Luke R Iwanowicz, Ph.D. (Former Employee)
Science and Products
Filter Total Items: 12
Prevalence of novel, emerging hepatitis viruses in wild white suckers and their association with liver tumors in the Great Lakes region
Prevalence of novel, emerging hepatitis viruses in wild white suckers and their association with liver tumors in the Great Lakes region
Endocrine Disrupting Chemicals and Prevalence of Intersex in Fish Populations in New Jersey
NJ WSC and Leetown Science Center scientists in cooperation with the New Jersey Department of Environmental Protection were tasked with characterizing endocrine disruption in smallmouth bass from New Jersey.
Molecular tools and methodologies
Molecular tools and methodologies are used designed and used for diverse applications. These applications can be used to assess questions of threatened and endangered species, population and conservation biology, ecological communities, microbial diversity, molecular epidemiology and genomics of non-model organisms among many others. Successful integration of these tools and technologies greatly...
New Hepatitis B Virus Discovered in White Sucker (Catostomus commersonii)
The first known hepatitis B virus to infect fish (white sucker; Catostomus commersonii) has been discovered by U.S. Geological Survey (USGS) scientists.
Channel Catfish (Ictalurus punctatus) White Blood Cells are Functionally Modulated by Estrogens
Estrogens are recognized as modulators of immune responses in mammals, but how these effects might occur in fish was not understood. U.S. Geological Survey (USGS) scientists published findings that fish white blood cells (leukocytes) have specific estrogen receptors (ERs)—a discovery that moves scientists one step closer to understanding the connection between exposure to estrogenic substances and...
Biological Activity of Steroid Hormones in U.S. Streams
Testing of U.S. streams has detected glucocorticoid and androgen biological activity. In a collaborative study between the National Cancer Institute (NCI), Laboratory of Receptor Biology and Gene Expression, the U.S. Geological Survey (USGS), and others, scientists studied the potential for the biological activity in streams of glucocorticoids and androgens hormones—both potential endocrine...
Filter Total Items: 27
Water quality and contaminants in stream surface waters collected in the Shenandoah Valley, 2021 Water quality and contaminants in stream surface waters collected in the Shenandoah Valley, 2021
The data presented in this data release includes 11 field/water quality parameters, concentrations of 16 nutrients/anions, dissolved organic carbon, 14 organic contaminants, net estrogenicity concentrations, and 51 inorganic constituents in surface water collected twice from 28 stream sites and collected once from 2 stream sites in the Shenandoah Valley (Virginia and West Virginia, USA)...
Results from U.S. Geological Survey Environmental Health Food Resources Lifecycle Integrated Science Team, Graton Pesticides (GRAPE) Study, in Graton, California, April–May 2021 Results from U.S. Geological Survey Environmental Health Food Resources Lifecycle Integrated Science Team, Graton Pesticides (GRAPE) Study, in Graton, California, April–May 2021
In the spring of 2021, six households with private wells were selected throughout Graton, California, based on proximity to agriculture (within one mile of agriculture), well depth (i.e., less than 150 feet in depth), and well type (i.e., dug versus drilled). Silicone bands were deployed for 30 days at outdoor locations at each household to passively sample pesticides in ambient air. On...
Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions.
Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Also included are water quality and chemical concentration data.
Organic and inorganic constituents in surface water and native and non-native fish and frog health data collected from streams, impoundments and wetlands in the New Jersey Pinelands, 2017-2020 (ver. 1.1, August 2022) Organic and inorganic constituents in surface water and native and non-native fish and frog health data collected from streams, impoundments and wetlands in the New Jersey Pinelands, 2017-2020 (ver. 1.1, August 2022)
The data presented include concentrations of 122 organic, 23 inorganic constituents, and estrogenicity in water collected from 13 off-stream non-point source sites (natural and created wetlands) and eight on-stream point source sites (streams and impoundments) in the New Jersey Pinelands, 2017-2020. Native and non-native fish health data collected from the on-stream sites as well as...
Water-quality results from a wastewater reuse study: Inorganic and organic compositions of wastewater effluent and select urban and agricultural water types during rain-induced runoff, Chickasha, Oklahoma, 2018-2019 Water-quality results from a wastewater reuse study: Inorganic and organic compositions of wastewater effluent and select urban and agricultural water types during rain-induced runoff, Chickasha, Oklahoma, 2018-2019
Oklahoma State University South-Central Research Station (SCRS) was used to conduct research to understand the chemical composition of various water types and their potential environmental and human health effects. The study area provided the opportunity to study five water types: (1) receiving surface water (Washita River), (2) urban stormwater, (3) wastewater treatment plant effluent...
