USGS researchers (GLSC; Chesterton, Indiana) spiking chrysanthemums with DNA prior to a simulated rainfall as part of an environmental DNA degradation experiment. Photo credit: Zachary Hatfield, USGS Contractor.
Muruleedhara (Murulee) N Byappanahalli, PhD
I am a Staff Scientist (Research Microbiologist) with the USGS Great Lakes Science Center’s Lake Michigan Ecological Research Station in Chesterton, Indiana, conducting research in restoration and conservation biology programs, with projects in aquatic and terrestrial ecosystems. Emerging technologies, such as eDNA, high throughput sequencing, and microbiome, are routinely used in these projects.
My research at the Great Lakes Science Center’s (GLSC) Lake Michigan Ecological Research Station (GLSC-LMERS) spans across aquatic and terrestrial ecosystems, focusing on restoration and conservation biology programs. Environmental microbiology and environmental genomics are key disciplines in these projects.
In previous work, I studied the distribution and population characteristics of enteric bacteria in Great Lakes watersheds (streams and riparian soils, beach sand, aquatic vegetation) and their impacts on beneficial use impairments such as water quality. I also explored the best management practices to restore these degraded waterways.
My microbial research in terrestrial programs is a collaborative effort devoted to understanding the biology of plant invasion. Specifically, I am exploring how soil microorganisms (bacteria, fungi) might augment or diminish this invasive process, using the Asiatic bittersweet (Celastrus orbiculatus) as a model plant species. Much of this his research is conducted in public lands within the Indiana Dunes National Park.
My research in environmental genomics, which is collaborative, brings an array of emerging technologies in support of ongoing fisheries assessment and conservation programs. I use environmental DNA (eDNA), autonomous sample processors, and high throughput sequencing (HTS) methods, including metabarcoding and shotgun sequencing, in conjunction with traditional surveys (e.g., trawling).
Recently, I have been applying eDNA and HTS tools to identify pollinator species (bees and bee communities) in grasslands from different geographical areas, by capturing eDNA left behind by pollinators visiting flowering plants. The main goal of this Ecosystem Mission Area (EMA) funded project, which involves multiple USGS Science Centers (including GLSC-LMERS), is to develop new DNA-based tools to improve existing monitoring programs for pollinators, including listed/endangered species.
Professional Experience
2001-Present: Research Microbiologist, Great Lakes Science Center, Lake Michigan Ecological Research Station, Chesterton, Indiana
Education and Certifications
Ph.D. (Microbiology, 2000): University of Hawaii at Manoa, Honolulu, Hawaii
MS (Microbiology, 1996): University of Hawaii at Manoa, Honolulu, Hawaii
MSc (Agricultural Microbiology, 1982): University of Agricultural Sciences, Bengaluru, India
BSc (Agriculture, 1979): University of Agricultural Sciences, Bengaluru, India
Affiliations and Memberships*
American Society for Microbiology (ASM), Washington, D.C.
