Rickard, A.H., R.J. Palmer, Jr., D.S. Blehert, S.R. Campagna, M.F. Semmelhack, P.G. Egland, B.L. Bassler, and P.E. Kolenbrander. 2006. Autoinducer 2: a concentration-dependent signal for mutualistic bacterial biofilm growth. Molecular Microbiology 60: 1446-1456.
David S Blehert
Biography
David Blehert received a Ph.D. in Bacteriology from the University of Wisconsin-Madison in 1999, and he joined the USGS National Wildlife Health Center (NWHC) as a Diagnostic and Research Microbiologist in 2003. His research focuses on investigation of the ecology and pathobiology of bat white-nose syndrome. Today, Dr. Blehert is Chief of the NWHC's Laboratory Sciences Branch, which specializes in the investigation of unusual mortality events impacting our nation's wildlife. His laboratories also conduct surveillance for animal diseases of high consequence, such as white-nose syndrome in bats and highly pathogenic avian influenza viruses in wild waterfowl.
Education
- Ph.D. Bacteriology, University of Wisconsin-Madison, 1999
- B.S. Biology, University of Minnesota-Minneapolis St. Paul, 1993
Professional Experience
- 2014 to present, Chief, Laboratory Sciences Branch, U.S. Geological Survey National Wildlife Health Center, Madison, Wisconsin
- 2003-2014, Diagnostic and Research Microbiologist, U.S. Geological Survey National Wildlife Health Center, Madison, Wisconsin
- 1999 to 2003, Intramural Research Training Associate Fellow, National Institutes of Health, Bethesda, Maryland
Affiliations
- 2003 to present, Honorary Fellow, University of Wisconsin-Madison School of Veterinary Medicine, Department of Pathobiological Sciences
Science and Products
Date published: October 19, 2020
Status: Active Laboratories for Wildlife Disease Diagnostics
Our audience are wildlife disease and natural resource managers who use our labs for wildlife disease diagnostic services. Information they are looking for include information on diagnostic services and tests, contact information, submission forms, sample collection instructions, submission guidelines and procedures.
Attribution: National Wildlife Health Center
Date published: May 7, 2019
Status: Active Transforming Biosurveillance by Standardizing and Serving 40 Years of Wildlife Disease Data
Over the past 40 years the National Wildlife Health center has collected wildlife health information from around the U.S. and beyond, amassing the world’s largest repository of wildlife-disease surveillance data. This project identified, characterized, and documented NWHC’s locally stored wildlife health datasets, a critical first step to migrating them to new laboratory- and public-facing...
Contacts: David S Blehert, C. LeAnn White
Attribution: Community for Data Integration (CDI)
Date published: August 29, 2018
Status: Active Pathology — Madison, Wisconsin
About the Capability
The Environmental Health Program collaborates with the pathology section of the Necropsy and Pathology Diagnostic Laboratory at the National Wildlife Health Center (NWHC) to advance an understanding of the effects of contaminant and pathogen exposure on wildlife.
Contacts: David S Blehert, Julia S Lankton
Date published: July 24, 2018
Status: Active Avian Influenza
Avian influenza is a viral disease caused by various strains of avian influenza viruses that can be classified as low pathogenic avian influenza (LPAI) or highly pathogenic avian influenza (HPAI). It remains a global disease with potential high consequence with the potential to threaten wildlife, agriculture, and human health.
Date published: July 24, 2018
Status: Active Avian Influenza Surveillance
The USGS National Wildlife Health Center (NWHC) serves on the U.S. Interagency Steering Committee for Surveillance for Highly Pathogenic Avian Influenza in Wild Birds to standardize surveillance for this disease and is a leading partner in conducting morbidity and mortality investigations in support of the Interagency Strategic Plan for Early Detection and Monitoring for Avian Influenzas of...
Date published: March 29, 2018
Status: Active Diagnostic Services
The USGS National Wildlife Health Center (NWHC) conducts laboratory investigations to determine the causes of wildlife mortality events, especially large-scale die-offs or those that are otherwise unusual.
Contacts: David S Blehert
Date published: February 1, 2018
Status: Active White-Nose Syndrome
White-nose syndrome (WNS) is an emergent disease of hibernating bats that has spread from the northeastern across United States at an alarming rate.
Date published: September 19, 2017
Status: Completed USGS Scientists Receive Award for Pioneering Work on White-Nose Syndrome in Bats
Dr. Carol U. Meteyer and Dr. David S. Blehert received the Tom Thorne and Beth Williams Memorial Award from the Wildlife Disease Association and the American Association of Wildlife Veterinarians for their pioneering work on white-nose syndrome (WNS) in bats.