SARS-CoV-2 Data from National Wastewater Surveillance System Surge Capacity Sampling, September 2021 SARS-CoV-2 Data from National Wastewater Surveillance System Surge Capacity Sampling, September 2021
Wastewater-based epidemiology (WBE) is used to determine the consumption of, or exposure to, chemicals or pathogens in human populations, and is conducted by collecting representative samples of untreated wastewater (influent) to quantify pathogens shed in the population served by the sampled wastewater system. The Centers for Disease Control and Prevention (CDC) and the US Department of...
Filter Total Items: 8
No results found.
Filter Total Items: 109
Immunomodulation in adult largemouth bass (Micropterus salmoides) exposed to a model estrogen or mixture of endocrine disrupting contaminants during early gonadal recrudescence Immunomodulation in adult largemouth bass (Micropterus salmoides) exposed to a model estrogen or mixture of endocrine disrupting contaminants during early gonadal recrudescence
Disease outbreaks, skin lesions, fish kill events, and reproductive abnormalities have been observed in wild populations of Centrarchids in watersheds throughout the United States. Occurrence of synthetic and natural hormones from wastewater treatment plants and livestock operations, pesticides from agricultural land use, and phytoestrogens have been implicated as potential causes of...
Authors
Jessica Leet, Catherine Richter, Rachel Claunch, Robert Gale, Donald Tillitt, Luke R. Iwanowicz
Complete genome sequence of a novel papillomavirus in Siamese fighting fish (Betta splendens) from Trinidad and Tobago Complete genome sequence of a novel papillomavirus in Siamese fighting fish (Betta splendens) from Trinidad and Tobago
Here, we announce the complete genome of a previously undescribed papillomavirus from a betta fish, Betta splendens. The genome is 5,671 bp with a GC content of 38.2%. Variants were detected in public databases. This genome is most similar to papillomaviruses that infect sea bass (52.9 % nucleotide identity).
Authors
Lemar Blake, A. Carla Phillips-Savage, Esteban Soto, Christopher Oura, Arianne Brown-Jordan, Clayton Raines, Christopher Buck, Luke R. Iwanowicz
Contaminant exposure and transport from three potential reuse waters within a single watershed Contaminant exposure and transport from three potential reuse waters within a single watershed
Global demand for safe and sustainable water supplies necessitates a better understanding of contaminant exposures in potential reuse waters. In this study, we compared exposures and load contributions to surface water from the discharge of three reuse waters (wastewater effluent, urban stormwater, and agricultural runoff). Results document substantial and varying organic-chemical...
Authors
Jason Masoner, Dana W. Kolpin, Isabelle Cozzarelli, Paul M. Bradley, Brian Arnall, Kenneth Forshay, James L. Gray, Justin Groves, Michelle Hladik, Laura Hubbard, Luke R. Iwanowicz, Jeanne Jaeschke, Rachael Lane, R. Blaine McCleskey, Bridgette Polite, David Roth, Michael Pettijohn, Michaelah Wilson
By
Water Resources Mission Area, Environmental Health Program, Central Plains Water Science Center, Eastern Ecological Science Center, Colorado Water Science Center, California Water Science Center, Central Midwest Water Science Center, Geology, Energy & Minerals Science Center, Kansas Water Science Center, Oklahoma-Texas Water Science Center, South Atlantic Water Science Center (SAWSC), Upper Midwest Water Science Center, Reston Biogeochemical Processes in Groundwater Laboratory
Modeling risk dynamics of contaminants of emerging concern in a temperate-region wastewater effluent-dominated stream Modeling risk dynamics of contaminants of emerging concern in a temperate-region wastewater effluent-dominated stream
Wastewater effluent-dominated streams are becoming increasingly common worldwide, including in temperate regions, with potential impacts on ecological systems and drinking water sources. We recently quantified the occurrence/spatiotemporal dynamics of pharmaceutical mixtures in a representative temperate-region wastewater effluent-dominated stream (Muddy Creek, Iowa) under baseflow...
Authors
Hui Zhi, Danielle Webb, Jerald Schnoor, Dana W. Kolpin, Rebecca Klaper, Luke Iwanowicz, Gregory LeFevre
Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection
Disease outbreaks, skin lesions, mortality events, and reproductive abnormalities have been observed in wild populations of centrarchids. The presence of estrogenic endocrine disrupting compounds (EEDCs) has been implicated as a potential causal factor for these effects. The effects of prior EEDC exposure on immune response were examined in juvenile largemouth bass (Micropterus salmoides...
