Isabelle M Cozzarelli, Ph.D.
Isabelle Cozzarelli is a Research Hydrologist with the USGS Geology, Energy & Minerals (GEM) Science Center in Reston, VA.
Biography
Isabelle uses a combined field and laboratory experimental approach to investigate biogeochemical processes in shallow groundwater and surface water environments that affect water availability. She uses a multi-disciplinary and multi-scale approach to study processes in environments stressed by anthropogenic inputs of biodegradable carbon, such as crude oil, landfill leachate, and oil and gas wastes.
Education
- Ph.D. University of Virginia, Environmental Sciences-Geochemistry, 1993
- M.S. University of Virginia, Environmental Sciences-Geochemistry, 1986
- B.S. University of Rochester, Geomechanics, 1983
Work Experience
- 2020-present: Research Hydrologist, USGS, Geology, Energy & Minerals Science Center, Reston, VA, USA
- 2017-2020: Research Hydrologist, USGS Water Mission Area Headquarters, Reston, VA, USA
- 1985-2017: Research Hydrologist, USGS National Research Program, USGS, Reston, Virginia, USA
- 1983-1985: Research Assistant, University of Virginia, Charlottesville, Virginia, USA
Professional Affiliations
- 2016-present: Adjunct Faculty, Department of Geosciences, Virginia Tech
Awards
- 2017: USGS Meritorious Service Award
- 2005: Elected GSA Fellow
- 2005: USGS Superior Service Award
Work Assignment
As a research hydrologist in the Geology, Energy, and Minerals Science Center I conduct long-term research on the fate and geochemical effect of organic contaminants in surface and subsurface environments. I use a combined field and laboratory approach in a variety of hydrogeologic environments in order to meet these objectives. I am currently a principal investigator for projects at unconventional oil and gas (UOG) drilling and disposal sites, the Bemidji, MN crude oil spill site and landfill-leachate and wastewater affected sites. My research at these sites focuses on the long-term evolution of biogeochemical zones in the aquifers and the natural attenuation of contaminants. The impact of chemical heterogeneity and the availability of electron acceptors on the extent of biodegradation have been a major thrust of my work. I have also studied aquifers affected by creosote and gasoline releases, with an emphasis on understanding the fate of metabolites in shallow aquifers.
List of Publications:
https://scholar.google.com/citations?hl=en&user=2X_djEgAAAAJ&view_op=lis...
Science and Products
Geochemical Signatures of Oil and Gas Wastewater from an Accidental Release Detected in Stream Sediment and Pore Waters Two Years Post Spill
Scientists identified geochemical signatures of wastewater in Blacktail Creek, North Dakota, as a result of a 2015 pipeline leak. They demonstrated that environmental signatures from wastewater spills are persistent within a short (1-3 year) time frame, can enter the creek through subsurface pathways, and can create the potential for extended environmental exposures.
Stormwater and Green Infrastructure
The Reston Biogeochemical Processes in Groundwater Laboratory (RBPGL) is conducting research aimed at understanding the complexity of chemical mixtures in urban stormwater.
Framework for Examining Stream Ecosystem Health in Areas of Shale Gas Development—A Multi-Parameter Watershed-Based Case Study in Pennsylvania
In a case study of 25 headwater streams in Pennsylvania, no statistically significant associations were determined between shale gas development and geochemical tracers of produced waters or measures of microbial and macroinvertebrate community composition. Although the results are specific to the region studied, the integrated biological and geochemical framework provides a tool for...
Produced water from Marcellus Shale and amphibians
Research biologists at the USGS Patuxent Wildlife Research Center (PWRC) are conducting a series of scientific studies on the potential effects of wastewater from hydraulic fracturing activities on terrestrial wildlife. Findings will help in assessing proposals for managing recycling and distribution of large volumes of flowback and produced waters generated by methods of oil and gas...
Wastewaters from Unconventional Oil and Gas Development
The Reston Biogeochemical Processes in Groundwater Laboratory (RBPGL) is assessing the potential risks to human and ecosystem health associated with Unconventional Oil and Gas (UOG) development.
Hydrocarbons and Biofuels
Reston Biogeochemical Processes in Groundwater Laboratory (RBPGL) research on hydrocarbons and biofuels is aimed at understanding the controls on rates of hydrocarbon biodegradation and secondary effects in the shallow subsurface and at the mixing interface between groundwater and surface water bodies such as wetlands.