Soil Science Society of America (SSSA), Madison, Wisconsin
Science and Products
Using Pollinator Environmental DNA to Assess the Ecological Resilience of America’s Grasslands
READI-Net: Providing Tools for the Early Detection and Management of Aquatic Invasive Species
Data releases by this scientist
Potential for microbially mediated nitrogen transformations in benthic algae, sediment, and overlying water in the Great Lakes, 2022 Potential for microbially mediated nitrogen transformations in benthic algae, sediment, and overlying water in the Great Lakes, 2022
Nuisance algae: Planktonic communities in the Grand Calumet River Area of Concern, Indiana, 2021 Nuisance algae: Planktonic communities in the Grand Calumet River Area of Concern, Indiana, 2021
Algal community composition and associated fish eDNA in southern Lake Michigan shoreline deposits (2024) Algal community composition and associated fish eDNA in southern Lake Michigan shoreline deposits (2024)
Detection of Apis mellifera DNA in spiked flowers under laboratory and natural conditions, Chesterton Indiana, 2023-2024 Detection of Apis mellifera DNA in spiked flowers under laboratory and natural conditions, Chesterton Indiana, 2023-2024
Round goby detection by scuba divers’ visual assessments and environmental DNA in the Great Lakes, 2022 Round goby detection by scuba divers’ visual assessments and environmental DNA in the Great Lakes, 2022
Acoustic Doppler Current Profiler data collected in near Hammond Bay, Lake Huron, 2019 Acoustic Doppler Current Profiler data collected in near Hammond Bay, Lake Huron, 2019
Microbial Communities Across a Successional Gradient at Indiana Dunes National Park, 2015-2017 Microbial Communities Across a Successional Gradient at Indiana Dunes National Park, 2015-2017
Grand Calumet River Area of Concern Metabarcoding Data Grand Calumet River Area of Concern Metabarcoding Data
Oriental Bittersweet Microbial Communities Across a Post Glacial Successional Gradient at Indiana Dunes National Park, 2017 Oriental Bittersweet Microbial Communities Across a Post Glacial Successional Gradient at Indiana Dunes National Park, 2017
Eutrophication and plankton communities (Cyanobacteria and eukaryotic algae) in the Grand Calumet River Area of Concern, Indiana, 2021 Eutrophication and plankton communities (Cyanobacteria and eukaryotic algae) in the Grand Calumet River Area of Concern, Indiana, 2021
Environmental DNA detection and survival, influence of sediment, and stream transport in a Lake Michigan watershed, 2018 Environmental DNA detection and survival, influence of sediment, and stream transport in a Lake Michigan watershed, 2018
Identify sources of high E. coli concentrations, beaches of southern Lake Michigan, 2015, (version 2.0, July 2020) Identify sources of high E. coli concentrations, beaches of southern Lake Michigan, 2015, (version 2.0, July 2020)
Multimedia related to this scientist
USGS researchers (GLSC; Chesterton, Indiana) spiking chrysanthemums with DNA prior to a simulated rainfall as part of an environmental DNA degradation experiment. Photo credit: Zachary Hatfield, USGS Contractor.
Flower sampling in the GLSC greenhouse in Chesterton, Indiana for simulated environmental DNA degradation experiments. Photo credit: Muruleedhara Byappanahalli, USGS.
Flower sampling in the GLSC greenhouse in Chesterton, Indiana for simulated environmental DNA degradation experiments. Photo credit: Muruleedhara Byappanahalli, USGS.
USGS scientists busy developing methods for identifying pollinators using environmental-DNA. Photo credit: Dawn Shively, USGS (contractor).
USGS scientists busy developing methods for identifying pollinators using environmental-DNA. Photo credit: Dawn Shively, USGS (contractor).
Aaron Aunins (Eastern Ecological Science Center, Leetown, West Virginia) prepares samples for polymerase chain reaction (PCR) as Kasia Kelly (left) and Dawn Shively (middle) (both from Great Lakes Science Center, Chesterton, Indiana) observe and document the procedure. Photo credit: Murulee Byappanahalli, USGS.
Aaron Aunins (Eastern Ecological Science Center, Leetown, West Virginia) prepares samples for polymerase chain reaction (PCR) as Kasia Kelly (left) and Dawn Shively (middle) (both from Great Lakes Science Center, Chesterton, Indiana) observe and document the procedure. Photo credit: Murulee Byappanahalli, USGS.
