I am an environmental organic chemist whose current research focuses on the fate and transport of current-use pesticides and other organic contaminants in aquatic and terrestrial environments.
Much of my research is focused on pesticides but also includes other organic contaminants. I direct a research laboratory that is focused on developing new methods to measure pesticides and their degradates in water, sediment, and biota. My laboratory specializes in small samples sizes, complex matrices, and non-standard procedues. Additionally, I also work on developing methods and measuring disinfection by-products in treated water (especially those that are not-currently regulated and are understudied) with a focus on wastewater discharges.
Professional Experience
Research Chemist, USGS, California Water Science Center, Sacramento, 2006 to present
Chemist, USGS, California Water Science Center, Sacramento, 2005-2006
Graduate Fellow/Research and Teaching Assistant, Deparment of Geography and Environmental Engineering, Johns Hopkins University, 1999-2005
Undergraduate Research Summer Institute, Department of Chemistry, Vassar College, 1997,1998
Undergraduate Research and Teaching Assistant, Department of Chemistry, Vassar College ,1997,1998
Education and Certifications
Ph.D. 2005, Environmental Engineering and Chemistry, Johns Hopkins University, Baltimore, Maryland
B.A. 1999, Chemistry, Vassar College, Poughkeepsie, New York
Science and Products
A National Assessment of Pesticide, PFAS, Microplastic, and Antibiotic Resistance Gene Exposures in White-Tailed Deer
Collaborative Science Provides Understanding of Contaminants in Bottled Water-an Increasingly Common Alternate Drinking Water Source
Wild Prairie Grouse Diet and Microbiomes Vary Between Cropland and Grassland Habitats
Pesticides Detected in Bees, Flowers, Soil, and Air within Pollinator-Attractive Row-Crop Border Plantings
Science to Understand Low-Level Exposures to Neonicotinoid Pesticides, their Metabolites, and Chlorinated Byproducts in Drinking Water
Clothianidin Exposure Associated with Changes in Tadpole Behavior
Costs and Benefits of Nitrapyrin
Dysbiosis, immunomodulation, and health effects of agricultural pesticides in wild prairie grouse
Environmental Management of Vector-borne Diseases
Water-Quality Inventory Pesticides in the Golden Gate National Recreation Area
Organic Chemistry Research — Sacramento, California
Pesticide Fate Research Group (PFRG)
Pesticide Concentrations Measured in Zooplankton Samples Collected from the Sacramento River and Yolo Bypass, California, 2021
Target-chemical concentrations and bioassay results for assessment of mixed-organic/inorganic chemical and biological exposures in Minneapolis/St Paul, Minnesota tapwater, August 2019
Results from U.S. Geological Survey Environmental Health Food Resources Lifecycle Integrated Science Team, Graton Pesticides (GRAPE) Study, in Graton, California, April–May 2021
Multiresidue extraction of current-use pesticides from complex solid matrices using energized dispersive extraction with analysis by gas and liquid chromatography tandem mass spectroscopy
Pesticide Concentrations in American Bullfrog (Lithobates catesbeianus) Tadpoles Collected from Ponds and Reservoirs near Mead, Nebraska
Target-Chemical Concentrations and Microbiological Results for Assessment of Mixed Contaminant and Biological Exposures in Bottled Water, 2020
Pesticide residues in passive samplers and bee tissue from Conservation Reserve Program fields across an agricultural gradient in eastern Iowa, USA, 2019
Pesticide concentrations in bees and other matrices collected from sunflower fields (with and without a neonicotinoid seed treatment) near Sacramento, California
Naled and dichlorvos in water and aquatic organisms from a canal and rice fields near Sacramento, California
Current-use pesticides, nutrients, chloride, and metals in ponds and stormwater basins of the New Jersey Pinelands, 2018-20
Water-quality results from a wastewater reuse study: Inorganic and organic compositions of wastewater effluent and select urban and agricultural water types during rain-induced runoff, Chickasha, Oklahoma, 2018-2019
Characterizing microbiota, virulome, and resistome of wild prairie grouse in crop producing and uncultivated areas of Nebraska
Bifenthrin, a ubiquitous contaminant, impairs the development and behavior of the threatened Longfin Smelt during early life stages