Year Published: 2021
Analysis of archival specimens confirms White-nose syndrome in little brown bats (Myotis lucifugus) from New York, USA, in spring 2007
White-nose syndrome (WNS), an emerging fungal disease of North American bats, was first diagnosed in January 2008, although mortality and photo-documentation suggest the disease may have been present earlier. Using archived samples, we describe a definitive case of WNS in little brown bats (Myotis lucifugus) from New York, USA, in spring 2007.
Keller, Saskia; Lorch, Jeffrey M.; Berlowski-Zier, Brenda M.; Ballmann, Anne; Blehert, David S.Attribution: National Wildlife Health Center, Ecosystems
Year Published: 2021
Laboratory maintenance and culture of Pseudogymnoascus destructans, the fungus that causes bat white-nose syndrome
Pseudogymnoascus destructans is a fungal pathogen that causes white‐nose syndrome, an emerging and fatal disease of North American bats that has led to unprecedented population declines. As a psychrophile, P. destructans is adapted to infect bats during winter hibernation, when host metabolic activity and core body temperature are...
Blehert, David S.; Lorch, Jeffrey M.Attribution: National Wildlife Health Center, Ecosystems
Year Published: 2021
Skin fungal assemblages of bats vary based on susceptibility to white-nose syndrome
Microbial skin assemblages, including fungal communities, can influence host resistance to infectious diseases. The diversity-invasibility hypothesis predicts that high-diversity communities are less easily invaded than species-poor communities, and thus diverse microbial communities may prevent pathogens from colonizing a host. To explore the...
Vanderwolf, Karen J; Campbell, Lewis; Goldberg, Tony L; Blehert, David S.; Lorch, Jeffrey M.
Year Published: 2020
Possibility for reverse zoonotic transmission of SARS-CoV-2 to free-ranging wildlife: A case study of bats
The COVID-19 pandemic highlights the substantial public health, economic, and societal consequences of virus spillover from a wildlife reservoir. Widespread human transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) also presents a new set of challenges when considering viral spillover from people to naïve wildlife and...
Olival, Kevin J.; Cryan, Paul M.; Amman, Brian R.; Baric, Ralph S.; Blehert, David S.; Brook, Cara E.; Calisher, Charles H.; Castle, Kevin T.; Coleman, Jeremy TH; Daszak, Peter; Epstein, Jonathan H.; Field, Hume; Frick, Winifred F.; Gilbert, Amy T.; Hayman, David T.S.; Ip, Hon S.; Karesh, William B; Johnson, Christine K.; Kading, Rebekah C; Kingston, Tigga; Lorch, Jeffrey M.; Mendenhall, Ian H; Peel, Alison J.; Phelps, Kendra L; Plowright, Raina K.; Reeder, DeeAnn M; Reichard, Jonathan D.; Sleeman, Jonathan M.; Streicker, Daniel G.; Towner, Jonathan S.; Wang, Lin-Fa
Year Published: 2020
Assessing the risks posed by SARS-CoV-2 in and via North American bats—Decision framing and rapid risk assessment
The novel β-coronavirus, SARS-CoV-2, may pose a threat to North American bat populations if bats are exposed to the virus through interaction with humans, if the virus can subsequently infect bats and be transmitted among them, and if the virus causes morbidity or mortality in bats. Further, if SARS-CoV-2 became established in bat populations, it...
Runge, Michael C.; Campbell Grant, Evan H.; Coleman, Jeremy T. H.; Reichard, Jonathan D.; Gibbs, Samantha E. J.; Cryan, Paul M.; Olival, Kevin J.; Walsh, Daniel P.; Blehert, David S.; Hopkins, M. Camille; Sleeman, Jonathan M.Attribution: National Wildlife Health Center, Patuxent Wildlife Research Center, Eastern Ecological Science Center, Ecosystems, Fish & Wildlife Disease, , Region 1: North Atlantic-Appalachian, United States of America
View Citation
Runge, M.C., Grant, E.H.C., Coleman, J.T.H., Reichard, J.D., Gibbs, S.E.J., Cryan, P.M., Olival, K.J., Walsh, D.P., Blehert, D.S., Hopkins, M.C., and Sleeman, J.M., 2020, Assessing the risks posed by SARS-CoV-2 in and via North American bats—Decision framing and rapid risk assessment: U.S. Geological Survey Open-File Report 2020–1060, 43 p., https://doi.org/10.3133/ofr20201060.
Year Published: 2020
Validation of laboratory tests for infectious diseases in wild mammals: Review and recommendations
Evaluation of the diagnostic sensitivity (DSe) and specificity (DSp) of tests for infectious diseases in wild animals is challenging, and some of the limitations may affect compliance with the OIE-recommended test validation pathway. We conducted a methodologic review of test validation studies for OIE-listed diseases in wild mammals published...
