Luke R Iwanowicz, Ph.D. (Former Employee)
Science and Products
Filter Total Items: 27
Estrogen equivalents of surface water in Pennsylvania, 2012-2017 Estrogen equivalents of surface water in Pennsylvania, 2012-2017
The data were gathered as part of a long-term, seasonal assessment of estrogenicity in selected locations within Pennsylvania from 2012-2017. Data were collected over this time frame to determine seasonal and annual changes in surface water estrogenicity.Estrogen is reported as estrogen equivalents (EEQ) as determined by a bioluminescent yeast estrogen screen. A total of 193 grab water...
Data supporting: Draft Genome Sequence of a Novel Calicivirus from a Brown Bullhead (Ameiurus nebulosus) from Lake Memphremagog, VT Data supporting: Draft Genome Sequence of a Novel Calicivirus from a Brown Bullhead (Ameiurus nebulosus) from Lake Memphremagog, VT
We report a draft genome of a previously undescribed calicivirus from a single brown bullhead inhabiting Lake Memphremagog, Vermont/Quebec. The genome is 7413 nt in length and is most similar to the Atlantic salmon calicivirus (nucleotide identity: 64.7%).
Current use pesticides in larval amphibian tissues, amphibian pathogen and wetland sediment screening data from three northeastern National Wildlife Refuges, 2013-2014 Current use pesticides in larval amphibian tissues, amphibian pathogen and wetland sediment screening data from three northeastern National Wildlife Refuges, 2013-2014
The data include concentrations of current use pesticides in tissues of larval wood frog (Lithobates sylvaticus) and spotted salamander (Ambystoma maculatum) and the presence of ranavirus in wood frogs and spotted salamanders from three northeastern National Wildlife Refuges sampled in 2013 and 2014. The data also include estrogenicity, protein phosphatase 2A inhibition and a suite of 15...
Water-quality data for a pharmaceutical study at Muddy Creek in North Liberty and Coralville, Iowa, 2017-2018 Water-quality data for a pharmaceutical study at Muddy Creek in North Liberty and Coralville, Iowa, 2017-2018
Samples were collected, processed, and analyzed for organics, estrogen equivalents, and fecal indicator bacteria. Filtered organic samples were sent to the National Water Quality Laboratory in Denver, Colorado. Unfiltered estrogen equivalent samples were sent to the Organic Geochemistry Research Lab in Lawrence, Kansas, for extraction, after which they were sent to the National Fish...
2004-2010 Chesapeake Bay Smallmouth and Largemouth Bass Estrogenic Biomarker Data 2004-2010 Chesapeake Bay Smallmouth and Largemouth Bass Estrogenic Biomarker Data
The data was gathered to document the extent and severity of biomarkers of exposure to estrogenic chemicals in smallmouth and largemouth bass. Samples were collected from 2004 through 2010 at sites in five watersheds within the Chesapeake Bay watershed. A total of 281 largemouth bass were collected from the Potomac, Susquehanna, Patuxent and Rappahannock rivers and 1,384 smallmouth bass...
Estrogen equivalents of surface water and smallmouth bass estrogenic biomarker data in New Jersey, 2016-2017 Estrogen equivalents of surface water and smallmouth bass estrogenic biomarker data in New Jersey, 2016-2017
The data were gathered as a preliminary assessment of estrogenicity under base-flow conditions at over 100 sites (lakes and streams) throughout New Jersey followed by more targeted sampling of smallmouth bass at nine sites with varying levels of estrogenicity. In 2016, 102 sites (lakes and streams) for the preliminary estrogenicity assessment were selected utilizing compiled results of...
Microbial and chemical contaminant occurrence and concentration in groundwater and surface water proximal to large-scale poultry facilities and poultry litter, 2016 Microbial and chemical contaminant occurrence and concentration in groundwater and surface water proximal to large-scale poultry facilities and poultry litter, 2016
Chemical and biological results, quality assurance and quality control, and method information from groundwater, surface water, and litter samples, collected from nine locations in Iowa and one in Wisconsin in 2016. Thirteen groundwater, nine surface water, four poultry litter, and four QA/QC samples were collected. Samples were analyzed at U.S. Geological Survey laboratories; bacteria...
Toxicity of potassium chloride, active compound in the molluscicide potash, on salmonid fishes and their forage base (Leetown Science Center, 2018). Toxicity of potassium chloride, active compound in the molluscicide potash, on salmonid fishes and their forage base (Leetown Science Center, 2018).
The data is supplemental to the 2018 USGS Open File Report, "An evaluation of the acute and chronic toxicity of potassium chloride, active compound in the molluscicide potash, on salmonid fishes and their forage base". This research was performed as a Science Support Partnership (SSP) study with USFWS in order to evaluate potential lethal and sublethal impacts of potassium chloride on...