Authors
Jessica Leet, Justin Greer, Cathy Richter, Luke R. Iwanowicz, Edward Spinard, Jacquelyn McDonald, Carla Conway, Robert Gale, Donald Tillitt, John Hansen
Transcriptome signatures of wastewater effluent exposure in larval zebrafish vary with seasonal mixture composition in an effluent-dominated stream Transcriptome signatures of wastewater effluent exposure in larval zebrafish vary with seasonal mixture composition in an effluent-dominated stream
Wastewater treatment plant (WWTP) effluent-dominated streams provide critical habitat for aquatic and terrestrial organisms but also continually expose them to complex mixtures of pharmaceuticals that can potentially impair growth, behavior, and reproduction. Currently, few biomarkers are available that relate to pharmaceutical-specific mechanisms of action. In the experiment reported in...
Authors
Emma Meade, Luke R. Iwanowicz, Nicklaus Neureuther, Gregory LeFevre, Dana W. Kolpin, Hui Zhi, Shannon Meppelink, Rachael Lane, Angela Schmoldt, Aurash Mohaimani, Olaf Mueller, Rebecca Klaper
Non-USGS Publications**
Iwanowicz, L., L Brown, B Eltz, F Juanes and J Murt (2004) Book Review. In a Perfect Ocean: The state of fisheries and ecosystems in the North Atlantic Ocean. Reviews in Fish Biology and Fisheries.14: 149-151.
McIntire, M., L R Iwanowicz and A E Goodwin (2003) Molecular, physical and clinical evidence that Golden Shiner Virus (GSV) and Grass Carp Reovirus (GCRV) are variants of the same virus. Journal of Aquatic Animal Health. 15: 257-263.
Iwanowicz, L.R. and A E Goodwin (2002) Characterization of a new bacilliform fathead minnow rhabdovirus that produces syncytia in tissue culture. Archives of Virology 147: 889 – 916.
Iwanowicz, L.R. A E Goodwin and J Harshbarger (2001) Embryonal rhabdomyosarcoma of the giant gourami, Colisa fasciata (Bloch & Schneider). Journal of Fish Diseases 24:177-179
Iwanowicz, L.R. A E Goodwin and N Heil (2000) A small RNA virus isolated from apparently healthy wild sandbar shiners, Notropis scepticus. Journal of Fish Diseases 23: 349-352.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Filter Total Items: 3
Science and Products
Filter Total Items: 12
Prevalence of novel, emerging hepatitis viruses in wild white suckers and their association with liver tumors in the Great Lakes region
Prevalence of novel, emerging hepatitis viruses in wild white suckers and their association with liver tumors in the Great Lakes region
Endocrine Disrupting Chemicals and Prevalence of Intersex in Fish Populations in New Jersey
NJ WSC and Leetown Science Center scientists in cooperation with the New Jersey Department of Environmental Protection were tasked with characterizing endocrine disruption in smallmouth bass from New Jersey.
Molecular tools and methodologies
Molecular tools and methodologies are used designed and used for diverse applications. These applications can be used to assess questions of threatened and endangered species, population and conservation biology, ecological communities, microbial diversity, molecular epidemiology and genomics of non-model organisms among many others. Successful integration of these tools and technologies greatly...
New Hepatitis B Virus Discovered in White Sucker (Catostomus commersonii)
The first known hepatitis B virus to infect fish (white sucker; Catostomus commersonii) has been discovered by U.S. Geological Survey (USGS) scientists.
Channel Catfish (Ictalurus punctatus) White Blood Cells are Functionally Modulated by Estrogens
Estrogens are recognized as modulators of immune responses in mammals, but how these effects might occur in fish was not understood. U.S. Geological Survey (USGS) scientists published findings that fish white blood cells (leukocytes) have specific estrogen receptors (ERs)—a discovery that moves scientists one step closer to understanding the connection between exposure to estrogenic substances and...
Biological Activity of Steroid Hormones in U.S. Streams
Testing of U.S. streams has detected glucocorticoid and androgen biological activity. In a collaborative study between the National Cancer Institute (NCI), Laboratory of Receptor Biology and Gene Expression, the U.S. Geological Survey (USGS), and others, scientists studied the potential for the biological activity in streams of glucocorticoids and androgens hormones—both potential endocrine...
Filter Total Items: 27
Water quality and contaminants in stream surface waters collected in the Shenandoah Valley, 2021 Water quality and contaminants in stream surface waters collected in the Shenandoah Valley, 2021
The data presented in this data release includes 11 field/water quality parameters, concentrations of 16 nutrients/anions, dissolved organic carbon, 14 organic contaminants, net estrogenicity concentrations, and 51 inorganic constituents in surface water collected twice from 28 stream sites and collected once from 2 stream sites in the Shenandoah Valley (Virginia and West Virginia, USA)...
