Jennifer Fike is a Geneticist and Lab Manager at the Fort Collins Science Center.
Jennifer Fike is a Geneticist at the Fort Collins Science Center and lab manager for the Molecular Ecology Lab (MEL). She specializes in all aspects of laboratory benchwork including DNA extraction, PCR, microsatellite development, microsatellite genotyping, Sanger sequencing, qPCR, ddRAD library preparation, shotgun sequencing, SNP genotyping, working with non-invasive genetic samples and eDNA. The data she generates is used in a wide range of genetic and genomic studies including species identification, sex determination, parentage analysis, landscape genetics/genomics and genetic mark-recapture.
Professional Experience
2009 to present, Geneticist, USGS Fort Collins Science Center, Fort Collins, CO
2006-2009, Research Assistant, Wildlife Genetics Laboratory, Purdue University, West Lafayette, IN
2003-2006, Laboratory Technician VII, Wildlife Genetics Laboratory, Purdue University, West Lafayette, IN
Education and Certifications
M.S. in Wildlife/Fisheries Biology, Frostburg State University, 2005
B.S. in Biology, Furman University, 2000
Science and Products
Genetic mark–recapture analysis reveals large annual variation in pre-breeding sex ratio of greater sage-grouse
Assessing arthropod diversity metrics derived from stream environmental DNA: Spatiotemporal variation and paired comparisons with manual sampling
Simulation of genetic change under four removal strategies for a wild horse population
A genetic warning system for a hierarchically structured wildlife monitoring framework
Genetic analyses provide new insight on the mating strategies of the American Black Swift (Cypseloides niger)
New strategies for characterizing genetic structure in wide-ranging, continuously distributed species: a Greater Sage-grouse case study
Population genetics reveals bidirectional fish movement across the Continental Divide via an interbasin water transfer
Historical effective population size of North American hoary bat (Lasiurus cinereus) and challenges to estimating trends in contemporary effective breeding population size from archived samples
Composition and distribution of fish environmental DNA in an Adirondack watershed
Feral horse space use and genetic characteristics from fecal DNA
Characterizing range-wide population divergence in an alpine-endemic bird: A comparison of genetic and genomic approaches
Genetic mark‐recapture analysis of winter faecal pellets allows estimation of population size in Sage Grouse Centrocercus urophasianus
Non-USGS Publications**
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Fort Collins Science Center Labs and Facilities
Molecular Genetics
Molecular Ecology Lab (MEL)
Conservation Genomics
Landscape Genetics
Population Genetics
Molecular Tagging
Environmental DNA (eDNA) Sampling Improves Occurrence and Detection Estimates of Invasive Burmese Pythons and Other Constrictor Snakes in Florida
Investigating Prey of Burmese Pythons using eDNA Methods
Taxonomic Uncertainty
Family Relationships and Mating Systems
Population Models
Whole genome sequencing of three North American large-bodied birds
Reduced representation sequencing and genotyping of Arizona Toads (Anaxyrus microscaphus) from the southwestern United States
Sample collection information and microsatellite data for Northern Black Swifts in 6 colonies across Colorado, New Mexico and Idaho
Voucher and metagenetic sequencing of DNA barcodes of wild-collected bees (Apoidea) from Iowa, USA
Genotypes and cluster definitions for a range-wide greater sage-grouse dataset collected 2005-2017 (ver 1.1, January 2023)
Greater sage-grouse genetic warning system, western United States (ver 1.1, January 2023)
Metabarcode sequencing of aquatic environmental DNA from the Potomac River Watershed, 2015-2020
Microsatellite data, boundaries of subpopulation centers, and estimated effective migration for greater sage-grouse collected in western North America between 1992 and 2015 (ver. 2.0, December 2022)
Genetic variation in hoary bats (Lasiurus cinereus) assessed from archived samples
Taxonomic composition of environmental DNA acquired by filtration from the St. Regis River, New York
Sample collection information, single nucleotide polymorphism, and microsatellite data for white-tailed ptarmigan across the species range generated in the Molecular Ecology Lab during 2016
Metagenetic analysis of stream community composition based on environmental DNA
Science and Products
- Publications
Filter Total Items: 30
Genetic mark–recapture analysis reveals large annual variation in pre-breeding sex ratio of greater sage-grouse