Molecular detection of avian influenza virus from sediment samples in waterfowl habitats on the Delmarva Peninsula, USA Molecular detection of avian influenza virus from sediment samples in waterfowl habitats on the Delmarva Peninsula, USA
Data were collected as part of an investigation developed by Leetown Science Center to investigate the comparative detection of avian influenza viruses from waterfowl and potential environmental reservoirs such as aquatic sediment from waterfowl habitat. This dataset identifies positive or negative test results for qRT-PCR (quantitative reverse transcriptase polymerase chain reaction)...
Estrogen Equivalents of Surface Water in the Upper Chesapeake Bay Watershed (2008 - 2011) Estrogen Equivalents of Surface Water in the Upper Chesapeake Bay Watershed (2008 - 2011)
Intersex in small- and largemouth bass has been observed in the upper Chesapeake Bay Watershed since 2003. This observation is indicative of exposure to estrogenic endocrine disrupting chemicals. In order to complement biological data sets and better identify the spatial and temporal nature of this end-point, discrete grab water samples were collected throughout the upper Chesapeake Bay...
Chelonid Herpesvirus 5 (ChHV5) genomic sequences obtained through long-range PCR and high throughput sequencing from Florida and Hawaii green sea turtles with fibropapillomatosis Chelonid Herpesvirus 5 (ChHV5) genomic sequences obtained through long-range PCR and high throughput sequencing from Florida and Hawaii green sea turtles with fibropapillomatosis
The files in this data release are the processed DNA consensus sequence alignment files for the chelonid herpesvirus 5 (ChHV-5) genome plus 66 open reading frames representing protien-coding genes. DNA was extracted from tumor biopsies from green sea turtles (Chelonias mydas) with fibropapillomatosis sampled from Florida and Hawaii coastal waters. We used high-throughput short-read...
Analytical inorganic chemistry results for samples collected for the Sediment-Bound Contaminant Resiliency and Response Strategy pilot study, northeastern United States, 2015 Analytical inorganic chemistry results for samples collected for the Sediment-Bound Contaminant Resiliency and Response Strategy pilot study, northeastern United States, 2015
Coastal communities are uniquely vulnerable to sea-level rise (SLR) and severe storms such as hurricanes. These events enhance the dispersion and concentration of natural and anthropogenic chemicals and pathogenic microorganisms that could adversely affect the health and resilience of coastal communities and ecosystems in coming years. The U.S. Geological Survey has developed the...
Filter Total Items: 110
Transcriptome signatures of wastewater effluent exposure in larval zebrafish vary with seasonal mixture composition in an effluent-dominated stream Transcriptome signatures of wastewater effluent exposure in larval zebrafish vary with seasonal mixture composition in an effluent-dominated stream
Wastewater treatment plant (WWTP) effluent-dominated streams provide critical habitat for aquatic and terrestrial organisms but also continually expose them to complex mixtures of pharmaceuticals that can potentially impair growth, behavior, and reproduction. Currently, few biomarkers are available that relate to pharmaceutical-specific mechanisms of action. In the experiment reported in...
Authors
Emma B. Meade, Luke R. Iwanowicz, Nicklaus Neureuther, Gregory H. LeFevre, Dana W. Kolpin, Hui Zhi, Shannon M. Meppelink, Rachael F. Lane, Angela Schmoldt, Aurash Mohaimani, Olaf Mueller, Rebecca D. Klaper
Using biological responses to monitor freshwater post-spill conditions over 3 years in Blacktail Creek, North Dakota, USA Using biological responses to monitor freshwater post-spill conditions over 3 years in Blacktail Creek, North Dakota, USA
A pipeline carrying unconventional oil and gas (OG) wastewater spilled approximately 11 million liters of wastewater into Blacktail Creek, North Dakota, USA. Flow of the mix of stream water and wastewater down the channel resulted in storage of contaminants in the hyporheic zone and along the banks, providing a long-term source of wastewater constituents to the stream. A multi-level...
Authors
Aida Farag, David Harper, Isabelle M. Cozzarelli, Douglas B. Kent, Adam Mumford, Denise M. Akob, Travis W. Schaeffer, Luke R. Iwanowicz
By
Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, Columbia Environmental Research Center, Earth Resources Observation and Science (EROS) Center , Eastern Ecological Science Center, Geology, Energy & Minerals Science Center, Reston Microbiology Laboratory
RNA-seq reveals potential gene biomarkers in fathead minnows (Pimephales promelas) for exposure to treated wastewater effluent RNA-seq reveals potential gene biomarkers in fathead minnows (Pimephales promelas) for exposure to treated wastewater effluent
Discharged wastewater treatment plant (WWTP) effluent greatly contributes to the generation of complex mixtures of contaminants of emerging concern (CECs) in aquatic environments which often contain neuropharmaceuticals and other emerging contaminants that may impact neurological function. However, there is a paucity of knowledge on the neurological impacts of these exposures to aquatic...