Beibei, Jia; Colling, David; Stallknecht, David E.; Blehert, David S.; Bingham, John; Crossley, Beate; Eagles, Debbie; Gardner, Ian AAttribution: National Wildlife Health Center, Ecosystems
Year Published: 2020
Threats posed by the Fungal Kingdom to humans, wildlife, and agriculture
The Fungal Kingdom includes at least six million eukaryotic species and is remarkable with respect to its profound impact on global health, biodiversity, ecology, agriculture, manufacturing, and biomedical research. Approximately 625 fungal species have been reported to infect vertebrates, 200 of which can be human-associated, either as commensals...
Fisher, Matthew C.; Gurr, Sarah J.; Cuomo, Christina A.; Blehert, David S.; Jin, Hailing; Stukenbrock, Eva H.; Stajich, Jason E.; Kahmann, Regine; Boone, Charles; Denning, David W.; Gow, Neil A. R.; Klein, Bruce S.; Kronstad, James W.; Sheppard, Donald C.; Taylor, John W.; Wright, Gerard D.; Heitman, Joseph; Casadevall, Arturo; Cowen, Leah E.Attribution: National Wildlife Health Center, Ecosystems
Year Published: 2019
Response to “Prepublication communication of research results”: The need for a coordinated wildlife disease surveillance laboratory network
In “Prepublication Communication of Research Results”, Adams et al. (2018) outline the importance of timeliness in providing scientific results with consequential implications for wildlife management to responsible government agencies. The authors discuss various impediments to sharing of scientific results and provide well-reasoned arguments why...
Sleeman, Jonathan M.; Blehert, David S.; Richgels, Katherine L. D.; White, C. LeAnn
Year Published: 2018
Experimental infection of Tadarida brasiliensis with Pseudogymnoascus destructans, the fungus that causes white-nose syndrome
White-nose syndrome (WNS) is causing significant declines in populations of North American hibernating bats, and recent western and southern expansions of the disease have placed additional species at risk. Understanding differences in species susceptibility and identifying management actions to reduce mortality of bats from WNS are top research...
Verant, Michelle; Meteyer, Carol U.; Stading, Benjamin; Blehert, David S.Attribution: Biology and Ecosystems, National Wildlife Health Center, Ecosystems, Fish & Wildlife Disease, United States of America
View Citation
Verant ML, Meteyer CU, Stading B, Blehert DS. 2018. Experimental infection of Tadarida brasiliensis with Pseudogymnoascus destructans, the fungus that causes white-nose syndrome. mSphere 3:e00250-18. https://doi.org/10.1128/mSphere.00250-18
Year Published: 2018
Determinants of Pseudogymnoascus destructans within bat hibernacula: Implications for surveillance and management of white-nose syndrome
Fungal diseases are an emerging global problem affecting human health, food security and biodiversity. Ability of many fungal pathogens to persist within environmental reservoirs can increase extinction risks for host species and presents challenges for disease control. Understanding factors that regulate pathogen spread and persistence in these...
Verant, Michelle L.; Bohuski, Elizabeth A.; Richgels, Katherine L. D.; Olival, Kevin J.; Epstein, Jonathan H.; Blehert, David S.Attribution: Biology and Ecosystems, National Wildlife Health Center, Ecosystems, Fish & Wildlife Disease, United States of America
View Citation
J Appl Ecol. 2018;1–10.
Year Published: 2018
Malassezia vespertilionis sp. nov.: A new cold-tolerant species of yeast isolated from bats
Malassezia is a genus of medically-important, lipid-dependent yeasts that live on the skin of warm-blooded animals. The 17 described species have been documented primarily on humans and domestic animals, but few studies have examined Malassezia species associated with more diverse host groups such as wildlife. While investigating...
Lorch, Jeffrey M.; Palmer, Jonathan M.; Vanderwolf, Karen J.; Schmidt, Katie Z.; Verant, Michelle L.; Weller, Theodore J.; Blehert, David S.Attribution: Biology and Ecosystems, National Wildlife Health Center, Ecosystems, Fish & Wildlife Disease, United States of America
View Citation
Malassezia vespertilionis sp. nov.: a new cold-tolerant species of yeast isolated from bats
JM Lorch, JM Palmer, KJ Vanderwolf, KZ Schmidt… - Persoonia-Molecular Phylogeny and Evolution of Fungi, 2018
Year Published: 2017
Phylogenetics of a fungal invasion: Origins and widespread dispersal of white-nose syndrome
Globalization has facilitated the worldwide movement and introduction of pathogens, but epizoological reconstructions of these invasions are often hindered by limited sampling and insufficient genetic resolution among isolates. Pseudogymnoascus destructans, a fungal pathogen causing the epizootic of white-nose syndrome in North American bats...
