Jill Jenkins, Ph.D.
Ph.D., Microbiology, Memphis State University, 1991
M.S., Microbiology and Biochemistry, Idaho State University, 1985
B.S., Biology, Rochester Institute of Technology, 1981
Jill Jenkins’ efforts emphasize the study of potential impacts of environmental stressors at the cellular and molecular levels. Her major areas of focus include biomarker development, endocrine disruption, genetics, and comparative immunology. Key laboratory biotechnologies she uses are photomicroscopy and flow cytometry. Primary cell types studied are blood and spermatozoa, and assays are tailored to the particular species requirements and hypotheses being addressed.
Jenkins specializes in the development and application of biomarkers; these are measures of variables that respond in quantifiable ways to changes in the environment. The choice of particular biomarkers at specific biological levels of organization (such as genetic, cellular, tissue, hormonal, and organismal levels) depends on the study at hand. Because molecular and biochemical responses of cells are preceded by chemical changes in nuclei, cytoplasm, membranes, and extracellular fluids, these responses can be diagnostic and contribute to an assessment of ecosystem health.
1991-1992: Postdoctoral Res.; Reproductive Genetics Labs, Dept. of Obstetrics and Gynecology, Univ. of TN, with Stephen Wachtel
Science and Products
The objective of this research is to assess the sensitivity of female and male LMB reproductive capabilities at the time of year when they are physiologically preparing for spawning season (also known as gonad recrudescence).
The objectives of this study are to (1) investigate whether the immune system will respond to chemical stressors, such as new flame retardant compounds, and (2) determine if such chemical stressors influence white blood cells (WBC) responses after exposure to a viral pathogen mimicking bird flu.
Each year, the Buffalo National River (BUFF) attracts 1.6 million visitors, many of whom enjoy recreational water activities. Since 2013, a confined animal feeding operation (CAFO) for swine has been operating on Big Creek, a BUFF tributary.
Differentiating diploids from triploids at the earliest life stage possible allows for a more efficient use of resources including production time and rearing space. Thus, a reliable flow cytometric (FCM) method has been developed to discriminate triploids from diploids at the larval stage. In order to help simplify the process of differentiating triploids from diploids, we propose a simple...
Flow cytometry is a technique for rapidly analyzing large numbers of animal cells using light-scattering, fluorescence, and absorbance measurements.
Determining the Ploidy and Resultant Reproductive Capability of Artificially Spawned and Wild Caught Asian Carp
The invasive grass carp and black carp are artificially spawned to produce triploids, which means they have three sets of chromosomes and are sterile. WARC scientists invented an early ploidy prediction process for produced fry, and a post-mortem assessment method for carp caught either in the wild or sold and hauled live to other states.
Honey bee colony failure is primarily due to the infestation of mites and agricultural pesticides, including neonicotinoids. USGS researches the impacts these have on honey bee reproductive capabilities.
Neonicotinoid insecticides the fastest growing insecticide worldwide - and may play a role in the declines of terrestrial and aquatic vertebrate species.
Assessing the risk of diploid grass carp Ctenopharyngodon idella in the certified triploid supply chain in Ohio
Non-native grass carp (Ctenopharyngodon idella) have been stocked in the United States for vegetation control since the 1970s, and recent evidence suggests some natural reproduction in the Great Lakes basin. Despite all states and provinces bordering Lake Erie either banning grass carp or requiring imports of only sterile, U.S. Fish and Wildlife...Kinter, Bryan T.; Jenkins, Jill A.; Tyson, Jeff T.
Sperm quality biomarkers complement reproductive and endocrine parameters in investigating environmental contaminants in common carp (Cyprinus carpio) from the Lake Mead National Recreation Area
Lake Mead National Recreational Area (LMNRA) serves as critical habitat for several federally listed species and supplies water for municipal, domestic, and agricultural use in the Southwestern U.S. Contaminant sources and concentrations vary among the sub-basins within LMNRA. To investigate whether exposure to environmental contaminants is...Jenkins, Jill A.; Rosen, Michael R.; Dale, Rassa O.; Echols, Kathy R.; Torres, Leticia; Wieser, Carla M.; Kersten, Constance A.; Goodbred, S.
An accurate method for measuring triploidy of larval fish spawns
A standard flow cytometric protocol was developed for estimating triploid induction in batches of larval fish. Polyploid induction treatments are not guaranteed to be 100% efficient, thus the ability to quantify the proportion of triploid larvae generated by a particular treatment helps managers to stock high-percentage spawns and researchers to...Jenkins, Jill A.; Draugelis-Dale, Rassa O.; Glennon, Robert; Kelly, Anita; Brown, Bonnie L.; Morrison, John
A pilot study testing a natural and a synthetic Molluscicide for controlling invasive apple snails (Pomacea maculata)
Pomacea maculata (formerly P. insularum), an apple snail native to South America, was discovered in Louisiana in 2008. These snails strip vegetation, reproduce at tremendous rates, and have reduced rice production and caused ecosystem changes in Asia. In this pilot study snails were exposed to two molluscicides, a tea (Camellia sinensis...Olivier, Heather M.; Jenkins, Jill A.; Berhow, Mark; Carter, Jacoby
Hyla chrysoscelis (Cope’s gray treefrog) x Hyla cinerea (green treefrog): putative natural hybrid
Naturally–occurring hybrid treefrogs have been occasionally found in the eastern United States. However, these hybrids are almost always between members of the same species group. On 10 Jun 2014, at 2145 h, we located an individual making an unusual advertisement call along Bayou Manual Road in Sherburne Wildlife Management Area in the...Glorioso, Brad M.; Waddle, J. Hardin; Jenkins, Jill A.; Olivier, Heather M.; Layton, Rebekah R.