Authors
Peter Schumann, E. Meade, H. Zhi, G. H. LeFevre, Dana W. Kolpin, Shannon M. Meppelink, Luke R. Iwanowicz, Rachael F. Lane, A. Schmoldt, O. Mueller, R. D. Klaper
Freshwater unionid mussels threatened by predation of Round Goby (Neogobius melanostomus) Freshwater unionid mussels threatened by predation of Round Goby (Neogobius melanostomus)
Indigenous freshwater mussels (Unionidae) are integral to riverine ecosystems, playing a pivotal role in aquatic food webs and providing ecological services. With populations on the decline worldwide, freshwater mussels are of conservation concern. In this study, we explore the propensity of the invasive Round Goby (Neogobius melanostomus) fish to prey upon indigenous freshwater mussels...
Authors
Kyle Clark, Deborah D. Iwanowicz, Luke R. Iwanowicz, Sara Mueller, Joshua Wisor, Casey Bradshaw-Wilson, W. Bane Schill, Jay R. Stauffer, Elizabeth W. Boyer
Draft genome sequence of a novel calicivirus from a brown bullhead (Ameiurus nebulosus) from Lake Memphremagog, Vermont/Quebec Draft genome sequence of a novel calicivirus from a brown bullhead (Ameiurus nebulosus) from Lake Memphremagog, Vermont/Quebec
We report a draft genome sequence of a previously undescribed calicivirus from a single brown bullhead inhabiting Lake Memphremagog, Vermont/Quebec. The genome is 7,413 nucleotides long and is most similar to the Atlantic salmon calicivirus (nucleotide identity; 64.7%).
Authors
Luke R. Iwanowicz, Vicki S. Blazer, Tom Jones, Matthew Bodnar, Korin Ekholm, Julie Dragon, Peter Emmerson
Site- and individual-level contaminations affect infection prevalence of an emerging infectious disease of amphibians Site- and individual-level contaminations affect infection prevalence of an emerging infectious disease of amphibians
Emerging infectious disease outbreaks are one of multiple stressors responsible for amphibian declines globally. In the northeastern United States, ranaviral diseases are prevalent in amphibians and other ectothermic species, but there is still uncertainty as to whether their presence is leading to population level effects. Further, there is also uncertainty surrounding the potential...
Authors
Kelly L. Smalling, Brittany A. Mosher, Luke R. Iwanowicz, Keith A. Loftin, Adam Boehlke, Michelle L. Hladik, Carly R. Muletz-Wolz, Nandadevi Cortes-Rodriguez, Robin Femmer, Evan H. Campbell Grant
By
Ecosystems Mission Area, Water Resources Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, Central Plains Water Science Center, Eastern Ecological Science Center, California Water Science Center, Geology, Geophysics, and Geochemistry Science Center, New Jersey Water Science Center
Tandem field and laboratory approaches to quantify attenuation mechanisms of pharmaceutical and pharmaceutical transformation products in a wastewater effluent-dominated stream Tandem field and laboratory approaches to quantify attenuation mechanisms of pharmaceutical and pharmaceutical transformation products in a wastewater effluent-dominated stream
Evolving complex mixtures of pharmaceuticals and transformation products in effluent-dominated streams pose potential impacts to aquatic species; thus, understanding the attenuation dynamics in the field and characterizing the prominent attenuation mechanisms of pharmaceuticals and their transformation products (TPs) is critical for hazard assessments. Herein, we determined the...
Authors
Hui Zhi, Alyssa L Mianecki, Dana W. Kolpin, Rebecca D. Klaper, Luke R. Iwanowicz, Gregory H. LeFevre
Pilot-scale expanded assessment of inorganic and organic tapwater exposures and predicted effects in Puerto Rico, USA Pilot-scale expanded assessment of inorganic and organic tapwater exposures and predicted effects in Puerto Rico, USA
A pilot-scale expanded target assessment of mixtures of inorganic and organic contaminants in point-of-consumption drinking water (tapwater, TW) was conducted in Puerto Rico (PR) to continue to inform TW exposures and corresponding estimations of cumulative human-health risks across the US. In August 2018, a spatial synoptic pilot assessment of than 524 organic, 37 inorganic, and select
Authors
Paul M. Bradley, Ingrid Y. Padilla, Kristin M. Romanok, Kelly Smalling, Michael J. Focazio, Sara E. Breitmeyer, Mary C. Cardon, Justin M. Conley, Nicola Evans, Carrie E. Givens, James L. Gray, L. Earl Gray, Phillip C. Hartig, Michelle L. Hladik, Christopher P. Higgins, Luke R. Iwanowicz, Rachael F. Lane, Keith A. Loftin, R. Blaine McCleskey, Carrie A. McDonough, Elizabeth Medlock-Kakaley, Shannon M. Meppelink, Christopher P. Weis, Vickie S. Wilson
Emerging investigator series: Municipal wastewater as a year-round point source of neonicotinoid insecticides that persist in an effluent-dominated stream Emerging investigator series: Municipal wastewater as a year-round point source of neonicotinoid insecticides that persist in an effluent-dominated stream
Neonicotinoids in aquatic systems have been predominantly associated with agriculture, but some are increasingly being linked to municipal wastewater. Thus, the aim of this work was to understand the municipal wastewater contribution to neonicotinoids in a representative, characterized effluent-dominated temperate-region stream. Our approach was to quantify the spatiotemporal...