Sex ratio, and the extent to which it varies over time, is an important factor in the demography, management, and conservation of wildlife populations. Greater sage-grouse Centrocercus urophasianus populations in western North America are monitored using counts of males at leks in spring. Population estimates derived from lek-count data typically assume a constant, female-biased sex ratio, yet fewAuthorsJessica E. Shyvers, Brett L Walker, Sara J. Oyler-McCance, Jennifer A. Fike, Barry R. NoonAssessing arthropod diversity metrics derived from stream environmental DNA: Spatiotemporal variation and paired comparisons with manual sampling
BackgroundBenthic invertebrate (BI) surveys have been widely used to characterize freshwater environmental quality but can be challenging to implement at desired spatial scales and frequency. Environmental DNA (eDNA) allows an alternative BI survey approach, one that can potentially be implemented more rapidly and cheaply than traditional methods.MethodsWe evaluated eDNA analogs of BI metrics in tAuthorsAaron Aunins, Sara J. Mueller, Jennifer A. Fike, Robert S. CornmanSimulation of genetic change under four removal strategies for a wild horse population
Small and/or artificially reduced populations can suffer genetic erosion with long-term consequences to population fitness and persistence. However, managers must periodically remove free-roaming horses from the landscape to reduce the risk of habitat degradation. We developed an individual-based population simulation model to evaluate the expected change in genetic diversity after 100 years underAuthorsShawna J Zimmerman, Jennifer A. Fike, Sara J. Oyler-McCanceA genetic warning system for a hierarchically structured wildlife monitoring framework
Genetic variation is a well-known indicator of population fitness yet is not typically included in monitoring programs for sensitive species. Additionally, most programs monitor populations at one scale, which can lead to potential mismatches with ecological processes critical to species' conservation. Recently developed methods generating hierarchically nested population units (i.e., clusters ofAuthorsShawna J Zimmerman, Cameron L. Aldridge, Michael O'Donnell, David R. Edmunds, Peter S. Coates, Brian G. Prochazka, Jennifer A. Fike, Todd B. Cross, Bradley C. Fedy, Sara J. Oyler-McCanceGenetic analyses provide new insight on the mating strategies of the American Black Swift (Cypseloides niger)
Avian mating strategies play a vital role in the demographic and genetic dynamics of a species and understanding avian reproductive tactics is important to conservation, population management and restoration. Classifications of avian mating strategies have historically been based on direct physical observations and tend to be rigid population-level generalizations that overlook the variations inheAuthorsCarolyn Gunn, Kim Potter, Jennifer A. Fike, Sara J. Oyler-McCanceNew strategies for characterizing genetic structure in wide-ranging, continuously distributed species: a Greater Sage-grouse case study
Characterizing genetic structure across a species’ range is relevant for management and conservation as it can be used to define population boundaries and quantify connectivity. Wide-ranging species residing in continuously distributed habitat pose substantial challenges for the characterization of genetic structure as many analytical methods used are less effective when isolation by distance is aAuthorsSara J. Oyler-McCance, Todd B. Cross, Jeffery R. Row, Michael K. Schwartz, Dave E. Naugle, Jennifer A. Fike, Kristopher J. Winiarski, Brad C. FedyPopulation genetics reveals bidirectional fish movement across the Continental Divide via an interbasin water transfer
Interbasin water transfers are becoming an increasingly common tool to satisfy municipal and agricultural water demand, but their impacts on movement and gene flow of aquatic organisms are poorly understood. The Grand Ditch is an interbasin water transfer that diverts water from tributaries of the upper Colorado River on the west side of the Continental Divide to the upper Cache la Poudre River onAuthorsAudrey Harris, Sara J. Oyler-McCance, Jennifer A. Fike, Matthew P Fairchild, Christopher M Kennedy, Harry J Crockett, Dana L. Winkelman, Yoichiro KannoHistorical effective population size of North American hoary bat (Lasiurus cinereus) and challenges to estimating trends in contemporary effective breeding population size from archived samples
BackgroundHoary bats (Lasiurus cinereus) are among the bat species most commonly killed by wind turbine strikes in the midwestern United States. The impact of this mortality on species census size is not understood, due in part to the difficulty of estimating population size for this highly migratory and elusive species. Genetic effective population size (Ne) could provide an index of changing cenAuthorsRobert S. Cornman, Jennifer A. Fike, Sara J. Oyler-McCance, Paul M. CryanComposition and distribution of fish environmental DNA in an Adirondack watershed