Ongoing Research to Characterize the Complexity of Chemical Mixtures in Water Resources—Urban Stormwater
A multiagency reconnaissance study of chemicals in urban stormwater, sampled from pipes or ditches during 50 runoff events at 21 sites in 17 states across the United States, demonstrated that stormwater runoff contains complex mixtures of chemicals including polycyclic aromatic hydrocarbons, pesticides, and pharmaceuticals that are indicative of multiple sources in the watershed.
Energy Integrated Science Team
The Energy Integrated Science Team focuses on the potential for contaminant exposures in the environment that might originate from energy resource activities including, transportation, storage, extraction, waste management and restoration. Perceived health risks to humans and other organisms will be distinguished from actual risks, if any. If actual risks are identified...
Cyclical Mobilization and Attenuation of Naturally Occurring Arsenic in an Underground Petroleum Plume
Scientists found that naturally occurring arsenic in aquifer sediments was mobilized into groundwater and attenuated through reattachment to sediments within an underground petroleum plume. Understanding these patterns identifies anthropogenic factors that affect arsenic presence and magnitude in groundwater.
Landfills
The Reston Biogeochemical Processes in Groundwater Laboratory (RPBGL) studies landfill leachate biogeochemistry and the natural attenuation of contaminants in leachate plumes.
Program Scientist Receives Meritorious Service Award
Dr. Isabelle M. Cozzarelli received the U.S. Department of Interior's second highest honorary award—the Meritorious Service Award—for her numerous contributions to understanding the biogeochemical controls of contaminant degradation in groundwater and near-surface environments.
Examining Shifts in Stream Microbial Communities Exposed to Oil and Gas Wastewaters
Shifts in the overall microbial community structure were present in stream sediments that contained chemicals associated with unconventional oil and gas wastewaters. This work is part of a long-term study designed to understand persistence of chemicals from oil and gas wastewaters in sediments and water and how those factors might be related to exposures and adverse health effects, if any, on...
Arsenic in petroleum-contaminated groundwater near Bemidji, Minnesota is predicted to persist for centuries
We used a reactive transport model to investigate the cycling of geogenic arsenic (As) in a petroleum-contaminated aquifer. We simulated As mobilization and sequestration using surface complexation reactions with Fe(OH)3 during petroleum biodegradation coupled with Fe-reduction. Model results predict that dissolved As in the plume will exceed...
Ziegler, Brady A.; Ng, G.-H. Crystal; Cozzarelli, Isabelle M.; Dunshee, Aubrey J.; Schreiber, Madeline E. Arsenic release to the environment from hydrocarbon production, storage, transportation, use and waste management
Arsenic (As) is a toxic trace element with many sources, including hydrocarbons such as oil, natural gas, oil sands, and oil- and gas-bearing shales. Arsenic from these hydrocarbon sources can be released to the environment through human activities of hydrocarbon production, storage, transportation and use. In addition, accidental release of...
Schreiber, Madeline; Cozzarelli, Isabelle M. Geochemical and geophysical indicators of oil and gas wastewater can trace potential exposure pathways following releases to surface waters
Releases of oil and gas (OG) wastewaters can have complex effects on stream-water quality and downstream organisms, due to sediment-water interactions and groundwater/surface water exchange. Previously, elevated concentrations of sodium (Na), chloride (Cl), barium (Ba), strontium (Sr), and lithium (Li), and trace hydrocarbons were determined to be...
Cozzarelli, Isabelle M.; Kent, Douglas B.; Briggs, Martin A.; Engle, Mark A; Benthem, Adam; Skalak, Katherine; Mumford, Adam; Jaeschke, Jeanne B.; Farag, Aida; Lane, Jr., John W.; Akob, Denise M. Hydrocarbons to carboxyl-rich alicyclic molecules: A continuum model to describe biodegradation of petroleum-derived dissolved organic matter in contaminated groundwater plumes
Relationships between dissolved organic matter (DOM) reactivity and chemical composition in a groundwater plume containing petroleum-derived DOM (DOMHC) were examined by quantitative and qualitative measurements to determine the source and chemical composition of the compounds that persist downgradient. Samples were collected from a transect down...