Authors
Danielle T. Webb, Hui Zhi, Dana W. Kolpin, Rebecca D. Klaper, Luke R. Iwanowicz, Gregory H. LeFevre
Public and private tapwater: Comparative analysis of contaminant exposure and potential risk, Cape Cod, Massachusetts, USA Public and private tapwater: Comparative analysis of contaminant exposure and potential risk, Cape Cod, Massachusetts, USA
Background Humans are primary drivers of environmental contamination worldwide, including in drinking-water resources. In the United States (US), federal and state agencies regulate and monitor public-supply drinking water while private-supply monitoring is rare; the current lack of directly comparable information on contaminant-mixture exposures and risks between private- and public...
Authors
Paul M. Bradley, Denis R. LeBlanc, Kristin M. Romanok, Kelly Smalling, Michael J. Focazio, Mary C. Cardon, Jimmy Clark, Justin M. Conley, Nicola Evans, Carrie E. Givens, James L. Gray, L. Earl Gray, Phillip C. Hartig, Christopher P. Higgins, Michelle Hladik, Luke R. Iwanowicz, Keith A. Loftin, R. Blaine McCleskey, Carrie A. McDonough, Elizabeth Medlock-Kakaley, Christopher P. Weis, Vickie S. Wilson
By
Ecosystems Mission Area, Water Resources Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, California Water Science Center, Eastern Ecological Science Center, New England Water Science Center, New Jersey Water Science Center, South Atlantic Water Science Center (SAWSC), Upper Midwest Water Science Center, National Water Quality Laboratory
Genome sequences of 26 white sucker hepatitis B virus isolates from white sucker, catostomus commersonii, inhabiting transboundary waters from Alberta, Canada, to the Great Lakes, USA Genome sequences of 26 white sucker hepatitis B virus isolates from white sucker, catostomus commersonii, inhabiting transboundary waters from Alberta, Canada, to the Great Lakes, USA
We report 26 genomes of the white sucker hepatitis B virus (WSHBV) from the white sucker, Catostomus commersonii. Genome length ranged from 3541 to 3543 bp and nucleotide identity was 96.7% or greater across genomes. This work suggests a geographical range of this virus that minimally extends from the Athabasca River, Alberta, Canada to the Great Lakes, USA.
Authors
Cynthia R. Adams, Vicki S. Blazer, Jim Sherry, Robert S. Cornman, Luke R. Iwanowicz
Production and characterization of a mouse monoclonal antibody against smallmouth bass (Micropterus dolomieu) IgM Production and characterization of a mouse monoclonal antibody against smallmouth bass (Micropterus dolomieu) IgM
A murine monoclonal antibody (mAb, IgG2a) was produced for the detection of smallmouth bass (Micropterus dolomieu) immunoglobulin (IgM). The antibody is specific for IgM heavy chain and was shown to also recognize the Ig heavy chain of largemouth bass (Micropterus salmoides) and bluegill (Lepomis macrochirus) using Western Blot analysis of plasma from 9 teleost taxa. When applied to the...
Authors
Christopher Ottinger, Cheyenne R. Smith, Vicki S. Blazer, Luke R. Iwanowicz, Mary Ann Vogelbein, Stephen Kaattari
Non-USGS Publications**
Iwanowicz, L., L Brown, B Eltz, F Juanes and J Murt (2004) Book Review. In a Perfect Ocean: The state of fisheries and ecosystems in the North Atlantic Ocean. Reviews in Fish Biology and Fisheries.14: 149-151.
McIntire, M., L R Iwanowicz and A E Goodwin (2003) Molecular, physical and clinical evidence that Golden Shiner Virus (GSV) and Grass Carp Reovirus (GCRV) are variants of the same virus. Journal of Aquatic Animal Health. 15: 257-263.
Iwanowicz, L.R. and A E Goodwin (2002) Characterization of a new bacilliform fathead minnow rhabdovirus that produces syncytia in tissue culture. Archives of Virology 147: 889 – 916.