BackgroundEnvironmental DNA (eDNA) surveys are appealing options for monitoring aquatic biodiversity. While factors affecting eDNA persistence, capture and amplification have been heavily studied, watershed-scale surveys of fish communities and our confidence in such need further exploration.MethodsWe characterized fish eDNA compositions using rapid, low-volume filtering with replicate and controlAuthorsRobert S. Cornman, James E. McKenna, Jennifer A. FikeFeral horse space use and genetic characteristics from fecal DNA
Feral horses (Equus ferus caballus) in the western United States are managed by the Bureau of Land Management (BLM) and United States Forest Service in designated areas on public lands with a goal of maintaining populations in balance with multiple uses of the landscape. Small, isolated populations can be at risk of extirpation from stochastic events and deleterious genetic effects resulting fromAuthorsSarah R. B. King, Kathryn A. Schoenecker, Jennifer A. Fike, Sara J. Oyler-McCanceCharacterizing range-wide population divergence in an alpine-endemic bird: A comparison of genetic and genomic approaches
The delineation of intraspecific units that are evolutionarily and demographically distinct is an important step in the development of species-specific management plans. Neutral genetic variation has served as the primary data source for delineating “evolutionarily significant units,” but with recent advances in genomic technology, we now have an unprecedented ability to utilize information aboutAuthorsKathryn Langin, Cameron L. Aldridge, Jennifer A. Fike, Robert S. Cornman, Kathy M Martin, Greg T Wann, Amy E Seglund, Michael A Schroeder, David P Benson, Brad C. Fedy, Jessica R. Young, Scott D. Wilson, Don H Wolfe, Clait E. Braun, Sara J. Oyler-McCanceGenetic mark‐recapture analysis of winter faecal pellets allows estimation of population size in Sage Grouse Centrocercus urophasianus
The Sage Grouse Centrocercus urophasianus is a species of conservation concern throughout its range in western North America. Since the 1950s, the high count of males at leks has been used as an index for monitoring populations. However, the relationship between this lek‐count index and population size is unclear, and its reliability for assessing population trends has been questioned. We used nonAuthorsJessica E Shyvers, Brett L Walker, Sara J. Oyler-McCance, Jennifer A. Fike, Barry R. NoonNon-USGS Publications**
Dharmarajan, G., J.C.Beasley, W.S. Beatty, Z.H.Olson, J. A. Fike and Olin E. Rhodes, Jr. 2016. Genetic co-structuring in host parasite systems: Empirical data from raccoons and raccoon ticks. Ecosphere 7(3):e01269Dharmarajan, G., J.C.Beasley, J. A. Fike and Olin E. Rhodes, Jr. 2014. Effects of landscape, demographic and behavioral factors on kin structure: testing ecological predictions in a mesopredator with high dispersal capability. Animal Conservation. 17(3): 225-234. http://onlinelibrary.wiley.com/doi/10.1111/acv.12086/fullFike, J.A., C.A. Hennessy, M.L. Kennedy, and O.E. Rhodes, Jr. 2013. Eleven microsatellite markers for the eastern gray squirrel (Sciurus carolinensis) and their utility in fox squirrels (Sciurus niger) and red squirrels (Tamiasciurus hudsonicus). Conservation Genetics Resources. 5: 679-681.Dharmarajan, G., J.C.Beasley, J. A. Fike, E. A. Raizman, C. C. Wu, R. M. Pogranichniy and Olin E. Rhodes, Jr. 2012. Effects of kin-structure on disease dynamics in raccoons (Procyon lotor) inhabiting a fragmented landscape. Basic and Applied Ecology. 13:560-567.Atwood, T.C., J.K. Young, J.P. Beckmann, S.W. Breck, J. Fike, O.E. Rhodes Jr, and K.D. Bristow. 2011. Modeling connectivity of black bears in a desert sky island archipelago. Biological Conservation 144: 2851-2862.Kimble, S.J, J.A. Fike, O.E. Rhodes, Jr., and R.N. Williams. 2011. Identification of 12 polymorphic microsatellite loci for the eastern box turtle (Terrapene carolina carolina). Conservation Genetics Resources 3:65-67.Hapeman,P., E.K. Latch, J.A. Fike, O.E. Rhodes, and C.W. Kilpatrick. 2011. Landscape Genetics of Fishers (Martes pennanti) in the Northeast: Dispersal Barriers and Historical Influences. Journal of Heredity 102: 251-259.Unger, S.D., J.A. Fike, T. Sutton, O.E. Rhodes, Jr., and R.N. Williams. 