Podgorski, David C.; Zito, Phoebe; Kellerman, Anne M.; Bekins, Barbara A.; Cozzarelli, Isabelle M.; Smith, Donald F.; Cao, Xiaoyan; Schmidt-Rohr, Klaus; Wagner, Sasha; Stubbins, Aron; Spencer, Robert G. M. Biological effects of hydrocarbon degradation intermediates: Is the total petroleum hydrocarbon analytical method adequate for risk assessment?
In crude oil contaminant plumes, the dissolved organic carbon (DOC) is mainly hydrocarbon degradation intermediates only partly quantified by the diesel range total petroleum hydrocarbon (TPHd) method. To understand potential biological effects of degradation intermediates, we tested three fractions of DOC: (1) solid-phase extract (HLB); (2)...
Bekins, Barbara A.; Brennan, Jennifer; Tillitt, Donald E.; Cozzarelli, Isabelle M.; Illig, Jennifer M.; Martinovich-Weigelt, Dalma Landfill leachate contributes per-/poly-fluoroalkyl substances (PFAS) and pharmaceuticals to municipal wastewater
Widespread disposal of landfill leachate to municipal sewer infrastructure in the United States calls for an improved understanding of the relative organic-chemical contributions to the wastewater treatment plant (WWTP) waste stream and associated surface-water discharge to receptors in the environment. Landfill leachate, WWTP influent, and WWTP...
Masoner, Jason R.; Kolpin, Dana W.; Cozzarelli, Isabelle M.; Smalling, Kelly L.; Bolyard, Stephanie; Field, Jennifer; Furlong, Edward T.; Gray, James L.; Lozinski, Duncan; Reinhart, Debra; Rodowa, Alix; Bradley, Paul M. Shale gas development has limited effects on stream biology and geochemistry in a gradient-based, multiparameter study in Pennsylvania
The number of horizontally drilled shale oil and gas wells in the United States has increased from nearly 28,000 in 2007 to nearly 127,000 in 2017, and research has suggested the potential for the development of shale resources to affect nearby stream ecosystems. However, the ability to generalize current studies is limited by the small geographic...
Mumford, Adam; Maloney, Kelly O.; Akob, Denise M.; Nettemann, Sarah; Proctor, Arianne; Ditty, Jason; Ulsamer, Luke; Lookenbill, Josh; Cozzarelli, Isabelle M. Urban stormwater: An overlooked pathway of extensive mixed contaminants to surface and groundwaters in the United States
Increasing global reliance on stormwater control measures to reduce discharge to surface water, increase groundwater recharge, and minimize contaminant delivery to receiving waterbodies necessitates improved understanding of stormwater-contaminant profiles. A multi-agency study of organic and inorganic chemicals in urban stormwater from 50 runoff...
Masoner, Jason R.; Kolpin, Dana W.; Cozzarelli, Isabelle M.; Barber, Larry B.; Burden, D.S.; Foreman, William T.; Forshay, Kenneth J.; Furlong, Edward; Groves, Justin F.; Hladik, Michelle; Hopton, Matthew E.; Jaeschke, Jeanne B.; Keefe, Steffanie H.; Krabbenhoft, David; Lowrance , Richard; Romanok, Kristin; Rus, David L.; Selbig, William R.; Williams, Brad; Bradley, Paul Predicting attenuation of salinized surface- and groundwater-resources from legacy energy development in the Prairie Pothole Region
Oil and gas (energy) development in the Williston Basin, which partly underlies the Prairie Pothole Region in central North America, has helped meet U.S. energy demand for decades. Historical handling and disposal practices of saline wastewater co-produced during energy development resulted in salinization of surface and groundwater at numerous...
Preston, Todd M.; Anderson, Chauncey W.; Thamke, Joanna N.; Hossack, Blake R.; Skalak, Katherine; Cozzarelli, Isabelle M. Biological effects of elevated major ions in surface water contaminated by a produced water from oil production
Produced water (PW) from oil and gas extraction processes has been shown to contain elevated concentrations of major ions. The objective of this study was to determine the potential effects of elevated major ions in PW-contaminated surface water on a fish (fathead minnow, Pimephales promelas) and a unionid mussel (fatmucket, Lampsilis siliquoidea...