Novel associations between contaminant body burdens and biomarkers of reproductive condition in male Common Carp along multiple gradients of contaminant exposure in Lake Mead National Recreation Area, USA
Adult male Common Carp were sampled in 2007/08 over a full reproductive cycle at Lake Mead National Recreation Area. Sites sampled included a stream dominated by treated wastewater effluent, a lake basin receiving the streamflow, an upstream lake basin (reference), and a site below Hoover Dam. Individual body burdens for 252 contaminants were...Patino, Reynaldo; VanLandeghem, Matthew M.; Goodbred, Steven L.; Orsak, Erik; Jenkins, Jill A.; Echols, Kathy R.; Rosen, Michael R.; Torres, Leticia
Are endocrine and reproductive biomarkers altered in contaminant-exposed wild male Largemouth Bass (Micropterus salmoides) of Lake Mead, Nevada/Arizona, USA?
Male Largemouth Bass were sampled from two locations in Lake Mead (USA), a site influenced by treated municipal wastewater effluent and urban runoff (Las Vegas Bay), and a reference site (Overton Arm). Samples were collected in summer (July '07) and spring (March '08) to assess general health, endocrine and reproductive biomarkers, and compare...Goodbred, Steven L.; Patino, Reynaldo; Torres, Leticia; Echols, Kathy R.; Jenkins, Jill A.; Rosen, Michael R.; Orsak, Erik
Flow cytometric method for measuring chromatin fragmentation in fixed sperm from yellow perch (Perca flavescens)
Declining harvests of yellow perch, Perca flavescens, in urbanized watersheds of Chesapeake Bay have prompted investigations of their reproductive fitness. The purpose of this study was to establish a flow cytometric technique for DNA analysis of fixed samples sent from the field to provide reliable gamete quality measurements. Similar to the...Jenkins, Jill A.; Draugelis-Dale, Rassa O.; Pinkney, Alfred E.; Iwanowicz, Luke R.; Blazer, Vicki
Guidelines for use of fishes in research: revised and expanded
The Guidelines for the Use of Fishes in Research (2014; 2014 Guidelines), now available through the American fisheries Society (AFS) website and in print from the AFS bookstore, is a resource to aid researchers and regulatory authorities regarding responsible, scientifically valid research on fish and aquatic wildlife. The Guidelines for the Use...Jenkins, Jill A.; Bart, Henry L.; Bowker, James D.; Bowser, Paul R.; MacMillan, J. Randy; Nickum, John G.; Rachlin, Joseph W.; Rose, James D.; Sorensen, Peter W.; Warkentine, Barbara E.; Whitledge, Greg W.
Grass carp in the Great Lakes region: establishment potential, expert perceptions, and re-evaluation of experimental evidence of ecological impact
Intentional introductions of nonindigenous fishes are increasing globally. While benefits of these introductions are easily quantified, assessments to understand the negative impacts to ecosystems are often difficult, incomplete, or absent. Grass carp (Ctenopharyngodon idella) was originally introduced to the United States as a biocontrol agent,...Wittmann, Marion E.; Jerde, Christopher L.; Howeth, Jennifer G.; Maher, Sean P.; Deines, Andrew M.; Jenkins, Jill A.; Whitledge, Gregory W.; Burbank, Sarah B.; Chadderton, William L.; Mahon, Andrew R.; Tyson, Jeffrey T.; Gantz, Crysta A.; Keller, Reuben P.; Drake, John M.; Lodge, David M.
Assessing reproductive and endocrine parameters in male largescale suckers (Catostomus macrocheilus) along a contaminant gradient in the lower Columbia River, USA
Persistent organochlorine pollutants such as polychlorinated biphenyls (PCBs), dichlorodiphenyldichloroethylene (p,p′-DDE), and polybrominated diphenyl ethers (PBDEs) are stable, bioaccumulative, and widely found in the environment, wildlife, and the human population. To explore the hypothesis that reproduction in male fish is associated with...Jenkins, Jill A.; Olivier, H.M.; Draugelis-Dale, R. O.; Eilts, B.E.; Torres, L.; Patiño, R.; Nilsen, Elena B.; Goodbred, Steven L.
Development of a novel flow cytometric approach to evaluate fish sperm chromatin using fixed samples
The integrity of the paternal DNA is essential for the accurate transmission of genetic information, yet fertilization is not inhibited by chromatin breakage. Some methods are available for the sensitive detection of DNA damage and can be applied in studies of environmental toxicology, carcinogenesis, aging, and assisted reproduction techniques in...Jenkins, Jill A.