Neonicotinoid sunflower seed treatment, while not detected in pollen and nectar, still impacts wild bees and crop yield
Comparative toxicity of two neonicotinoid insecticides at environmentally relevant concentrations to telecoprid dung beetles
Exposures and potential health implications of contaminant mixtures in linked source water, finished drinking water, and tapwater from public-supply drinking water systems in Minneapolis/St. Paul area, USA
Multiresidue extraction of current-use pesticides from complex solid matrices using energized dispersive guided extraction with analysis by gas and liquid chromatography tandem mass spectroscopy
Field assessment of Naled and its primary degradation product (dichlorvos) in aquatic ecosystems following aerial ultra-low volume application for mosquito control
Assessing the presence of current-use pesticides in mid-elevation Sierra Nevada streams using passive samplers, California, 2018–19
Juxtaposition of intensive agriculture, vulnerable aquifers, and mixed chemical/microbial exposures in private-well tapwater in northeast Iowa
Contaminant exposure and transport from three potential reuse waters within a single watershed
Wild bee exposure to pesticides in conservation grasslands increases along an agricultural gradient: A tale of two sample types
Pesticide prioritization by potential biological effects in tributaries of the Laurentian Great Lakes
Bottled water contaminant exposures and potential human effects
Science and Products
- Science
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A National Assessment of Pesticide, PFAS, Microplastic, and Antibiotic Resistance Gene Exposures in White-Tailed Deer
Research has documented exposures and consequential environmental health effects of pesticides, PFAS, microplastics, and antibiotic resistance genes in environmental biota. Little is known, however, regarding such effects in white-tailed deer (Odocoileus virginianus).Collaborative Science Provides Understanding of Contaminants in Bottled Water-an Increasingly Common Alternate Drinking Water Source
U.S. Geological Survey researchers and public health experts collaborated to determine what contaminants occur in bottled water, which is an increasingly common alternate drinking water source, to broaden their understanding of human exposure to contaminants in drinking water supply chains. Bottled water, like public-supply and private-well tap water supply chains, contained multiple organic...Wild Prairie Grouse Diet and Microbiomes Vary Between Cropland and Grassland Habitats
Wild prairie grouse residing in croplands had altered diets and gut microbiome imbalances characterized by a greater abundance of pathogenic bacteria and antibiotic-resistance genes in comparison to those residing in grasslands. Similar gut microbiome imbalances are rarely associated with lethal outcomes, but rather linked to sublethal health effects including growth, development, behavior, immune...Pesticides Detected in Bees, Flowers, Soil, and Air within Pollinator-Attractive Row-Crop Border Plantings
Field study in California describes the potential for pollinator-attractive field borders in agricultural areas to become a pesticide exposure pathway to bees through soil, air, and plants.Science to Understand Low-Level Exposures to Neonicotinoid Pesticides, their Metabolites, and Chlorinated Byproducts in Drinking Water
Scientists reported the discovery of three neonicotinoid pesticides in drinking water and their potential for transformation and removal during water treatment. The research provides new insights into the persistence of neonicotinoids and their potential for transformation during water treatment and distribution, while also identifying granulated activated carbon as a potentially effective...Clothianidin Exposure Associated with Changes in Tadpole Behavior
During a laboratory exposure study, tadpole movement decreased with increased concentrations of clothianidin, a neonicotinoid pesticide. Decreased movement could affect a tadpole’s ability to forage, escape predation, and metamorphose before ponds dry.Costs and Benefits of Nitrapyrin
In December 2020, our research group (as part of the Food Integrated Science Team) published a review of nitrapyrin costs and benefits in the journal Environmental Science & Technology.Dysbiosis, immunomodulation, and health effects of agricultural pesticides in wild prairie grouse