Iwanowicz, L.R. A E Goodwin and J Harshbarger (2001) Embryonal rhabdomyosarcoma of the giant gourami, Colisa fasciata (Bloch & Schneider). Journal of Fish Diseases 24:177-179
Iwanowicz, L.R. A E Goodwin and N Heil (2000) A small RNA virus isolated from apparently healthy wild sandbar shiners, Notropis scepticus. Journal of Fish Diseases 23: 349-352.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Science and Products
Filter Total Items: 27
Estrogen equivalents of surface water in Pennsylvania, 2012-2017 Estrogen equivalents of surface water in Pennsylvania, 2012-2017
The data were gathered as part of a long-term, seasonal assessment of estrogenicity in selected locations within Pennsylvania from 2012-2017. Data were collected over this time frame to determine seasonal and annual changes in surface water estrogenicity.Estrogen is reported as estrogen equivalents (EEQ) as determined by a bioluminescent yeast estrogen screen. A total of 193 grab water...
Data supporting: Draft Genome Sequence of a Novel Calicivirus from a Brown Bullhead (Ameiurus nebulosus) from Lake Memphremagog, VT Data supporting: Draft Genome Sequence of a Novel Calicivirus from a Brown Bullhead (Ameiurus nebulosus) from Lake Memphremagog, VT
We report a draft genome of a previously undescribed calicivirus from a single brown bullhead inhabiting Lake Memphremagog, Vermont/Quebec. The genome is 7413 nt in length and is most similar to the Atlantic salmon calicivirus (nucleotide identity: 64.7%).
Current use pesticides in larval amphibian tissues, amphibian pathogen and wetland sediment screening data from three northeastern National Wildlife Refuges, 2013-2014 Current use pesticides in larval amphibian tissues, amphibian pathogen and wetland sediment screening data from three northeastern National Wildlife Refuges, 2013-2014
The data include concentrations of current use pesticides in tissues of larval wood frog (Lithobates sylvaticus) and spotted salamander (Ambystoma maculatum) and the presence of ranavirus in wood frogs and spotted salamanders from three northeastern National Wildlife Refuges sampled in 2013 and 2014. The data also include estrogenicity, protein phosphatase 2A inhibition and a suite of 15...
Water-quality data for a pharmaceutical study at Muddy Creek in North Liberty and Coralville, Iowa, 2017-2018 Water-quality data for a pharmaceutical study at Muddy Creek in North Liberty and Coralville, Iowa, 2017-2018
Samples were collected, processed, and analyzed for organics, estrogen equivalents, and fecal indicator bacteria. Filtered organic samples were sent to the National Water Quality Laboratory in Denver, Colorado. Unfiltered estrogen equivalent samples were sent to the Organic Geochemistry Research Lab in Lawrence, Kansas, for extraction, after which they were sent to the National Fish...
2004-2010 Chesapeake Bay Smallmouth and Largemouth Bass Estrogenic Biomarker Data 2004-2010 Chesapeake Bay Smallmouth and Largemouth Bass Estrogenic Biomarker Data
The data was gathered to document the extent and severity of biomarkers of exposure to estrogenic chemicals in smallmouth and largemouth bass. Samples were collected from 2004 through 2010 at sites in five watersheds within the Chesapeake Bay watershed. A total of 281 largemouth bass were collected from the Potomac, Susquehanna, Patuxent and Rappahannock rivers and 1,384 smallmouth bass...
Estrogen equivalents of surface water and smallmouth bass estrogenic biomarker data in New Jersey, 2016-2017 Estrogen equivalents of surface water and smallmouth bass estrogenic biomarker data in New Jersey, 2016-2017
The data were gathered as a preliminary assessment of estrogenicity under base-flow conditions at over 100 sites (lakes and streams) throughout New Jersey followed by more targeted sampling of smallmouth bass at nine sites with varying levels of estrogenicity. In 2016, 102 sites (lakes and streams) for the preliminary estrogenicity assessment were selected utilizing compiled results of...
Microbial and chemical contaminant occurrence and concentration in groundwater and surface water proximal to large-scale poultry facilities and poultry litter, 2016 Microbial and chemical contaminant occurrence and concentration in groundwater and surface water proximal to large-scale poultry facilities and poultry litter, 2016
Chemical and biological results, quality assurance and quality control, and method information from groundwater, surface water, and litter samples, collected from nine locations in Iowa and one in Wisconsin in 2016. Thirteen groundwater, nine surface water, four poultry litter, and four QA/QC samples were collected. Samples were analyzed at U.S. Geological Survey laboratories; bacteria...
Toxicity of potassium chloride, active compound in the molluscicide potash, on salmonid fishes and their forage base (Leetown Science Center, 2018). Toxicity of potassium chloride, active compound in the molluscicide potash, on salmonid fishes and their forage base (Leetown Science Center, 2018).
The data is supplemental to the 2018 USGS Open File Report, "An evaluation of the acute and chronic toxicity of potassium chloride, active compound in the molluscicide potash, on salmonid fishes and their forage base". This research was performed as a Science Support Partnership (SSP) study with USFWS in order to evaluate potential lethal and sublethal impacts of potassium chloride on...