2010. Isolation and development of 12 polymorphic tetranucleotide microsatellite markers for the eastern hellbender (Cryptobranchus alleganiensis alleganiensis) Conservation GeneticsResources 2:89-91.Fike, J.A., T.L. DeVault, and O.E. Rhodes, Jr. 2009. Identification of twenty-four polymorphic microsatellite markers for the double-crested cormorant (Phalacrocorax auritus). Molecular Ecology Resources 9:1183-1185.Fike, J.A., J.C. Beasley, and O.E. Rhodes, Jr. 2009. Isolation of twenty-one polymorphic microsatellite markers for Virginia opossum (Didelphis virginiana). Molecular Ecology Resources 9:1200-1202.Fike, J.A., G. Athrey, R. Bowman, P.L. Leberg, and O.E. Rhodes, Jr. 2009. Development of twenty-five polymorphic microsatellite markers for the endangered red-cockaded woodpecker (Picoides borealis). Conservation Genetics 10:1021-1023.Fike, J.A. and O.E. Rhodes, Jr. 2009. Characterization of twenty-six polymorphic microsatellite markers for the fox squirrel (Sciurus niger) and their utility in other squirrel species. Conservation Genetics 10:1545-1548.Dharmarajan, G., J.A. Fike, J.C. Beasley and O.E. Rhodes, Jr. 2009. Development and characterization of 12 polymorphic microsatellite loci in the American dog tick (Dermacentor variabilis). Molecular Ecology Resources 9:131-133.Dharmarajan, G., J.A. Fike, J.C. Beasley and O.E. Rhodes, Jr. 2009. Development and characterization of 14 polymorphic microsatellite loci in the raccoon tick (Ixodes texanus). Molecular Ecology Resources 9:296-298.Latch, E.K., J.A. Heffelfinger, J.A. Fike, and O.E. Rhodes, Jr. 2009. Species-wide phylogeography of North American mule deer (Odocoileus hemionus): cryptic glacial refugia and postglacial recolonization. Molecular Ecology 18:1730-1745.Dharmarajan, G., J.C. Beasley, J.A. Fike, and O.E. Rhodes, Jr. 2009. Population genetic structure of raccoons (Procyon lotor) in a fragmented landscape. Canadian J. Zoology 87:814-824.Drauch, A. M., B.E. Fisher, E.K. Latch, J. A. Fike and O. E. Rhodes, Jr. 2008. Evaluation of a remnant lake sturgeon population’s utility as a source for reintroductions in the Ohio River system. Conservation Genetics 9:1195-1209.Latch, E.K, D.G. Scognamillo, J.A Fike, M.J. Chamberlain and O.E. Rhodes, Jr. 2008.Landscape genetics of coastal and inland river otters (Lontra canadensis) in Louisiana. Journal of Heredity 99:265-274.Victory, E.R., J.C. Glaubitz, J.A. Fike, O.E. Rhodes, Jr. and K.E. Woeste. 2008. Different histories but similar genetic diversity and structure for black walnut in Indiana and Missouri. Pages 436-445, In Jacobs, D.F. and Michler C.H. (eds) Proceedings, 16th Central Hardwood Forest Conference; 2008 April 8-10: Lafayette, USDA Forest Service Northern Research Station Technical Report NRS-P-24.Fike, J.A., G. Dharmarajan, A. M. Drauch and O.E. Rhodes, Jr. 2007. Development of polymorphic microsatellite loci for raccoons (Procyon lotor). Molecular Ecology Notes 7:525-527.Anderson, S.J., J.A. Fike, G. Dharmarajan, and O.E. Rhodes, Jr. 2007. Characterization of 12 polymorphic microsatellite loci for eastern chipmunks (Tamias striatus). Molecular Ecology Notes 7:513-515.Beheler, A.S., J.A. Fike and O.E. Rhodes, Jr. 2007. Eight new polymorphic microsatellite loci from the eastern phoebe (Sayornis phoebe). Conservation Genetics 8:1259-1261.Beheler, A. S., J. A. Fike, G. Dharmarajan, O. E. Rhodes, Jr., and T. L. Serfass. 2005. Ten new polymorphic microsatellite loci for North American river otters (Lontracanadensis) and their utility in related mustelids. Molecular Ecology Notes 5:602-604.Beheler, A. S., J. A. Fike, L. M. Murfitt, O. E. Rhodes, Jr., and T. L. Serfass. 2004.Development of polymorphic microsatellite loci for North American river otters (Lontra canadensis) and amplification in related Mustelids. Molecular Ecology Notes 4:56-58.Fike, J.A., T.L. Serfass, A.S. Beheler and O.E. Rhodes, Jr. 2004. Evaluation of preservation methods for DNA analyses of river otter scat: Probability of amplification and genotyping accuracy. Proceedings of the XI International Otter Colloquium Volume 21A.**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
- Science
Fort Collins Science Center Labs and Facilities
The Fort Collins Science Center has scientists working in several diverse locations as well as three in house highly specialized laboratories, studying subjects from molecular ecology to brown treesnakes, dendroecology to streamflows, and macroinvertebrate species to Burmese pythons. Our extraordinary scientists work in each of these locations advancing studies in their particluar areas of study...Molecular Genetics
The use of molecular genetics has become increasingly important in addressing wildlife conservation issues. In the Fort Collins Science Center Molecular Ecology Lab, scientists answer complex questions and conservation issues facing the management of the Nation's fish and wildlife resources. For example, FORT scientists can now locate genes that may contribute to a species' ability to respond to...Molecular Ecology Lab (MEL)
The Molecular Ecology Laboratory applies genetic and genomic technologies to address a variety of complex questions and conservation issues facing the management of the Nation's fish and wildlife resources. Together with our partners, we design and implement studies to document genetic diversity and the distribution of genetic variation among individuals, populations, and species. Information from...Conservation Genomics