Agrochemical pollution poses a severe threat to biodiversity. Agrochemicals can detrimentally affect wildlife growth, development, survival, reproduction, and immune responses, which facilitates emergence and spread of infectious diseases that may cause unusually high mortality. Animal microbiota plays a fundamental role in host’s food detoxification and defense against pathogens, regulates...Environmental Management of Vector-borne Diseases
Effective management programs for vector-borne pathogens, such as West Nile Virus and the Lyme disease spirochete, are necessary to protect public health. However, some vector control methods, such as landscape manipulations and pesticide applications, can also adversely affect nontarget species and environmentally sensitive natural systems. Efficient targeting and integration of vector control...Water-Quality Inventory Pesticides in the Golden Gate National Recreation Area
In 1998, all urban creeks in the San Francisco Bay Area were added to the Clean Water Act Section 303d list due to known or suspected diazinon impairment. Diazinon is an organophosphorus insecticide used on lawns, and fruit and vegetables. This pesticide has since been "phased out" and has not been available for purchase since late 2004. Diazinon use is declining and is being replaced by other...Organic Chemistry Research — Sacramento, California
About the ResearchThe Environmental Health Program collaborates with chemists and hydrologists at the Organic Chemistry Research Laboratory (OCRL) to develop targeted analytical methods for the quantitation of chemicals that can impact the health of organisms and humans. The scientists have developed methods in a wide variety of environmental media; in addition to water and sediment, they also...Pesticide Fate Research Group (PFRG)
Pesticides are applied in agricultural and urban areas to control weeds, insects, fungus, and other pests. Applied pesticides and their degradates can be transported off-site through a variety of mechanisms; these pesticides can then be found in non-target areas. Pesticide transport can occur through the atmosphere, in the aqueous phase (surface and groundwater) and associated with soil/sediment... - Data
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Pesticide Concentrations Measured in Zooplankton Samples Collected from the Sacramento River and Yolo Bypass, California, 2021
Zooplankton samples were collected at one site upstream of the Yolo Bypass in Northern California, three sites within the Bypass, and at a comparison site on the Sacramento River below the city of Sacramento and analyzed for a large suite of current-use pesticides and degradates. Samples were collected every two weeks from early August 2021 to mid-October 2021. Samples were collected by towing a 1Target-chemical concentrations and bioassay results for assessment of mixed-organic/inorganic chemical and biological exposures in Minneapolis/St Paul, Minnesota tapwater, August 2019
This dataset contains the concentration and quality assurance results for inorganic and organic analytes collected for the U.S. Geological Survey (USGS), Ecosystems Mission Area, Environmental Health Infrastructure Program, Tapwater Exposure Project conducted in Minnesota, in August of 2019. Samples were obtained from multiple publicly distributed water sources in Minnesota in 2019. Due to privacyResults from U.S. Geological Survey Environmental Health Food Resources Lifecycle Integrated Science Team, Graton Pesticides (GRAPE) Study, in Graton, California, April–May 2021
In the spring of 2021, six households with private wells were selected throughout Graton, California, based on proximity to agriculture (within one mile of agriculture), well depth (i.e., less than 150 feet in depth), and well type (i.e., dug versus drilled). Silicone bands were deployed for 30 days at outdoor locations at each household to passively sample pesticides in ambient air. On May 3, 202Multiresidue extraction of current-use pesticides from complex solid matrices using energized dispersive extraction with analysis by gas and liquid chromatography tandem mass spectroscopy
A multiresidue method for solids (i.e., soil, sediment, sludge) was developed and evaluated for the analysis of pesticides and pesticide degradates. Initially, a well characterized soil was used to determine the recovery of target compounds using an Energized Dispersive Guided Extraction (EDGE) system with two extraction solvents, acetonitrile and 1:1 (v/v) hexane:acetone. Extracts were cleaned usPesticide Concentrations in American Bullfrog (Lithobates catesbeianus) Tadpoles Collected from Ponds and Reservoirs near Mead, Nebraska