Publications by this scientist
Bacterial community structure across a sand dune chronosequence at the Indiana Dunes National Park Bacterial community structure across a sand dune chronosequence at the Indiana Dunes National Park
Bacteria common to rhizosphere communities of Asiatic bittersweet across a post-glacial landscape Bacteria common to rhizosphere communities of Asiatic bittersweet across a post-glacial landscape
Microbial source tracking and evaluation of best management practices for restoring degraded beaches of Lake Michigan Microbial source tracking and evaluation of best management practices for restoring degraded beaches of Lake Michigan
Gut microbiota associated with different sea lamprey (Petromyzon marinus) life stages Gut microbiota associated with different sea lamprey (Petromyzon marinus) life stages
Influence of filter pore size on composition and relative abundance of bacterial communities and select host-specific MST markers in coastal waters of southern Lake Michigan Influence of filter pore size on composition and relative abundance of bacterial communities and select host-specific MST markers in coastal waters of southern Lake Michigan
Sequestration of microfibers and other microplastics by green algae, Cladophora, in the US Great Lakes Sequestration of microfibers and other microplastics by green algae, Cladophora, in the US Great Lakes
Influence of sediment and stream transport on detecting a source of environmental DNA Influence of sediment and stream transport on detecting a source of environmental DNA
Evaluating the impacts of foreshore sand and birds on microbiological contamination at a freshwater beach Evaluating the impacts of foreshore sand and birds on microbiological contamination at a freshwater beach
Utilization of multiple microbial tools to evaluate efficacy of restoration strategies to improve recreational water quality at a Lake Michigan Beach (Racine, WI) Utilization of multiple microbial tools to evaluate efficacy of restoration strategies to improve recreational water quality at a Lake Michigan Beach (Racine, WI)
Interaction of bacterial communities and indicators of water quality in shoreline sand, sediment, and water of Lake Michigan Interaction of bacterial communities and indicators of water quality in shoreline sand, sediment, and water of Lake Michigan
Quantifying microplastics in Indiana’s Lake Michigan watershed and submerged aquatic vegetation Quantifying microplastics in Indiana’s Lake Michigan watershed and submerged aquatic vegetation
Great Lakes Cladophora harbors phylogenetically diverse nitrogen-fixing microorganims Great Lakes Cladophora harbors phylogenetically diverse nitrogen-fixing microorganims
Non-USGS Publications**
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
News about this scientist
Science and Products
Using Pollinator Environmental DNA to Assess the Ecological Resilience of America’s Grasslands
READI-Net: Providing Tools for the Early Detection and Management of Aquatic Invasive Species
Data releases by this scientist
Potential for microbially mediated nitrogen transformations in benthic algae, sediment, and overlying water in the Great Lakes, 2022 Potential for microbially mediated nitrogen transformations in benthic algae, sediment, and overlying water in the Great Lakes, 2022
Nuisance algae: Planktonic communities in the Grand Calumet River Area of Concern, Indiana, 2021 Nuisance algae: Planktonic communities in the Grand Calumet River Area of Concern, Indiana, 2021
Algal community composition and associated fish eDNA in southern Lake Michigan shoreline deposits (2024) Algal community composition and associated fish eDNA in southern Lake Michigan shoreline deposits (2024)
Detection of Apis mellifera DNA in spiked flowers under laboratory and natural conditions, Chesterton Indiana, 2023-2024 Detection of Apis mellifera DNA in spiked flowers under laboratory and natural conditions, Chesterton Indiana, 2023-2024
Round goby detection by scuba divers’ visual assessments and environmental DNA in the Great Lakes, 2022 Round goby detection by scuba divers’ visual assessments and environmental DNA in the Great Lakes, 2022
Acoustic Doppler Current Profiler data collected in near Hammond Bay, Lake Huron, 2019 Acoustic Doppler Current Profiler data collected in near Hammond Bay, Lake Huron, 2019
Microbial Communities Across a Successional Gradient at Indiana Dunes National Park, 2015-2017 Microbial Communities Across a Successional Gradient at Indiana Dunes National Park, 2015-2017
Grand Calumet River Area of Concern Metabarcoding Data Grand Calumet River Area of Concern Metabarcoding Data
Oriental Bittersweet Microbial Communities Across a Post Glacial Successional Gradient at Indiana Dunes National Park, 2017 Oriental Bittersweet Microbial Communities Across a Post Glacial Successional Gradient at Indiana Dunes National Park, 2017
Eutrophication and plankton communities (Cyanobacteria and eukaryotic algae) in the Grand Calumet River Area of Concern, Indiana, 2021 Eutrophication and plankton communities (Cyanobacteria and eukaryotic algae) in the Grand Calumet River Area of Concern, Indiana, 2021
Environmental DNA detection and survival, influence of sediment, and stream transport in a Lake Michigan watershed, 2018 Environmental DNA detection and survival, influence of sediment, and stream transport in a Lake Michigan watershed, 2018
Identify sources of high E. coli concentrations, beaches of southern Lake Michigan, 2015, (version 2.0, July 2020) Identify sources of high E. coli concentrations, beaches of southern Lake Michigan, 2015, (version 2.0, July 2020)
Multimedia related to this scientist
USGS researchers (GLSC; Chesterton, Indiana) spiking chrysanthemums with DNA prior to a simulated rainfall as part of an environmental DNA degradation experiment. Photo credit: Zachary Hatfield, USGS Contractor.