Molecular detection of avian influenza virus from sediment samples in waterfowl habitats on the Delmarva Peninsula, USA Molecular detection of avian influenza virus from sediment samples in waterfowl habitats on the Delmarva Peninsula, USA
Data were collected as part of an investigation developed by Leetown Science Center to investigate the comparative detection of avian influenza viruses from waterfowl and potential environmental reservoirs such as aquatic sediment from waterfowl habitat. This dataset identifies positive or negative test results for qRT-PCR (quantitative reverse transcriptase polymerase chain reaction)...
Estrogen Equivalents of Surface Water in the Upper Chesapeake Bay Watershed (2008 - 2011) Estrogen Equivalents of Surface Water in the Upper Chesapeake Bay Watershed (2008 - 2011)
Intersex in small- and largemouth bass has been observed in the upper Chesapeake Bay Watershed since 2003. This observation is indicative of exposure to estrogenic endocrine disrupting chemicals. In order to complement biological data sets and better identify the spatial and temporal nature of this end-point, discrete grab water samples were collected throughout the upper Chesapeake Bay...
Chelonid Herpesvirus 5 (ChHV5) genomic sequences obtained through long-range PCR and high throughput sequencing from Florida and Hawaii green sea turtles with fibropapillomatosis Chelonid Herpesvirus 5 (ChHV5) genomic sequences obtained through long-range PCR and high throughput sequencing from Florida and Hawaii green sea turtles with fibropapillomatosis
The files in this data release are the processed DNA consensus sequence alignment files for the chelonid herpesvirus 5 (ChHV-5) genome plus 66 open reading frames representing protien-coding genes. DNA was extracted from tumor biopsies from green sea turtles (Chelonias mydas) with fibropapillomatosis sampled from Florida and Hawaii coastal waters. We used high-throughput short-read...
Analytical inorganic chemistry results for samples collected for the Sediment-Bound Contaminant Resiliency and Response Strategy pilot study, northeastern United States, 2015 Analytical inorganic chemistry results for samples collected for the Sediment-Bound Contaminant Resiliency and Response Strategy pilot study, northeastern United States, 2015
Coastal communities are uniquely vulnerable to sea-level rise (SLR) and severe storms such as hurricanes. These events enhance the dispersion and concentration of natural and anthropogenic chemicals and pathogenic microorganisms that could adversely affect the health and resilience of coastal communities and ecosystems in coming years. The U.S. Geological Survey has developed the...
Filter Total Items: 110
Transcriptome signatures of wastewater effluent exposure in larval zebrafish vary with seasonal mixture composition in an effluent-dominated stream Transcriptome signatures of wastewater effluent exposure in larval zebrafish vary with seasonal mixture composition in an effluent-dominated stream
Wastewater treatment plant (WWTP) effluent-dominated streams provide critical habitat for aquatic and terrestrial organisms but also continually expose them to complex mixtures of pharmaceuticals that can potentially impair growth, behavior, and reproduction. Currently, few biomarkers are available that relate to pharmaceutical-specific mechanisms of action. In the experiment reported in...
Authors
Emma B. Meade, Luke R. Iwanowicz, Nicklaus Neureuther, Gregory H. LeFevre, Dana W. Kolpin, Hui Zhi, Shannon M. Meppelink, Rachael F. Lane, Angela Schmoldt, Aurash Mohaimani, Olaf Mueller, Rebecca D. Klaper
Using biological responses to monitor freshwater post-spill conditions over 3 years in Blacktail Creek, North Dakota, USA Using biological responses to monitor freshwater post-spill conditions over 3 years in Blacktail Creek, North Dakota, USA
A pipeline carrying unconventional oil and gas (OG) wastewater spilled approximately 11 million liters of wastewater into Blacktail Creek, North Dakota, USA. Flow of the mix of stream water and wastewater down the channel resulted in storage of contaminants in the hyporheic zone and along the banks, providing a long-term source of wastewater constituents to the stream. A multi-level...
Authors
Aida Farag, David Harper, Isabelle M. Cozzarelli, Douglas B. Kent, Adam Mumford, Denise M. Akob, Travis W. Schaeffer, Luke R. Iwanowicz
By
Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, Columbia Environmental Research Center, Earth Resources Observation and Science (EROS) Center , Eastern Ecological Science Center, Geology, Energy & Minerals Science Center, Reston Microbiology Laboratory
RNA-seq reveals potential gene biomarkers in fathead minnows (Pimephales promelas) for exposure to treated wastewater effluent RNA-seq reveals potential gene biomarkers in fathead minnows (Pimephales promelas) for exposure to treated wastewater effluent
Discharged wastewater treatment plant (WWTP) effluent greatly contributes to the generation of complex mixtures of contaminants of emerging concern (CECs) in aquatic environments which often contain neuropharmaceuticals and other emerging contaminants that may impact neurological function. However, there is a paucity of knowledge on the neurological impacts of these exposures to aquatic...