To evaluate the potential uptake and accumulation of pesticides in amphibians, American bullfrog (Lithobates catesbeianus) tadpoles were collected from surface water sites (four ponds and one reservoir) in 2021 near Mead, Nebraska. Sites were located in the landscape surrounding the AltEn ethanol plant, a plant which had previously received pesticide treated (coated) seeds. Tadpoles were collectedTarget-Chemical Concentrations and Microbiological Results for Assessment of Mixed Contaminant and Biological Exposures in Bottled Water, 2020
This data release contains concentration and quality-assurance results for inorganic, organic analytes and microbiological pathogens collected from 30 different commercial bottled water sources. Samples were processed on July 20 and August 17, 2020 at the New Jersey Water Science Center and analyzed at various U.S. Geological Survey laboratories. Samples were analyzed for nutrients, cations and anPesticide residues in passive samplers and bee tissue from Conservation Reserve Program fields across an agricultural gradient in eastern Iowa, USA, 2019
This data release includes sampling location, pesticide concentrations in passive samplers (silicone bands) and bees foraging in Conservation Reserve Program fields. Sampling took place during July and August of 2019. Fields were located on private land managed for the U.S. Department of Agriculture Conservation Reserve Program in eastern central Iowa, U.S.A.Pesticide concentrations in bees and other matrices collected from sunflower fields (with and without a neonicotinoid seed treatment) near Sacramento, California
Hybrid sunflower production occurs near Sacramento in Northern California where seed treatments are commonly used. In this study, four locations were sampled in 2016 as matched pairs; half of each field was sown with thiamethoxam treated seed and half without. In addition to the thiamethoxam seed treatment, all seeds were coated with two fungicides, fludioxonil and mefenoxam/metalaxyl. Both wild bNaled and dichlorvos in water and aquatic organisms from a canal and rice fields near Sacramento, California
Ultra-low volumes of naled are applied by aircraft over rice fields and canals to reduce adult mosquito populations near Sacramento, California. Each summer, the pesticide is applied approximately 7-10 times between July and October. Naled and its major degradate (dichlorvos) were quantified in water, biofilm, and invertebrates following aerial applications. In 2020, samples were collected beforeCurrent-use pesticides, nutrients, chloride, and metals in ponds and stormwater basins of the New Jersey Pinelands, 2018-20
The data presented in this data release include concentrations of current-use pesticides, nutrients, chloride, and metals in surface water collected from 60 wetlands including natural ponds, excavated ponds (Xponds), and stormwater basins in the New Jersey Pinelands, 2018-20. Site information, basic water-quality parameters, and method information also are included.Water-quality results from a wastewater reuse study: Inorganic and organic compositions of wastewater effluent and select urban and agricultural water types during rain-induced runoff, Chickasha, Oklahoma, 2018-2019
Oklahoma State University South-Central Research Station (SCRS) was used to conduct research to understand the chemical composition of various water types and their potential environmental and human health effects. The study area provided the opportunity to study five water types: (1) receiving surface water (Washita River), (2) urban stormwater, (3) wastewater treatment plant effluent used for irCharacterizing microbiota, virulome, and resistome of wild prairie grouse in crop producing and uncultivated areas of Nebraska
Chart listing the geographic locations, NCBI numbers, voucher numbers, and microbiome characteristics of specimens collected for publication - Multimedia
- Publications
Filter Total Items: 103
Bifenthrin, a ubiquitous contaminant, impairs the development and behavior of the threatened Longfin Smelt during early life stages