USGS researchers (GLSC; Chesterton, Indiana) spiking chrysanthemums with DNA prior to a simulated rainfall as part of an environmental DNA degradation experiment. Photo credit: Zachary Hatfield, USGS Contractor.
Flower sampling in the GLSC greenhouse in Chesterton, Indiana for simulated environmental DNA degradation experiments. Photo credit: Muruleedhara Byappanahalli, USGS.
Flower sampling in the GLSC greenhouse in Chesterton, Indiana for simulated environmental DNA degradation experiments. Photo credit: Muruleedhara Byappanahalli, USGS.
USGS scientists busy developing methods for identifying pollinators using environmental-DNA. Photo credit: Dawn Shively, USGS (contractor).
USGS scientists busy developing methods for identifying pollinators using environmental-DNA. Photo credit: Dawn Shively, USGS (contractor).
Aaron Aunins (Eastern Ecological Science Center, Leetown, West Virginia) prepares samples for polymerase chain reaction (PCR) as Kasia Kelly (left) and Dawn Shively (middle) (both from Great Lakes Science Center, Chesterton, Indiana) observe and document the procedure. Photo credit: Murulee Byappanahalli, USGS.
Aaron Aunins (Eastern Ecological Science Center, Leetown, West Virginia) prepares samples for polymerase chain reaction (PCR) as Kasia Kelly (left) and Dawn Shively (middle) (both from Great Lakes Science Center, Chesterton, Indiana) observe and document the procedure. Photo credit: Murulee Byappanahalli, USGS.
Publications by this scientist
Bacterial community structure across a sand dune chronosequence at the Indiana Dunes National Park Bacterial community structure across a sand dune chronosequence at the Indiana Dunes National Park
Bacteria common to rhizosphere communities of Asiatic bittersweet across a post-glacial landscape Bacteria common to rhizosphere communities of Asiatic bittersweet across a post-glacial landscape
Microbial source tracking and evaluation of best management practices for restoring degraded beaches of Lake Michigan Microbial source tracking and evaluation of best management practices for restoring degraded beaches of Lake Michigan
Gut microbiota associated with different sea lamprey (Petromyzon marinus) life stages Gut microbiota associated with different sea lamprey (Petromyzon marinus) life stages
Influence of filter pore size on composition and relative abundance of bacterial communities and select host-specific MST markers in coastal waters of southern Lake Michigan Influence of filter pore size on composition and relative abundance of bacterial communities and select host-specific MST markers in coastal waters of southern Lake Michigan
Sequestration of microfibers and other microplastics by green algae, Cladophora, in the US Great Lakes Sequestration of microfibers and other microplastics by green algae, Cladophora, in the US Great Lakes
Influence of sediment and stream transport on detecting a source of environmental DNA Influence of sediment and stream transport on detecting a source of environmental DNA
Evaluating the impacts of foreshore sand and birds on microbiological contamination at a freshwater beach Evaluating the impacts of foreshore sand and birds on microbiological contamination at a freshwater beach
Utilization of multiple microbial tools to evaluate efficacy of restoration strategies to improve recreational water quality at a Lake Michigan Beach (Racine, WI) Utilization of multiple microbial tools to evaluate efficacy of restoration strategies to improve recreational water quality at a Lake Michigan Beach (Racine, WI)
Interaction of bacterial communities and indicators of water quality in shoreline sand, sediment, and water of Lake Michigan Interaction of bacterial communities and indicators of water quality in shoreline sand, sediment, and water of Lake Michigan
Quantifying microplastics in Indiana’s Lake Michigan watershed and submerged aquatic vegetation Quantifying microplastics in Indiana’s Lake Michigan watershed and submerged aquatic vegetation
Great Lakes Cladophora harbors phylogenetically diverse nitrogen-fixing microorganims Great Lakes Cladophora harbors phylogenetically diverse nitrogen-fixing microorganims
Non-USGS Publications**
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
News about this scientist
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government