Results from U.S. Geological Survey Environmental Health Food Resources Lifecycle Integrated Science Team, Graton Pesticides (GRAPE) Study, in Graton, California, April–May 2021 Results from U.S. Geological Survey Environmental Health Food Resources Lifecycle Integrated Science Team, Graton Pesticides (GRAPE) Study, in Graton, California, April–May 2021
In the spring of 2021, six households with private wells were selected throughout Graton, California, based on proximity to agriculture (within one mile of agriculture), well depth (i.e., less than 150 feet in depth), and well type (i.e., dug versus drilled). Silicone bands were deployed for 30 days at outdoor locations at each household to passively sample pesticides in ambient air. On...
Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions.
Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Also included are water quality and chemical concentration data.
Organic and inorganic constituents in surface water and native and non-native fish and frog health data collected from streams, impoundments and wetlands in the New Jersey Pinelands, 2017-2020 (ver. 1.1, August 2022) Organic and inorganic constituents in surface water and native and non-native fish and frog health data collected from streams, impoundments and wetlands in the New Jersey Pinelands, 2017-2020 (ver. 1.1, August 2022)
The data presented include concentrations of 122 organic, 23 inorganic constituents, and estrogenicity in water collected from 13 off-stream non-point source sites (natural and created wetlands) and eight on-stream point source sites (streams and impoundments) in the New Jersey Pinelands, 2017-2020. Native and non-native fish health data collected from the on-stream sites as well as...
Water-quality results from a wastewater reuse study: Inorganic and organic compositions of wastewater effluent and select urban and agricultural water types during rain-induced runoff, Chickasha, Oklahoma, 2018-2019 Water-quality results from a wastewater reuse study: Inorganic and organic compositions of wastewater effluent and select urban and agricultural water types during rain-induced runoff, Chickasha, Oklahoma, 2018-2019
Oklahoma State University South-Central Research Station (SCRS) was used to conduct research to understand the chemical composition of various water types and their potential environmental and human health effects. The study area provided the opportunity to study five water types: (1) receiving surface water (Washita River), (2) urban stormwater, (3) wastewater treatment plant effluent...
SARS-CoV-2 Data from National Wastewater Surveillance System Surge Capacity Sampling, September 2021 SARS-CoV-2 Data from National Wastewater Surveillance System Surge Capacity Sampling, September 2021
Wastewater-based epidemiology (WBE) is used to determine the consumption of, or exposure to, chemicals or pathogens in human populations, and is conducted by collecting representative samples of untreated wastewater (influent) to quantify pathogens shed in the population served by the sampled wastewater system. The Centers for Disease Control and Prevention (CDC) and the US Department of...
Filter Total Items: 8
No results found.
Filter Total Items: 109
Immunomodulation in adult largemouth bass (Micropterus salmoides) exposed to a model estrogen or mixture of endocrine disrupting contaminants during early gonadal recrudescence Immunomodulation in adult largemouth bass (Micropterus salmoides) exposed to a model estrogen or mixture of endocrine disrupting contaminants during early gonadal recrudescence
Disease outbreaks, skin lesions, fish kill events, and reproductive abnormalities have been observed in wild populations of Centrarchids in watersheds throughout the United States. Occurrence of synthetic and natural hormones from wastewater treatment plants and livestock operations, pesticides from agricultural land use, and phytoestrogens have been implicated as potential causes of...
Authors
Jessica Leet, Catherine Richter, Rachel Claunch, Robert Gale, Donald Tillitt, Luke R. Iwanowicz
Complete genome sequence of a novel papillomavirus in Siamese fighting fish (Betta splendens) from Trinidad and Tobago Complete genome sequence of a novel papillomavirus in Siamese fighting fish (Betta splendens) from Trinidad and Tobago
Here, we announce the complete genome of a previously undescribed papillomavirus from a betta fish, Betta splendens. The genome is 5,671 bp with a GC content of 38.2%. Variants were detected in public databases. This genome is most similar to papillomaviruses that infect sea bass (52.9 % nucleotide identity).
Authors
Lemar Blake, A. Carla Phillips-Savage, Esteban Soto, Christopher Oura, Arianne Brown-Jordan, Clayton Raines, Christopher Buck, Luke R. Iwanowicz
Contaminant exposure and transport from three potential reuse waters within a single watershed Contaminant exposure and transport from three potential reuse waters within a single watershed
Global demand for safe and sustainable water supplies necessitates a better understanding of contaminant exposures in potential reuse waters. In this study, we compared exposures and load contributions to surface water from the discharge of three reuse waters (wastewater effluent, urban stormwater, and agricultural runoff). Results document substantial and varying organic-chemical...