The Longfin Smelt (Spirinchus thaleichthys) population in the San Franscisco Bay/Sacramento-San Joaquin Delta (Bay-Delta) has declined to ∼1% of its pre-1980s abundance and, as a result, is listed as threatened under the California Endangered Species Act. The reasons for this decline are multiple and complex, including the impacts of contaminants. Because the spawning and rearing seasons of LongfiAuthorsFlorian Mauduit, Amelie Segarra, Julia Sherman, Michelle Hladik, Luann Wong, Thomas M Young, Levi Lewis, Tien-Chieh Hung, Nann A. Fangue, Richard E ConnonNeonicotinoid sunflower seed treatment, while not detected in pollen and nectar, still impacts wild bees and crop yield
Neonicotinoid seed treatments are commonly used in agricultural production even though their benefit to crop yield and their impact on pollinators, particularly wild bees, remains unclear. Using an on-farm matched pair design in which half of each field was sown with thiamethoxam treated seed and half without, we assessed honey bee and wild bee exposure to pesticides in sunflower fields by analyziAuthorsLaura T. Ward, Michelle Hladik, Aidee Guzman, Ariana Bautista, Nicholas MillsComparative toxicity of two neonicotinoid insecticides at environmentally relevant concentrations to telecoprid dung beetles
Dung beetles (Coleoptera: Scarabaeinae) frequently traverse agricultural matrices in search of ephemeral dung resources and spend extended periods of time burrowing in soil. Neonicotinoids are among the most heavily applied and widely detected insecticides used in conventional agriculture with formulated products designed for row crop and livestock pest suppression. Here, we determined the comparaAuthorsMichael C. Cavallaro, Michelle Hladik, Samantha Hittson, Greg Middleton, W. Wyatt HobackExposures and potential health implications of contaminant mixtures in linked source water, finished drinking water, and tapwater from public-supply drinking water systems in Minneapolis/St. Paul area, USA
Continued improvements in drinking-water quality characterization and treatment/distribution infrastructure are required to address the expanding number of documented environmental contaminants. To better understand the variability in contaminant exposures from the drinking water resource (surface and groundwater), through the distribution process, to the point-of-use (tapwater), in 2019 a synoptiAuthorsKelly Smalling, Paul Bradley, Kristin Romanok, Sarah M. Elliott, Jane de Lambert, Michael J. Focazio, Stephanie Gordon, James L. Gray, Leslie K. Kanagy, Michelle Hladik, Keith Loftin, R. Blaine McCleskey, Elizabeth Medlock-Kakaley, Mary C. Cardon, Nicola Evans, Christopher P. WeisMultiresidue extraction of current-use pesticides from complex solid matrices using energized dispersive guided extraction with analysis by gas and liquid chromatography tandem mass spectroscopy
The development of sample processing techniques that recover a broad suite of pesticides from solid matrices, while mitigating coextracted matrix interferences, and reducing processing time is beneficial for high throughput analyses. The objective of this study was to evaluate the effectiveness of an automated extraction system for pesticide analyses in solid environmental samples. An Energized DiAuthorsGabrielle Pecora Black, Emily Woodward, Corey Sanders, Michael S. Gross, Michelle HladikField assessment of Naled and its primary degradation product (dichlorvos) in aquatic ecosystems following aerial ultra-low volume application for mosquito control
Naled, an organophosphate insecticide, is applied aerially at ultra-low volumes over aquatic ecosystems near Sacramento, California, USA, during summer months for mosquito control. Two ecosystem types (rice fields and a flowing canal) were sampled in 2020 and 2021. Naled and its primary degradation product (dichlorvos) were measured in water, biofilm, grazer macroinvertebrates, and omnivore/predatAuthorsCassandra Smith, Michelle Hladik, Kathryn Kuivila, Ian R. WaiteAssessing the presence of current-use pesticides in mid-elevation Sierra Nevada streams using passive samplers, California, 2018–19
Passive sampler devices were deployed in six northern California streams five times between November 2018 and December 2019 to measure the presence or absence of current-use pesticides in surface water. In the targeted areas, there are reported pesticide uses for agriculture, commercial forestry, and rights of way maintenance along with unreported pesticide use at private residences and cannabis gAuthorsMatthew D. De Parsia, James L. Orlando, Michelle L. HladikJuxtaposition of intensive agriculture, vulnerable aquifers, and mixed chemical/microbial exposures in private-well tapwater in northeast Iowa
