A Japanese quail egg is being examined to determine if the egg is viable. Associated project: Development and optimization of toxicogenomic tools for assessing immunomodulatory, metabolic, and endocrine effects of emerging contaminants.
Natalie Karouna-Renier, Ph.D.
Natalie is a Research Ecologist and leader of the Molecular Toxicology Laboratory at Eastern Ecological Science Center in Laurel, MD. Natalie's expertise is in molecular toxicology with a focus on understanding, detecting, and predicting the effects of environmental stressors on wildlife.
Natalie's laboratory uses genomic, transcriptomic, metabolomic, microbiomic, endocrine, and biochemical tools to develop novel biomarkers and assessment tools and applies these in collaborative field and laboratory studies that investigate regional, national, and international environmental health questions. Current projects in her lab fall under the broad scope of ecotoxicology, focusing on immune system and disease susceptibility, endocrine disruption, genotoxicity, and health status of birds and other wildlife. Hazards and toxicity of contemporary contaminants, such as perfluorinated compounds (PFAS), flame retardants and neonicotinoid pesticides are of particular interest. The data are used for forecasting effects on individuals and populations, identifying wildlife that are potentially being harmed, assessing damage to trust resources, and otherwise informing natural resource management decisions.
Science and Products
Filter Total Items: 19
Wild Prairie Grouse Diet and Microbiomes Vary Between Cropland and Grassland Habitats
Wild prairie grouse residing in croplands had altered diets and gut microbiome imbalances characterized by a greater abundance of pathogenic bacteria and antibiotic-resistance genes in comparison to those residing in grasslands. Similar gut microbiome imbalances are rarely associated with lethal outcomes, but rather linked to sublethal health effects including growth, development, behavior, immune...
PFAS Transport, Exposure, and Effects
The team is determining the movement and behavior of per- and poly-fluoroalkyl substances (PFAS) from their sources in the environment, as they move through exposure pathways in ecosystems including watersheds and aquifers, their incorporation into food webs, and molecular to population scale effects on fish and wildlife. These studies are accomplished at a variety of spatial scales from regional...
Immunomodulation Science Team
The Immunomodulation Integrated Science Team focuses on contaminant and pathogen exposures in the environment that might influence the immune systems of wildlife and the connection to their shared environment with humans. In collaboration with public-health officials, the Team also addresses potential human-health risks stemming from similar exposures. If actual risks are identified, this Team...
Bird Hatchling Development Following Exposure to Environmentally Relevant Levels of Brominated Flame Retardants
Scientists performed laboratory studies with American kestrels and zebra finch to determine the effects of brominated flame retardant exposure on hatchlings. They found evidence that exposure while in the egg disrupted thyroid function in female American kestrel hatchlings and affected nestling body condition in zebra finch hatchlings, but no other effects were detected.
Region 1: North Atlantic-Appalachian PFAS Capability Team
Per- and polyfluoroalkyl substances (PFAS) are a group of anthropogenic emerging contaminants. Some PFAS have been voluntarily phased out, as exposure has been linked to adverse human health effects.
Immune System Changes and Susceptibility to Disease in Birds Exposed to Environmental Contaminants
Disease dynamics in wildlife are commonly related to changes or increases in environmental stressors that are placed upon an animal. Environmental pollutants are known to affect the immune system of wildlife, resulting in impaired resistance to infection and potential increases in disease outbreaks. In collaboration with: Jill Jenkins, Ph.D USGS Wetland and Aquatic Research Center; Julia Lankton...
Effects of Short Chain Chlorinated Paraffins (SCCPs) on developing birds
The Challenge: Short-Chain Chlorinated Paraffins (SCCPs) are complex technical mixtures of polychlorinated n-alkanes used in lubricants and coolants in metalworking, as flame retardants, and in paints, adhesives, sealants, textiles and polymeric materials, plastics and rubber. SCCPs are of concern because they are globally transported, bioaccumulate in wildlife and humans, and are environmentally...
Do pesticide coatings on agricultural seeds pose a threat to earthworms and to the birds that eat them?
Earthworms are a vital part of a healthy, functioning soil ecosystem and are also an important protein-rich food source for wildlife, including many species of birds and mammals. In an agricultural setting, the presence of earthworms can increase crop yields by 25%, but can also expose earthworms to pesticides. Seed treatment is the principal application method for neonicotinoid insecticides, one...
Dysbiosis, immunomodulation, and health effects of agricultural pesticides in wild prairie grouse
Agrochemical pollution poses a severe threat to biodiversity. Agrochemicals can detrimentally affect wildlife growth, development, survival, reproduction, and immune responses, which facilitates emergence and spread of infectious diseases that may cause unusually high mortality. Animal microbiota plays a fundamental role in host’s food detoxification and defense against pathogens, regulates...