Authors
Jason Masoner, Dana W. Kolpin, Isabelle Cozzarelli, Paul M. Bradley, Brian Arnall, Kenneth Forshay, James L. Gray, Justin Groves, Michelle Hladik, Laura Hubbard, Luke R. Iwanowicz, Jeanne Jaeschke, Rachael Lane, R. Blaine McCleskey, Bridgette Polite, David Roth, Michael Pettijohn, Michaelah Wilson
By
Water Resources Mission Area, Environmental Health Program, Central Plains Water Science Center, Eastern Ecological Science Center, Colorado Water Science Center, California Water Science Center, Central Midwest Water Science Center, Geology, Energy & Minerals Science Center, Kansas Water Science Center, Oklahoma-Texas Water Science Center, South Atlantic Water Science Center (SAWSC), Upper Midwest Water Science Center, Reston Biogeochemical Processes in Groundwater Laboratory
Modeling risk dynamics of contaminants of emerging concern in a temperate-region wastewater effluent-dominated stream Modeling risk dynamics of contaminants of emerging concern in a temperate-region wastewater effluent-dominated stream
Wastewater effluent-dominated streams are becoming increasingly common worldwide, including in temperate regions, with potential impacts on ecological systems and drinking water sources. We recently quantified the occurrence/spatiotemporal dynamics of pharmaceutical mixtures in a representative temperate-region wastewater effluent-dominated stream (Muddy Creek, Iowa) under baseflow...
Authors
Hui Zhi, Danielle Webb, Jerald Schnoor, Dana W. Kolpin, Rebecca Klaper, Luke Iwanowicz, Gregory LeFevre
Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection Exposure to 17α-ethinylestradiol results in differential susceptibility of largemouth bass (Micropterus salmoides) to bacterial infection
Disease outbreaks, skin lesions, mortality events, and reproductive abnormalities have been observed in wild populations of centrarchids. The presence of estrogenic endocrine disrupting compounds (EEDCs) has been implicated as a potential causal factor for these effects. The effects of prior EEDC exposure on immune response were examined in juvenile largemouth bass (Micropterus salmoides...
Authors
Jessica Leet, Justin Greer, Cathy Richter, Luke R. Iwanowicz, Edward Spinard, Jacquelyn McDonald, Carla Conway, Robert Gale, Donald Tillitt, John Hansen
Transcriptome signatures of wastewater effluent exposure in larval zebrafish vary with seasonal mixture composition in an effluent-dominated stream Transcriptome signatures of wastewater effluent exposure in larval zebrafish vary with seasonal mixture composition in an effluent-dominated stream
Wastewater treatment plant (WWTP) effluent-dominated streams provide critical habitat for aquatic and terrestrial organisms but also continually expose them to complex mixtures of pharmaceuticals that can potentially impair growth, behavior, and reproduction. Currently, few biomarkers are available that relate to pharmaceutical-specific mechanisms of action. In the experiment reported in...
Authors
Emma Meade, Luke R. Iwanowicz, Nicklaus Neureuther, Gregory LeFevre, Dana W. Kolpin, Hui Zhi, Shannon Meppelink, Rachael Lane, Angela Schmoldt, Aurash Mohaimani, Olaf Mueller, Rebecca Klaper
Non-USGS Publications**
Iwanowicz, L., L Brown, B Eltz, F Juanes and J Murt (2004) Book Review. In a Perfect Ocean: The state of fisheries and ecosystems in the North Atlantic Ocean. Reviews in Fish Biology and Fisheries.14: 149-151.
McIntire, M., L R Iwanowicz and A E Goodwin (2003) Molecular, physical and clinical evidence that Golden Shiner Virus (GSV) and Grass Carp Reovirus (GCRV) are variants of the same virus. Journal of Aquatic Animal Health. 15: 257-263.
Iwanowicz, L.R. and A E Goodwin (2002) Characterization of a new bacilliform fathead minnow rhabdovirus that produces syncytia in tissue culture. Archives of Virology 147: 889 – 916.
Iwanowicz, L.R. A E Goodwin and J Harshbarger (2001) Embryonal rhabdomyosarcoma of the giant gourami, Colisa fasciata (Bloch & Schneider). Journal of Fish Diseases 24:177-179
Iwanowicz, L.R. A E Goodwin and N Heil (2000) A small RNA virus isolated from apparently healthy wild sandbar shiners, Notropis scepticus. Journal of Fish Diseases 23: 349-352.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Filter Total Items: 3
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government