In the United States and globally, contaminant exposure in unregulated private-well point-of-use tapwater (TW) is a recognized public-health data gap and an obstacle to both risk-management and homeowner decision making. To help address the lack of data on broad contaminant exposures in private-well TW from hydrologically-vulnerable (alluvial, karst) aquifers in agriculturally-intensive landscapesAuthorsPaul Bradley, Dana W. Kolpin, Darrin A. Thompson, Kristin Romanok, Kelly Smalling, Sara Breitmeyer, Mary C. Cardon, David M. Cwiertny, Nicola Evans, R. William Field, Michael J. Focazio, Laura E. Beane Freeman, Carrie E Givens, James L. Gray, Gordon L. Hager, Michelle Hladik, Jonathan N. Hoffman, Rena R. Jones, Leslie K. Kanagy, Rachael F. Lane, R. Blaine McCleskey, Danielle Medgyesi, Elizabeth Medlock-Kakaley, Shannon M. Meppelink, Michael T. Meyer, Diana A. Stavreva, Mary H. WardContaminant exposure and transport from three potential reuse waters within a single watershed
Global demand for safe and sustainable water supplies necessitates a better understanding of contaminant exposures in potential reuse waters. In this study, we compared exposures and load contributions to surface water from the discharge of three reuse waters (wastewater effluent, urban stormwater, and agricultural runoff). Results document substantial and varying organic-chemical contribution toAuthorsJason R. Masoner, Dana W. Kolpin, Isabelle M. Cozzarelli, Paul Bradley, Brian Arnall, Kenneth J. Forshay, James L. Gray, Justin F. Groves, Michelle Hladik, Laura E. Hubbard, Luke R. Iwanowicz, Jeanne B. Jaeschke, Rachael F. Lane, R. Blaine McCleskey, Bridgette F. Polite, David A. Roth, Michael Pettijohn, Michaelah C. WilsonByWater Resources Mission Area, Environmental Health Program, Eastern Ecological Science Center, Colorado Water Science Center, California Water Science Center, Central Midwest Water Science Center, Geology, Energy & Minerals Science Center, Kansas Water Science Center, Oklahoma-Texas Water Science Center, South Atlantic Water Science Center (SAWSC), Upper Midwest Water Science Center, Reston Biogeochemical Processes in Groundwater LaboratoryWild bee exposure to pesticides in conservation grasslands increases along an agricultural gradient: A tale of two sample types
Conservation efforts have been implemented in agroecosystems to enhance pollinator diversity by creating grassland habitat, but little is known about the exposure of bees to pesticides while foraging in these grassland fields. Pesticide exposure was assessed in 24 conservation grassland fields along an agricultural gradient at two time points (July and August) using silicone band passive samplersAuthorsMichelle Hladik, Johanna M. Kraus, Cassandra Smith, Mark W. Vandever, Dana W. Kolpin, Carrie E Givens, Kelly SmallingPesticide prioritization by potential biological effects in tributaries of the Laurentian Great Lakes
Watersheds of the Great Lakes Basin (USA/Canada) are highly modified and impacted by human activities including pesticide use. Despite labeling restrictions intended to minimize risks to nontarget organisms, concerns remain that environmental exposures to pesticides may be occurring at levels negatively impacting nontarget organisms. We used a combination of organismal-level toxicity estimates (inAuthorsSamantha K. Oliver, Steven R. Corsi, Austin K. Baldwin, Michelle A. Nott, Gerald T. Ankley, Brett R. Blackwell, Daniel L. Villeneuve, Michelle Hladik, Dana W. Kolpin, Luke C. Loken, Laura A. DeCicco, Michael T. Meyer, Keith LoftinBottled water contaminant exposures and potential human effects
Bottled water (BW) consumption in the United States and globally has increased amidst heightened concern about environmental contaminant exposures and health risks in drinking water supplies, despite a paucity of directly comparable, environmentally-relevant contaminant exposure data for BW. This study provides insight into exposures and cumulative risks to human health from inorganic/organic/micrAuthorsPaul Bradley, Kristin Romanok, Kelly Smalling, Michael J. Focazio, Nicola Evans, Suzanne C. Fitzpatrick, Carrie E Givens, Stephanie Gordon, James L. Gray, Emily M. Green, Dale W. Griffin, Michelle Hladik, Leslie K. Kanagy, John T. Lisle, Keith Loftin, R. Blaine McCleskey, Elizabeth Medlock-Kakaley, Ana Navas-Acien, David A. Roth, Paul F. South, Christopher P. Weis - News