Conservation genomics is a new field of science that applies novel whole-genome sequencing technology to problems in conservation biology. Rapidly advancing molecular technologies are revolutionizing wildlife ecology, greatly expanding our understanding of wildlife and their interactions with the environment. In the same way that molecular tools such as microsatellites revolutionized wildlife...Landscape Genetics
Landscape genetics is a recently developed discipline that involves the merger of molecular population genetics and landscape ecology. The goal of this new field of study is to provide information about the interaction between landscape features and microevolutionary processes such as gene flow, genetic drift, and selection allowing for the understanding of processes that generate genetic...Population Genetics
Population genetics is an area of research that examines the distribution of genetic variation and levels of genetic diversity within and between populations. This information provides insights into the level of connectedness of populations throughout a species’ range and can be used to identify unique populations or those with low levels of genetic diversity.Molecular Tagging
Molecular tagging is a new application of molecular genetic techniques to traditional mark-recapture methodology designed to address situations where traditional methods fail. In such studies, non-invasively collected samples (such as feces, feathers, or fur) are used as a source of DNA that is then genotyped at multiple loci such that each individual animal can be uniquely identified. Thus, each...Environmental DNA (eDNA) Sampling Improves Occurrence and Detection Estimates of Invasive Burmese Pythons and Other Constrictor Snakes in Florida
Environmental DNA (eDNA) is organismal DNA that can be found in the environment. Environmental DNA originates from cellular material shed by organisms (via skin, excrement, etc.) into aquatic or terrestrial environments that can be sampled and monitored using new molecular methods. Such methodology is important for the early detection of invasive species as well as the detection of rare and...Investigating Prey of Burmese Pythons using eDNA Methods
Environmental DNA (eDNA) is organismal DNA that can be found in the environment. Environmental DNA originates from cellular material shed by organisms (via skin, excrement, etc.) into aquatic or terrestrial environments that can be sampled and monitored using new molecular methods. Such methodology is important for the early detection of invasive species as well as the detection of rare and...Taxonomic Uncertainty
Taxonomic uncertainty can be assessed using genetic data, along with other lines of evidence (such as morphological and behavioral characteristics). Such data can be used to identify and assess taxonomic boundaries (species, subspecies, hybrids) and in many cases redefine them. Such delineations are highly relevant for species status determinations (endangered, threatened, or at-risk).Family Relationships and Mating Systems
Family relationships and mating systems can be investigated and defined using genetic data. This information is potentially important for conservation and management as it may influence effective population size and levels of genetic diversity.Population Models
Population models can incorporate genetic data to assess potential impacts of different management strategies on connectivity, effective population size, and genetic diversity. - Data
Filter Total Items: 13
Whole genome sequencing of three North American large-bodied birds
The data release details the samples, methods, and raw data used to generate high-quality genome assemblies for greater sage-grouse (Centrocercus urophasianus), white-tailed ptarmigan (Lagopus leucura), and trumpeter swan (Cygnus buccinator). The raw data have been deposited in the Sequence Read Archive (SRA) of the National Center for Biotechnology Information (NCBI), the authoritative repositoryReduced representation sequencing and genotyping of Arizona Toads (Anaxyrus microscaphus) from the southwestern United States
The dataset consists of genotypes (diploid base calls at variant sites) at 3,601 anonymous sites of the Arizona Toad (Anaxyrus microscaphus) nuclear genome. The genotyped samples are representative of the range of the species and its major population units, and the genotyped loci have a high degree of completeness. This data release consists of several files: 1. sample.metadata.txt, which containSample collection information and microsatellite data for Northern Black Swifts in 6 colonies across Colorado, New Mexico and Idaho