Authors
Peter Schumann, E. Meade, H. Zhi, G. H. LeFevre, Dana W. Kolpin, Shannon M. Meppelink, Luke R. Iwanowicz, Rachael F. Lane, A. Schmoldt, O. Mueller, R. D. Klaper
Freshwater unionid mussels threatened by predation of Round Goby (Neogobius melanostomus) Freshwater unionid mussels threatened by predation of Round Goby (Neogobius melanostomus)
Indigenous freshwater mussels (Unionidae) are integral to riverine ecosystems, playing a pivotal role in aquatic food webs and providing ecological services. With populations on the decline worldwide, freshwater mussels are of conservation concern. In this study, we explore the propensity of the invasive Round Goby (Neogobius melanostomus) fish to prey upon indigenous freshwater mussels...
Authors
Kyle Clark, Deborah D. Iwanowicz, Luke R. Iwanowicz, Sara Mueller, Joshua Wisor, Casey Bradshaw-Wilson, W. Bane Schill, Jay R. Stauffer, Elizabeth W. Boyer
Draft genome sequence of a novel calicivirus from a brown bullhead (Ameiurus nebulosus) from Lake Memphremagog, Vermont/Quebec Draft genome sequence of a novel calicivirus from a brown bullhead (Ameiurus nebulosus) from Lake Memphremagog, Vermont/Quebec
We report a draft genome sequence of a previously undescribed calicivirus from a single brown bullhead inhabiting Lake Memphremagog, Vermont/Quebec. The genome is 7,413 nucleotides long and is most similar to the Atlantic salmon calicivirus (nucleotide identity; 64.7%).
Authors
Luke R. Iwanowicz, Vicki S. Blazer, Tom Jones, Matthew Bodnar, Korin Ekholm, Julie Dragon, Peter Emmerson
Site- and individual-level contaminations affect infection prevalence of an emerging infectious disease of amphibians Site- and individual-level contaminations affect infection prevalence of an emerging infectious disease of amphibians
Emerging infectious disease outbreaks are one of multiple stressors responsible for amphibian declines globally. In the northeastern United States, ranaviral diseases are prevalent in amphibians and other ectothermic species, but there is still uncertainty as to whether their presence is leading to population level effects. Further, there is also uncertainty surrounding the potential...
Authors
Kelly L. Smalling, Brittany A. Mosher, Luke R. Iwanowicz, Keith A. Loftin, Adam Boehlke, Michelle L. Hladik, Carly R. Muletz-Wolz, Nandadevi Cortes-Rodriguez, Robin Femmer, Evan H. Campbell Grant
By
Ecosystems Mission Area, Water Resources Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, Central Plains Water Science Center, Eastern Ecological Science Center, California Water Science Center, Geology, Geophysics, and Geochemistry Science Center, New Jersey Water Science Center
Tandem field and laboratory approaches to quantify attenuation mechanisms of pharmaceutical and pharmaceutical transformation products in a wastewater effluent-dominated stream Tandem field and laboratory approaches to quantify attenuation mechanisms of pharmaceutical and pharmaceutical transformation products in a wastewater effluent-dominated stream
Evolving complex mixtures of pharmaceuticals and transformation products in effluent-dominated streams pose potential impacts to aquatic species; thus, understanding the attenuation dynamics in the field and characterizing the prominent attenuation mechanisms of pharmaceuticals and their transformation products (TPs) is critical for hazard assessments. Herein, we determined the...
Authors
Hui Zhi, Alyssa L Mianecki, Dana W. Kolpin, Rebecca D. Klaper, Luke R. Iwanowicz, Gregory H. LeFevre
Pilot-scale expanded assessment of inorganic and organic tapwater exposures and predicted effects in Puerto Rico, USA Pilot-scale expanded assessment of inorganic and organic tapwater exposures and predicted effects in Puerto Rico, USA
A pilot-scale expanded target assessment of mixtures of inorganic and organic contaminants in point-of-consumption drinking water (tapwater, TW) was conducted in Puerto Rico (PR) to continue to inform TW exposures and corresponding estimations of cumulative human-health risks across the US. In August 2018, a spatial synoptic pilot assessment of than 524 organic, 37 inorganic, and select
Authors
Paul M. Bradley, Ingrid Y. Padilla, Kristin M. Romanok, Kelly Smalling, Michael J. Focazio, Sara E. Breitmeyer, Mary C. Cardon, Justin M. Conley, Nicola Evans, Carrie E. Givens, James L. Gray, L. Earl Gray, Phillip C. Hartig, Michelle L. Hladik, Christopher P. Higgins, Luke R. Iwanowicz, Rachael F. Lane, Keith A. Loftin, R. Blaine McCleskey, Carrie A. McDonough, Elizabeth Medlock-Kakaley, Shannon M. Meppelink, Christopher P. Weis, Vickie S. Wilson
Emerging investigator series: Municipal wastewater as a year-round point source of neonicotinoid insecticides that persist in an effluent-dominated stream Emerging investigator series: Municipal wastewater as a year-round point source of neonicotinoid insecticides that persist in an effluent-dominated stream
Neonicotinoids in aquatic systems have been predominantly associated with agriculture, but some are increasingly being linked to municipal wastewater. Thus, the aim of this work was to understand the municipal wastewater contribution to neonicotinoids in a representative, characterized effluent-dominated temperate-region stream. Our approach was to quantify the spatiotemporal...