Drees, Kevin P.; Lorch, Jeffrey M.; Puechmaille, Sebastein J.; Parise, Katy L.; Wibbelt, Gudrun; Hoyt, Joseph R.; Sun, Keping; Jargalsaikhan, Ariunbold; Dalannast, Munkhnast; Palmer, Jonathan M.; Linder, Daniel L.; Kilpatrick, Marm; Pearson, Talima; Keim, Paul S.; Blehert, David S.; Foster, Jeffrey T.Attribution: National Wildlife Health Center, Ecosystems
View Citation
Drees, K.P., J.M. Lorch, S.J. Puechmaille, K.L. Parise, G. Wibbelt, J.R. Hoyt, K. Sun, A. Jargalsaikhan, M. Dalannast, J.M. Palmer, D.L. Lindner, A.M. Kilpatrick, T. Pearson, P.S. Keim, D.S. Blehert, J.T. Foster. 2017. Phylogenetics of a fungal invasion: Origins and widespread dispersal of white-nose syndrome. mBio 8:e01941-17. doi:10.1128/mBio.01941-17. IP 091970
Pre-USGS Publications
Orville, A. M., L. Manning, D.S. Blehert, J.M. Studts, B.W. Matthews, B.G. Fox, and G.H. Chambliss. 2004. Crystallization and preliminary analysis of xenobiotic reductase A and ligand complexes from Pseudomonas putida II-B. Acta Crystallographica 60: 957-961.
Orville, A. M., L. Manning, D.S. Blehert, B.G. Fox, and G.H. Chambliss. 2004. Crystallization and preliminary analysis of xenobiotic reductase B from Pseudomonas fluorescens I-C. Acta Crystallographica 60: 1289-1291.
Blehert, D.S., R.J. Palmer, Jr., J.B. Xavier, J.S. Almeida, and P.E. Kolenbrander. 2003. Autoinducer-2 production by Streptococcus gordonii DL1 and the biofilm phenotype of a luxS mutant are influenced by nutritional conditions. Journal of Bacteriology 185: 4851-4860.
The role of coaggregation in oral biofilm formation
Kolenbrander, P.E., R.F. Lerud, D.S. Blehert, P.G. Egland, J.S. Foster, and R.J. Palmer, Jr. 2003. The role of coaggregation in oral biofilm formation. In V. O’Flaherty, A. Moran, P. Lens and P. Stoodley (eds.), Biofilms in Medicine, Industry and Environmental Biotechnology, IWA Publishing, London, UK. p. 32-46.
Kolenbrander, P.E., R.N. Andersen, D.S. Blehert, P.G. Egland, J.S. Foster, and R.J. Palmer, Jr. 2002. Communication among oral bacteria. Microbiology and Molecular Biology Reviews 66: 486-505.
Blehert, D.S., B.G. Fox, and G.H. Chambliss. 1999. Cloning and sequence analysis of two Pseudomonas flavoprotein xenobiotic reductases. Journal of Bacteriology 181: 6254-6263.
Blehert, D.S., K.L. Knoke, B.G. Fox, and G.H. Chambliss. 1997. Regioselectivity of nitroglycerin denitration by flavoprotein nitroester reductases purified from two Pseudomonas species. Journal of Bacteriology 179: 6912-6920.
Date published: June 1, 2017
Alabama Survey Finds First Southeastern Bat with White-Nose Syndrome
Biologists have confirmed white-nose syndrome in the southeastern bat, or Myotis austroriparius, for the first time. The species joins eight other hibernating bat species in North America that are afflicted with the deadly bat fungal disease.
Date published: September 27, 2016
Newt News: Deadly Fish Disease Discovered in Salamanders
Attribution: Ecosystems, National Wildlife Health Center
Date published: March 31, 2016
Bat with white-nose syndrome confirmed in Washington state
OLYMPIA, Wash. – White-nose syndrome (WNS) has been confirmed in a little brown bat (Myotis lucifugus) found near North Bend – the first recorded occurrence of this devastating bat disease in western North America. The presence of this disease was verified by the U.S. Geological Survey’s National Wildlife Health Center
Date published: May 29, 2014
Ultra-violet Light Works as Screening Tool for Bats with White-nose Syndrome.
Scientists working to understand the devastating bat disease known as white-nose syndrome (WNS) now have a new, non-lethal tool to identify bats with WNS lesions —ultraviolet, or UV, light.