This dataset contains sample collection information and microsatellite genotype data for Northern Black Swifts in 6 colonies across Colorado, New Mexico and Idaho. This data was collected to examine levels of genetic diversity and connectivity among Black Swift colonies and also to assess parentage at a subset of those sites. This study helps clarify the breeding system of the Black Swift and is tVoucher and metagenetic sequencing of DNA barcodes of wild-collected bees (Apoidea) from Iowa, USA
High-throughput methods for identification of pollinator taxa are desirable to improve our understanding of pollinator distributions, population trends, and ecology. Genetic sequencing of taxonomically informative 'barcode' loci is one high-throughput strategy, which can be applied to individual specimens using Sanger technology and to complex mixtures using metabarcoding technology. This study geGenotypes and cluster definitions for a range-wide greater sage-grouse dataset collected 2005-2017 (ver 1.1, January 2023)
Monitoring change in genetic diversity in wildlife populations across multiple scales could facilitate prioritization of conservation efforts. We used microsatellite genotypes from 7,080 previously collected genetic samples from across the greater sage-grouse (Centrocercus urophasianus) range to develop a modelling framework for estimating genetic diversity within a recently developed hierarchicalGreater sage-grouse genetic warning system, western United States (ver 1.1, January 2023)
Genetic variation is a well-known indicator of population fitness yet is not typically included in monitoring programs for sensitive species. Additionally, most programs monitor populations at one scale, which can lead to potential mismatches with ecological processes critical to species’ conservation. Recently developed methods generating hierarchically nested population units (i.e., clusters ofMetabarcode sequencing of aquatic environmental DNA from the Potomac River Watershed, 2015-2020
Biological indicator taxa have long been used for integrative assessments of water quality, particularly benthic invertebrate groups such as arthropods. While standardized protocols have been developed to calculate 'biological index' scores based on the abundances of these taxa, such systems are challenging to implement at large scales due to the sampling effort required, taxonomic expertise needeMicrosatellite data, boundaries of subpopulation centers, and estimated effective migration for greater sage-grouse collected in western North America between 1992 and 2015 (ver. 2.0, December 2022)
Greater Sage-grouse were sampled for genetic analysis with the goal of quantifying genetic structure and gene flow across the entire species range in the U.S. and Canada. Data presented here consist of two data sets both including genetic data from 15 microsatellite markers. Most samples were collected between 2005 and 2015 (feathers collected non-invasively off the ground) yet some samples were bGenetic variation in hoary bats (Lasiurus cinereus) assessed from archived samples
Hoary bats are among the bat species most commonly killed by turbine strikes in the midwestern United States. The impact of this mortality on species census size is not understood, due in part to the difficulty of estimating population size for this highly migratory and elusive species. Genetic effective population size (Ne) could provide an index of census population size if other factors affectiTaxonomic composition of environmental DNA acquired by filtration from the St. Regis River, New York
Environmental DNA (eDNA) surveys have become important tools for monitoring aquatic biodiversity. Barcode sequencing of eDNA generates community profiles that, while potentially biased in both capture and amplification, can nonetheless yield high information content per unit cost. While factors affecting eDNA capture and amplification have been heavily studied, watershed-scale assessments of fishSample collection information, single nucleotide polymorphism, and microsatellite data for white-tailed ptarmigan across the species range generated in the Molecular Ecology Lab during 2016
This data release comprises a dataset that contains sample collection information and microsatellite genotypes, and another dataset that contains single nucleotide polymorphism (SNP) genotypes with sample collection information for populations of white-tailed ptarmigan across the species' range. There is also an additional file (accession numbers.xlsx) linking samples to accession numbers in GenbaMetagenetic analysis of stream community composition based on environmental DNA
A survey of environmental DNA was performed in Tunison Creek downstream of the USGS Tunison Aquatic Laboratory. The goal of the survey was to characterize the source ecological community at multiple trophic or taxonomic levels by associating DNA fragments with reference databases. Three taxonomically informative genetic loci were used: the mitochondrial cytochrome oxidase 1 locus, the mitochondria - Multimedia