Contaminant Exposure, Food Web Transfer and Potential Health Effects on Chesapeake Bay and Delaware Bay Waterbirds
Chesapeake and Delaware Bays are the largest estuaries on the east coast of the United States. They support extensive fisheries and provide critical habitat for many species of wildlife. These Bays are surrounded by vast agricultural andindustrialized regions, with urbanized areas, all of which are a source of pollution that can affect natural resources. We have long monitored contaminants in this...
Studying Immune Responses in the American Kestrel (Falco sparverius)
The objectives of this study are to (1) investigate whether the immune system will respond to chemical stressors, such as new flame retardant compounds, and (2) determine if such chemical stressors influence white blood cells (WBC) responses after exposure to a viral pathogen mimicking bird flu.
Genomic and Behavioral Effects of the Neonicotinoid Imidacloprid in Birds Exposed Through Pesticide-Coated Seeds
The Challenge: Neonicotinoid pesticides act as agonists of nicotinic acetylcholine receptors (nAChRs) and are designed to be lethal to insects while theoretically posing little to no threat to vertebrates. The perceived safety of neonicotinoids has led to a sharp increase in their use in the United States and globally, since they were first introduced in 1994. The use of the neonicotinoid...
Candling Bird Egg
A Japanese quail egg is being examined to determine if the egg is viable. Associated project: Development and optimization of toxicogenomic tools for assessing immunomodulatory, metabolic, and endocrine effects of emerging contaminants.
Early Life Stage (ELS) Test
Early Life Stage (ELS) tests are used as an alternative to traditional whole-animal testing methods for evaluating toxicity of chemicals. ELS for birds involve injection into the air cell of fertile eggs early in development and artificial incubation for several days at which point tissues are sampled for analysis.
Early Life Stage (ELS) tests are used as an alternative to traditional whole-animal testing methods for evaluating toxicity of chemicals. ELS for birds involve injection into the air cell of fertile eggs early in development and artificial incubation for several days at which point tissues are sampled for analysis.
American kestrel eggs
American kestrel eggs
American kestrel eggs
American kestrel
American kestrel (Falco sparverius)
American kestrel (Falco sparverius)
American kestrel
American kestrel (Falco sparverius)
American kestrel (Falco sparverius)
USGS Scientist Preparing Samples in a Laboratory
U.S. Geological Survey (USGS) scientist preparing samples for analysis of protein levels to normalize results from oxidative stress bioassays.
U.S. Geological Survey (USGS) scientist preparing samples for analysis of protein levels to normalize results from oxidative stress bioassays.
Caged kestrel egg experiment
American kestrel egg experiment
American kestrel egg experiment
Science and Products
Filter Total Items: 19
Wild Prairie Grouse Diet and Microbiomes Vary Between Cropland and Grassland Habitats
Wild prairie grouse residing in croplands had altered diets and gut microbiome imbalances characterized by a greater abundance of pathogenic bacteria and antibiotic-resistance genes in comparison to those residing in grasslands. Similar gut microbiome imbalances are rarely associated with lethal outcomes, but rather linked to sublethal health effects including growth, development, behavior, immune...
PFAS Transport, Exposure, and Effects
The team is determining the movement and behavior of per- and poly-fluoroalkyl substances (PFAS) from their sources in the environment, as they move through exposure pathways in ecosystems including watersheds and aquifers, their incorporation into food webs, and molecular to population scale effects on fish and wildlife. These studies are accomplished at a variety of spatial scales from regional...
Immunomodulation Science Team
The Immunomodulation Integrated Science Team focuses on contaminant and pathogen exposures in the environment that might influence the immune systems of wildlife and the connection to their shared environment with humans. In collaboration with public-health officials, the Team also addresses potential human-health risks stemming from similar exposures. If actual risks are identified, this Team...
Bird Hatchling Development Following Exposure to Environmentally Relevant Levels of Brominated Flame Retardants
Scientists performed laboratory studies with American kestrels and zebra finch to determine the effects of brominated flame retardant exposure on hatchlings. They found evidence that exposure while in the egg disrupted thyroid function in female American kestrel hatchlings and affected nestling body condition in zebra finch hatchlings, but no other effects were detected.
Region 1: North Atlantic-Appalachian PFAS Capability Team
Per- and polyfluoroalkyl substances (PFAS) are a group of anthropogenic emerging contaminants. Some PFAS have been voluntarily phased out, as exposure has been linked to adverse human health effects.