Wang, Ning; Kunz, James L.; Cleveland, Danielle; Steevens, Jeffery; Cozzarelli, Isabelle M. Associations between environmental pollutants and larval amphibians in wetlands contaminated by energy-related brines are potentially mediated by feeding traits
Energy production in the Williston Basin, located in the Prairie Pothole Region of central North America, has increased rapidly over the last several decades. Advances in recycling and disposal practices of saline wastewaters (brines) co-produced during energy production have reduced ecological risks, but spills still...
Smalling, Kelly L.; Anderson, Chauncey W.; Honeycutt, R. Ken; Cozzarelli, Isabelle M.; Preston, Todd M.; Hossack, Blake R. Toxicity assessment of groundwater contaminated by petroleum hydrocarbons at a well-characterized, aged, crude oil release site
Management of petroleum-impacted waters by monitored natural attenuation requires an understanding of the toxicology of both the original compounds released and the transformation products formed during natural breakdown. Here, we report data from a groundwater plume consisting of a mixture of crude oil compounds and transformation products in an...
McGuire, Jennifer T.; Cozzarelli, Isabelle M.; Bekins, Barbara A.; Link, Hannah; Martinović-Weigelt, Dalma
Energy Integrated Science Team
The Energy Integrated Science Team focuses on the potential for contaminant exposures in the environment that might originate from energy resource activities including, transportation, storage, extraction, waste management and restoration. Perceived health risks to humans and other organisms will be distinguished from actual risks, if any.
Cyclical Mobilization and Attenuation of Naturally Occurring Arsenic in an Underground Petroleum Plume
Scientists found that naturally occurring arsenic in aquifer sediments was mobilized into groundwater and attenuated through reattachment to sediments within an underground petroleum plume. Understanding these patterns identifies anthropogenic factors that affect arsenic presence and magnitude in groundwater.
Ongoing Research to Characterize the Complexity of Chemical Mixtures in Water Resources—Urban Stormwater
A multiagency reconnaissance study of chemicals in urban stormwater, sampled from pipes or ditches during 50 runoff events at 21 sites in 17 states across the United States, demonstrated that stormwater runoff contains complex mixtures of chemicals including polycyclic aromatic hydrocarbons, pesticides, and pharmaceuticals that are indicative of multiple sources in the watershed.
Framework for Examining Stream Ecosystem Health in Areas of Shale Gas Development—A Multi-Parameter Watershed-Based Case Study in Pennsylvania
In a case study of 25 headwater streams in Pennsylvania, no statistically significant associations were determined between shale gas development and geochemical tracers of produced waters or measures of microbial and macroinvertebrate community composition.
Department of Interior Secretary Ryan Zinke Visits with Scientists from the Reston Biogeochemical Processes in Groundwater Lab
Department of Interior Secretary Ryan Zinke recently visited Reston to share his vision for the U.S. Geological Survey. He visited with USGS personnel and stopped by the Reston Biogeochemical Processes in Groundwater and Reston Microbiology Laboratories to learn more about what we do.
Evidence of Unconventional Oil and Gas Wastewater Found in Surface Waters near Underground Injection Site
These are the first published studies to demonstrate water-quality impacts to a surface stream due to activities at an unconventional oil and gas wastewater deep well injection disposal site.
Crude Oil Byproducts in Groundwater Plumes
A new study suggests that the degraded breakdown products of oil-spill contaminants in groundwater could be just as important to monitor as the original contamination itself.
Storage and Treatment of Liquid Waste from Landfills Doesn’t Remove All Contaminants, Including Pharmaceuticals
New research from the U.S. Geological Survey details that even after the storage and/or treatment of leachate – liquid waste that moves through or drains from a landfill − it can still contain a multitude of chemicals and reflects the diverse nature of residential, industrial, and commercial waste discarded into landfills in the United States.
The Chemistry of Waters that Follow from Fracking: A Case Study
In a study of 13 hydraulically fractured shale gas wells in north-central Pennsylvania, USGS researchers found that the microbiology and organic chemistry of the produced waters varied widely from well to well.
Natural Breakdown of Petroleum Underground Can Lace Arsenic into Groundwater
In a long-term field study, U.S. Geological Survey (USGS) and Virginia Tech scientists have found that changes in geochemistry from the natural breakdown of petroleum hydrocarbons underground can promote the chemical release (mobilization) of naturally occurring arsenic into groundwater. This geochemical change can result in potentially significant arsenic groundwater contamination.