Authors
Danielle T. Webb, Hui Zhi, Dana W. Kolpin, Rebecca D. Klaper, Luke R. Iwanowicz, Gregory H. LeFevre
Public and private tapwater: Comparative analysis of contaminant exposure and potential risk, Cape Cod, Massachusetts, USA Public and private tapwater: Comparative analysis of contaminant exposure and potential risk, Cape Cod, Massachusetts, USA
Background Humans are primary drivers of environmental contamination worldwide, including in drinking-water resources. In the United States (US), federal and state agencies regulate and monitor public-supply drinking water while private-supply monitoring is rare; the current lack of directly comparable information on contaminant-mixture exposures and risks between private- and public...
Authors
Paul M. Bradley, Denis R. LeBlanc, Kristin M. Romanok, Kelly Smalling, Michael J. Focazio, Mary C. Cardon, Jimmy Clark, Justin M. Conley, Nicola Evans, Carrie E. Givens, James L. Gray, L. Earl Gray, Phillip C. Hartig, Christopher P. Higgins, Michelle Hladik, Luke R. Iwanowicz, Keith A. Loftin, R. Blaine McCleskey, Carrie A. McDonough, Elizabeth Medlock-Kakaley, Christopher P. Weis, Vickie S. Wilson
By
Ecosystems Mission Area, Water Resources Mission Area, Contaminant Biology, Environmental Health Program, Toxic Substances Hydrology, California Water Science Center, Eastern Ecological Science Center, New England Water Science Center, New Jersey Water Science Center, South Atlantic Water Science Center (SAWSC), Upper Midwest Water Science Center, National Water Quality Laboratory
Genome sequences of 26 white sucker hepatitis B virus isolates from white sucker, catostomus commersonii, inhabiting transboundary waters from Alberta, Canada, to the Great Lakes, USA Genome sequences of 26 white sucker hepatitis B virus isolates from white sucker, catostomus commersonii, inhabiting transboundary waters from Alberta, Canada, to the Great Lakes, USA
We report 26 genomes of the white sucker hepatitis B virus (WSHBV) from the white sucker, Catostomus commersonii. Genome length ranged from 3541 to 3543 bp and nucleotide identity was 96.7% or greater across genomes. This work suggests a geographical range of this virus that minimally extends from the Athabasca River, Alberta, Canada to the Great Lakes, USA.
Authors
Cynthia R. Adams, Vicki S. Blazer, Jim Sherry, Robert S. Cornman, Luke R. Iwanowicz
Production and characterization of a mouse monoclonal antibody against smallmouth bass (Micropterus dolomieu) IgM Production and characterization of a mouse monoclonal antibody against smallmouth bass (Micropterus dolomieu) IgM
A murine monoclonal antibody (mAb, IgG2a) was produced for the detection of smallmouth bass (Micropterus dolomieu) immunoglobulin (IgM). The antibody is specific for IgM heavy chain and was shown to also recognize the Ig heavy chain of largemouth bass (Micropterus salmoides) and bluegill (Lepomis macrochirus) using Western Blot analysis of plasma from 9 teleost taxa. When applied to the...
Authors
Christopher Ottinger, Cheyenne R. Smith, Vicki S. Blazer, Luke R. Iwanowicz, Mary Ann Vogelbein, Stephen Kaattari
Non-USGS Publications**
Iwanowicz, L., L Brown, B Eltz, F Juanes and J Murt (2004) Book Review. In a Perfect Ocean: The state of fisheries and ecosystems in the North Atlantic Ocean. Reviews in Fish Biology and Fisheries.14: 149-151.
McIntire, M., L R Iwanowicz and A E Goodwin (2003) Molecular, physical and clinical evidence that Golden Shiner Virus (GSV) and Grass Carp Reovirus (GCRV) are variants of the same virus. Journal of Aquatic Animal Health. 15: 257-263.
Iwanowicz, L.R. and A E Goodwin (2002) Characterization of a new bacilliform fathead minnow rhabdovirus that produces syncytia in tissue culture. Archives of Virology 147: 889 – 916.
Iwanowicz, L.R. A E Goodwin and J Harshbarger (2001) Embryonal rhabdomyosarcoma of the giant gourami, Colisa fasciata (Bloch & Schneider). Journal of Fish Diseases 24:177-179
Iwanowicz, L.R. A E Goodwin and N Heil (2000) A small RNA virus isolated from apparently healthy wild sandbar shiners, Notropis scepticus. Journal of Fish Diseases 23: 349-352.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government