Immune System Changes and Susceptibility to Disease in Birds Exposed to Environmental Contaminants
Disease dynamics in wildlife are commonly related to changes or increases in environmental stressors that are placed upon an animal. Environmental pollutants are known to affect the immune system of wildlife, resulting in impaired resistance to infection and potential increases in disease outbreaks. In collaboration with: Jill Jenkins, Ph.D USGS Wetland and Aquatic Research Center; Julia Lankton...
Effects of Short Chain Chlorinated Paraffins (SCCPs) on developing birds
The Challenge: Short-Chain Chlorinated Paraffins (SCCPs) are complex technical mixtures of polychlorinated n-alkanes used in lubricants and coolants in metalworking, as flame retardants, and in paints, adhesives, sealants, textiles and polymeric materials, plastics and rubber. SCCPs are of concern because they are globally transported, bioaccumulate in wildlife and humans, and are environmentally...
Do pesticide coatings on agricultural seeds pose a threat to earthworms and to the birds that eat them?
Earthworms are a vital part of a healthy, functioning soil ecosystem and are also an important protein-rich food source for wildlife, including many species of birds and mammals. In an agricultural setting, the presence of earthworms can increase crop yields by 25%, but can also expose earthworms to pesticides. Seed treatment is the principal application method for neonicotinoid insecticides, one...
Dysbiosis, immunomodulation, and health effects of agricultural pesticides in wild prairie grouse
Agrochemical pollution poses a severe threat to biodiversity. Agrochemicals can detrimentally affect wildlife growth, development, survival, reproduction, and immune responses, which facilitates emergence and spread of infectious diseases that may cause unusually high mortality. Animal microbiota plays a fundamental role in host’s food detoxification and defense against pathogens, regulates...
Contaminant Exposure, Food Web Transfer and Potential Health Effects on Chesapeake Bay and Delaware Bay Waterbirds
Chesapeake and Delaware Bays are the largest estuaries on the east coast of the United States. They support extensive fisheries and provide critical habitat for many species of wildlife. These Bays are surrounded by vast agricultural andindustrialized regions, with urbanized areas, all of which are a source of pollution that can affect natural resources. We have long monitored contaminants in this...
Studying Immune Responses in the American Kestrel (Falco sparverius)
The objectives of this study are to (1) investigate whether the immune system will respond to chemical stressors, such as new flame retardant compounds, and (2) determine if such chemical stressors influence white blood cells (WBC) responses after exposure to a viral pathogen mimicking bird flu.
Genomic and Behavioral Effects of the Neonicotinoid Imidacloprid in Birds Exposed Through Pesticide-Coated Seeds
The Challenge: Neonicotinoid pesticides act as agonists of nicotinic acetylcholine receptors (nAChRs) and are designed to be lethal to insects while theoretically posing little to no threat to vertebrates. The perceived safety of neonicotinoids has led to a sharp increase in their use in the United States and globally, since they were first introduced in 1994. The use of the neonicotinoid...
Candling Bird Egg
A Japanese quail egg is being examined to determine if the egg is viable. Associated project: Development and optimization of toxicogenomic tools for assessing immunomodulatory, metabolic, and endocrine effects of emerging contaminants.
A Japanese quail egg is being examined to determine if the egg is viable. Associated project: Development and optimization of toxicogenomic tools for assessing immunomodulatory, metabolic, and endocrine effects of emerging contaminants.
Early Life Stage (ELS) Test
Early Life Stage (ELS) tests are used as an alternative to traditional whole-animal testing methods for evaluating toxicity of chemicals. ELS for birds involve injection into the air cell of fertile eggs early in development and artificial incubation for several days at which point tissues are sampled for analysis.
Early Life Stage (ELS) tests are used as an alternative to traditional whole-animal testing methods for evaluating toxicity of chemicals. ELS for birds involve injection into the air cell of fertile eggs early in development and artificial incubation for several days at which point tissues are sampled for analysis.
American kestrel eggs
American kestrel eggs
American kestrel eggs
American kestrel
American kestrel (Falco sparverius)
American kestrel (Falco sparverius)
American kestrel
American kestrel (Falco sparverius)
American kestrel (Falco sparverius)
USGS Scientist Preparing Samples in a Laboratory
U.S. Geological Survey (USGS) scientist preparing samples for analysis of protein levels to normalize results from oxidative stress bioassays.
U.S. Geological Survey (USGS) scientist preparing samples for analysis of protein levels to normalize results from oxidative stress bioassays.
Caged kestrel egg experiment
American kestrel egg experiment
American kestrel egg experiment