systems. This study examined E. coli inactivation rates in anaerobic and extremely reduced groundwater systems that have been identified as recharge zones. Methods and Results Groundwater from six artesian wells was diverted to above ground, flow through mesocosms that contained laboratory groAuthorsJohn T. LisleViral lysis of photosynthesizing microbes as a mechanism for calcium carbonate nucleation in seawater
Removal of carbon through the precipitation and burial of calcium carbonate in marine sediments constitutes over 70% of the total carbon on Earth and is partitioned between coastal and pelagic zones. The precipitation of authigenic calcium carbonate in seawater, however, has been hotly debated because despite being in a supersaturated state, there is an absence of persistent precipitation. One ofAuthorsJohn T. Lisle, Lisa L. RobbinsUSGS Arctic Ocean Carbon Cruise 2012: Field Activity L-01-12-AR to collect carbon data in the Arctic Ocean, August-September 2012
From August 25 to September 27, 2012, the United States Coast Guard Cutter (USCGC) Healy was part of an Extended Continental Shelf Project to determine the limits of the extended continental shelf in the Arctic. On a non-interference basis, a USGS ocean acidification team participated on the cruise to collect baseline water data in the Arctic. The collection of data extended from coastal waters neAuthorsLisa L. Robbins, Jonathan Wynn, Paul O. Knorr, Bogdan Onac, John T. Lisle, Katherine Y. McMullen, Kimberly K. Yates, Robert H. Byrne, Xuewu Liu - News
- Science
Collaborative Science Provides Understanding of Contaminants in Bottled Water-an Increasingly Common Alternate Drinking Water Source
U.S. Geological Survey researchers and public health experts collaborated to determine what contaminants occur in bottled water, which is an increasingly common alternate drinking water source, to broaden their understanding of human exposure to contaminants in drinking water supply chains. Bottled water, like public-supply and private-well tap water supply chains, contained multiple organic...
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government